scholarly journals Blood typing in positive DEA 1 dogs

2020 ◽  
Vol 57 (1) ◽  
pp. e151444
Author(s):  
Suzana Claudia Spinola Santos ◽  
Mariane Melo Santos ◽  
Wellington Francisco Rodrigues ◽  
Roberto Meyer ◽  
Maria de Fátima Dias Costa
Keyword(s):  
Flow Cytometry ◽  
Autosomal Dominant ◽  
Blood Typing ◽  
Negative Type ◽  
Through Flow

Blood typing techniques have been improved to ensure greater safety for transfusion procedures. Typification for the DEA 1 antigen through flow cytometry should offer more reliability to routine immunohematology in donor and recipient dogs. Currently, the DEA 1 group is starting to be an autosomal dominant allelic system with the DEA 1 negative type and its variations of positivity. The present study investigated the DEA 1 antigen using the techniques of immunochromatography, hemagglutination and flow cytometry. Among the positive animals for the DEA 1 group, typified by flow cytometry, medium intensities of fluorescence were found, which are indicative of weak, moderate and strong antigenicity. This enabled the division of the DEA 1 group into weak positive, moderate positive and strong positive. The blood typing techniques for the DEA 1 group by flow cytometry, agglutination and immunochromatography had positive (Spearman r=0.70) and statistically significant (p>0.0001) correlations.

scholarly journals THE INFLUENCE OF ELECTROMAGNETIC FIELD ON MORPHOPHYSIOLOGICAL PARAMETERS OF BACTERIAL CELLS EVALUATED THROUGH FLOW CYTOMETRY

2016 ◽  
Author(s):  
Elena Radu ◽  
◽  
F. Marinescu ◽  
I. Savin ◽  
C. M. Kamerzan (Saviuc) ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Electromagnetic Field ◽  
Bacterial Cells ◽  
Through Flow

Advances in Oceanography through Flow Cytometry

1991 ◽  
pp. 351-399 ◽  
Author(s):  
R. J. Olson ◽  
E. R. Zettler ◽  
S. W. Chisholm ◽  
J. A. Dusenberry
Keyword(s):  
Flow Cytometry ◽  
Through Flow

ABCG2/BCRP interaction with the sea grass Thalassia testudinum

2015 ◽  
Vol 30 (4) ◽  
Author(s):  
Verónica Miguel ◽  
Jon A. Otero ◽  
Borja Barrera ◽  
Idania Rodeiro ◽  
Julio G. Prieto ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Tumor Cells ◽  
Survival Rates ◽  
Ethanolic Extract ◽  
Thalassia Testudinum ◽  
Marine Plant ◽  
Through Flow ◽  
Sea Grass ◽  
Aqueous Ethanolic Extract ◽  
Mtt Assays

AbstractThe aqueous ethanolic extract from leaves of the marine plantTo evaluate the interaction of ABCG2/BCRP with the extract, studies in cells overexpressing human BCRP transporter and its murine ortholog Bcrp1 were performed.extract could be included as MDR modulator, as interaction with ABCG2/BCRP has been shown through flow cytometry and MTT assays. The cells overexpressing ABCG2/BCRP in the presence of the extract (25–150 μg/mL) decreased the survival rates of the anti-tumoral mitoxantrone. Our results support its inclusion as a possible MDR modulator against tumor cells that overexpress ABCG2/BCRP.

L'analisi del DNA mediante citometria a flusso nei tumori superficiali della vescica: DNA analysis through flow cytometry in superficial bladder tumours

1995 ◽  
Vol 62 (2) ◽  
pp. 188-195
Author(s):  
R. Pianon ◽  
A. D'Amico ◽  
M. Rahmati ◽  
A. Paganelli ◽  
D. Schiavone ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Clinical Practice ◽  
Dna Analysis ◽  
Prognostic Significance ◽  
Clinical Development ◽  
Dna Flow Cytometry ◽  
Through Flow ◽  
Data Investigation ◽  
Precise Role ◽  
Bladder Tumours

The aim of this work is to try to characterize the prognostic value of DNA flow cytometry in superficial bladder tumours and to try to indicate this investigation in clinical practice. The Authors review results reported in literature and those relative to their experience. Data investigation shows that this test has prognostic significance because it is correlated with the stage and grade of the tumour. The correlation with the clinical development of the tumour is not clear in the Authors’ experience. The Authors think that flow cytometry does not have a precise role in clinical practice.

scholarly journals CHARACTERIZATION OF MULTIPLE MYELOMA PATIENTS THROUGH FLOW CYTOMETRY AND CYTOGENETIC STUDIES 2013 – 2018

2020 ◽  
Vol 42 ◽  
pp. 254
Author(s):  
J.M. Gil-Ramos ◽  
L.M. Martínez ◽  
L. López ◽  
L.I. Jaramillo ◽  
J.D. Villegas ◽  
...  
Keyword(s):  
Multiple Myeloma ◽  
Flow Cytometry ◽  
Through Flow ◽  
Cytogenetic Studies

scholarly journals RNA Flow Cytometry for the Study of T Cell Metabolism

2021 ◽  
Vol 22 (8) ◽  
pp. 3906
Author(s):  
Alessandra Rossi ◽  
Ilenia Pacella ◽  
Silvia Piconese
Keyword(s):  
Flow Cytometry ◽  
T Cells ◽  
T Cell ◽  
Cell Metabolism ◽  
Metabolic Reprogramming ◽  
Mitochondrial Activity ◽  
Through Flow ◽  
Invaluable Tool ◽  
Epigenetic Remodeling ◽  
The One

T cells undergo activation and differentiation programs along a continuum of states that can be tracked through flow cytometry using a combination of surface and intracellular markers. Such dynamic behavior is the result of transcriptional and post-transcriptional events, initiated and sustained by the activation of specific transcription factors and by epigenetic remodeling. These signaling pathways are tightly integrated with metabolic routes in a bidirectional manner: on the one hand, T cell receptors and costimulatory molecules activate metabolic reprogramming; on the other hand, metabolites modify T cell transcriptional programs and functions. Flow cytometry represents an invaluable tool to analyze the integration of phenotypical, functional, metabolic and transcriptional features, at the single cell level in heterogeneous T cell populations, and from complex microenvironments, with potential clinical application in monitoring the efficacy of cancer immunotherapy. Here, we review the most recent advances in flow cytometry-based analysis of gene expression, in combination with indicators of mitochondrial activity, with the aim of revealing and characterizing major metabolic pathways in T cells.

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