scholarly journals Field experimental evaluation of secondary metabolites from marine invertebrates as antifoulants

2002 ◽  
Vol 62 (2) ◽  
pp. 311-320 ◽  
Author(s):  
R. C PEREIRA ◽  
A. G. V. CARVALHO ◽  
B. A. P. GAMA ◽  
R. COUTINHO

The crude organic extracts of the endemic gorgonian Phyllogorgia dilatata and two sponge species Aplysina fulva and Mycale microsigmatosa were evaluated for anti-fouling properties through field experiments. To investigate this property in ecologically meaningful conditions, crude extracts from these invertebrates were incorporated at concentrations naturally found in these marine organisms into a stable gel used as a substratum for fouling settlement. Crude extract from A. fulva showed no significant anti-fouling property at the natural concentrations used in the field experiments. In fact, fouling organisms settled significantly more on gels treated with A. fulva extract than on the control gel. On the other hand, both M. microsigmatosa and P. dilatata yielded crude extracts that exhibited a selective action inhibiting only the settlement of barnacles. The evidences obtained here by means of field experiments can provide a basis for future development of one kind of natural antifoulant technology to prevent marine biofouling.

2020 ◽  
Vol 85 ◽  
pp. 197-210
Author(s):  
C Angulo-Preckler ◽  
E García-Lopez ◽  
B Figuerola ◽  
C Avila ◽  
C Cid

Organisms living in the sea are exposed to fouling by other organisms. Many benthic marine invertebrates, including sponges and bryozoans, contain natural products with antimicrobial properties, since microbes usually constitute the first stages of fouling. Extracts from 4 Antarctic sponges (Myxilla (Myxilla) mollis, Mycale tylotornota, Rossella nuda, and Anoxycalyx (Scolymastra) joubini) and 2 bryozoan species (Cornucopina pectogemma and Nematoflustra flagellata) were tested separately for antifouling properties in field experiments. The different crude extracts from these invertebrates were incorporated into a substratum gel at natural concentrations for an ecological approach. Treatments were tested by submerging plates covered by these substratum gels under water in situ during 1 lunar cycle (28 d) at Deception Island (South Shetland Islands, Antarctica). Remarkably, the butanolic extracts of M. tylotornota and C. pectogemma showed complete growth inhibition of microscopic eukaryotic organisms, one of the succession stages involved in biofouling. Our results suggest that different chemical strategies may exist to avoid fouling, although the role of chemical defenses is often species-specific. Thus, the high specificity of the microbial community attached to the coated plates seems to be modulated by the chemical cues of the crude extracts of the invertebrates tested.


Marine Drugs ◽  
2021 ◽  
Vol 19 (2) ◽  
pp. 47
Author(s):  
Justin M. McNab ◽  
Jorge Rodríguez ◽  
Peter Karuso ◽  
Jane E. Williamson

Marine invertebrates are promising sources of novel bioactive secondary metabolites, and organisms like sponges, ascidians and nudibranchs are characterised by possessing potent defensive chemicals. Animals that possess chemical defences often advertise this fact with aposematic colouration that potential predators learn to avoid. One seemingly defenceless group that can present bright colouration patterns are flatworms of the order Polycladida. Although members of this group have typically been overlooked due to their solitary and benthic nature, recent studies have isolated the neurotoxin tetrodotoxin from these mesopredators. This review considers the potential of polyclads as potential sources of natural products and reviews what is known of the activity of the molecules found in these animals. Considering the ecology and diversity of polyclads, only a small number of species from both suborders of Polycladida, Acotylea and Cotylea have been investigated for natural products. As such, confirming assumptions as to which species are in any sense toxic or if the compounds they use are biosynthesised, accumulated from food or the product of symbiotic bacteria is difficult. However, further research into the group is suggested as these animals often display aposematic colouration and are known to prey on invertebrates rich in bioactive secondary metabolites.


2019 ◽  
Vol 374 (1784) ◽  
pp. 20190203 ◽  
Author(s):  
Kenan P. Fears ◽  
Andrew Barnikel ◽  
Ann Wassick ◽  
Heonjune Ryou ◽  
Janna N. Schultzhaus ◽  
...  

