The Impact of Gestational Age on Targeted Amniotic Cell Profiling in Experimental Neural Tube Defects

2014 ◽  
Vol 37 (1) ◽  
pp. 65-69 ◽  
Author(s):  
Elliot C. Pennington ◽  
Kristy L. Rialon ◽  
Beatrice Dionigi ◽  
Azra Ahmed ◽  
David Zurakowski ◽  
...  
Keyword(s):  
Stem Cells ◽  
Spina Bifida ◽  
Amniotic Fluid ◽  
Neural Tube ◽  
Neural Tube Defects ◽  
Gestational Age ◽  
Sprague Dawley ◽  
Amniotic Cell ◽  
The Impact ◽  
Wallis Anova

Purpose: The proportions of select stem cells in term amniotic fluid have been shown to correlate with the type and size of experimental neural tube defects (NTDs). We sought to determine the impact of gestational age upon this form of targeted amniotic cell profiling. Methods: Sprague-Dawley fetuses with retinoic acid-induced NTDs (n = 110) underwent amniotic fluid procurement at four time points in gestation. Samples were analyzed by flow cytometry for the presence of cells concomitantly expressing Nestin and Sox-2 (neural stem cells, aNSCs) and cells concomitantly expressing CD29 and CD44 (mesenchymal stem cells, aMSCs). Statistical analysis was by nonparametric Kruskal-Wallis ANOVA (p < 0.05). Results: There was a statistically significant impact of gestational age on the proportions of both aMSCs (p = 0.01) and aNSCs (p < 0.01) in fetuses with isolated spina bifida. No such impact was noted in normal fetuses (p > 0.10 for both cells), in isolated exencephaly (p > 0.10 for both cells), or in combination defects (p > 0.10 for both cells). Gestational age had no effect on aNSC/aMSC ratios. Conclusions: Targeted quantitative amniotic cell profiling varies with gestational age in experimental isolated spina bifida. This finding should be considered prior to the eventual translation of this diagnostic adjunct into the prenatal evaluation of these anomalies.

Concanavalin A binding of alpha-fetoprotein in amniotic fluid as an aid in the diagnosis of neural tube defects.

1981 ◽  
Vol 27 (10) ◽  
pp. 1658-1660 ◽  
Author(s):  
P K Buamah ◽  
P Taylor ◽  
A M Ward
Keyword(s):  
Spina Bifida ◽  
Amniotic Fluid ◽  
Neural Tube ◽  
Neural Tube Defects ◽  
Concanavalin A ◽  
Gestational Age ◽  
Alpha Fetoprotein ◽  
Normal Outcome ◽  
Fetal Malformations ◽  
Open Spina Bifida

Abstract Concanavalin A nonreactive alpha-fetoprotein was determined in samples of amniotic fluid from 16 abnormal pregnancies complicated by anencephaly (7), open spina bifida (6), intra-uterine death (1), anencephaly with exomphalos (1), or open spina bifida with exomphalos (1), and in amniotic fluid from 50 normal pregnancies with gestational age between 13 and 24 weeks. In all 16 cases with fetal malformations, the proportion of nonreactive alpha-fetoprotein was significantly decreased (median 5.3%) as compared with amniotic fluid from pregnancies with a normal outcome (median 39.7%). The results confirm that this measurement is useful in the diagnosis of neural tube defects, especially when the concentration of alpha-fetoprotein in amniotic fluid is normal or only slightly above normal and gestational age is uncertain.

The Amniotic Fluid As a Source of Neural Stem Cells in the Setting of Experimental Neural Tube Defects

2013 ◽  
Vol 22 (4) ◽  
pp. 548-553 ◽  
Author(s):  
Christopher G. Turner ◽  
Justin D. Klein ◽  
Junmei Wang ◽  
Devang Thakor ◽  
Darcy Benedict ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Amniotic Fluid ◽  
Neural Tube ◽  
Neural Tube Defects

scholarly journals Isolation of Human Neural Stem Cells from the Amniotic Fluid with Diagnosed Neural Tube Defects

2015 ◽  
Vol 24 (15) ◽  
pp. 1740-1750 ◽  
Author(s):  
Yu-Jen Chang ◽  
Hong-Lin Su ◽  
Lee-Feng Hsu ◽  
Po-Jui Huang ◽  
Tzu-Hao Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Amniotic Fluid ◽  
Neural Tube ◽  
Neural Tube Defects ◽  
Human Neural Stem Cells

Amniotic fluid concentrations of soluble endoglin and endothelial cell-specific molecule-1 in pregnancies complicated with neural tube defects

2020 ◽  
Vol 48 (2) ◽  
pp. 132-138
Author(s):  
Ali Ovayolu ◽  
Gamze Ovayolu ◽  
Tuncay Yuce ◽  
Murat Aykut Ozek ◽  
Ilkay Dogan ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Amniotic Fluid ◽  
Pregnant Women ◽  
Neural Tube ◽  
Neural Tube Defects ◽  
Gestational Age ◽  
Enzyme Linked Immunosorbent Assay ◽  
Study Group ◽  
Soluble Endoglin ◽  
Significant Difference

