scholarly journals Biomphalaria glabrata transcriptome: Identification of cell-signalling, transcriptional control and immune-related genes from open reading frame expressed sequence tags (ORESTES)

2007 ◽  
Vol 31 (8) ◽  
pp. 763-782 ◽  
Author(s):  
Anne E. Lockyer ◽  
Jennifer N. Spinks ◽  
Anthony J. Walker ◽  
Richard A. Kane ◽  
Leslie R. Noble ◽  
...  
Keyword(s):  
Expressed Sequence Tags ◽  
Transcriptional Control ◽  
Cell Signalling ◽  
Biomphalaria Glabrata ◽  
Open Reading Frame ◽  
Reading Frame ◽  
Immune Related Genes ◽  
Expressed Sequence

Characterization of open reading frame-expressed sequence tags generated from Bos indicus and B. taurus mammary gland cDNA libraries

2004 ◽  
Vol 35 (3) ◽  
pp. 213-219 ◽  
Author(s):  
A. F. Mota ◽  
T. S. Sonstegard ◽  
C. P. Van Tassell ◽  
L. L. Shade ◽  
L. K. Matukumalli ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Expressed Sequence Tags ◽  
Bos Indicus ◽  
Open Reading Frame ◽  
Cdna Libraries ◽  
Reading Frame ◽  
Expressed Sequence

scholarly journals Expressed Sequence Tags (ESTs) from a Jute (Corchorus olitorius) cDNA Library

1970 ◽  
Vol 16 (2) ◽  
pp. 95-104 ◽  
Author(s):  
J Matthew Taliaferro ◽  
Ahmad S Islam ◽  
Kanagasabapathi Sathasivan
Keyword(s):  
Arabidopsis Thaliana ◽  
Cdna Library ◽  
Expressed Sequence Tags ◽  
Higher Plants ◽  
Open Reading Frame ◽  
Sequence Information ◽  
Corchorus Olitorius ◽  
Reading Frame ◽  
Partial Cdna ◽  
Expressed Sequence

Analysis of the genome of jute (Corchorus olitorius) was done by creating a new cDNA library of expressed sequence tags (ESTs) in pBluescript as the previous libraries reported earlier in this journal yielded only small DNA fragments from chloroplast and mitochondrial DNA. This report discusses results from a cDNA library constructed using poly A+ mRNA purified from 7-day old etiolated jute seedlings. Out of 700 recombinant plasmids obtained, 250 were analyzed using WU-BLAST (www.arabidopsis.org) for similar EST sequences in Arabidopsis thaliana and other higher plants. So far the analysis of the library has yielded several significant sequences, including the complete open reading frame of the 60S acidic ribosomal protein P3 and a partial cDNA of Class I chitinase. These results and future EST sequences from this library will be made available in Genbank and the sequence information will be used to clone full length DNA through PCR.  DOI = 10.3329/ptcb.v16i2.1110Plant Tissue Cult. & Biotech. 16(2): 95-104, 2006 (December)

scholarly journals Sequence analysis and transformation by the catabolic 3-dehydroquinase (QUTE) gene from Aspergillus nidulans

1986 ◽  
Vol 240 (2) ◽  
pp. 481-488 ◽  
Author(s):  
A J Da Silva ◽  
H Whittington ◽  
J Clements ◽  
C Roberts ◽  
A R Hawkins
Keyword(s):  
Amino Acids ◽  
Aspergillus Nidulans ◽  
Sole Carbon Source ◽  
Transcriptional Control ◽  
Quinic Acid ◽  
Open Reading Frame ◽  
Amino Acid Homology ◽  
Reading Frame ◽  
Vector Sequences ◽  
Fungal Promoters

The induction of catabolic 3-dehydroquinase by quinic acid in Aspergillus nidulans has been shown to involve transcriptional control and yields a single major 0.8 kb mRNA. The nucleotide sequence of the catabolic 3-dehydroquinase QUTE gene has been determined and contains a single uninterrupted open reading frame of 462 bases encoding a 16,505 Da protein of 153 residues. Comparison with the corresponding QA2 gene of Neurospora crassa reveals the absence of 75 nucleotides encoding 25 amino acids from the centre of the QUTE gene of A. nidulans and the presence of 21 additional nucleotides at its 3′ end. There is no nucleotide or amino acid homology between these two elements. A 16 bp inverted repeat (5′ GGCAGAGCGTTCTGCC) shows similarity to such repeats found in other fungal promoters. The functional integrity of the QUTE gene was demonstrated by the transformation of a qutE mutant strain which regains growth on quinic acid as sole carbon source. Four of the twelve transformed strains examined contained vector sequences integrated at the qutE locus, and these strains all exhibited normal regulation of 3-dehydroquinase even when 16 copies of the QUTE gene were present.

scholarly journals ComEB protein is dispensable for transformation but must be translated for optimal synthesis of ComEC

2020 ◽  
Author(s):  
Micaela De Santis ◽  
Jeanette Hahn ◽  
David Dubnau
Keyword(s):  
Bacillus Subtilis ◽  
Transcriptional Control ◽  
Cytidine Deaminase ◽  
Open Reading Frame ◽  
Common Mechanism ◽  
Its Sequence ◽  
Dna Uptake ◽  
Reading Frame ◽  
Ribosomal Binding Site

We show that the ComEB protein is not required for transformation in Bacillus subtilis, despite its expression from within the comE operon under competence control. We show further that the synthesis of the putative channel protein ComEC is translationally coupled to the upstream comEB open reading frame, so that translation of comEB and a suboptimal ribosomal binding site embedded in its sequence are needed for proper comEC expression. Translational coupling appears to be a common mechanism in three major competence operons for the adjustment of protein amounts independent of transcriptional control, probably ensuring the correct stoichiometries for assembly of the transformation machinery. comEB and comFC respectively encode cytidine deaminase and a protein resembling type 1 phosphoribosyl transferases and we speculate that nucleotide scavenging proteins are produced under competence control for efficient reutilization of the products of degradation of the non-transforming strand during DNA uptake.

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