Relative frequencies of G (VP7) and P (VP4) serotypes determined by polymerase chain reaction assays among Japanese bovine rotaviruses isolated in cell culture.

1993 ◽  
Vol 31 (11) ◽  
pp. 3046-3049 ◽  
Author(s):  
Y Suzuki ◽  
T Sanekata ◽  
M Sato ◽  
K Tajima ◽  
Y Matsuda ◽  
...  
Keyword(s):  
Cell Culture ◽  
Polymerase Chain Reaction ◽  
Chain Reaction ◽  
Polymerase Chain

Persistent Measles Virus Infection and Otosclerosis

2001 ◽  
Vol 110 (10) ◽  
pp. 897-903 ◽  
Author(s):  
Wolfgang Arnold ◽  
Hans P. Niedermeyer ◽  
Maria Schuster ◽  
Wolfgang J. Neubert ◽  
Christa Baumann ◽  
...  
Keyword(s):  
Cell Culture ◽  
Polymerase Chain Reaction ◽  
Measles Virus ◽  
Otic Capsule ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Virus Rna ◽  
Polymerase Chain ◽  
H Protein ◽  
Antibody Pattern

The cause of otosclerosis is still unknown. Recently, measles virus involvement has been implicated. The aim of this study was to analyze the presence of measles virus RNA within the otosclerotic focus and to evaluate the perilymphatic antibody pattern. Bone and perilymph specimens from 40 patients with the spontaneous form of otosclerosis and from control patients were investigated by reverse transcription polymerase chain reaction (RT-PCR), Western blot techniques, and cell culture. By the use of RT-PCR, measles virus RNA could be detected in 32 patients, but not in controls. Analysis of perilymph revealed the presence of antibodies to N, F1, and M measles virus proteins in all cases, and antibodies against H protein in 2 additional cases. In preosteoblasts cultured from otosclerotic bone chips, no measles virus RNA could be amplified. We conclude that the spontaneous form of otosclerosis is, in the vast majority of cases, a measles virus-associated disease of the otic capsule.

Sign in / Sign up

Export Citation Format

Share Document