Honokiol Inhibits Vascular Vessel Formation of Mouse Embryonic Stem Cell-Derived Endothelial Cells via the Suppression of PECAM and MAPK/mTOR Signaling Pathway

Introduction: Nicotine is a key biologically active compound of cigarettes. Although nicotine is a risk factor for various health issues, it may also be beneficial when treated at moderate concentrations. Nicotine has been shown to bidirectionally regulate stem cell proliferation and differentiation depending on the doses applied. It is not clear whether or how nicotine regulates mouse embryonic stem cell (mESC) survival and proliferation. Methods: Mouse embryonic stem cells were cultured in the presence of 0.01, 0.1, 1, or 10 μM nicotine. The effects of nicotine on cell survival and proliferation were examined. The signaling pathway that mediated these effects was analyzed. Results: Cell viability was not affected by nicotine at all 4 concentrations examined. The proliferation of mESCs was promoted by 0.01 and 0.1 μM nicotine and suppressed by 1 and 10 μM. This dose-dependent regulation was mediated through the Wnt/β-catenin pathway. Modulation of Wnt/β-catenin activity either worsens or reverses the effects of nicotine. Conclusions: We have identified a bidirectional function of nicotine on mESC proliferation through regulation of the Wnt/β-catenin pathway and this is associated with different doses. This study suggests that concentration of nicotine is a crucial aspect for consideration when designing research or therapeutic strategies.

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Role of Notch1 in the arterial specification and angiogenic potential of mouse embryonic stem cell-derived endothelial cells

Stem Cell Research & Therapy ◽

10.1186/s13287-018-0945-7 ◽

2018 ◽

Vol 9 (1) ◽

Cited By ~ 5

Author(s):

Jae Kyung Park ◽

Tae Wook Lee ◽

Eun Kyoung Do ◽

Hye Ji Moon ◽

Jae Ho Kim

Keyword(s):

Endothelial Cells ◽

Stem Cell ◽

Embryonic Stem Cell ◽

Embryonic Stem ◽

Mouse Embryonic Stem Cell ◽

Angiogenic Potential

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Development of Hematopoietic and Endothelial Cells from Human Embryonic Stem Cells: Lessons from the Studies using Mouse as a Model

The Scientific World JOURNAL ◽

10.1100/tsw.2007.310 ◽

2007 ◽

Vol 7 ◽

pp. 1950-1964 ◽

Cited By ~ 2

Author(s):

Anna Jezierski ◽

Albert Swedani ◽

Lisheng Wang

Keyword(s):

Endothelial Cells ◽

Stem Cell ◽

Embryonic Stem Cell ◽

Cell Model ◽

Embryonic Stem ◽

Mouse Embryonic Stem Cell ◽

Model System ◽

Stem Cell Model ◽

Cell Model System ◽

Cellular Hierarchy

The current progress using the human embryonic stem cell (hESC) model system has provided much insight into the early origins of the hematopoietic and endothelial lineages, particularly the elusive hemangioblast. Recently, the cellular hierarchy and molecular regulation controlling hematopoietic commitment have been further elucidated. These findings not only provide new insights into early human development, but also advance the knowledge required to develop techniques capable of generating a given cell type for potential clinical applications. This review will focus on the latest advances using the hESC model system, capitalizing on the well-established mouse embryonic stem cell model system, as a means to investigate the lineage commitment events underlying the early embryonic development of human hematopoietic and endothelial cells.

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