Abstract 4879: Cancer cell-mediated signaling of TLR 2, 4, and 9 causes activation of PI3K/Akt/mTOR pathway and induces tumor cell proliferation in pancreatic cancer

BackgroundRecurrence of liver metastasis after pancreatectomy is often a predictor of poor prognosis. Comprehensive genomic analysis may contribute to a better understanding of the molecular mechanisms of postoperative liver metastasis and provide new therapeutic targets.MethodsA total of 67 patients from The Cancer Genome Atlas (TCGA) were included in this study. We analyzed differentially expressed genes (DEGs) by R package “DESeq2.” Weighted gene co-expression network analysis (WGCNA) was applied to investigate the key modules and hub genes. Immunohistochemistry was used to analyze tumor cell proliferation index and CD4+ T cells infiltration.ResultsFunctional analysis of DEGs between the liver metastatic and recurrence-free groups was mainly concentrated in the immune response. The liver metastasis group had lower immune and stroma scores and a higher TP53 mutation rate. WGCNA showed that the genes in key modules related to disease-free survival (DFS) and overall survival (OS) were mainly enriched in the cell proliferation process and tumor immune response. Immunohistochemical analysis showed that the pancreatic cancer cells of patients with early postoperative liver metastasis had higher proliferative activity, while the infiltration of CD4+ T cells in tumor specimens was less.ConclusionOur study suggested that increased immune cell infiltration (especially CD4+ T cells) and tumor cell proliferation may play an opposite role in liver metastasis recurrence after pancreatic cancer.

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Inhibition of Enhancer of Zeste Homolog 2 (EZH2) Expression Is Associated With Decreased Tumor Cell Proliferation, Migration, and Invasion in Endometrial Cancer Cell Lines

International Journal of Gynecological Cancer ◽

10.1097/igc.0b013e318296a265 ◽

2013 ◽

Vol 23 (6) ◽

pp. 997-1005 ◽

Cited By ~ 38

Author(s):

Ramez N. Eskander ◽

Tao Ji ◽

Be Huynh ◽

Rooba Wardeh ◽

Leslie M. Randall ◽

...

Keyword(s):

Cell Proliferation ◽

Endometrial Cancer ◽

Tumor Cell ◽

Cell Lines ◽

Cancer Cell ◽

Cancer Cell Lines ◽

Migration And Invasion ◽

Tumor Cell Proliferation ◽

Endometrial Cancer Cell ◽

Ezh2 Expression

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WNT5A is a potent modulator of tumor cell proliferation and invasion overexpressed in pancreatic cancer

Zeitschrift für Gastroenterologie ◽

10.1055/s-2005-920090 ◽

2005 ◽

Vol 43 (05) ◽

Author(s):

P Michl ◽

S Ripka ◽

B Knobel ◽

G Adler ◽

TM Gress

Keyword(s):

Pancreatic Cancer ◽

Cell Proliferation ◽

Tumor Cell ◽

Tumor Cell Proliferation ◽

Proliferation And Invasion

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Inhibition of enhancer of zeste homolog 2 (EZH2) expression is associated with decreased tumor cell proliferation, migration and invasion in endometrial cancer cell lines

Gynecologic Oncology ◽

10.1016/j.ygyno.2012.07.014 ◽

2012 ◽

Vol 127 (1) ◽

pp. S5

Author(s):

R. Eskander ◽

T. Ji ◽

B. Huynh ◽

R. Wardeh ◽

B. Hoang ◽

...

Keyword(s):

Cell Proliferation ◽

Endometrial Cancer ◽

Tumor Cell ◽

Cell Lines ◽

Cancer Cell ◽

Cancer Cell Lines ◽

Migration And Invasion ◽

Tumor Cell Proliferation ◽

Endometrial Cancer Cell ◽

Ezh2 Expression

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Lipid Peroxidation, Cyclooxygenase Enzyme and Tumor Cell Proliferation Inhibitory Lignans from Justicia Species

Natural Product Communications ◽

10.1177/1934578x0800301109 ◽

2008 ◽

Vol 3 (11) ◽

pp. 1934578X0800301

Author(s):

Vanisree Mulabagala ◽

Gottumukkala V. Subbaraju ◽

Modukuri V. Ramani ◽

David L. DeWitt ◽

Muraleedharan G. Nair

Keyword(s):

Breast Cancer ◽

Lipid Peroxidation ◽

Cell Proliferation ◽

Tumor Cell ◽

Cell Lines ◽

Cancer Cell ◽

Breast Cancer Cell ◽

Breast Cancer Cell Lines ◽

Tumor Cell Proliferation ◽

Cox 1

The genus Justicia is a rich source of lignans, especially aryl naphthalide lignans. Lignans are biologically active phytochemicals, and are reported to possess antiplatelet, antiviral, anti-tumor, antidepressant, and insect antifeedant activities. In the present study, we report lipid peroxidation (LPO), cyclooxygenase (COX-1 and −2) enzyme and tumor cell proliferation inhibitory activities of lignans, namely, lariciresinol (1), isolariciresinol (2), neesiinoside A (3), justirumalin (4), justalakonin (5), justicidin G (6), sesamin (7), sesamolin (8), jusmicranthin methyl ether (9), taiwanin E methyl ether (10), lignan J1 (11), jusneesiinol (12), jusmicranthin ethyl ether (13), tiruneesiin (14), justicidin E (15) and simplexolin (16). Lignans 1 and 2 were isolated from J. tranquebariensis, 3, 4, 6, 9, 10, 11, 12, 13 and 14 from J. neesii Ramamoorthy, 5 from J. purpurea and 7, 8, 15 and 16 from J. orbiculata. Among the lignans assayed, 1, 2, 12 and 14 showed 79.6, 86.2, 90.8 and 95.9% and 3 41.3% inhibition of LPO at 25 μg/mL. The lignans 4, 9 and 16 inhibited COX-2 enzyme by 67.2, 73.0 and 72.8%, respectively, when tested at 25 μg/mL. Similarly, lignans 3, 4, 10, 11 and 15 inhibited COX-1 enzyme by 59.9, 89.2, 69.6, 73.9, and 80.1%, respectively, at 25 μg/mL. When assayed at 25 μg/mL, 4 inhibited human stomach and breast cancer cell lines by 42.8 and 42.1%, respectively. Also, at 25 μg/mL the lignan 7 inhibited the growth of CNS, lung and breast cancer cell lines by 50.0, 41.3, and 42.0 %, respectively, and 15 inhibited the proliferation of lung, breast and colon cell lines by 40–53%.

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