AbstractObjectiveTo determine the concentrations of soluble endoglin (sCD105) and endothelial cell-specific molecule-1 (ESM-1) in the amniotic fluid (AF) of pregnant women, and to investigate the relationship between these concentrations and neural tube defects (NTDs).MethodsAF concentrations of sCD105 and ESM-1 were measured in the study group, which included 60 pregnant women complicated with NTDs, and 64 pregnant women with unaffected healthy fetuses (control group). The AF concentrations of sCD105 and ESM-1 in both groups were measured using enzyme-linked immunosorbent assay and compared.ResultsThere were no significant differences in terms of the mean AF concentrations of sCD105 and ESM-1 between the groups (P=0.141, P=0.084, respectively). There was a significant difference between the AF sCD105 concentrations in those with gestational age <24 weeks (n=101) and ≥24 weeks (n=23) (X̅<24=76.35±126.62 vs. X≥24=39.87±58.32, P=0.041). AF ESM-1 concentrations were found to be statistically significant in the gestational age <22 weeks (n=90) and ≥22 weeks (n=34) groups (X̅<22=135.91±19.26 vs. X̅≥22=148.56±46.85, P=0.035). A positive and low-level relation at a statistically significant level was determined between the gestational age and AF ESM-1 concentration in the study group (r=0.257; P=0.048).ConclusionAF concentrations of sCD105 and ESM-1 were not associated with the development of NTDs. Unlike studies that reported that ESM-1 concentrations decreased in maternal plasma with increased gestational age, we determined an increase that was proportionate to gestational age in AF.

scholarly journals Neurone Specific Enolase in Amniotic Fluid: A Potential Marker of Anencephaly

1988 ◽  
Vol 25 (1) ◽  
pp. 85-88 ◽  
Author(s):  
K Spencer ◽  
P Carpenter
Keyword(s):  
Prenatal Diagnosis ◽  
Spina Bifida ◽  
Amniotic Fluid ◽  
Neural Tube ◽  
Neural Tube Defects ◽  
Normal Values ◽  
Potential Marker ◽  
Neuronal Proteins ◽  
Non Gaussian ◽  
Open Spina Bifida

Normal values for neurone specific enolase in amniotic fluid have been found to follow a non gaussian distribution with a 1–99 centile range of 1·10–4·32 μg/L. Neurone specific enolase levels have been shown to be raised in the amniotic fluid of pregnancies complicated by anencephaly, although not those complicated by open spina bifida. Neurone specific enolase measured by radioimmunoassay is capable of totally discriminating between normal pregnancies and those complicated by anencephaly. The study demonstrates the possible value of investigating other neuronal proteins which may find value as adjuncts to amniotic fluid Alpha fetoprotein levels in the prenatal diagnosis of Neural Tube Defects.

Impact of Gestational Age on Targeted Amniotic Cell Profiling in Experimental Neural Tube Defects

2014 ◽  
Vol 186 (2) ◽  
pp. 575 ◽  
Author(s):  
E.C. Pennington ◽  
K.L. Rialon ◽  
B. Dionigi ◽  
A. Ahmed ◽  
D. Zurakowski ◽  
...  
Keyword(s):  
Neural Tube ◽  
Neural Tube Defects ◽  
Gestational Age ◽  
Amniotic Cell

Detection of spina bifida in the first trimester of gestation in association with triploidy

2017 ◽  
Author(s):  
K.K. Otaryan , C.G. Gagaev
Keyword(s):  
Spina Bifida ◽  
Early Detection ◽  
Neural Tube ◽  
Neural Tube Defects ◽  
First Trimester ◽  
Termination Of Pregnancy ◽  
Diagnostic Tools ◽  
Prenatal Detection ◽  
Post Abortion

The case of prenatal detection of spina bifida at 12+3 weeks of gestation is described. Termination of pregnancy was performed at 13+3 weeks. Post-abortion karyotyping revealed triploidy (69XXX). Diagnostic tools for early detection of neural tube defects in the 1st trimester of gestation and subsequent appropriate management of pregnancy are discussed.

A Potential Systematic Error in Using Lysivane as Inhibitor in the Measurement of Amniotic Fluid Acetylcholinesterase by the Ellman Method

1983 ◽  
Vol 20 (3) ◽  
pp. 159-162 ◽  
Author(s):  
B A Webb ◽  
S J Richardson ◽  
R Garry ◽  
J Atkins
Keyword(s):  
Enzymatic Hydrolysis ◽  
Amniotic Fluid ◽  
Systematic Error ◽  
Neural Tube ◽  
Neural Tube Defects ◽  
Acidic Solution ◽  
Rate Of Increase ◽  
Background Absorption ◽  
Low Levels ◽  
Hydrolysis Of

The measurement of low levels of cholinesterase or acetylcholinesterase by the Ellman method requires correction for a non-enzymatic increase in absorption at 412 mμ that is due both to non-enzymatic hydrolysis of the acetylthiocholine substrate and to modification of the colour reagent. The rate of increase in absorption is dependent on temperature and pH. Addition of an acidic solution of lysivane to the assay solution for selective measurement of amniotic fluid acetylcholinesterase gives rise to a shift in pH; the use of methanol is suggested as an easier method of dissolving the inhibitor and does not affect the pH of the assay, obviating any need to redetermine the background absorption. There is, however, no improvement in ability of the method to predict pregnancies associated with neural-tube defects.

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