scholarly journals EFFECTS OF MELATONIN ON OXIDANT AND ANTIOXIDANT STATUSIN THE BLOOD OF ALLOXAN DIABETIC RATS

2019 ◽  
pp. 5-10
Author(s):  
N. M. Luhinich ◽  
I. V. Gerush ◽  
N. P. Grygorieva
Keyword(s):  
Antioxidant Enzymes ◽  
Free Radical ◽  
Free Radical Oxidation ◽  
Diabetic Rats ◽  
Sod Activity ◽  
Radical Oxidation ◽  
Modified Proteins ◽  
Oxidatively Modified ◽  
Ceruloplasmin Activity ◽  
Oxidatively Modified Proteins

Introduction. The experimental model of alloxane diabetes is quite common, which is often used to study the different aspects of pathogenesis and pathomorphology of diabetes. It is known that during the diabetes activation of free radical oxidation of biomolecules occurs as well as depletion of the antioxidant system. Melatonin can suppress reactive oxygen species and increases the activity of antioxidant enzymes. The aim of the study – to investigate the effect of melatonin on oxidant and antioxidant status in the blood of alloxan diabetic rats. Research Methods. Experiments were conducted on white outbred sexually mature male rats. In the blood plasma was determined content of oxidatively modified proteins, the ceruloplasmin activity; in the hemolisate of erythrocytes was determined content of TBA-reactive products, the measurement of superoxide dismutase (SOD) and catalase activities. Results and Discussion. The results of our studies showed that under conditions of alloxane diabetes, processes of free radical damage to biomolecules are intensified, as evidenced by the increase in the content of TBA-active products and oxidatively modified proteins in the blood of rats on 7 and 14 days of alloxane diabetes. The activity of catalase and ceruloplasmin in the blood of alloxan diabetic rats was lower than in the control group of animals but the SOD activity was significantly increased. The positive effect of melatonin is shown on 7 and 14 days and decrease the content of TBA-active products and oxidatively modified proteins blood of rats compared with untreated animals. Also, the administration of melatonin contributed to the normalization of the activity antioxidant enzymes of blood in rats with alloxane diabetes: increase of catalase activity in erythrocytes, ceruloplasmin activity and decrease SOD activity compared with untreated animals. Conclusions. The introduction of exogenous melatonin in rats with alloxane diabetes in a dose of 10 mg/kg daily for 7 and 14 days cause a pronounced antioxidant effect, reducing free radical oxidation and normalizing the activity of enzymes of antioxidant defense in the blood alloxan diabetic rats.

scholarly journals Intensity of free radical processes in cartilage of rats with collagen-induced arthritis

2016 ◽  
Vol 21 (2) ◽  
pp. 27-30
Author(s):  
E. Tihova ◽  
O. Bernyk ◽  
O. Savchuk ◽  
K. Dvorshchenko ◽  
L. Ostapchenko
Keyword(s):  
Superoxide Dismutase ◽  
Antioxidant Enzymes ◽  
Free Radical ◽  
Experimental Model ◽  
Superoxide Anion ◽  
Anion Radical ◽  
Free Radical Oxidation ◽  
Collagen Induced Arthritis ◽  
Radical Oxidation ◽  
Radical Processes

Products of free radical oxidation and antioxidant enzymes in rats with collagen-induced arthritis were studied. It was established, that under experimental model of arthritis in rat cartilage the content of superoxide anion radical, peroxide and TBA-active compounds and the activity of antiradical enzymes superoxide dismutase and catalase increased.

EFFECT OF A TRANSDERMAL FILM WITH ERYTHROPOIETIN ON LYMPHOCYTE DEATH AND FREE RADICAL OXIDATION IN BLOOD OF RATS WITH EXPERIMENTAL THERMAL INJURY

2019 ◽  
pp. 72-79
Author(s):  
М.В. Осиков ◽  
Е.В. Симонян ◽  
О.Т. Башарова
Keyword(s):  
Free Radical ◽  
Catalase Activity ◽  
Thermal Injury ◽  
Free Radical Oxidation ◽  
Sod Activity ◽  
Radical Oxidation ◽  
Early Apoptosis ◽  
Late Apoptosis ◽  
Transdermal Film ◽  
Plasma Activity

