Chemically Soluble Fluoride in Na2FPO3/CaCO3-Based Toothpaste as an Indicator of Fluoride Bioavailability in Saliva during and after Toothbrushing

2020 ◽  
Vol 54 (2) ◽  
pp. 185-193
Author(s):  
Camila Siqueira Silva Coelho ◽  
Jaime Aparecido Cury ◽  
Cínthia Pereira Machado Tabchoury
Keyword(s):  
Fluoride Concentration ◽  
Area Under The Curve ◽  
Fluoride Ion ◽  
Selective Electrode ◽  
Fresh Sample ◽  
Group V ◽  
Group I ◽  
Adult Participants ◽  
The Relationship ◽  
Fluoride Ion Selective Electrode

The relationship between the concentration of chemically soluble fluoride found in toothpaste and that present in saliva, during and after brushing, was evaluated as an indicator of potentially bioavailable fluoride in toothpaste. Ten adult participants brushed their teeth with the assigned toothpastes: group I: fresh sample of a fluoride toothpaste: Na2FPO3/CaCO3, 1,378 µg F/g of total soluble fluoride (TSF); groups II–IV: aged samples of toothpaste presenting TSF concentrations of 1,160, 900, and 597 µg F/g, respectively; group V: non-F placebo toothpaste. The volunteers brushed their teeth for 1 min with 0.7 g of the toothpaste, all toothbrushing residues (TR) produced were collected, the mouth was rinsed with water, and saliva samples were collected up to 120 min. Total fluoride (TF) and TSF concentrations were determined in TR and in saliva samples using a fluoride ion-selective electrode. TSF concentration (µg F/mL) in TR was determined as an indicator of fluoride bioavailability during toothbrushing and the areas under curves of saliva fluoride concentration versus time (area under the curve, AUC = µg F/mL × min) were calculated as an indicator of fluoride bioavailability after toothbrushing. A significant correlation was found between the TSF concentrations in the toothpastes and the variables TR (r = 0.850; p = 0.0001) and AUC (r = 0.445; p = 0.004). For TF no significant correlation was found for TR (r = –0.099; p = 0.542) and AUC (r = –0.018; p = 0.912). The findings suggest that TSF concentration chemically found in Na2FPO3/CaCO3-based toothpaste could estimate how much fluoride would be bioavailable in saliva when the teeth are brushed.

Determination of inorganic fluoride in blood with a fluoride ion-selective electrode.

1987 ◽  
Vol 33 (2) ◽  
pp. 253-255 ◽  
Author(s):  
E Kissa
Keyword(s):  
Whole Blood ◽  
Fluoride Concentration ◽  
Ion Selective Electrode ◽  
Fluoride Ion ◽  
Inorganic Fluoride ◽  
Selective Electrode ◽  
Cell Potential ◽  
Novel Method ◽  
Fluoride Ion Selective Electrode

Abstract Inorganic fluoride in whole blood, serum, or plasma has been determined with a fluoride ion-selective electrode by adding the 0.5- to 2.0-mL sample to 20 mL of water containing a buffer and fluoride, 25 micrograms/L. The fluoride concentration in the sample is calculated from the resulting cell potential difference recorded after equilibrating for 10 min. This novel method has the advantages of simplicity, accuracy, and high precision, standard deviations for 5 to 7 replicate determinations of fluoride ranging from +/- 1.7 to +/- 2.4 micrograms/L. For whole blood from donors living in an area with fluorinated drinking water, the F- concentration was 20 to 60 micrograms/L.

scholarly journals Analysis of fluoride concentration in commercial bottled waters

2015 ◽  
Vol 63 (4) ◽  
pp. 461-466
Author(s):  
Davi Oliveira BIZERRIL ◽  
Janaína Rocha de Sousa ALMEIDA ◽  
Kátia de Góis Holanda SALDANHA ◽  
Ronaldo Emilio CABRAL FILHO ◽  
Maria Eneide Leitão de ALMEIDA
Keyword(s):  
Fluoride Concentration ◽  
Health Surveillance ◽  
Bottled Water ◽  
Fluoride Ion ◽  
Careful Monitoring ◽  
Selective Electrode ◽  
Cross Sectional ◽  
The City ◽  
Fluoride Ion Selective Electrode ◽  
Concentration Levels

