scholarly journals Application of Saliva Inhibition to Detect Underlying Alloantibodies in Bombay Blood Group

2021 ◽  
pp. 1-5
Author(s):  
Lingbo Wang ◽  
Michael Crennan ◽  
Angela Benic ◽  
Derek Chiu ◽  
Fiona Morris ◽  
...  
Keyword(s):  
Blood Group ◽  
Autologous Blood ◽  
Transfusion Practice ◽  
Abo Blood Group ◽  
Plasma Samples ◽  
Case Presentation ◽  
Secretor Status ◽  
Thermal Amplitude ◽  
Novel Method ◽  
Clinically Significant

<b><i>Introduction:</i></b> The Bombay phenotype is a rare blood group determined by the absence of H antigens. Bombay individuals produce anti-H, a clinically significant antibody that react against all ABO blood group. Anti-H can mask underlying alloantibody during antibody investigation, a challenge in current transfusion practice. The aim of this article is to explore saliva inhibition, a novel method to detect underlying alloantibody in Bombay individuals. <b><i>Case Presentation:</i></b> The case is a 93-year-old female transfused with pre-donated autologous blood for a surgery. We determined anti-H subclass and thermal amplitude, secretor status, and optimal ratio of saliva and Bombay plasma. Plasma samples containing anti-H were spiked with anti-Fy(a) to determine the effectiveness of saliva inhibition in uncovering underlying alloantibodies. <b><i>Results:</i></b> Anti-H was confirmed to be predominately IgM with broad thermal amplitude. Tube immediate spin (IS) showed stronger anti-H reactivity compared to column agglutination technology (CAT). Spiked anti-Fy(a) was successfully detected using saliva inhibition method. <b><i>Conclusion:</i></b> Tube IS appears more sensitive to anti-H. Saliva inhibition appears to be a promising method to detect underlying alloantibody in the plasma of Bombay phenotype individuals.

scholarly journals ABO blood group, secretor status and detection ofHelicobacter pyloriamong patients with gastric or duodenal ulcers

1991 ◽  
Vol 106 (2) ◽  
pp. 221-229 ◽  
Author(s):  
A. Mentis ◽  
C. C. Blackwell ◽  
D. M. Weir ◽  
C. Spiliadis ◽  
A. Dailianas ◽  
...  
Keyword(s):  
Duodenal Ulcer ◽  
Helicobacter Pylori ◽  
Gastric Ulcer ◽  
Blood Group ◽  
Local Population ◽  
Abo Blood Group ◽  
Duodenal Ulcers ◽  
Secretor Status ◽  
Gastric Or Duodenal Ulcers ◽  
H Pylori

SUMMARYPatients (454) referred for gastroscopy to the General Hospital of Athens were examined to determine (1) if non-secretors were over-represented among patients with ulcers and (2) is there was an association with ABO blood group or secretor status and carriage ofHelicobacter pylori.Compared with the local population, among patients with either gastric ulcer (51) or duodenal ulcer (96) there was a significant increase in the proportion of those who were blood group O (P< 0·025); however, there were no significant differences in the proportions of non-secretors.H. pyloriwas identified in 62 % of the 454 patients: 59·5 % of those without evidence of ulcers; 62·5 % of those with gastric ulcer; 88% of those with duodenal ulcer (P< 0·0005). These bacteria were cultured more often and in higher numbers from patients with duodenal ulcer (P< 0·025). There was no association between ABO blood group and prevalence ofH. pylori. The prevalence ofH. pyloriamong non-secretors with gastric ulcer (12·5%) was significantly lower than that for non-secretors with duodenal ulcer (100%) (P< 0·0005). This was not observed for secretors.

scholarly journals Recurrent bacteriuria and primary biliary cirrhosis: ABO blood group, P1 blood group, and secretor status.