Concerns about the bioaccumulation of toxic antifouling compounds have necessitated the search for alternative strategies to combat marine biofouling. Because many biologically essential minerals have deleterious effects on organisms at high concentration, one approach to preventing the settlement of marine foulers is increasing the local concentration of ions that are naturally present in seawater. Here, we used surface-active borate glasses as a platform to directly deliver ions (Na + , Mg 2+ and BO 4 3− ) to the adhesive interface under acorn barnacles ( Amphibalanus ( =Balanus ) amphitrite ). Additionally, surface-active glasses formed reaction layers at the glass–water interface, presenting another challenge to fouling organisms. Proteomics analysis showed that cement deposited on the gelatinous reaction layers is more soluble than cement deposited on insoluble glasses, indicating the reaction layer and/or released ions disrupted adhesion processes. Laboratory experiments showed that the majority (greater than 79%) of adult barnacles re-attached to silica-free borate glasses for 14 days could be released and, more importantly, barnacle larvae did not settle on the glasses. The formation of microbial biofilms in field tests diminished the performance of the materials. While periodic water jetting (120 psi) did not prevent the formation of biofilms, weekly cleaning did dramatically reduce macrofouling on magnesium aluminoborate glass to levels below a commercial foul-release coating. This article is part of the theme issue ‘Transdisciplinary approaches to the study of adhesion and adhesives in biological systems’.


1951 ◽  
Vol 41 (3) ◽  
pp. 271-281 ◽  
Author(s):  
E. S. Bunting ◽  
G. E. Blackman

Between 1942 and 1950, some thirty field experiments have been carried out in the southern half of England to assess the potential value, either for grain or forage production, of seventeen openpollinated flint or dent maizes together with twentynine single or double hybrids of American or Canadian origin.Early-maturing flint varieties will consistently ripen grain, but before mechanical threshing or storage, the cobs require drying. Sibthorp, a mass selection made from an unknown German variety, is the earliest and most productive flint maize so far tested, and in the experiments has yielded as much as 39 cwt. of grain per acre with an average of 24 cwt. The earliest American hybrids, i.e. those with a U.S.A. rating of 80 days from sowing to maturity, give very high yields of grain in favourable seasons. Within the group Wisconsin 240–275, a yield level equal to or exceeding 50 cwt./acre has on occasion been recorded. On the other hand, in the most unfavourable years, such hybrids just failed to produce ripe cobs.Attempts to maintain sixty-five parent inlines of the earliest hybrids have largely failed. However, many of the parent single crosses have matured, and the production on a field scale of the double-cross seed of both Wisconsin 240 and 255 has been carried out.Spacing experiments indicate that for optimum grain production a density of 6 plants/sq.yd. is required for both flint varieties and the earliest hybrids. A spatial arrangement of individual plants is to be preferred to that of groups or hills.American hybrids, in the class of ‘90 days’ to maturity, will in all but the most unfavourable seasons reach the ‘early-dent’ stage of the grain before the incidence of autumn frosts. Yields of dry matter of plants harvested in this phase have ranged from 30 to 85 cwt. of dry matter per acre. In these trials, the plant density was standardized at 4 plants/sq.yd. and higher densities may be demanded for optimal yields.The ratio of the ‘ear’ (that is, the cob, immature grain and enclosing leaf sheaths) to the total shoot weight at harvest varies greatly with the variety or hybrid. With White Horsetooth, the usual variety grown for fodder in England, no cobs are formed before the plants are killed by frost, while with the early hybrids, the ear may be half the weight of the whole shoot.The earliest variety Sibthorp from an early May sowing takes approximately 70–80 days to reach full anthesis, while ‘80-day’ American hybrids are a week later. From sowing to full maturity the period in England is from 140 to 160 days, thus compared to conditions in Minnesota the period is nearly twice as long. Because of the much slower rate of development and because of the humidity of English autumns, it is concluded that until the date of maturity can be advanced some 14 days, grain production on a field scale is not yet feasible. On the other hand, many of the American hybrids are well fitted to the production of silage. The greatest drawback to the introduction of such hybrids is the liability of the seed and seedlings to be attacked by rooks.


