Detection by Polymerase Chain Reaction of all Common Mycoplasma in a Cell Culture Facility

Pathobiology ◽  
1995 ◽  
Vol 63 (1) ◽  
pp. 9-11 ◽  
Author(s):  
James M. Pruckler ◽  
Janet M. Pruckler ◽  
Edwin W. Ades
Keyword(s):  
Cell Culture ◽  
Polymerase Chain Reaction ◽  
Chain Reaction ◽  
A Cell ◽  
Polymerase Chain ◽  
Cell Culture Facility

Detection of poliovirus by cell culture and by PCR after UV disinfection

1995 ◽  
Vol 31 (5-6) ◽  
pp. 141-145 ◽  
Author(s):  
A. Maier ◽  
D. Tougianidou ◽  
A. Wiedenmann ◽  
K. Botzenhart
Keyword(s):  
Cell Culture ◽  
Polymerase Chain Reaction ◽  
Uv Irradiation ◽  
Viral Rna ◽  
Uv Disinfection ◽  
Mercury Lamp ◽  
Chain Reaction ◽  
A Cell ◽  
Polymerase Chain ◽  
Cell Culture Assay

The polymerase chain reaction (PCR) is used for the detection of microorganisms in water, but it is known that one does not detect only viable microorganisms by PCR. The following results show the detection of poliovirus on a cell culture assay and by PCR after inactivation by UV irradiation. The virus was irradiated with a low pressure mercury lamp with times up to 4000 seconds (=3980 J/m2). A 4 log inactivation of the virus was complete after 300 seconds (=294 J/m2) as shown by the cell culture assay. The viral RNA was detectable by PCR even at irradiation times of 4000 seconds.

An Effective Cell Coculture Platform Based on the Electrospun Microtube Array Membrane for Nerve Regeneration

2017 ◽  
Vol 204 (3-4) ◽  
pp. 179-190 ◽  
Author(s):  
V. Chia-Hsuan Tseng ◽  
Chee Ho Chew ◽  
Wan-Ting Huang ◽  
Yang-Kao Wang ◽  
Ko-Shao Chen ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Chain Reaction ◽  
Tissue Culture Polystyrene ◽  
Coculture System ◽  
Submicron Scale ◽  
A Cell ◽  
Polymerase Chain ◽  
Transwell System ◽  
Lower Expression ◽  
Microtube Array

Recently, a novel substrate known as an electrospun polylactic acid (PLLA) microtube array membrane (MTAM) was successfully developed as a cell coculture platform. Structurally, this substrate is made up of one-to-one connected, ultrathin, submicron scale fibers that are arranged in an arrayed formation. Its unique structure confers several key advantages which are beneficial in a cell coculture system. In this study, the interaction between rat fetal neural stem cells (NSC) and astrocytes was examined by comparing the outcome of a typical Transwell-based coculture system and that of an electrospun PLLA MTAM-based coculture system. Compared to tissue culture polystyrene (TCP) and Transwell coculture inserts, a superior cell viability of NSC was observed when cultured in lumens of electrospun PLLA MTAM (with supportive immunostaining images). Reverse transcription polymerase chain reaction revealed a strong interaction between astrocytes and NSC through a higher expression of doublecortin and a lower expression of nestin. These data demonstrate that MTAM is clearly a better coculture platform than the traditional Transwell system.

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