Цель - изучение влияния эритропоэтина в составе трансдермальной пленки на характер гибели лимфоцитов и процессы свободнорадикального окисления в крови при экспериментальной термической травме у крыс. Методика. Эксперименты выполнены на 90 белых нелинейных крысах-самцах массой 220±20 г. Животные были случайным образом разделены на 3 группы: группа 1 (n=10) - интактный контроль, группа 2 (n=40) - термическая травма с наложением на область ожога асептической повязки, группа 3 (n=40) - термическая травма с наложением на область ожога трансдермальной пленки с эритропоэтином. Термическую травму ІІІА степени (площадь 3,5% поверхности) воспроизводили под общим наркозом погружением межлопаточной области кожи в очищенную воду (t= 98-99 ºС). Эритропоэтин (42 МЕ/см2) в составе трансдермальной пленки (площадь 12 см2) наносили ежедневно сразу после травмы. Исследование крови проводили на 3-и, 5-е, 8-е и 14-е сут. Гибель лимфоцитов, выделенных из крови на градиенте плотности фиколл-верографин (1,077), оценивали при окрашивании клеток конъюгированным с флюорохромом аннексином V и 7-аминоактиномицином D. Дифференцировали интактные клетки, клетки с ранними признаками апоптоза, клетки с поздними признаками апоптоза и частично некротизированные клетки. Содержание продуктов ПОЛ оценивали спектрофотометрически, определяли активность супероксиддисмутазы в плазме, каталазы в плазме крови и эритроцитах. Результаты. Показано, что при термической травме увеличивается количество лимфоцитов в крови с ранними и поздними признаками апоптоза и некроза, содержание диеновых конъюгатов, кетодиенов и сопряженных триенов, оснований Шиффа в липидных экстрактах плазмы и лимфоцитов, возрастает также активность каталазы в плазме и эритроцитах, активность супероксиддисмутазы в плазме крови снижается. В условиях применения эритропоэтина в составе трансдермальной пленки наблюдается уменьшение количества лимфоцитов с ранними и поздними признаками гибели, снижается активность процессов ПОЛ в плазме и лимфоцитах, а также активность каталазы в плазме и эритроцитах и возрастает активность супероксиддисмутазы в плазме крови. Заключение. Использование эритропоэтина в составе трансдермальной пленки при термической травме снижает выраженность гибели лимфоцитов, активность процессов ПОЛ, повышает активность супероксиддисмутазы в плазме крови по сравнению с группой животных с термической травмой без применения эритропоэтина. Aim. Studying the effect of erythropoietin (EPO) as a component of the transdermal film (TDF) on lymphocyte apoptotic death and free radical oxidation in blood of rats with experimental thermal injury (TI) (trauma). Methods. Experiments were performed on 90 white mongrel male rats weighing 220±20 g. The IIIA grade TI with a burn area of 3.5% body surface was induced under general anesthesia by immersing the interscapular region of the skin in purified water at 98-99ºС. EPO (42 IU/cm2) as a component of the 12 cm2 TDF was applied daily, immediately after the TI. The animals were randomized to three groups: group 1 (n=10), intact control; group 2 (n=40), TI with application of an aseptic dressing to the burn area; group 3 (n=40), TI with application of the EPO-containing TDF to the burn area. The study was conducted at days 3, 5, 8, and 14 after TI. Lymphocytes were isolated from blood using the ficoll-verographin density gradient (1.077) and their apoptosis was evaluated by staining the cells with fluorochrome-conjugated Annexin V (Annexin-5-FITC) and 7-aminoactinomycin D (7-AAD). The cells were differentiated into intact cells, cells with early apoptosis signs, cells with late apoptosis signs, and partially necrotized cells. Concentration of lipid peroxidation (LP) products was measured spectrophotometrically; superoxide dismutase (SOD) activity was measured in plasma and catalase activity was measured in plasma and red blood cells. Results. TI increased the count of lymphocytes of the Annexin-5-FITC+/7-AAD (early apoptosis signs) and Annexin-5-FITC+/7-AAD+ (late apoptosis or necrosis signs) phenotypes, and concentrations of dienic conjugates, ketodienes, conjugated trienes, and Schiff bases in heptanol and isopropanol phases of plasma and lymphocyte lipid extracts. Plasma and red cell catalase activities were increased whereas plasma SOD activity was decreased. Application of EPO as a component of the TDF decreased the amount of lymphocytes with early and late apoptosis signs and the contents of dienic conjugates, conjugated ketodienes and trienes, Schiff bases in plasma and lymphocytes, and catalase activity in plasma and erythrocytes, and increased the plasma activity of SOD. Conclusion. The use of EPO as a component of TDF in TI reduced lymphocyte apoptosis, activity of LP processes, and increased the plasma activity of SOD compared to the group of animals with TI without the EPO treatment.

