Abstract 1072: DNA methylation profile analysis of gliomas revealed a change in methylation status during malignant progression

2015 ◽  
Author(s):  
Mukasa Akitake ◽  
Kuniaki Saito ◽  
Koki Aihara ◽  
Genta Nagae ◽  
Mayu Omata ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Profile Analysis ◽  
Methylation Status ◽  
Methylation Profile ◽  
Malignant Progression ◽  
Dna Methylation Profile

scholarly journals Genome‐wide DNA methylation profile analysis in thoracic ossification of the ligamentum flavum

2020 ◽  
Vol 24 (15) ◽  
pp. 8753-8762
Author(s):  
Tianqi Fan ◽  
Xiangyu Meng ◽  
Chuiguo Sun ◽  
Xiaoxi Yang ◽  
Guanghui Chen ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Ligamentum Flavum ◽  
Profile Analysis ◽  
Methylation Profile ◽  
Genome Wide ◽  
Dna Methylation Profile

scholarly journals Association Between miR-148a and DNA Methylation Profile in Individuals Exposed to Lead (Pb)

2021 ◽  
Vol 12 ◽  
Author(s):  
Marília Ladeira de Araújo ◽  
Bruno Costa Gomes ◽  
Paula Pícoli Devóz ◽  
Nathália de Assis Aguilar Duarte ◽  
Diego Luis Ribeiro ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Methylation Status ◽  
Epidemiologic Studies ◽  
Methylation Profile ◽  
Taqman Assay ◽  
Inverse Association ◽  
Dna Methylation Profile ◽  
Lead Levels ◽  
Mirnas Expression ◽  
The Impact

Experimental and epidemiologic studies have shown that lead (Pb) is able to induce epigenetic modifications, such as changes in DNA methylation profiles, in chromatin remodeling, as well as the expression of non-coding RNAs (ncRNAs). However, very little is known about the interactions between microRNAs (miRNAs) expression and DNA methylation status in individuals exposed to the metal. The aim of the present study was to investigate the impact of hsa-miR-148a expression on DNA methylation status, in 85 workers exposed to Pb. Blood and plasma lead levels (BLL and PLL, respectively) were determined by ICP-MS; expression of the miRNA-148a was quantified by RT-qPCR (TaqMan assay) and assessment of the global DNA methylation profile (by measurement of 5-methylcytosine; % 5-mC) was performed by ELISA. An inverse association was seen between miR-148a and % 5-mC DNA, as a function of BLL and PLL (β = −3.7; p = 0.071 and β = −4.1; p = 0.049, respectively) adjusted for age, BMI, smoking, and alcohol consumption. Taken together, our study provides further evidence concerning the interactions between DNA methylation profile and miR-148a, in individuals exposed to Pb.

The DNA methylation profile of oocytes in mice with hyperinsulinaemia and hyperandrogenism as detected by single-cell level whole genome bisulphite sequencing (SC-WGBS) technology

2018 ◽  
Vol 30 (12) ◽  
pp. 1713 ◽  
Author(s):  
Qian-Nan Li ◽  
Lei Guo ◽  
Yi Hou ◽  
Xiang-Hong Ou ◽  
Zhonghua Liu ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Single Cell ◽  
Familial Aggregation ◽  
Methylation Status ◽  
Methylation Profile ◽  
Whole Genome ◽  
Single Cell Level ◽  
Cell Level ◽  
Bisulphite Sequencing ◽  
Dna Methylation Profile

Polycystic ovary syndrome (PCOS), a familial aggregation disease that causes anovulation in women, has well-recognised characteristics, two of which are hyperinsulinaemia and hyperandrogenaemia. To determine whether the DNA methylation status is altered in oocytes by high insulin and androgen levels, we generated a mouse model with hyperinsulinaemia and hyperandrogenaemia by injection of insulin and human chorionic gonadotrophin and investigated DNA methylation changes through single-cell level whole genome bisulphite sequencing. Our results showed that hyperinsulinaemia and hyperandrogenaemia had no significant effects on the global DNA methylation profile and different functional regions of genes, but did alter methylation status of some genes, which were significantly enriched in 17 gene ontology (GO) terms (P < 0.05) by GO analysis. Among differently methylated genes, some were related to the occurrence of PCOS. Based on our results, we suggest that hyperinsulinaemia and hyperandrogenaemia may cause changes in some DNA methylation loci in oocytes.

scholarly journals Integrated analysis of DNA methylation profile in HLA-G gene and imaging in coronary heart disease