Objective: Evaluate fluoride concentration in 500ml commercial brands of bottled water and compare it to the amount printed on the label. Methods: A descriptive, cross-sectional epidemiological study was conducted. Samples of nine different commercial brands of 500ml bottled water were collected at authorized distribution points in the city of Fortaleza, CE, Brazil, in 2013. Fluoride concentration was determined in duplicate using a fluoride ion-selective electrode. The results were obtained from linear regression of the calibration curve. Results: Fluoride concentrations ranged between 0.07 and 0.63 ppm F- or mg F/L. There were significant differences between the values printed on the labels and those found in the analysis. The fluoride concentration levels determined were not in compliance with the recommended levels by the National Health Surveillance Agency. Conclusions: The results obtained confirm the need for more careful monitoring of fluoride levels in bottled water and closer sanitary inspections.

Soluble fluoride in Na2FPO3/CaCO3-based toothpaste as an indicator of systemically bioavailable fluoride

2021 ◽  
Author(s):  
Deborah Rackel Caldas da Rocha ◽  
Antonio Pedro Ricomini Filho ◽  
Jaime Aparecido Cury
Keyword(s):  
Body Weight ◽  
Blood Plasma ◽  
Fluoride Concentration ◽  
Fluoride Ion ◽  
Fresh Sample ◽  
Treatment Groups ◽  
Statistical Correlations ◽  
Group I ◽  
Total Urine ◽  
In Blood Plasma

Fluoride chemically soluble in toothpaste is an indicator of fluoride bioavailability when the teeth are brushed and the same should be expected systemically when toothpaste is ingested. A four-phases study was conducted, in which eight participants were subjected in each phase to one of the assigned treatment groups: Group I: fresh sample of a Na2FPO3/CaCO3 toothpaste with 1,334 μg F/g of total soluble fluoride (TSF); groups II–IV: aged samples of toothpaste presenting TSF concentrations of 1,128, 808, and 687 μg F/g, respectively. In all phases, the participants ingested an amount of toothpaste equivalent to 70.0 µg F/kg body weight, as total fluoride (TF). Blood was collected before (baseline) and up to 180 min after toothpaste ingestion as indicator of fluoride bioavailability. Total urine (24 h before and 24 h after ingestion) was collected as indicator of absorbed fluoride that was excreted. F concentration in blood plasma and urine was determined with a fluoride ion-specific electrode. The areas under the curve of F concentration vs. time (AUC=ng F/ml x min) and the peaks of fluoride concentration in blood plasma (Cmax) were calculated. The net amount of fluoride excreted (mg/day) was calculated by subtraction. A significant correlation of the amount (mg) of TSF ingested was found between the AUC (r= 0.76; p<0.01) and Cmax (r= 0.86; p<0.01) in plasma, and the fluoride excreted (r= 0.65; p<0.01). For TF no statistical correlations were found (p>0.05). Data suggest that the concentration of TSF found in Na2FPO3/CaCO3-based toothpastes is a useful predictor of how much fluoride will be systemically bioavailable when this type of formulation is ingested.

scholarly journals Analysis of Fluoride Content in Alternative Milk Beverages

2019 ◽  
Vol 43 (6) ◽  
pp. 388-392 ◽  
Author(s):  
Janice A Townsend ◽  
Tatyana Thompson ◽  
Skylar Vaughn ◽  
Yapin Wang ◽  
Qingzhao Yu ◽  
...  
Keyword(s):  
Fluoride Concentration ◽  
Bovine Milk ◽  
Fluoride Ion ◽  
Soy Milk ◽  
Selective Electrode ◽  
Nutrition Labels ◽  
Whole Milk ◽  
Different Types ◽  
Fluoride Ion Selective Electrode ◽  
Mean Concentration

