scholarly journals Molecular Identification and Functional Characterization of a Mitochondrial Sulfonylurea Receptor 2 Splice Variant Generated by Intraexonic Splicing

2009 ◽  
Vol 105 (11) ◽  
pp. 1083-1093 ◽  
Author(s):  
Bin Ye ◽  
Stacie L. Kroboth ◽  
Jie-Lin Pu ◽  
Jason J. Sims ◽  
Nitin T. Aggarwal ◽  
...  
Keyword(s):  
Molecular Identification ◽  
Splice Variant ◽  
Functional Characterization ◽  
Sulfonylurea Receptor ◽  
Sulfonylurea Receptor 2

scholarly journals Molecular Identification and Functional Characterization of the Human Colonic Thiamine Pyrophosphate Transporter

2013 ◽  
Vol 289 (7) ◽  
pp. 4405-4416 ◽  
Author(s):  
Svetlana M. Nabokina ◽  
Katsuhisa Inoue ◽  
Veedamali S. Subramanian ◽  
Judith E. Valle ◽  
Hiroaki Yuasa ◽  
...  
Keyword(s):  
Molecular Identification ◽  
Functional Characterization ◽  
Thiamine Pyrophosphate

Abstract C100: Identification and functional characterization of a novel GLI1 splice variant in breast cancer

2020 ◽  
Author(s):  
Maria S. Dixon ◽  
Lhoucine Chdid ◽  
David R. Lamson ◽  
Michael T. Tarpley ◽  
Helen O. Oladapo ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Splice Variant ◽  
Functional Characterization

scholarly journals Molecular identification and functional characterization of an adenylyl cyclase from the honeybee

2006 ◽  
Vol 96 (6) ◽  
pp. 1580-1590 ◽  
Author(s):  
Sebastian Wachten ◽  
Jana Schlenstedt ◽  
Renate Gauss ◽  
Arnd Baumann
Keyword(s):  
Adenylyl Cyclase ◽  
Molecular Identification ◽  
Functional Characterization

Four novel splice variants of sulfonylurea receptor 1

2002 ◽  
Vol 283 (2) ◽  
pp. C587-C598 ◽  
Author(s):  
Annette Hambrock ◽  
Regina Preisig-Müller ◽  
Ulrich Russ ◽  
Anke Piehl ◽  
Peter J. Hanley ◽  
...  
Keyword(s):  
Fluorescent Protein ◽  
Splice Variants ◽  
Functional Characterization ◽  
Pcr Analysis ◽  
Sulfonylurea Receptor ◽  
Transmembrane Segments ◽  
Sulfonylurea Receptor 1 ◽  
Green Fluorescent ◽  
Splice Forms

ATP-sensitive K+ (KATP) channels are composed of pore-forming Kir6.x subunits and regulatory sulfonylurea receptor (SUR) subunits. SURs are ATP-binding cassette proteins with two nucleotide-binding folds (NBFs) and binding sites for sulfonylureas, like glibenclamide, and for channel openers. Here we report the identification and functional characterization of four novel splice forms of guinea pig SUR1. Three splice forms originate from alternative splicing of the region coding for NBF1 and lack exons 17 (SUR1Δ17), 19 (SUR1Δ19), or both (SUR1Δ17Δ19). The fourth (SUR1C) is a COOH-terminal SUR1-fragment formed by exons 31–39 containing the last two transmembrane segments and the COOH terminus of SUR1. RT-PCR analysis showed that these splice forms are expressed in several tissues with strong expression of SUR1C in cardiomyocytes. Confocal microscopy using enhanced green fluorescent protein-tagged SUR or Kir6.x did not provide any evidence for involvement of these splice forms in the mitochondrial KATP channel. Only SUR1 and SUR1Δ17 showed high-affinity binding of glibenclamide ( K d≈ 2 nM in the presence of 1 mM ATP) and formed functional KATPchannels upon coexpression with Kir6.2.

Sign in / Sign up

Export Citation Format

Share Document