Clog-free and reliable solvent bonding of poly(methyl methacrylate) microdevice mediated by eco-friendly acetic acid at room temperature and its application for polymerase chain reaction and human cell culture

2019 ◽  
Vol 282 ◽  
pp. 1008-1017 ◽  
Author(s):  
Kieu The Loan Trinh ◽  
Quang Nghia Pham ◽  
Nae Yoon Lee
Keyword(s):  
Cell Culture ◽  
Polymerase Chain Reaction ◽  
Acetic Acid ◽  
Methyl Methacrylate ◽  
Human Cell ◽  
Room Temperature ◽  
Chain Reaction ◽  
Human Cell Culture ◽  
Polymerase Chain ◽  
Solvent Bonding

Early Detection of Systemic Rust Infections of Dyers Woad (Isatis tinctoria) Using the Polymerase Chain Reaction

Weed Science ◽  
1995 ◽  
Vol 43 (3) ◽  
pp. 467-472 ◽  
Author(s):  
Bradley R. Kropp ◽  
Steve Albee ◽  
Karen M. Flint ◽  
Paul Zambino ◽  
Les Szabo ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Early Detection ◽  
Room Temperature ◽  
Detection Method ◽  
Rust Fungus ◽  
Pcr Primers ◽  
Chain Reaction ◽  
Isatis Tinctoria ◽  
Specific Polymerase Chain Reaction ◽  
Polymerase Chain

Rust-specific polymerase chain reaction (PCR) primers selectively amplified ribosomal DNA of a rust fungus from infected dyers woad. PCR enabled DNA of the fungus to be detected in symptomatic plants as well as in asymptomatic parts of diseased plants. The use of PCR enabled early detection of rust infections in dyers woad plants during their first season when they are often asymptomatic Dried plant samples stored at room temperature for several months worked as well as lyophilized material for DNA extraction prior to PCR. The PCR detection method should greatly facilitate further studies on the biology and inoculation of this and other systemic rusts that have potential for use in biocontrol of weeds.

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