Conjugated Linoleic Acid - Mediated Apoptosis in Jurkat T Cells Involves the Production of Reactive Oxygen Species

2004 ◽  
Vol 14 (1-2) ◽  
pp. 57-64 ◽  
Author(s):  
Paolo Bergamo ◽  
Diomira Luongo ◽  
Mauro Rossi
Keyword(s):  
Reactive Oxygen Species ◽  
T Cells ◽  
Linoleic Acid ◽  
Conjugated Linoleic Acid ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Jurkat T Cells

Vibrio vulnificus-induced death of Jurkat T-cells requires activation of p38 mitogen-activated protein kinase by NADPH oxidase-derived reactive oxygen species

2008 ◽  
Vol 253 (1-2) ◽  
pp. 81-91 ◽  
Author(s):  
Woo Hyang Kim ◽  
Sung Young Goo ◽  
Myeong Heon Shin ◽  
Se-Jin Chun ◽  
Heuiran Lee ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
T Cells ◽  
Protein Kinase ◽  
Nadph Oxidase ◽  
Vibrio Vulnificus ◽  
Reactive Oxygen ◽  
Mitogen Activated Protein Kinase ◽  
Oxygen Species ◽  
Jurkat T Cells ◽  
Mitogen Activated Protein

Mechanism of cell death induced by an antioxidant extract of Cratoxylum cochinchinense (YCT) in Jurkat T cells: the role of reactive oxygen species and calcium

2004 ◽  
Vol 36 (12) ◽  
pp. 1588-1611 ◽  
Author(s):  
Soon Yew Tang ◽  
Matthew Whiteman ◽  
Andrew Jenner ◽  
Zhao Feng Peng ◽  
Barry Halliwell
Keyword(s):  
Reactive Oxygen Species ◽  
T Cells ◽  
Cell Death ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Jurkat T Cells

scholarly journals Ceramide induces a loss in cytosolic peroxide levels in mononuclear cells

2003 ◽  
Vol 375 (3) ◽  
pp. 567-579 ◽  
Author(s):  
Darren C. PHILLIPS ◽  
Helen R. GRIFFITHS
Keyword(s):  
Reactive Oxygen Species ◽  
T Cells ◽  
Cellular Response ◽  
Mononuclear Cells ◽  
Human Peripheral Blood ◽  
Membrane Integrity ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Jurkat T Cells ◽  
Acetyl Cysteine

Ceramide (a sphingolipid) and reactive oxygen species are each partly responsible for intracellular signal transduction in response to a variety of agents. It has been reported that ceramide and reactive oxygen species are intimately linked and show reciprocal regulation [Liu, Andreieu-Abadie, Levade, Zhang, Obeid and Hannun (1998) J. Biol. Chem. 273, 11313–11320]. Utilizing synthetic, short-chain ceramide to mimic the cellular responses to fluctuations in natural endogenous ceramide formation or using stimulation of CD95 to induce ceramide formation, we found that the principal redox-altering property of ceramide is to lower the [peroxide]cyt (cytosolic peroxide concentration). Apoptosis of Jurkat T-cells, primary resting and phytohaemagglutinin-activated human peripheral blood T-lymphocytes was preceded by a loss in [peroxide]cyt, as measured by the peroxide-sensitive probe 2′,7′-dichlorofluorescein diacetate (also reflected in a lower rate of superoxide dismutase-inhibitable cytochrome c reduction), and this was not associated with a loss of membrane integrity. Where growth arrest of U937 monocytes was observed without a loss of membrane integrity, the decrease in [peroxide]cyt was of a lower magnitude when compared with that preceding the onset of apoptosis in T-cells. Furthermore, decreasing the cytosolic peroxide level in U937 monocytes before the application of synthetic ceramide by pretreatment with either of the antioxidants N-acetyl cysteine or glutathione conferred apoptosis. However, N-acetyl cysteine or glutathione did not affect the kinetics or magnitude of ceramide-induced apoptosis of Jurkat T-cells. Therefore the primary redox effect of cellular ceramide accumulation is to lower the [peroxide]cyt of both primary and immortalized cells, the magnitude of which dictates the cellular response.

scholarly journals Conjugated linoleic acid, unlike other unsaturated fatty acids, strongly induces glutathione synthesis without any lipoperoxidation