2020 ◽  
Vol 41 (Supplement_2) ◽  
Author(s):  
G Benincasa ◽  
C Schiano ◽  
T Infante ◽  
M Franzese ◽  
R Casale ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Coronary Heart Disease ◽  
Heart Disease ◽  
Cpg Island ◽  
Methylation Profile ◽  
Integrated Analysis ◽  
Funding Source ◽  
Relevant Parameter ◽  
Cardiac Computed Tomography Angiography ◽  
Dna Methylation Profile

Abstract Aims Immune endothelial inflammation, underlie coronary heart disease (CHD) related phenotypes, could provide new insight into the pathobiology of the disease. We investigated DNA methylation level of the unique CpG island of HLA-G gene in CHD patients and evaluated the correlation with cardiac computed tomography angiography (CCTA) features. Methods Thirty-two patients that underwent CCTA for suspected CHD were enrolled for this study. Obstructive CHD group included fourteen patients, in which there was a stenosis greater than or equal to 50% in one or more of the major coronary arteries detected; whereas subjects with Calcium (Ca) Score=0, uninjured coronaries and with no obstructive CHD were considered as control subjects (Ctrls) (n=18). For both groups, DNA methylation profile of the whole 5'UTR-CpG island of HLA-G was measured. The plasma soluble HLA-G (sHLA-G) levels were detected in all subjects by specific ELISA assay. Statistical analysis was performed using R software. Results For the first time, our study reported that 1) a significant hypomethylation characterized three specific fragments (B, C and F) of the 5'UTR-CpG island (p=0.05) of HLA-G gene in CHD patients compared to Ctrl group; 2) hypomethylation level of one specific fragment positively correlated with coronary Ca score, a relevant parameter of CCTA (p&lt;0.05) between two groups. Conclusions Our results showed that reduced levels of circulating HLA-G molecules could derive from epigenetic marks inducing hypomethylation of specific regions into 5'UTR-CpG island of HLA-G gene in CHD patients with obstructive coronary stenosis vs non critical stenosis group. Funding Acknowledgement Type of funding source: Public grant(s) – National budget only. Main funding source(s): Italian Minister of Health

scholarly journals DNA methylation patterns identify subgroups of pancreatic neuroendocrine tumors with clinical association

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Vanessa Lakis ◽  
◽  
Rita T. Lawlor ◽  
Felicity Newell ◽  
Ann-Marie Patch ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Neuroendocrine Tumors ◽  
Methylation Profile ◽  
Pancreatic Neuroendocrine Tumors ◽  
Wild Type ◽  
Genomic Information ◽  
Chromosome 11 ◽  
Dna Methylation Profile ◽  
Methylation Patterns ◽  
Recurrent Patterns

AbstractHere we report the DNA methylation profile of 84 sporadic pancreatic neuroendocrine tumors (PanNETs) with associated clinical and genomic information. We identified three subgroups of PanNETs, termed T1, T2 and T3, with distinct patterns of methylation. The T1 subgroup was enriched for functional tumors and ATRX, DAXX and MEN1 wild-type genotypes. The T2 subgroup contained tumors with mutations in ATRX, DAXX and MEN1 and recurrent patterns of chromosomal losses in half of the genome with no association between regions with recurrent loss and methylation levels. T2 tumors were larger and had lower methylation in the MGMT gene body, which showed positive correlation with gene expression. The T3 subgroup harboured mutations in MEN1 with recurrent loss of chromosome 11, was enriched for grade G1 tumors and showed histological parameters associated with better prognosis. Our results suggest a role for methylation in both driving tumorigenesis and potentially stratifying prognosis in PanNETs.

scholarly journals DNA methylation profile of genes involved in inflammation and autoimmunity correlates with vascular function in morbidly obese adults

Epigenetics ◽  
2021 ◽  
pp. 1-17
Author(s):  
Mohamed M. Ali ◽  
Dina Naquiallah ◽  
Maryam Qureshi ◽  
Mohammed Imaduddin Mirza ◽  
Chandra Hassan ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Vascular Function ◽  
Methylation Profile ◽  
Morbidly Obese ◽  
Obese Adults ◽  
Dna Methylation Profile

Overlapping DNA methylation profile between placentas with trisomy 16 and early-onset preeclampsia

Placenta ◽  
2014 ◽  
Vol 35 (3) ◽  
pp. 216-222 ◽  
Author(s):  
J.D. Blair ◽  
S. Langlois ◽  
D.E. McFadden ◽  
W.P. Robinson
Keyword(s):  
Dna Methylation ◽  
Early Onset ◽  
Methylation Profile ◽  
Trisomy 16 ◽  
Dna Methylation Profile ◽  
Early Onset Preeclampsia
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