Background: The dentist has a responsibility to provide nutritional counseling and fluoride consumption recommendations. The purpose of this study was to measure and compare the concentrations of fluoride in a large number of alternative milk beverages and bovine milk. Study design: Thirty-three milk alternatives, including 9 diverse types and 11 different brands, were analyzed using a fluoride ion-selective electrode (ISE) and an ISE meter. Fluoride concentrations were then compared among different types and between different brands. Results: Fluoride concentration ranged from 0.01 ppm (Malk® Pure Cashew Milk) to 0.80 ppm (Almond Breeze® Original Unsweetened Almond Milk) with a mean concentration of 0.32 ppm. When compared, bovine whole milk (0.03±0.00 ppm) was found to be significantly lower in fluoride than all samples analyzed except Malk Pure Cashew Milk, Soy Milk Vanilla, Rice Milk, and Pecan Milk. Major differences also existed between the same milk alternative types of different brands. Conclusion: The amount of fluoride varies among different types of milk alternatives and different brands. To ensure that the dental team can provide proper recommendations regarding fluoride use, manufacturers should consider placing fluoride concentrations on nutrition labels.

scholarly journals simplified protocol to determine total fluoride concentration in NaF/ silica-based toothpastes

2020 ◽  
Vol 19 ◽  
pp. e201689
Author(s):  
Jenniffer Quiroz-Torres ◽  
Cinthia Pereira Machado Tabchoury ◽  
Carlos Liñán-Durán ◽  
Antonio Pedro Ricomini-Filho ◽  
Ailin Cabrera-Matta ◽  
...  
Keyword(s):  
Fluoride Concentration ◽  
T Test ◽  
Ion Selective Electrode ◽  
Fluoride Ion ◽  
Selective Electrode ◽  
Direct Technique ◽  
The Mean ◽  
Fluoride Salts ◽  
Fluoride Ion Selective Electrode

Aim: To determine total fluoride (TF) concentration in Na2FPO3/Ca-based toothpastes, using fluoride ion selective electrode (F-ISE) by the direct technique, it is necessary to use acid (Ac+) to hydrolyze the FPO3 2- ion and to dissolve insoluble fluoride salts bound to the abrasive. For NaF/silicabased toothpastes, the use of acid is not necessary (Ac-) and a simplified protocol could be followed. Methods: Thus, we evaluated TF concentration in seven brands of NaF/silicabased toothpastes, following the validated conventional Cury’s protocol (Ac+) or a simplification of this protocol (Ac-). Fluoride was analyzed with ISE calibrated with fluoride standard solutions prepared in the same conditions as the samples (Ac+ or Ac-). Results: The mean (±SD; n=21) of TF concentrations (μg F/g) found by Ac+ (971.3±191.2) and Ac- (982.4±201.3) protocols were not statistically different (t test, p=0.22). The TF concentrations found agree with those declared by the manufacturers, except for one toothpaste imported from China. Conclusion: The findings suggest that the determination of fluoride in NaF/silica-based toothpastes can be accurately made using a simplified protocol of analysis.

Automated Fluoride Ion Determination

1977 ◽  
Vol 14 (1-6) ◽  
pp. 269-274 ◽  
Author(s):  
David C. Cowell
Keyword(s):  
Continuous Flow ◽  
Ion Concentration ◽  
Fluoride Ion ◽  
Selective Electrode ◽  
Automated Method ◽  
Electrode Response ◽  
Kinetics Of ◽  
Fluoride Ion Selective Electrode ◽  
Flow Techniques

An automated method is described, using standard continuous flow techniques, for the determination of urine fluoride ion concentration using a fluoride ion selective electrode. It is shown that the kinetics of the electrode response to changes in fluoride ion can be used for the accurate measurement of fluoride ion concentration in urine, and that equilibration of the electrode response is not a prerequisite for the measurement of fluoride ion. Recovery experiments are in the range 83 to 90%; in-batch precision is between 0·9 and 1·6% and carryover 2·5% or less.

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