scholarly journals DNA barcode reveals the occurrence of Palearctic Olepa schleini Witt et al., 2005 (Lepidoptera: Erebidae: Arctiinae) from peninsular India with morphological variations and a new subspecies

2020 ◽  
Vol 12 (9) ◽  
pp. 16143-16152
Author(s):  
Aparna Sureshchandra Kalawate ◽  
Shital Pawara ◽  
A. Shabnam ◽  
K.P. Dinesh
Keyword(s):  
Dna Barcoding ◽  
New Record ◽  
Dna Barcode ◽  
Mt Dna ◽  
Peninsular India ◽  
New Subspecies ◽  
Morphological Variations ◽  
Morphological Studies ◽  
Tiger Moth ◽  
Species Groups

The present study was taken up to report a new record of the tiger moth genus, Olepa Watson, 1980 from India along with the discovery of a new subspecies.  Earlier  the genus was thought to have restricted distribution range in South and South-East Asia until the report of O. schleini Witt, Müller, Kravchenko, Miller, Hausmann & Speidel from the Mediterranean Coastal Plain of Israel in 2005. The species identification and the new subspecies is proposed based on the combination of morphological studies, available literature comparisons, geographical distribution,  DNA barcoding and its phylogeny. Morphological character crypticity and genital structure variations are well documented in the genus with ‘bio-species’ groups. DNA Barcoding data of mt COI has provided some resolution in sorting the problems of ‘bio-species’ groups of the genus in the past studies. In the present study, with the available  mt DNA COI barcodes and newly generated barcodes genetic identity is confirmed for the species O. ricini, O. schleini, O. toulgoeti and Olepa schleini chandrai ssp. nov., with their phylogenetic relationships. Morphological variations within the O. schleini species complex are discussed with a new record of the species for India and a new subspecies description. With the first mt COI barcode phylogeny for the genus, comments are made on the taxonomic identity of the mt COI DNA barcodes available in the GenBank for the Olepa species from India.  

scholarly journals Multi-locus DNA barcoding identifies matK as a suitable marker for species identification in Hibiscus L.

Genome ◽  
2016 ◽  
Vol 59 (12) ◽  
pp. 1150-1156 ◽  
Author(s):  
Sundar Poovitha ◽  
Nithaniyal Stalin ◽  
Raju Balaji ◽  
Madasamy Parani
Keyword(s):  
Dna Barcoding ◽  
Species Identification ◽  
Dna Barcode ◽  
Species Differentiation ◽  
Molecular Method ◽  
Peninsular India ◽  
Plant Materials ◽  
Medicinal Value ◽  
Formed Part ◽  
Suitable Marker

The genus Hibiscus L. includes several taxa of medicinal value and species used for the extraction of natural dyes. These applications require the use of authentic plant materials. DNA barcoding is a molecular method for species identification, which helps in reliable authentication by using one or more DNA barcode marker. In this study, we have collected 44 accessions, representing 16 species of Hibiscus, distributed in the southern peninsular India, to evaluate the discriminatory power of the two core barcodes rbcLa and matK together with the suggested additional regions trnH-psbA and ITS2. No intraspecies divergence was observed among the accessions studied. Interspecies divergence was 0%–9.6% with individual markers, which increased to 0%–12.5% and 0.8%–20.3% when using two- and three-marker combinations, respectively. Differentiation of all the species of Hibiscus was possible with the matK DNA barcode marker. Also, in two-marker combinations, only those combinations with matK differentiated all the species. Though all the three-marker combinations showed 100% species differentiation, species resolution was consistently better when the matK marker formed part of the combination. These results clearly showed that matK is more suitable when compared to rbcLa, trnH-psbA, and ITS2 for species identification in Hibiscus.

scholarly journals DNA Barcode for the Parthenogenetic Surinam Cockroach Pycnoscelus surinamensis (Linnaeus, 1758) (Blattodea: Blaberidae) from India

2022 ◽  
Vol 7 (1) ◽  
pp. 1-7
Author(s):  
A Shabnam ◽  
K P Dinesh
Keyword(s):  
Dna Barcoding ◽  
Molecular Identification ◽  
Western Ghats ◽  
Dna Barcode ◽  
Insect Fauna ◽  
Mt Dna ◽  
Faunal Diversity ◽  
Southern Western Ghats

