Spatial Connections between Microplastics and Heavy Metal Pollution within Floodplain Soils

Soils contain an increasing number of different pollutants, which are often released into the environment by human activity. Among the “new” potential pollutants are plastics and microplastics. “Recognized” pollutants such as heavy metals, of geogenic and anthropogenic origin, now meet purely anthropogenic contaminants such as plastic particles. Those can meet especially in floodplain landscapes and floodplain soils, because of their function as a temporary sink for sediments, nutrients, and pollutants. Based on a geospatial sampling approach, we analyzed the soil properties and heavy metal contents (ICP-MS) in soil material and macroplastic particles, and calculated total plastic concentrations (Ptot) from preliminary studies. Those data were used to investigate spatial connections between both groups of pollutants. Our results from the example of the Lahn river catchment show a low-to-moderate contamination of the floodplain soils with heavy metals and a wide distribution of plastic contents up to a depth of two meters. Furthermore, we were able to document heavy metal contents in macroplastic particles. Spatial and statistical correlations between both pollutants were found. Those correlations are mainly expressed by a comparable variability in concentrations across the catchment and in a common accumulation in topsoil and upper soil or sediment layers (0–50 cm). The results indicate comparable deposition conditions of both pollutants in the floodplain system.

Static and Dynamic Statistical Correlations in Water: Comparison of Classical Ab Initio Molecular Dynamics at Elevated Temperature With Path Integral Simulations at Ambient Temperature

It is a common practice in ab initio molecular dynamics (AIMD) simulations of water to use an elevated temperature to overcome the over-structuring and slow diffusion predicted by most current density functional theory (DFT) models. The simulation results obtained in this distinct thermodynamic state are then compared with experimental data at ambient temperature based on the rationale that a higher temperature effectively recovers nuclear quantum effects (NQEs) that are missing in the classical AIMD simulations. In this work, we systematically examine the foundation of this assumption for several DFT models as well as for the many-body MB-pol model. We find for the cases studied that a higher temperature does not correctly mimic NQEs at room temperature, which is especially manifest in significantly different three-molecule correlations as well as hydrogen bond dynamics. In many of these cases, the effects of NQEs are the opposite of the effects of carrying out the simulations at an elevated temperature.

Ultrafast Doppler imaging and Ultrasound Localization Microscopy reveal the complexity of vascular rearrangement in chronic spinal lesion

Acute spinal cord injury (SCI) leads to severe damage to the microvascular network. The process of spontaneous repair is accompanied by formation of new blood vessels; their functionality, however, presumably very important for functional recovery, has never been clearly established, as most studies so far used fixed tissues. Here, combining ultrafast Doppler imaging and Ultrasound Localization Microscopy (ULM) on the same animals, we proceeded at a detailed analysis of structural and functional vascular alterations associated with the establishment of chronic SCI, both at macroscopic and microscopic scales. Using a standardized animal model of SCI, our results demonstrate striking hemodynamic alterations in several subparts of the spinal cord: a reduced blood velocity in the lesion site, and an asymmetrical hypoperfusion caudal but not rostral to the lesion. In addition, the worsening of many evaluated parameters at later time points suggests that the neoformed vascular network is not yet fully operational, and reveals ULM as an efficient in vivo readout for spinal cord vascular alterations. Finally, we show statistical correlations between the diverse biomarkers of vascular dysfunction and SCI severity. The imaging modality developed here will allow evaluating recovery of vascular function over time in pre-clinical models of SCI. Also, used on SCI patients in combination with other quantitative markers of neural tissue damage, it may help classifying lesion severity and predict possible treatment outcomes in patients.