State of the enzyme link of antioxidant protection in boys with hypoandrogenism

2020 ◽  
Vol 40 (4) ◽  
pp. 32-36
Author(s):  
L. K. Parkhomenko ◽  
◽  
L. A. Strashok ◽  
S. I. Turchina ◽  
G. V. Kosovtsova ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Free Radical ◽  
Glutathione Peroxidase ◽  
Free Radical Oxidation ◽  
Conjugated Dienes ◽  
Delayed Puberty ◽  
Control Group ◽  
Antioxidant Protection ◽  
Radical Oxidation

Recently, interest in the problem of free radical oxidation in biological membranes, which is directly related to both the normal functioning of cells and the occurrence, course and outcome of many pathological conditions, has increased again in clinical medicine. The aim was to determine the role and impact of antioxidant defense in boys with hypoandrogenism. The study involved 75 adolescents with hypoandrogenism aged 13–18 years, who underwent a complex of clinical and laboratory examinations. All patients were conducted complex of anthropometric research and determination of the degree of delayed puberty, laboratory and instrumental examination. Free radical oxidation was determined by the levels of malondialdehyde, conjugated dienes, carbonated proteins, superoxide dismutase and catalase in the serum, and restored glutathione and glutathione peroxidase in whole blood. Based on their determination, the coefficient of oxidative stress was calculated. Statistical processing of results was performed using parametric and nonparametric methods. The study of indicators of the free radical oxidation process found that adolescents with hypoandrogenism have multidirectional changes in the oxidation of proteins and lipids, namely: the level of conjugated dienes increases, the concentration of malondialdehyde remains at the level of the control group, and the level of carbonated proteins tends to decrease. As for the activity of antioxidant protection enzymes, a significant decrease in the level of glutathione peroxidase was detected, while the level of superoxide dismutase and catalase remained at the level of normative indicators. Oxidative stress accompanies and is one of the pathogenetic links in the formation or maintenance of the state of hypoandrogenism in boys. This requires the use of antioxidants, the complex of which must be selected individually.

Adaptogenic correction of free radical brain damage in subchronic exposure to sodium fl uoride

2020 ◽  
pp. 381-386
Author(s):  
A. G. Zhukova ◽  
L. G. Gorokhova ◽  
A. S. Kazitskaya ◽  
T. K. Yadykina ◽  
N. N. Mikhailova ◽  
...  
Keyword(s):  
Cerebral Cortex ◽  
Free Radical ◽  
Brain Tissue ◽  
Free Radical Oxidation ◽  
Radical Oxidation ◽  
Free Radical Processes ◽  
Subchronic Exposure ◽  
Antioxidant Defense Enzymes ◽  
Radical Processes ◽  
Complex Drug

Introduction. Fluorine compounds in small doses, but with prolonged exposure, cause various disorders in organs at the cellular and molecular levels. Activation of free-radical processes plays an important role in the damaging eff ect of fl uorides. Th erefore, one of the most eff ective ways to limit fl uorine-induced damage is to directly aff ect free-radical processes using herbal preparations with antioxidant properties.The aim of the study is to study the eff ect of a dihydroquercetin-based drug on the activity of free radical processes in brain tissue under subchronic exposure to sodium fl uoride (NaF).Materials and methods. Th e work was performed on white male laboratory rats weighing 200-250 g. Th e rats were divided into 3 groups: 1 — control; 2 — rats with chronic exposure to sodium fl uoride (NaF) for 9 weeks; 3 — rats receiving a NAF solution with simultaneous administration of a complex drug based on dihydroquercetin at a dose of 3 mg/kg in 1% starch gel for 3, 6 and 9 weeks. The activity of free radical oxidation and antioxidant defense enzymes — superoxide dismutase (SOD) and catalase-was determined in the cerebral cortex. Th e level of expression of hypoxia-induced transcription factor HIF — 1A and inducible forms of proteins HSP72 and HSP32 were determined in the cytosolic fraction of brain tissue.Results. In the early stages of subchronic fl uoride exposure (1-3 weeks), the expression of protective proteins HIF-1α, HSP72, HSP32 and catalase was shown in the rat cortex, as a result of which the activity of free-radical processes was maintained at the control level. An increase in the timing of fl uoride intake to 9 weeks led to a decrease in antioxidant protection and signifi cant activation of free radical oxidation in brain tissue. Daily administration of a complex drug with dihydroquercetin for 3, 6 and 9 weeks to rats with subchronic fl uoride exposure led to a decrease in the severity of pro- and antioxidant balance disorders in the cerebral cortex. At the same time, the greatest protective eff ect of dihydroquercetin with fl uoride exposure was manifested by the 9th week of its administration.Conclusions. When subchronic intake of fl uorides in the body, the drug based on dihydroquercetin has a neuroprotective eff ect, which is manifested by an increase in the activity of antioxidant enzymes of fr ee radical oxidation and catalase and the resistance of the cortex to induced fr ee radical oxidation.

