Osteoblastic Differentiation of Human Stem Cells Derived from Bone Marrow and Periodontal Ligament Under the Effect of Enamel Matrix Derivative and Transforming Growth Factor-Beta

2013 ◽  
Vol 28 (6) ◽  
pp. e440-e450 ◽  
Author(s):  
Behzad Houshmand ◽  
Hossein Behnia ◽  
Ahad Khoshzaban ◽  
Golnaz Morad ◽  
Gholamreza Behrouzi ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Transforming Growth Factor Beta ◽  
Periodontal Ligament ◽  
Transforming Growth Factor ◽  
Osteoblastic Differentiation ◽  
Enamel Matrix Derivative ◽  
Enamel Matrix ◽  
Human Stem Cells ◽  
Growth Factor Beta ◽  
Matrix Derivative

scholarly journals Enamel Matrix Derivative and TGF-Beta 1 Target Genes in Human Tongue Carcinoma Cells

2020 ◽  
pp. 1-8
Author(s):  
Matti Mauramo ◽  
Matti Mauramo ◽  
Suvi-Tuuli Vilen ◽  
Timo Sorsa ◽  
Tuula Salo
Keyword(s):  
Transforming Growth Factor Beta ◽  
Target Genes ◽  
Transforming Growth Factor ◽  
Carcinoma Cells ◽  
Oral Carcinoma ◽  
Enamel Matrix Derivative ◽  
Enamel Matrix ◽  
Tgf Beta ◽  
Human Tongue ◽  
Matrix Derivative

Enamel matrix derivative (EMD) can enhance proliferation and migration of different oral cell lines, including malignant oral carcinoma cells, in vitro and in vivo. The composition of EMD is not known, but part of the effects have been postulated to be caused by transforming growth factor-beta-1 (TGF-beta 1). This study aimed to compare target genes of EMD and TGF-beta 1 on highly malignant oral carcinoma HSC-3 cells. Microarrays were used to examine differentially expressed genes in HSC-3 cells after 6h and 24h incubations with EMD (200 µg/ml) or TGF-beta 1 (10 ng/ml). Gene Ontology (GO) enrichment analysis of the regulated genes was also conducted. After 6h and 24h of EMD treatments 42 and 12 genes, respectively, were statistically significantly (P<0.05) up- or down-regulated. However, as many as 393 and 346 genes were statistically significantly (P<0.05) up- or down-regulated by TGF-beta 1. Among the most up-regulated genes by both of the study reagents were MMP-9 and -10. The expression of MMP-10 by EMD treated carcinoma cells was also verified in protein level. In conclusion, TGF-beta 1 regulates more and mostly different genes compared with EMD, but both regulate the expression of matrix metalloproteinase genes in oral carcinoma cells.

Effects of enamel matrix derivative and transforming growth factor-?1 on human periodontal ligament fibroblasts

2007 ◽  
Vol 34 (6) ◽  
pp. 514-522 ◽  
Author(s):  
Thaisângela L. S. Rodrigues ◽  
Julie T. Marchesan ◽  
Ricardo D. Coletta ◽  
Arthur B. Novaes ◽  
Márcio F. de M. Grisi ◽  
...  
Keyword(s):  
Growth Factor ◽  
Periodontal Ligament ◽  
Transforming Growth Factor ◽  
Enamel Matrix Derivative ◽  
Enamel Matrix ◽  
Periodontal Ligament Fibroblasts ◽  
Human Periodontal Ligament Fibroblasts ◽  
Matrix Derivative

The Influence of Enamel Matrix Derivative Associated With Insulin-Like Growth Factor-I on Periodontal Ligament Fibroblasts

2004 ◽  
Vol 75 (4) ◽  
pp. 498-504 ◽  
Author(s):  
Daniela B. Palioto ◽  
Ricardo D. Coletta ◽  
Edgard Graner ◽  
Julio Cesar Joly ◽  
Antonio Fernando Martorelli de Lima
Keyword(s):  
Growth Factor ◽  
Periodontal Ligament ◽  
Enamel Matrix Derivative ◽  
Insulin Like Growth Factor ◽  
Enamel Matrix ◽  
Periodontal Ligament Fibroblasts ◽  
Factor I ◽  
Growth Factor I ◽  
Matrix Derivative

scholarly journals Transforming growth factor beta directly regulates primitive murine hematopoietic cell proliferation

Blood ◽  
1990 ◽  
Vol 75 (3) ◽  
pp. 596-602 ◽  
Author(s):  
JR Keller ◽  
IK Mcniece ◽  
KT Sill ◽  
LR Ellingsworth ◽  
PJ Quesenberry ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Transforming Growth Factor Beta ◽  
Transforming Growth Factor ◽  
Bone Marrow Cells ◽  
Dependent Manner ◽  
Tgf Beta ◽  
Hematopoietic Progenitor ◽  
Growth Factor Beta ◽  
Dose Dependent

Abstract We previously reported that transforming growth factor beta (TGF-beta) selectively inhibits colony-stimulating factor-driven hematopoietic progenitor cell growth. We report here that TGF-beta 1 can act directly on hematopoietic progenitors to inhibit the growth of the most primitive progenitors measurable in vitro. Highly enriched populations of hematopoietic progenitor cells were obtained by isolating lineage negative (Lin-), Thy-1-positive (Thy-1+) fresh bone marrow cells, or by isolating cells from interleukin-3 (IL-3) supplemented bone marrow cultures expressing Thy-1 antigen with the fluorescent activated cell sorter. TGF-beta 1 inhibited IL-3-induced Thy-1 expression on Thy-1- negative (Thy-1-) bone marrow cells in a dose-dependent manner with an ED50 of 5 to 10 pmol/L. In addition, TGF-beta 1 inhibited the formation of multipotent and mixed colonies by isolated Thy-1+ cells, while single lineage granulocyte and macrophage colonies were not affected. The growth of Thy-1+ Lin- cells incubated as single cells in Terasaki plates in medium supplemented with IL-3 were inhibited by TGF-beta, demonstrating a direct inhibitory effect. Hematopoietic stem cells, which have a high proliferative potential (HPP) when responding to combinations of growth factors in vitro, have been detected in the bone marrow of normal mice and mice surviving a single injection of 5- fluorouracil. TGF-beta 1 inhibited the growth of all subpopulations of HPP colony forming cells (CFC) in a dose-dependent manner with an ED50 of 5 to 10 pmol/L. Thus, TGF-beta directly inhibits the growth of the most immature hematopoietic cells measurable in vitro.

Sign in / Sign up

Export Citation Format

Share Document