A Quantitative Study on Statistical Associations between Extroversion-Introversion Personality Traits and Anxiety in Different Stages of Lockdown during the Covid-19 Pandemic

The Covid-19 pandemic has caused several changes in the human state of mind, in particular adapting to the culture of the new normal while lockdown measures are implemented. This study explored the effect of the lockdown measure on the level of anxiety of high school students, comparing those identified as introverts and extroverts. Participants (N = 103) filled out the given survey, which determined that they were both introverts or extroverts and the level of anxiety that they had before, during, and after the lockdown caused by the pandemic. According to statistical analysis, the result showed that the level of anxiety perceived by those feeling the sense of extroversion was statistically higher than those with introversion, at the significance level of 95%. In addition, the analysis revealed that there was no correlation between extroverts and anxiety before, during, and after the lockdown measures. On the other hand, there were statistical correlations between the level of introversion and the level of anxiety in every stage of lockdown: before, during and after, indicating that the lockdowns due to the global pandemic did not affect extroverted people anxiety as much as it affected introverts. Moreover, it also showed that the level of anxiety of the introverts has become even more intensified even after the lockdown.

The Impact of Mosaic Land Use and Land Cover on the Quality of River Waters (Case Study: Lubelskie Province, E Poland)

Human activity with respect to land use and land cover can impact the quality of river water. Understanding the correlation between these two factors makes it possible to improve the management of space and mitigate negative human impacts on the environment. The present study encompassed 34 catchments in Lubelskie Province, a typically agricultural region in eastern Poland. Six main types of land use and land cover (LULC) and 15 indices of water quality have been analysed. An unequivocally negative impact of LULC on the river water parameters has not been found. For some indices, no correlations occur, while for others the statistical correlations are weak. One possible reason is the impact of other factors or the unique land use mosaic of the province having a significant impact on the circulation of pollutants in the landscape. It may be that the absence of clear correlations is the result of the relatively low anthropogenic pressure in the region.

Soluble fluoride in Na2FPO3/CaCO3-based toothpaste as an indicator of systemically bioavailable fluoride

Fluoride chemically soluble in toothpaste is an indicator of fluoride bioavailability when the teeth are brushed and the same should be expected systemically when toothpaste is ingested. A four-phases study was conducted, in which eight participants were subjected in each phase to one of the assigned treatment groups: Group I: fresh sample of a Na2FPO3/CaCO3 toothpaste with 1,334 μg F/g of total soluble fluoride (TSF); groups II–IV: aged samples of toothpaste presenting TSF concentrations of 1,128, 808, and 687 μg F/g, respectively. In all phases, the participants ingested an amount of toothpaste equivalent to 70.0 µg F/kg body weight, as total fluoride (TF). Blood was collected before (baseline) and up to 180 min after toothpaste ingestion as indicator of fluoride bioavailability. Total urine (24 h before and 24 h after ingestion) was collected as indicator of absorbed fluoride that was excreted. F concentration in blood plasma and urine was determined with a fluoride ion-specific electrode. The areas under the curve of F concentration vs. time (AUC=ng F/ml x min) and the peaks of fluoride concentration in blood plasma (Cmax) were calculated. The net amount of fluoride excreted (mg/day) was calculated by subtraction. A significant correlation of the amount (mg) of TSF ingested was found between the AUC (r= 0.76; p<0.01) and Cmax (r= 0.86; p<0.01) in plasma, and the fluoride excreted (r= 0.65; p<0.01). For TF no statistical correlations were found (p>0.05). Data suggest that the concentration of TSF found in Na2FPO3/CaCO3-based toothpastes is a useful predictor of how much fluoride will be systemically bioavailable when this type of formulation is ingested.

Translocating lipopolysaccharide correlates with the severity of enterovirus A71-induced HFMD by promoting pro-inflammation and viral IRES activity