Gender differences in biochemical measurement reflecting the state of free-radical oxidation and antioxidant protection among workers in metallurgical production

2019 ◽  
Vol 1 (10) ◽  
pp. 877-881
Author(s):  
I. A. Umnyagina ◽  
L. A. Strakhova ◽  
T. V. Blinova
Keyword(s):  
Oxidative Stress ◽  
Gender Differences ◽  
Free Radical ◽  
Biochemical Parameters ◽  
Free Radical Oxidation ◽  
The Body ◽  
Antioxidant Protection ◽  
Radical Oxidation ◽  
Metallurgical Production ◽  
Production Factors

Introduction. To date, age and sex differences have been established for many biochemical parameters. Gender differences in indicators for systems such as antioxidant, thiol-disulfide, oxidative stress and inflammation systems are absent or under study.The aim of the study was to identify gender differences in biochemical parameters reflecting the functioning of antioxidant systems of the body and free radical oxidation in workers of metallurgical production, in contact with harmful production factors.Materials and methods. The blood of men and women working at the metallurgical enterprise of the Nizhny Novgorod region (n=80) under the influence of a complex of physical and chemical production factors was studied. Total oxidative stress, total antioxidant capacity of serum, glutathione levels were studied by photometric biochemical methods. Levels of C-reactive protein and 8-hydroxy–2-deoxyguanosine were studied by ELISA.Results. The average amount of peroxides in the serum of women exceeded 1.6 times this figure in men. In the group of men, the content of 8-Ondg was higher by 26% (p=0.012), the level of GS-by 12% (p=0.019), the activity of SOD — by 1.5–2 times (p=0.0001), the level of CRP — by 2 times (p=0.008) compared to similar indicators in women.Conclusions. Studies of gender differences in workers under the influence of harmful production factors will allow more effective approach to the etiology, treatment and prognosis of production-related diseases. Indicators of oxidative stress and antioxidant protection can be indicators of the health of workers under the influence of harmful industrial factors and be important in the prevention of diseases associated with oxidative stress.

scholarly journals PSX-3 Antioxidant system characteristics in Saanen goats depending on performance

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 461-462
Author(s):  
Polina D Bokhan ◽  
Alesya A Bakhta ◽  
Larisa Y Karpenko ◽  
Anna I Kozitcyna ◽  
Anna B Balykina ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Free Radical ◽  
Milk Yield ◽  
Antioxidant System ◽  
Free Radical Oxidation ◽  
Dairy Goat ◽  
Blood Levels ◽  
Lactation Performance ◽  
Radical Oxidation ◽  
System Characteristics

Abstract Dairy goat breeding is widespread worldwide. Goat milk and other derivative products are an important part of nutrition. Dairy products are the most important part of goat breeding; therefore, lactation performance is a relevant issue. The purpose of this study was to evaluate antioxidant system characteristics in Saanen goats depending on lactation performance. The experiment was conducted in the north-western region of Russian Federation, in the laboratories of the biochemistry and physiology departments at FSBEI of Higher Education «SPbSAVM». The three experimental groups included 30 Saanen goats each, 2nd–3rd lactations, selected using matched pairs method. 1st group included low milk producing ability goats (< 600 kg of milk yield per year), 2nd group included average milk producing ability goats (600–800 kg of milk yield per year), 3rd group included high milk producing ability goats (>800 kg of milk yield per year). The blood samples were taken once: at peak lactation performance (45 days after parturition). The blood levels of lipid peroxygenation markers (malondialdehyde, dienketone and conjugated dienes) and superoxide dismutase and catalase activity were assessed by standard methods. Results presented as mean±standard error of the mean. Student’s t-test was used after proving normal distribution. Level of significance is labeled as P < 0.05. The results are displayed in table 1 and 2. After analyzing the received data of antioxidant system characteristics, we found a high intensity of free-radical oxidation in high milk producing ability goats. This results in oxidative stress development. Activity of anti-oxidizing enzymes was elevated; therefore, free-radical oxidation is intensive. Consequently, these data allow us to take into consideration exogenous anti-oxidizing agents administration in high milk producing ability goats in order to reduce oxidative stress.

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