Abstract 212: Sarcoma-CellMiner: An extensive resource for patient-derived sarcoma cell line epigenetics, genomics and pharmacology

2021 ◽  
Author(s):  
Camille Tlemsani ◽  
Lorinc Pongor ◽  
Javed Khan ◽  
Fathi Elloumi ◽  
Sudhir Varma ◽  
...  
Keyword(s):  
Cell Line ◽  
Sarcoma Cell ◽  
Sarcoma Cell Line

Modulation of glucose transport by parathyroid hormone and insulin in UMR 106–01, a clonal rat osteogenic sarcoma cell line

1995 ◽  
Vol 14 (2) ◽  
pp. 263-275 ◽  
Author(s):  
D M Thomas ◽  
S D Rogers ◽  
M W Sleeman ◽  
G M Pasquini ◽  
F R Bringhurst ◽  
...  
Keyword(s):  
Parathyroid Hormone ◽  
Cell Line ◽  
Glucose Transport ◽  
Transport System ◽  
Osteogenic Sarcoma ◽  
Regulatory Subunit ◽  
Sarcoma Cell ◽  
Sarcoma Cell Line ◽  
Glucose Transport System ◽  
Umr 106

ABSTRACT This study characterizes the actions of insulin and parathyroid hormone (PTH) on the glucose transport system in the rat osteogenic sarcoma cell line UMR 106–01, which expresses a number of features of the osteoblast phenotype. Using [1,2-3H]2-deoxyglucose (2-DOG) as a label, UMR 106–01 cells were shown to possess a glucose transport system which was enhanced by insulin. In contrast, PTH influenced glucose transport in a biphasic manner with a stimulatory effect at 1 h and a more potent inhibitory effect at 16 h on basal and insulin-stimulated 2-DOG transport. To explore the mechanism of PTH action, a direct agonist of cAMP-dependent protein kinase (PKA) was tested. 8-Bromo-cAMP had no acute stimulatory effect but inhibited basal and insulin-stimulated 2-DOG transport at 16 h. This result suggested that the prolonged, but not the acute, effect of PTH was mediated by the generation of cAMP. Further studies with the cell line UMR 4–7, a UMR 106–01 clone stably transfected with an inducible mutant inactive regulatory subunit of PKA, confirmed that the inhibitory but not the stimulatory effect of PTH was mediated by the PKA pathway. Northern blot data indicated that the prolonged inhibitory effects of PTH and 8-bromo-cAMP on glucose transport were likely to be mediated in part by reduction in the levels of GLUT1 (HepG2/brain glucose transporter) mRNA.

scholarly journals U-DCS: characterization of the first permanent human dendritic sarcoma cell line

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Kevin Mellert ◽  
Julian Benckendorff ◽  
Frank Leithäuser ◽  
Katarzyna Zimmermann ◽  
Peter Wiegand ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Dendritic Cell ◽  
Cell Line ◽  
S100 Protein ◽  
Sarcoma Cell ◽  
Vimentin Expression ◽  
Sarcoma Cell Line ◽  
Cell Sarcoma ◽  
Dendritic Cell Sarcoma ◽  
Functional Features

AbstractA dendritic cell sarcoma cell line, U-DCS, was established from a dendritic cell sarcoma in a 53-year-old Caucasian male patient. Since its establishment, U-DCS has maintained stable phenotypic characteristics in vitro and has a doubling time of approximately 2 days under standard culture conditions. U-DCS is growing with typical dendritic cell morphology in tissue and expresses the dendritic cell sarcoma immunophenotypic markers S100 protein, MHCI, MHCII, and vimentin. Expression analysis revealed transcripts for the toll-like receptors TLR3, -4, -9 and DDX58 (RIG-I), but not for TLR2. U-DCS shows functional features of dendritic cells with the ability of phagocytosis and antigen-specific T cell stimulation. Karyotype-, CGH-, and mFISH analysis point to a chromosomal instability and a hypotetraploid karyotype with approximately 130 chromosomes. U-DCS is the first immortalized human dendritic cell sarcoma cell line and has some morphological and functional features of dendritic cells without dependency on growth factors.

scholarly journals SMARCB1/INI1 Genetic Inactivation Is Responsible for Tumorigenic Properties of Epithelioid Sarcoma Cell Line VAESBJ

2013 ◽  
Vol 12 (6) ◽  
pp. 1060-1072 ◽  
Author(s):  
Monica Brenca ◽  
Sabrina Rossi ◽  
Erica Lorenzetto ◽  
Elena Piccinin ◽  
Sara Piccinin ◽  
...  
Keyword(s):  
Cell Line ◽  
Epithelioid Sarcoma ◽  
Sarcoma Cell ◽  
Sarcoma Cell Line ◽  
Genetic Inactivation

Radiosensitization of a human soft tissue sarcoma cell line US8-93 (mt-p53) with the oxidizer sodium peroxodisulfate

2004 ◽  
Author(s):  
Matthias Bache ◽  
Jürgen Dunst ◽  
Frank Matschiner ◽  
Sybille Matschiner ◽  
Matthias Kappler ◽  
...  
Keyword(s):  
Soft Tissue ◽  
Soft Tissue Sarcoma ◽  
Cell Line ◽  
Sarcoma Cell ◽  
Sarcoma Cell Line ◽  
Human Soft Tissue ◽  
Sodium Peroxodisulfate

Products from alkylation of DNA in cells by melphalan: human soft tissue sarcoma cell line RD and Escherichia coli WP2

1995 ◽  
Vol 97 (3) ◽  
pp. 287-296 ◽  
Author(s):  
Martin R. Osborne ◽  
Philip D. Lawley ◽  
Christopher Crofton-Sleigh ◽  
William Warren
Keyword(s):  
Escherichia Coli ◽  
Soft Tissue ◽  
Soft Tissue Sarcoma ◽  
Cell Line ◽  
Sarcoma Cell ◽  
Sarcoma Cell Line ◽  
Human Soft Tissue ◽  
In Cells
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