1984 ◽  
Vol 37 (9) ◽  
pp. 1055-1058 ◽  
Author(s):  
I J Rosenstein ◽  
G R Hazlehurst ◽  
A K Burroughs ◽  
O Epstein ◽  
S Sherlock ◽  
...  
Keyword(s):  
Primary Biliary Cirrhosis ◽  
Blood Group ◽  
Abo Blood Group ◽  
Biliary Cirrhosis ◽  
Secretor Status

Normal Human Serum Contains Natural Antibodies Reactive With Autologous ABO Blood Group Antigens

Blood ◽  
1999 ◽  
Vol 93 (12) ◽  
pp. 4418-4424 ◽  
Author(s):  
Sergio H. Spalter ◽  
Srini V. Kaveri ◽  
Emmanuelle Bonnin ◽  
Jean-Claude Mani ◽  
Jean-Pierre Cartron ◽  
...  
Keyword(s):  
Human Serum ◽  
Blood Group ◽  
Autologous Blood ◽  
Natural Antibodies ◽  
Normal Human Serum ◽  
Abo Blood Group ◽  
Blood Group Antigens ◽  
Blood Group A ◽  
Group A ◽  
Normal Human

Abstract It is widely accepted that the serum of healthy individuals contains natural antibodies only against those blood group A or B antigens that are not expressed on the individual’s red blood cells. The mechanisms involved in tolerance to autologous blood group antigens remain unclear. In the present study, we show that IgM and IgG antibodies reactive with autologous blood group antigens are present in the immunoglobulin fraction of normal human serum. Natural IgG anti-A antibodies purified by affinity chromatography from IgG of individuals of blood group A exhibited an affinity for A trisaccharide antigen in the micromolar range and agglutinated A red cells at sixfold higher concentrations than those required for agglutination with affinity-purified anti-A IgG of individuals of blood group B. Whereas autoantibodies reactive with self A and B antigens are readily detected in purified IgG and IgM fractions, their expression is restricted in whole serum as a result of complementary interactions between variable regions of antibodies. These observations suggest that tolerance to autologous ABO blood group antigens is dependent on peripheral control of antibody autoreactivity.

scholarly journals ABO blood group system and bone marrow transplantation

Blood ◽  
1977 ◽  
Vol 50 (2) ◽  
pp. 185-194 ◽  
Author(s):  
RP Gale ◽  
S Feig ◽  
W Ho ◽  
P Falk ◽  
C Rippee ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Transplantation ◽  
Blood Group ◽  
Marrow Transplantation ◽  
Abo Blood Group ◽  
Blood Group System ◽  
Group System ◽  
Antibody Absorption ◽  
Clinically Significant

Abstract The role of the ABO blood group system in determining the outcome of bone marrow transplantation was investigated in 53 patients with aplastic anemia and acute leukemia grafted from HLA-identical siblings. There was no correlation between ABO compatibility and marrow engraftment, graft rejection, or graft-versus-host disease. In 5 recipients with antibodies prior to transplantation to antigens of the ABH system present on the cells of their donors, plasma exchange and antibody absorption in vivo were effective in permitting engraftment of ABO-incompatible bone marrow. These findings indicate that the ABO system is not a clinically significant barrier to successful bone marrow transplantation in otherwise histocompatible individuals.

scholarly journals ABO Blood Group in Patients with Peptic Ulcer Disease: Association with Secretor Status

2016 ◽  
Vol 9 (3) ◽  
Author(s):  
Muhammad Naveed Akhtar ◽  
Auhammad Tayyib ◽  
Tahira Tasneem ◽  
Anjum Rashid Butt
Keyword(s):  
Peptic Ulcer ◽  
Peptic Ulcer Disease ◽  
Blood Group ◽  
Abo Blood Group ◽  
Direct Agglutination Test ◽  
Ulcer Disease ◽  
Secretor Status ◽  
Abo Blood Grouping ◽  
Group Association ◽  
Blood Grouping

Aims and objective of the study is to find ABO blood group association with secretor status in peptic ulcer disease patients. ABO blood grouping by direct agglutination test and secretor status by agglutination inhibition test. 50 controls and 50 endoscopically diagnosed PUD patients were selected. Blood group O is associated with duodenal ulcer but no association with secretor status was found.

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