2006 ◽  
Vol 96 (5) ◽  
pp. 811-819 ◽  
Author(s):  
Khelifa Arab ◽  
Adrien Rossary ◽  
Laurent Soulère ◽  
Jean-Paul Steghens
Keyword(s):  
Reactive Oxygen Species ◽  
Fatty Acids ◽  
Arachidonic Acid ◽  
Linoleic Acid ◽  
Conjugated Linoleic Acid ◽  
Reactive Oxygen ◽  
Redox Status ◽  
Cyclooxygenase 2 ◽  
Cytotoxicity Test ◽  
Oxygen Species

Enhancement of the redox status of cells is a cytoprotective strategy against oxidative damage. We recently showed that DHA upregulates glutathione (GSH) content via an induction of its related enzymes γ-glutamylcysteine ligase and glutathione reductase. In the present study, we investigated the effects of eight other fatty acids on the redox status and lipid peroxidation of human fibroblasts. After 48 h, only arachidonic acid and conjugated linoleic acid (CLA) enhanced GSH content through an induction of γ-glutamylcysteine ligase. CLA was more potent than arachidonic acid in inducing GSH synthesis. For all the fatty acids tested, lipoperoxidation, estimated by cell malondialdehyde measurement, did not differ from that of controls at 48 h but dramatically increased at 7 d, except for CLA. Lipoperoxidation is associated at 7 d with a high level of reactive oxygen species and with increased haemoxygenase-1 and cyclooxygenase-2 mRNA expression. As demonstrated by a tert-butylhydroperoxide cytotoxicity test, the GSH synthesis obtained with arachidonic acid is not sufficient to protect the cells, whereas this protective effect was obvious with CLA at 48 h as well as at 7 d. The present results show that CLA is the only PUFA able to induce GSH synthesis without any change in oxidative balance, whereas an upregulation of cyclooxygenase-2 by other PUFA is concomitant with an overproduction of malondialdehyde and reactive oxygen species. The particular hairpin conformation obtained for CLA by molecular modelling could account for this specific biological effect.

n-Hexane toxicity in Jurkat T-cells is mediated by reactive oxygen species

2008 ◽  
Vol 82 (3) ◽  
pp. 165-171 ◽  
Author(s):  
Catherine McDermott ◽  
Maria Hutch O’Donoghue ◽  
James J. A. Heffron
Keyword(s):  
Reactive Oxygen Species ◽  
T Cells ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Jurkat T Cells

Reactive oxygen species-dependent necroptosis in Jurkat T cells induced by pathogenic free-living Naegleria fowleri

2011 ◽  
Vol 33 (7) ◽  
pp. 390-400 ◽  
Author(s):  
K.-J. SONG ◽  
Y. S. JANG ◽  
Y. A. LEE ◽  
K. A. KIM ◽  
S. K. LEE ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
T Cells ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Naegleria Fowleri ◽  
Jurkat T Cells ◽  
Free Living ◽  
Nægleria Fowleri

Reactive oxygen species-mediated caspase-3 pathway involved in cell apoptosis of Karenia mikimotoi induced by linoleic acid

2018 ◽  
Vol 36 ◽  
pp. 48-56 ◽  
Author(s):  
Meiaoxue Han ◽  
Renjun Wang ◽  
Ning Ding ◽  
Xiuxia Liu ◽  
Ningning Zheng ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Linoleic Acid ◽  
Cell Apoptosis ◽  
Caspase 3 ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Karenia Mikimotoi

scholarly journals Induction of regulatory T cells by macrophages is dependent on production of reactive oxygen species

2010 ◽  
Vol 107 (41) ◽  
pp. 17686-17691 ◽  
Author(s):  
Marina D. Kraaij ◽  
Nigel D. L. Savage ◽  
Sandra W. van der Kooij ◽  
Karin Koekkoek ◽  
Jun Wang ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
T Cells ◽  
Regulatory T Cells ◽  
Reactive Oxygen ◽  
Oxygen Species

scholarly journals Excessive Reactive Oxygen Species Inhibit IL-17A+ γδ T Cells and Innate Cellular Responses to Bacterial Lung Infection

2020 ◽  
Vol 32 (13) ◽  
pp. 943-956
Author(s):  
Desiree Anthony ◽  
Angelica Papanicolaou ◽  
Hao Wang ◽  
Huei Jiunn Seow ◽  
Eunice E. To ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
T Cells ◽  
Reactive Oxygen ◽  
Γδ T Cells ◽  
Cellular Responses ◽  
Lung Infection ◽  
Oxygen Species
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