DNA Barcoding is one of the emerging tools in molecular identification of faunal diversity, specifically insect fauna. The Surinam cockroach, Pycnoscelus surinamensis is the only known roach to be obligatorily parthenogenetic, with reported haplotypes. P. surinamensis is well established in Indomalayan, tropical and subtropical regions and substantially documented from India with a phenetic approach. Herewith we report the first set of mt DNA barcode from a vouchered collection for the species from southern Western Ghats India. Discussions are made on the identity of two sequences each of Blatteria species and Pycnoscelus species reported from USA.

scholarly journals Short Communication: Phylogenetic analysis and molecular identification of Canar (Smilax spp.) in Java, Indonesia Based on DNA Barcoding Analysis

2018 ◽  
Vol 19 (2) ◽  
pp. 364-368
Author(s):  
LULUT DWI SULISTYANINGSIH ◽  
MARLINA ARDIYANI ◽  
ABINAWANTO ABINAWANTO ◽  
ANDI SALAMAH
Keyword(s):  
Phylogenetic Analysis ◽  
Dna Barcoding ◽  
Molecular Identification ◽  
Short Communication ◽  
Pharmacological Study ◽  
Dna Barcode ◽  
Phylogenetic Reconstruction ◽  
Steroidal Saponin ◽  
Morphological Variations ◽  
Molecular Approaches

Sulistyaningsih LD, Abinawanto, Ardiyani M, Salamah A. 2018. Short Communication: Phylogenetic analysis and molecular identification of Canar (Smilax spp.) in Java, Indonesia Based on DNA Barcoding Analysis. Biodiversitas 19: 364-368. Smilax spp. (Smilacaceae) has long been used as medicinal herbs especially in East Asia and North America as they were known to be rich in steroidal saponin. Pharmacological study has been carried out in Indonesia. This genus is widespread in Indonesia and fairly abundant in Java and has been known either as edible fruit or medicinal plants. Characteristics of Smilax as a dioecious plant with high morphological variations make it thorny in species identification. Various molecular approaches have been devised to overcome identification problems such as DNA barcoding. This study, therefore was conducted to analyze the DNA barcoding application for phylogenetic and identification of Smilax in Java. A total of 31 samples were used in this study including 19 accession numbers from NCBI GeneBank. The genus Ripogonum was used as the out-group in phylogenetic reconstruction. Samples were successfully extracted by CTAB method with some modifications. rbcL region was used as the DNA barcode showed sufficient variation and conserved flanks. Two unidentified specimens have high similarity with S. leucophyla and lies in the same clade. The phylogenetic tree constructed by Maximum Likelihood analysis. The result showed that the monophyletic of Smilacaceae consisted of four clades. The genus Heterosmilax nested with Smilax though with low bootstraps value. It supports the monogeneric status of Smilacaceae.

DNA barcoding unravels three new species and a subspecies of Olepa Watson, 1980 (Lepidoptera, Erebidae, Arctiinae) from India, with morphotypes

2020 ◽  
Vol 19 (2) ◽  
pp. 44-60
Author(s):  
APARNA SURESHCHANDRA KALAWATE ◽  
K. P. DINESH ◽  
A. SHABNAM
Keyword(s):  
New Species ◽  
Dna Barcoding ◽  
Dna Sequences ◽  
Dna Barcode ◽  
Coi Gene ◽  
Male And Female ◽  
Morphological Variations ◽  
Three New Species ◽  
First Time ◽  
Respective Species

The genus Olepa is distributed in Palearctic and Oriental regions with more species in India and Sri Lanka. In the recent studies, morphological variations within the group were well established, with couple of first set of mt COI DNA barcodes for at least three species. In the present account, three new species and a new subspecies are described from the northern Western Ghats region of Maharashtra based on mt COI DNA barcode studies. Due to high morphological divergence and complete genetic homogeneity on the mt COI DNA, four morphotypes under two species are reported. Morphological and genital characters of male and female are provided along with their respective species morphotypes for the first time under this genus from India. The genitalia and the habitus of male and female are illustrated. Preliminary phylogenetic tree based on the mt COI DNA sequences available in the GenBank for the genus with the sequences for the new species also provided and discussed. Key words: new taxa, morphotype, Maharashtra, DNA barcoding, mt COI gene

DNA barcoding as an aid for species identification in austral black flies (Insecta: Diptera: Simuliidae)

