Abstract 3784: Expression of estrogen receptor specific signaling transcriptome in epithelial cell adhesion molecule (EpCAM) captured circulating tumor cells from patients with breast cancer

2017 ◽  
Author(s):  
Ashok Singh ◽  
Jamie M. Sperger ◽  
Jennifer Schehr ◽  
Tessa Witkowski ◽  
Beth A. Weaver ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Estrogen Receptor ◽  
Cell Adhesion ◽  
Epithelial Cell ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Adhesion Molecule ◽  
Cell Adhesion Molecule ◽  
Epithelial Cell Adhesion Molecule

Demasking of epithelial cell adhesion molecule (EpCAM) on circulating epithelial tumor cells by Tween®20 treatment in breast cancer patients

2012 ◽  
Vol 50 (4) ◽  
Author(s):  
Katya Hekimian ◽  
Ernst-Ludwig Stein ◽  
Ulrich Pachmann ◽  
Katharina Pachmann
Keyword(s):  
Breast Cancer ◽  
Immune Response ◽  
Cell Adhesion ◽  
Epithelial Cell ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Cell Adhesion Molecule ◽  
Breast Cancer Patients ◽  
Epithelial Cell Adhesion Molecule ◽  
Epithelial Tumor

AbstractThe epithelial cell adhesion molecule (EpCAM) embedded in the plasma membrane of circulating epithelial tumor cells (CETC) is used for detection and enrichment of circulating tumor cells in peripheral blood and as a target for anti-epithelial antibodies elicited during immune response in anti-tumor immunization. Although an efficient immune response against EpCAM can be generated, the clinical application of such approaches has not been successful so far and the detection of circulating epithelial cells is highly variable. One reason for these discrepancies may be that not all circulating tumor cells are equally accessible for the specific antibody. A possible reason might be masking of EpCAM by glycoproteins or membrane lipoproteins preventing antibody binding.We have tested the application of detergents as demasking agents known to be successful in demasking red blood cell epitopes and determined how and in which way they affect integral membrane proteins and membrane lipids.The results showed that the polysorbate TweenThe data presented in this study suggest that EpCAM is present on part of circulating tumor cells in a masked form and that it is possible to demask EpCAM on CETC of breast cancer patients using Tween

scholarly journals Quick chip assay using locked nucleic acid modified epithelial cell adhesion molecule and nucleolin aptamers for the capture of circulating tumor cells

2015 ◽  
Vol 9 (5) ◽  
pp. 054110 ◽  
Author(s):  
Nihal G. Maremanda ◽  
Kislay Roy ◽  
Rupinder K. Kanwar ◽  
Vidyarani Shyamsundar ◽  
Vijayalakshmi Ramshankar ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Nucleic Acid ◽  
Epithelial Cell ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Adhesion Molecule ◽  
Cell Adhesion Molecule ◽  
Locked Nucleic Acid ◽  
Chip Assay ◽  
Epithelial Cell Adhesion Molecule

Epithelial Cell Adhesion Molecule-positive Circulating Tumor Cells as Predictive Biomarker in Patients With Prostate Cancer

Urology ◽  
2013 ◽  
Vol 81 (6) ◽  
pp. 1303-1307 ◽  
Author(s):  
Robert J. Amato ◽  
Vladislava Melnikova ◽  
Yujian Zhang ◽  
Wen Liu ◽  
Somyata Saxena ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Cell Adhesion ◽  
Epithelial Cell ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Adhesion Molecule ◽  
Cell Adhesion Molecule ◽  
Predictive Biomarker ◽  
Epithelial Cell Adhesion Molecule

scholarly journals Epithelial Cell Adhesion Molecule: An Anchor to Isolate Clinically Relevant Circulating Tumor Cells

Cells ◽  
2020 ◽  
Vol 9 (8) ◽  
pp. 1836
Author(s):  
Zahra Eslami-S ◽  
Luis Enrique Cortés-Hernández ◽  
Catherine Alix-Panabières
Keyword(s):  
Cell Adhesion ◽  
Epithelial Cell ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Adhesion Molecule ◽  
Cell Adhesion Molecule ◽  
Epithelial To Mesenchymal Transition ◽  
Epithelial Cell Adhesion Molecule ◽  
Mesenchymal Transition ◽  
Advantages And Disadvantages

In the last few decades, the epithelial cell adhesion molecule (EpCAM) has received increased attention as the main membrane marker used in many enrichment technologies to isolate circulating tumor cells (CTCs). Although there has been a great deal of progress in the implementation of EpCAM-based CTC detection technologies in medical settings, several issues continue to limit their clinical utility. The biology of EpCAM and its role are not completely understood but evidence suggests that the expression of this epithelial cell-surface protein is crucial for metastasis-competent CTCs and may not be lost completely during the epithelial-to-mesenchymal transition. In this review, we summarize the most significant advantages and disadvantages of using EpCAM as a marker for CTC enrichment and its potential biological role in the metastatic cascade.

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