1989 ◽  
Vol 67 (10) ◽  
pp. 2904-2911 ◽  
Author(s):  
Alexander L. McIlraith ◽  
Gordon G. C. Robinson ◽  
Jennifer M. Shay

Field experiments and survey methods were used to assess competition and interaction between Lemna minor L. and Lemna trisulca L. at Delta Marsh, Manitoba. Sites were dominated by one or the other species or codominated by both. Replacement series experiments predicted codominance of L. minor and L. trisulca in an unshaded eutrophic site but predicted L. minor dominance when run for a longer time. Similar experiments conducted in a shaded eutrophic site predicted L. minor dominance. Addition series experiments showed that intraspecific and interspecific competition occurred in the unshaded site. In a eutrophic unshaded ditch, high densities of L. minor suppressed L. trisulca. In a eutrophic shaded site, high densities of L. minor and green algae inhibited L. trisulca, and in a sunny, less eutrophic site high density of each species inhibited the other. In a transplant experiment, L. minor biomass in shaded enclosures approached that found naturally in two shaded sites. Lemna trisulca persisted when shaded. Vegetative biomass trends in an unshaded eutrophic marsh ditch indicated spring and fall L. trisulca dominance and summer L. minor dominance. Shaded eutrophic sites were dominated by L. minor, whereas a less eutrophic site was dominated by L. trisulca. A model is developed to explain dominance patterns, and seasonal life-history responses are considered. Key words: Lemna, duckweed, competition, interaction, resources, light, nutrients.


Author(s):  
N.B. Afonina ◽  
A.V. Otrokov ◽  
G.Sh. Khazanovich

Most modern roadheaders of selective action are equipped with loading devices with gathering-stars, which have a simpler drive design and increased reliability. As a result of the experimental studies carried out at SRSPU (NPI), it was found that under certain conditions, it is possible to transfer large lump material of a pile into a fluidized state, which reduces the energy consumption of material loading. An increase in the number of beams of the gathering-stars reduces the influence of the other parameters of the loader. Analysis of the process of interaction of the gathering-star’s beam with the receiving conveyor’s side edge to avoid jamming of the picking gathering-star by a lump of material with the implementation of high dynamic loads, it is necessary to set the gathering-beams at an angle of at least 60 degrees against rotation.


PLoS ONE ◽  
2010 ◽  
Vol 5 (11) ◽  
pp. e13923 ◽  
Author(s):  
Séverine Van Dyck ◽  
Patrick Flammang ◽  
Céline Meriaux ◽  
David Bonnel ◽  
Michel Salzet ◽  
...  

1935 ◽  
Vol 62 (3) ◽  
pp. 289-296 ◽  
Author(s):  
M. Ruiz Castaneda

Two substances differing in immunological behavior as well as in certain chemical properties have been isolated from soluble extracts of B. proteus X-19. Both substances appear to be polysaccharides. The first substance is precipitated from X-19 crude extracts by a relatively low percentage of alcohol and electrolytes (from one to two and a half volumes of alcohol). When purified as far as possible, it gives a negative biuret reaction, a positive Molish and has a nitrogen content of 4 per cent. This material, which we call X factor, has the immunological properties of the common antigenic factor in Proteus X-19 and typhus Rickettsiae, described elsewhere. It has the property of precipitating with typhus serum as well as anti-Proteus serum, even after treatment with hot alkali. The second substance we call P factor, suggesting a material which is proper to Proteus X-19 and has nothing to do with the Weil-Felix reaction. It is obtained from the crude extracts of B. proteus X-19 by treating the fluids from which the X factor has been removed with an excess of alcohol (seven to ten volumes, according to electrolytes in solution). The purified material shows a nitrogen content of a little less than 1 per cent, gives a negative biuret and a positive Molish reaction. The P factor produces precipitates with anti-Proteus serum in considerable dilution, but has no effect on typhus serum. It is quickly destroyed on treating with alkali, a fact in accordance with the results already cited, which were obtained by White with whole extracts of B. proteus X-19. The duality of the X-19 extracts seems to be explained by the isolation of two immunologically different factors; one which is alkali-labile and which is proper to B. proteus X-19; and the other which is alkali-stable and is the common antigenic factor in Proteus X-19 and typhus Rickettsiae.