Background The increase of inflammation-inducing enterobacteria was recently observed in severe hand, foot, and mouth disease (HFMD) caused by Enterovirus A71 (EV-A71). This study aimed to verify the occurrence of bacterial translocation (BT) and further explore the contributory role of BT to severity of EV-A71-mediated HFMD cases. Methods Serum specimens from 65 mild and 65 severe EV-A71-associated HFMD cases and 65 healthy children were collected. EV-A71 VP1 in serum, inflammatory mediators including C-reactive protein, IL-1β, IL-6, interferon-γ and tumor necrosis factor-α, BT related biomarkers including Claudin-3, intestinal fatty acid binding protein, lipopolysaccharide (LPS), soluble CD14 (sCD14) and endotoxin core antibody were measured by ELISA. Bacterial DNA (BactDNA) fragments were quantified by quantified PCR (qPCR). Rhabdomyosarcoma (RD) or SH-SY5Y cells, infected with LPS-pre-incubated EV-A71 or transfected with plasmid containing viral 2Apro or mRNA containing viral internal ribosomal entry site (IRES), were post-treated with or without LPS in vitro. EV-A71 RNA and viral or cellular proteins were determined by qPCR and western blot, respectively. Results Compared to mild HFMD patients, remarkably higher inflammatory mediators as well as BT-related biomarkers except BactDNA were observed in severe HFMD cases (all P < 0.05). In severe HFMD group, circulating concentrations of LPS and sCD14 showed statistical correlations with inflammation indices (all P < 0.05), serum levels of EV-A71 VP1 were found to be positively correlated with serum LPS (r = 0.341, P = 0.005) and serum sCD14 (r = 0.458, P < 0.001). In vitro, EV-A71 attachment and internalization were only slightly promoted by LPS pre-incubation; however, EV-A71 proliferation and viral 2Apro-mediated IRES activity were significantly accelerated by LPS post-treatment. Conclusions Our results collectively indicate that gut-derived translocating LPS contributes to the severity of EV-A71-induced HFMD by driving inflammatory response and viral proliferation via viral 2Apro-mediated IRES.

Self-Determination Theory: Statistical Correlations Between Motivational Regulations and Basic Psychological Needs

Self-determination theory has been predominantly used as a psychological framework to describe human motivation. It classifies three types of motivation with various forms of regulations, ranging from amotivation, extrinsic motivation (i.e., extrinsic regulation, introjected regulation, identified regulation, and integrated regulation), to intrinsic motivation. In addition, it points out three basic psychological needs (i.e., autonomy, relatedness, competence) which are essential components to enhance the process of internalisation, meaning motivations driven by identified, integrated and intrinsic regulations. This study aims to provide statistical evidence of statistical correlations among these factors and regulations. To achieve this, an online questionnaire with 9 close-ended statements based on 5-Likert scale was distributed to high-school students and school leavers in Thailand. A total of 206 responses were received. Overall, a correlational analysis showed a positive correlation between internalisation and the basic psychological needs (r = 0.4). Furthermore, there was a moderate positive correlation between integrated correlation and intrinsic motivation (r = 0.6) which support the described theoretical framework. In addition, there was a moderate positive correlation between autonomy and competence (r = 0.6) in the perspective of psychological needs. However, the statistical evidence shows a moderate relationship between identified regulation and external regulation (r = 0.6) which adds an insightful finding to our current understanding. It is suggested that in order to increase the level of internalisation, it is essential to provide a learning environment where students can feel the sense of success (competence), constructive relationship with others (relatedness), and a fair degree of self-regulated learning (autonomy).

Ultrafast Doppler imaging and Ultrasound Localization Microscopy reveal the complexity of vascular rearrangement in chronic spinal lesion

Acute spinal cord injury (SCI) leads to severe damage to the microvascular network. The process of spontaneous repair is accompanied by formation of new blood vessels; their functionality, however, presumably very important for functional recovery, has never been clearly established, as most studies so far used fixed tissues. Here, combining ultrafast Doppler imaging and Ultrasound Localization Microscopy (ULM) on the same animals, we proceeded at a detailed analysis of structural and functional vascular alterations associated with the establishment of chronic SCI, both at macroscopic and microscopic scales. Using a standardized animal model of SCI, our results demonstrate striking hemodynamic alterations in several subparts of the spinal cord: a reduced blood velocity in the lesion site, and an asymmetrical hypoperfusion caudal but not rostral to the lesion. In addition, the worsening of many evaluated parameters at later time points suggests that the neoformed vascular network is not yet fully operational, and reveals ULM as an efficient in vivo readout for spinal cord vascular alterations. Finally, we show statistical correlations between the diverse biomarkers of vascular dysfunction and SCI severity. The imaging modality developed here will allow evaluating recovery of vascular function over time in pre-clinical models of SCI. Also, used on SCI patients in combination with other quantitative markers of neural tissue damage, it may help classifying lesion severity and predict possible treatment outcomes in patients.