Genome ◽  
2017 ◽  
Vol 60 (4) ◽  
pp. 348-357 ◽  
Author(s):  
Luis M. Hernández-Triana ◽  
Fernanda Montes De Oca ◽  
Sean W.J. Prosser ◽  
Paul D.N. Hebert ◽  
T. Ryan Gregory ◽  
...  
Keyword(s):  
Dna Barcoding ◽  
Species Identification ◽  
Dna Barcode ◽  
Distinct Species ◽  
Coi Gene ◽  
Black Flies ◽  
Cryptic Diversity ◽  
Dna Barcodes ◽  
Identification Of Species ◽  
Species Groups

In this paper, the utility of a partial sequence of the COI gene, the DNA barcoding region, for the identification of species of black flies in the austral region was assessed. Twenty-eight morphospecies were analyzed: eight of the genus Austrosimulium (four species in the subgenus Austrosimulium s. str., three species in the subgenus Novaustrosimulium, and one species unassigned to subgenus), two of the genus Cnesia, eight of Gigantodax, three of Paracnephia, one of Paraustrosimulium, and six of Simulium (subgenera Morops, Nevermannia, and Pternaspatha). The neighbour-joining tree derived from the DNA barcode sequences grouped most specimens according to species or species groups recognized by morphotaxonomic studies. Intraspecific sequence divergences within morphologically distinct species ranged from 0% to 1.8%, while higher divergences (2%–4.2%) in certain species suggested the presence of cryptic diversity. The existence of well-defined groups within S. simile revealed the likely inclusion of cryptic diversity. DNA barcodes also showed that specimens identified as C. dissimilis, C. nr. pussilla, and C. ornata might be conspecific, suggesting possible synonymy. DNA barcoding combined with a sound morphotaxonomic framework would provide an effective approach for the identification of black flies in the region.

Redescription of Agistemus lobatus Ehara, 1964 and A. terminalis (Quayle, 1912) (Acari: Trombidiformes: Stigmaeidae) with DNA barcoding

2019 ◽  
Vol 24 (1) ◽  
pp. 33
Author(s):  
Mohamed W. Negm ◽  
Tetsuo Gotoh
Keyword(s):  
Cytochrome C ◽  
Dna Barcoding ◽  
Cytochrome C Oxidase ◽  
Dna Barcode ◽  
Predatory Mites ◽  
Mitochondrial Cytochrome ◽  
Morphological Variations ◽  
Oxidase Subunit ◽  
Mitochondrial Cytochrome C

Agistemus lobatus Ehara, 1964 and A. terminalis (Quayle, 1912) (Stigmaeidae) are cosmopolitan predatory mites and appear on various plants in Japan. They are so close morphologically and can be mainly separated based on the lengths of dorsal setae sci and c2. The present study aimed to redescribe these two species for detailed morphological variations and generate sequences of partial mitochondrial cytochrome c oxidase subunit I (COI) as DNA barcode data. Our results with DNA barcoding and morphology well separated A. lobatus from the close species A. terminalis.

scholarly journals Differentiation of Mitragyna speciosa, a narcotic plant, from allied Mitragyna species using DNA barcoding-high-resolution melting (Bar-HRM) analysis

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Chayapol Tungphatthong ◽  
Santhosh Kumar J. Urumarudappa ◽  
Supita Awachai ◽  
Thongchai Sooksawate ◽  
Suchada Sukrong
Keyword(s):  
High Resolution ◽  
Dna Barcoding ◽  
High Resolution Melting ◽  
Dna Barcode ◽  
Herbal Medicines ◽  
Efficient Tool ◽  
Hrm Analysis ◽  
Mitragyna Speciosa ◽  
Leaf Powder

AbstractMitragyna speciosa (Korth.) Havil. [MS], or “kratom” in Thai, is the only narcotic species among the four species of Mitragyna in Thailand, which also include Mitragyna diversifolia (Wall. ex G. Don) Havil. [MD], Mitragyna hirsuta Havil. [MH], and Mitragyna rotundifolia (Roxb.) O. Kuntze [MR]. M. speciosa is a tropical tree belonging to the Rubiaceae family and has been prohibited by law in Thailand. However, it has been extensively covered in national and international news, as its abuse has become more popular. M. speciosa is a narcotic plant and has been used as an opium substitute and traditionally used for the treatment of chronic pain and various illnesses. Due to morphological disparities in the genus, the identification of plants in various forms, including fresh leaves, dried leaf powder, and finished products, is difficult. In this study, DNA barcoding combined with high-resolution melting (Bar-HRM) analysis was performed to differentiate M. speciosa from allied Mitragyna and to assess the capability of Bar-HRM assays to identify M. speciosa in suspected kratom or M. speciosa-containing samples. Bar-HRM analysis of PCR amplicons was based on the ITS2, rbcL, trnH-psbA, and matK DNA barcode regions. The melting profiles of ITS2 amplicons were clearly distinct, which enabled the authentication and differentiation of Mitragyna species from allied species. This study reveals that DNA barcoding coupled with HRM is an efficient tool with which to identify M. speciosa and M. speciosa-containing samples and ensure the safety and quality of traditional Thai herbal medicines.

