scholarly journals Seqüências ORESTES (open reading frame expressed sequence tags) de trypanosoma cruzi e transcrição de DNA satélite

2008 ◽  
Author(s):  
Camila Augusta de Oliveira Martins
Keyword(s):  
Trypanosoma Cruzi ◽  
Expressed Sequence Tags ◽  
Open Reading Frame ◽  
Reading Frame ◽  
Expressed Sequence

Characterization of open reading frame-expressed sequence tags generated from Bos indicus and B. taurus mammary gland cDNA libraries

2004 ◽  
Vol 35 (3) ◽  
pp. 213-219 ◽  
Author(s):  
A. F. Mota ◽  
T. S. Sonstegard ◽  
C. P. Van Tassell ◽  
L. L. Shade ◽  
L. K. Matukumalli ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Expressed Sequence Tags ◽  
Bos Indicus ◽  
Open Reading Frame ◽  
Cdna Libraries ◽  
Reading Frame ◽  
Expressed Sequence

scholarly journals Expressed Sequence Tags (ESTs) from a Jute (Corchorus olitorius) cDNA Library

1970 ◽  
Vol 16 (2) ◽  
pp. 95-104 ◽  
Author(s):  
J Matthew Taliaferro ◽  
Ahmad S Islam ◽  
Kanagasabapathi Sathasivan
Keyword(s):  
Arabidopsis Thaliana ◽  
Cdna Library ◽  
Expressed Sequence Tags ◽  
Higher Plants ◽  
Open Reading Frame ◽  
Sequence Information ◽  
Corchorus Olitorius ◽  
Reading Frame ◽  
Partial Cdna ◽  
Expressed Sequence

Analysis of the genome of jute (Corchorus olitorius) was done by creating a new cDNA library of expressed sequence tags (ESTs) in pBluescript as the previous libraries reported earlier in this journal yielded only small DNA fragments from chloroplast and mitochondrial DNA. This report discusses results from a cDNA library constructed using poly A+ mRNA purified from 7-day old etiolated jute seedlings. Out of 700 recombinant plasmids obtained, 250 were analyzed using WU-BLAST (www.arabidopsis.org) for similar EST sequences in Arabidopsis thaliana and other higher plants. So far the analysis of the library has yielded several significant sequences, including the complete open reading frame of the 60S acidic ribosomal protein P3 and a partial cDNA of Class I chitinase. These results and future EST sequences from this library will be made available in Genbank and the sequence information will be used to clone full length DNA through PCR.  DOI = 10.3329/ptcb.v16i2.1110Plant Tissue Cult. & Biotech. 16(2): 95-104, 2006 (December)

Generation and analysis of expressed sequence tags from Trypanosoma cruzi trypomastigote and amastigote cDNA libraries

2004 ◽  
Vol 136 (2) ◽  
pp. 221-225 ◽  
Author(s):  
Fernán Agüero ◽  
Karim Ben Abdellah ◽  
Valeria Tekiel ◽  
Daniel O. Sánchez ◽  
Antonio González
Keyword(s):  
Trypanosoma Cruzi ◽  
Expressed Sequence Tags ◽  
Cdna Libraries ◽  
Expressed Sequence

scholarly journals Analysis of expressed sequence tags from Trypanosoma cruzi amastigotes

2005 ◽  
Vol 100 (4) ◽  
pp. 385-389 ◽  
Author(s):  
Gustavo C Cerqueira ◽  
Wanderson D DaRocha ◽  
Priscila C Campos ◽  
Cláudia S Zouain ◽  
Santuza MR Teixeira
Keyword(s):  
Trypanosoma Cruzi ◽  
Expressed Sequence Tags ◽  
Expressed Sequence

scholarly journals Gene Discovery through Expressed Sequence Tag Sequencing in Trypanosoma cruzi

1998 ◽  
Vol 66 (11) ◽  
pp. 5393-5398 ◽  
Author(s):  
Ramiro E. Verdun ◽  
Nelson Di Paolo ◽  
Turan P. Urmenyi ◽  
Edson Rondinelli ◽  
Alberto C. C. Frasch ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Expressed Sequence Tags ◽  
Vaccine Development ◽  
Expressed Sequence Tag ◽  
Genome Project ◽  
Gene Identification ◽  
Biological Functions ◽  
Human Pathogens ◽  
Surface Molecules ◽  
Expressed Sequence

ABSTRACT Analysis of expressed sequence tags (ESTs) constitutes a useful approach for gene identification that, in the case of human pathogens, might result in the identification of new targets for chemotherapy and vaccine development. As part of the Trypanosoma cruzigenome project, we have partially sequenced the 5′ ends of 1,949 clones to generate ESTs. The clones were randomly selected from a normalized CL Brener epimastigote cDNA library. A total of 14.6% of the clones were homologous to previously identified T. cruzi genes, while 18.4% had significant matches to genes from other organisms in the database. A total of 67% of the ESTs had no matches in the database, and thus, some of them might be T. cruzi-specific genes. Functional groups of those sequences with matches in the database were constructed according to their putative biological functions. The two largest categories were protein synthesis (23.3%) and cell surface molecules (10.8%). The information reported in this paper should be useful for researchers in the field to analyze genes and proteins of their own interest.

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