Bioprospecção de actinobactérias associadas à esponja marinha Aplysina fulva: isolamento, caracterização e produção de compostos bioativos

he specimens of the sponges Aplysina lacunosa, Aplysina fulva, Cliona varians, Cinachyrella kuekenthali, Amphimedon viridis and Aaptos pernucleata, were collected in Mochima Bay, Venezuela, in October 2009 with the purpose of identifying families of secondary metabolites and evaluating the antibacterial properties, antifungal and brine shrimp lethal study of the ethyl acetate soluble fractions of these sponges. The chemical evaluation showed the presence of alkaloids, sterols, triterpenes, methylene ketone and tannins. The ethyl acetate soluble fractions of A. pernucleata, A. fulva and A. lacunosa sponges were the only ones that showed antibacterial activity against Staphylococcus aureus, Listeria monocytogenes, and Escherichia coli; the soluble fraction of A. lacunosa showed the highest inhibition zones. The antifungal activity was not significant, except for A. pernucleata sponge wich showed mild activity against Candida albicans. The most prominent lethal activity in Artemia salina, were observed for C. kuekenthali, A. viridis and A. pernucleata sponges, with LC50 values of 4,10, 1,13 and < 0,01µg/mL respectively. These results allow profiling the sponges A. lacunosa, A. pernucleata and A. fulva as promising sources of antimicrobial compounds and the sponges C. kuekenthali, A. viridis and A. pernucleata as sources of antitumor compounds.

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Comparison of Different Methods for Spongin-Like Collagen Extraction from Marine Sponges (Chondrilla caribensis and Aplysina fulva): Physicochemical Properties and In Vitro Biological Analysis

Membranes ◽

10.3390/membranes11070522 ◽

2021 ◽

Vol 11 (7) ◽

pp. 522

Author(s):

Tiago A. T. Araújo ◽

Amanda de Souza ◽

Alan F. Santana ◽

Anna Rafaela C. Braga ◽

Márcio R. Custódio ◽

...

Keyword(s):

Cell Proliferation ◽

Fibroblast Cell ◽

Extraction Yield ◽

Marine Sponges ◽

Fibrillar Structure ◽

Control Group ◽

Fibroblast Proliferation ◽

Antioxidant Assay ◽

Aplysina Fulva

This study aimed to compare different protocols (Protocol 1: P1; Protocol 2: P2; Protocol 3: P3; Protocol 4: P4) for the extraction of spongin-like collagen (SC) from marine sponges. The SEM micrographs demonstrated a fibrillar structure for the extracts from Chondrilla caribensis and the nodular/particulate aggregates for Aplysina fulva. FTIR showed for all samples peaks similar to collagen for both species. For C. caribensis, the extracts obtained using P2, P3, and P4 protocols presented higher values of extraction yield, TPQ, and GAGs. P2 and P4 showed higher values of SC concentration and for antioxidant analysis. For A. fulva, P2, P3, and P4 provided a higher extraction yield besides an increase in the antioxidant assay. For both species, no difference was observed for Col quantification and TPQ analysis; also, higher values of GAGs were found using P2 and P4. Fibroblast proliferation observed for C. caribensis was lower for P1 on day 1 and for P2 and P3 on day 3 (for 50%) compared to the control group. There was a significant reduction in fibroblast cell proliferation for all A. fulva extracts evaluated. It can be concluded that protocols P2 and P4 were more efficient for extracting SC from C. caribensis.

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Field experimental evaluation of secondary metabolites from marine invertebrates as antifoulants

Brazilian Journal of Biology ◽

10.1590/s1519-69842002000200015 ◽

2002 ◽

Vol 62 (2) ◽

pp. 311-320 ◽

Cited By ~ 27

Author(s):

R. C PEREIRA ◽

A. G. V. CARVALHO ◽

B. A. P. GAMA ◽

R. COUTINHO

Keyword(s):

Secondary Metabolites ◽

Field Experiments ◽

Marine Invertebrates ◽

The Other ◽

Selective Action ◽

Marine Biofouling ◽

Crude Extracts ◽

Organic Extracts ◽

Fouling Organisms ◽

Aplysina Fulva

The crude organic extracts of the endemic gorgonian Phyllogorgia dilatata and two sponge species Aplysina fulva and Mycale microsigmatosa were evaluated for anti-fouling properties through field experiments. To investigate this property in ecologically meaningful conditions, crude extracts from these invertebrates were incorporated at concentrations naturally found in these marine organisms into a stable gel used as a substratum for fouling settlement. Crude extract from A. fulva showed no significant anti-fouling property at the natural concentrations used in the field experiments. In fact, fouling organisms settled significantly more on gels treated with A. fulva extract than on the control gel. On the other hand, both M. microsigmatosa and P. dilatata yielded crude extracts that exhibited a selective action inhibiting only the settlement of barnacles. The evidences obtained here by means of field experiments can provide a basis for future development of one kind of natural antifoulant technology to prevent marine biofouling.

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