Author(s):  
Andia Chaves Fonnegra ◽  
Mateo López Victoria ◽  
Fernando Parra Velandia ◽  
Sven Zea

The Caribbean encrusting and excavating sponges Cliona aprica, C. caribbaea, C. delitrix and C. tenuis (Porifera, Hadromerida, Clionaidae), aggresively undermine and displace live coral tissue. At San Andrés island and Islas del Rosario (Colombian Caribbean), in all 145 observed cases of direct contact of the sponges C. aprica, C. caribbaea and C. tenuis with 17 coral species, corals showed unhealthy signs in their tissue. It was also noticed that the surface of these sponges is colonized by few organisms and that they are rarely preyed upon. To establish the possible use of chemical substances by these sponges in competition for space with corals (allelopathy), as inhibitors of larval settlement (antifouling), and as feeding deterrents against generalist fish (antipredatory), the activity of crude organic extracts was experimentally evaluated. Extracts were prepared in methanol and 1:2 metanol:dichloromethane and incorporated in experimental media at the natural concentration within the sponges. Using an unpublished method being developed by J. Pawlik (University of North Caroline at Wilmington) and M. Ilan (Tel Aviv University), PhytagelTM disks with crude extracts of each of the four sponge species, placed on the coral Montastrea cavernosa, produced a greater degree of polyp mortality than control gels without extract. Gels with extracts of the sponges C. aprica and C. caribbaea + C. tenuis, served in Petri dishes and used as substratum in the field, inhibited significantly the settlement of fouling organisms, in comparison to control gels. In laboratory trials, wheat flour pellets with extracts C. delitrix and C. caribbaea + C. tenuis were significantly rejected by the omnivore reef damselfish, Stegastes partitus, whereas pellets with extract of C. aprica did not deter feeding. These results suggest that substances present in the crude organic extracts of these sponges may be responsible in part for their ability to compete for reef substrata and to defend themselves from potential aggressors.


2020 ◽  
Author(s):  
Md. Mahi Imam Mollah ◽  
Yonggyun Kim

Abstract Background: Xenorhabdus and Photorhabdus are entomopathogenic bacteria that cause septicemia and toxemia in insects. They produce secondary metabolites to induce host immunosuppression. Their metabolite compositions vary among bacterial species. Little is known about the relationship between metabolite compositions and the bacterial pathogenicity. The objective of this study was to compare pathogenicity and production of secondary metabolites of 14 bacterial isolates (species or strains) of Xenorhabdus and Photorhabdus. Results: All bacterial isolates exhibited insecticidal activities after hemocoelic injection to Spodoptera exigua (a lepidopteran insect) larvae, with median lethal doses ranging from 168.8 to 641.3 CFU per larva. Bacterial infection also led to immunosuppression by inhibiting eicosanoid biosynthesis. Bacterial culture broth was fractionated into four different organic extracts. All four organic extracts of each bacterial species exhibited insecticidal activities and resulted in immunosuppression. These organic extracts were subjected to GC-MS analysis which predicted 182 compounds, showing differential compositions for 14 bacteria isolates. There were positive correlations between total number of secondary metabolites produced by each bacterial culture broth and its bacterial pathogenicity based on immunosuppression and insecticidal activity. From these correlation results, 70 virulent compounds were selected from secondary metabolites of high virulent bacterial isolates by deducting those of low virulent bacterial isolates. These selected virulent compounds exhibited significant immunosuppressive activities by inhibiting eicosanoid biosynthesis. They also exhibited relatively high insecticidal activities. Conclusion: Virulence variation between Xenorhabdus and Photorhabdus is determined by their different compositions of secondary metabolites, of which PLA2 inhibitors play a crucial role.


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