A revision of the genus Morphostenophanes Pic, 1925 (Coleoptera, Tenebrionidae, Stenochiinae, Cnodalonini)

Zootaxa ◽  
2020 ◽  
Vol 4769 (1) ◽  
pp. 1-81
Author(s):  
DE-YAO ZHOU
Keyword(s):  
New Species ◽  
Identification Key ◽  
New Subspecies ◽  
Species Groups ◽  
North Vietnam ◽  
First Time ◽  
Coleoptera Tenebrionidae

The genus Morphostenophanes Pic, 1925 is redefined and revised. Seventeen new species and three new subspecies are described, including M. aenescens yelang Zhou, new subspecies, M. bannaensis Zhou, new species, M. brevigaster Zhou, new species, M. chongli Zhou, new species, M. crassus Zhou, new species, M. furvus Zhou, new species, M. furvus weishanus Zhou, new subspecies, M. gaoligongensis Zhou, new species, M. iridescens Zhou, new species, M. lincangensis Zhou, new species, M. linglong Zhou, new species, M. metallicus Zhou, new species, M. minor, Zhou, new species, M. planus Zhou, new species, M. purpurascens Zhou, new species, M. sinicus Zhou, new species, and M. yunnanus Zhou, new species from Yunnan, China; M. chongli glaber Zhou, new subspecies from Yunnan, China and North Vietnam; M. curvitibialis Zhou, new species from Guangxi, China, and M. luoxiaoshanus Zhou, new species from Hunan, Hubei and Jiangxi, China. M. birmanicus (Kaszab, 1980) is recorded from China (Yunnan) for the first time. M. papillatus Kaszab, 1941 is firstly recorded from Yunnan and Sichuan, China. Two poorly known species, M. aenescens Pic, 1925 and M. vietnamicus Kaszab, 1980 are redescribed. Male of M. atavus (Kaszab, 1960) is described in detail. Misidentifications of M. aenescens Pic. 1925 and M. atavus (Kaszab, 1960) in previous works are corrected. Six species groups are proposed. A species catalog, identification key and distributional maps for the genus are given. 

A REVISION OF THE SYSTEMATICS OF ACALYMMA SENSU STRICTO BARBER (COLEOPTERA: CHRYSOMELIDAE) FROM NORTH AMERICA INCLUDING MEXICO

1980 ◽  
Vol 112 (S112) ◽  
pp. 1-92 ◽  
Author(s):  
D.D. Munroe ◽  
Ray F. Smith
Keyword(s):  
New Species ◽  
North America ◽  
Male Genitalia ◽  
Sensu Stricto ◽  
The Other ◽  
New Subspecies ◽  
Distribution Maps ◽  
Species Groups

AbstractThe systematics of Acalymma sensu stricto of North America including Mexico are revised. Acalymma sensu stricto is defined and distinguished from the other species groups of Acalymma. Sixteen species are discussed including four new species: A. blomorum, A. palomarense, A. invenustum, and A. luridifrons all from Mexico. Three new subspecies of A. blandulum (LeConte) are described: blandulum (LeConte) new status, nigriventre, and yucatanense. Acalymma coruscum costaricense Bechyné is placed as a synonym of A. innubum (Fabricius). Keys are presented to all species and subspecies. Habitus and male genitalia drawings are given for all species and distribution maps are given where appropriate.

Pteris multifida— A new record from Peninsular India

2010 ◽  
Vol 52 (3-4) ◽  
pp. 425-427
Author(s):  
V. Sreenivas ◽  
P. Madhusoodanan
Keyword(s):  
New Record ◽  
Peninsular India ◽  
Pteris Multifida
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