Mevalonate-Dependent Inhibition of Transendothelial Migration and Chemotaxis of Human Peripheral Blood Neutrophils by Pravastatin

1997 ◽  
Vol 81 (6) ◽  
pp. 963-969 ◽  
Author(s):  
Stefan Dunzendorfer ◽  
Dorothea Rothbucher ◽  
Peter Schratzberger ◽  
Norbert Reinisch ◽  
Christian M. Kähler ◽  
...  
Keyword(s):  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Transendothelial Migration ◽  
Dependent Inhibition ◽  
Blood Neutrophils

scholarly journals Celastrol attenuates fMLP-induced superoxide anion generation, myeloperoxidase production, and elastase release by human neutrophils

2021 ◽  
Vol 18 (9) ◽  
pp. 1805-1809
Author(s):  
Nipapan Malisorn ◽  
Ammara Chaikan
Keyword(s):  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Human Neutrophils ◽  
Superoxide Generation ◽  
Xtt Assay ◽  
Dependent Inhibition ◽  
Blood Neutrophils ◽  
Ic50 Values ◽  
Dose Dependent Inhibition ◽  
Anti Inflammatory

Purpose: To investigate the anti-inflammatory effect of celastrol via attenuation of formyl-methionylleucyl-phenylalanine (fMLP)-induced superoxide generation, myeloperoxidase production, and elastase release by peripheral blood neutrophils. Methods: Cytotoxicity of celastrol on human peripheral blood neutrophils was investigated using a 2Htetrazolium hydroxide (XTT) assay. Human neutrophils were stimulated with 100-nM fMLP; the effect of celastrol on superoxide generation was determined via ferricytochrome C reduction, the effect on myeloperoxidase production by tetramethylbenzidine oxidation, and the effect on elastase activity by Boc-Ala-ONp hydrolysis. Results: Treatment of human neutrophils with celastrol showed dose-dependent inhibition of fMLPinduced superoxide generation, myeloperoxidase production, and elastase release with half-maximal inhibitory concentration (IC50) values of 5.9 ± 0.1, 1.9 ± 0.2, and 1.5 ± 0.1 µM, respectively. Conclusion: These results indicate that celastrol possesses anti-inflammatory properties via attenuation of fMLP-induced superoxide generation, myeloperoxidase production, and elastase release by peripheral blood neutrophils.

Chemotactic Activity of Collagen-Like Polypeptides for Human Peripheral Blood Neutrophils

1986 ◽  
Vol 39 (3) ◽  
pp. 255-266 ◽  
Author(s):  
Debra L. Laskin ◽  
Terutoshi Kimura ◽  
Shumpei Sakakibara ◽  
David J. Riley ◽  
Richard A. Berg
Keyword(s):  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Chemotactic Activity ◽  
Blood Neutrophils

Activated human peripheral blood neutrophils produce epithelial injury and fibronectin breakdown in vitro

Inflammation ◽  
1992 ◽  
Vol 16 (1) ◽  
pp. 21-30 ◽  
Author(s):  
D. M. Brown ◽  
G. M. Brown ◽  
W. Macnee ◽  
K. Donaldson
Keyword(s):  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Epithelial Injury ◽  
Blood Neutrophils

scholarly journals Production of Macrophage Inflammatory Protein 3α (MIP-3α) (CCL20) and MIP-3β (CCL19) by Human Peripheral Blood Neutrophils in Response to Microbial Pathogens

2003 ◽  
Vol 71 (1) ◽  
pp. 524-526 ◽  
Author(s):  
Tohru Akahoshi ◽  
Takeshi Sasahara ◽  
Rie Namai ◽  
Toshimichi Matsui ◽  
Hiroyuki Watabe ◽  
...  
Keyword(s):  
Immune Responses ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Microbial Pathogens ◽  
T Cell Subsets ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Inflammatory Protein ◽  
Blood Neutrophils ◽  
Macrophage Inflammatory Protein

ABSTRACT Effects of bacterial pathogens on the production of macrophage inflammatory protein 3α (MIP-3α) and MIP-3β from human peripheral blood neutrophils were investigated. Neutrophils produced both chemokines by coincubation with either gram-positive or gram-negative bacteria. Neutrophils may initiate antigen-specific immune responses through the release of these chemokines that are capable of promoting selective recruitment of dendritic cells and T-cell subsets.

Sign in / Sign up

Export Citation Format

Share Document