Abstract T P28: Brain MRI FLAIR Signal Intensity Ratio Is Correlated With Matrix Metalloproteinase-9 Level In Acute Ischemic Stroke

Stroke ◽  
2014 ◽  
Vol 45 (suppl_1) ◽  
Author(s):  
Ruchira M Jha ◽  
Thomas W Battey ◽  
Ly Pham ◽  
Hannah Irvine ◽  
Karen Furie ◽  
...  
Keyword(s):  
Matrix Metalloproteinase ◽  
Acute Stroke ◽  
Signal Intensity ◽  
Intensity Ratio ◽  
Brain Mri ◽  
Hemorrhagic Transformation ◽  
Signal Intensity Ratio ◽  
Blood Samples ◽  
The Mean ◽  
Metalloproteinase 9

Introduction: Increased Matrix Metalloproteinase-9 (MMP9) level is associated with symptomatic hemorrhagic transformation (HT) after stroke. Higher risk of HT has also been reported in patients with early FLAIR hyperintensity on brain MRI. Hypothesis: We assessed whether FLAIR hyperintensity is associated with MMP9 levels. Methods: We retrospectively analyzed a cohort of acute stroke subjects who had acute brain MRI images and blood samples within the first 12 hours after onset of stroke. FLAIR hyperintensity was quantitatively assessed using FLAIR signal intensity ratio between the stroke lesion and corresponding normal contralateral hemispheres. For each subject, the FLAIR ratio was generated from the average of 6 regions of interest: 2 white matter and 2 gray matter regions each on 2 separate slices. MMP9 was measured using standard ELISA technique. Univariate and multivariate analyses were used to evaluate the relationship between FR and MMP9 and clinical covariates. Results: 180 subjects had brain MRI and MMP9 available for analysis. MRI occurred within 6 ±3 hours and blood samples were drawn within 7 ± 4 hours from last seen well time. The mean MMP9 level was 238 ± 242 ng/mL and the mean FLAIR ratio was 1.40± 0.23. In univariate analysis, FLAIR ratio was associated with time to MRI (r= 0.18, p=0.01) and MMP9 (r=0.29, p<0.001). In multivariate analysis both FLAIR ratio (p<0.001) and time to MRI (p=0.003) remained associated with MMP9. Conclusions: There is a significant association between FLAIR ratio and MMP9 in acute stroke subjects. This raises the possibility that FLAIR hyperintensity reflects blood brain barrier changes during ischemia. Future studies to validate the prognostic value of FLAIR ratio in predicting symptomatic hemorrhagic transformation are warranted.

Systemic Expression of Matrix Metalloproteinase-9 in Patients With Cerebral Arteriovenous Malformations

Neurosurgery ◽  
2010 ◽  
Vol 66 (2) ◽  
pp. 343-348 ◽  
Author(s):  
Robert M. Starke ◽  
Ricardo J. Komotar ◽  
Brian Y. Hwang ◽  
David K. Hahn ◽  
Marc L. Otten ◽  
...  
Keyword(s):  
Matrix Metalloproteinase ◽  
Arteriovenous Malformations ◽  
Enzyme Linked Immunosorbent Assay ◽  
Healthy Controls ◽  
Cerebral Arteriovenous Malformations ◽  
Blood Samples ◽  
The Mean ◽  
Level 1 ◽  
Metalloproteinase 9

Abstract OBJECTIVE Increased expression angiogenic factors, such as matrix metalloproteinases (MMPs), are associated with the formation of cerebral arteriovenous malformations (AVMs). The objective of this study was to determine plasma levels of MMP-9 of patients with AVMs. METHODS Blood samples were drawn from 15 patients with AVMs before treatment, 24 hours postembolization, 24 hours postresection, and 30 days postresection. Blood samples were also obtained from 30 healthy controls. Plasma MMP-9 concentrations were measured via enzyme-linked immunosorbent assay. RESULTS The mean plasma MMP-9 level in AVM patients at baseline was significantly higher than in control patients: 108.04 ± 16.11 versus 41.44 ± 2.44 ng/mL, respectively. The mean plasma MMP-9 level 1 day after embolization increased to 172.35 ± 53.76 ng/mL, which was not significantly elevated over pretreatment levels. One day after resection, plasma MMP-9 levels increased significantly over pretreatment levels to 230.97 ± 51.00 ng/mL. Mean plasma MMP-9 concentrations 30 days after resection decreased to 92.8 ± 18.7 ng/mL, which was not different from pretreatment levels but was still significantly elevated over control levels. MMP-9 levels did not correlate with patient sex, age, presentation, or AVM size. CONCLUSION Plasma MMP-9 levels are significantly elevated over controls at baseline, increase significantly immediately after surgery, and decrease to pretreatment levels during follow-up.

Comparison of Biomechanical and Anatomical Effects Following Eccentric and Concentric Exertions

2005 ◽  
Vol 49 (14) ◽  
pp. 1287-1291
Author(s):  
Amrish O. Chourasia ◽  
Mary E. Sesto ◽  
Youngkyoo Jung ◽  
Robert S. Howery ◽  
Robert G. Radwin
Keyword(s):  
Signal Intensity ◽  
Intensity Ratio ◽  
Maximum Voluntary Contraction ◽  
Magnetic Resonance Images ◽  
Voluntary Contraction ◽  
Work Place ◽  
Signal Intensity Ratio ◽  
Mechanical Stiffness ◽  
Average Decrease ◽  
Muscle Shortening

Work place exertions may include muscle shortening (concentric) or muscle lengthening (eccentric) contractions. This study investigates the upper limb mechanical properties and magnetic resonance images (MRI) of the involved muscles following submaximal eccentric and concentric exertions. Twelve participants were randomly assigned to perform at 30° per second eccentric or concentric forearm supination exertions at 50% isometric maximum voluntary contraction (MVC) for 30 minutes. Measurement of mechanical stiffness, isometric MVC, localized discomfort and MRI supinator: extensor signal intensity ratio was done before, immediately after, 1 hour after and 24 hours after the bout of exercise. A 53% average decrease in mechanical stiffness after 1 hour was observed for the eccentric group (p< 0.05) compared to a 1% average decrease for the concentric group (p> 0.05). Edema, indicative of swelling, was observed 24 hrs after exercise, with an average increase in the MRI supinator: extensor signal intensity ratio of 36% for the eccentric group and less than 10% for the concentric group (p<0.05).

Association between tPA therapy and raised early matrix metalloproteinase-9 in acute stroke

Neurology ◽  
2006 ◽  
Vol 66 (10) ◽  
pp. 1550-1555 ◽  
Author(s):  
M. Ning ◽  
K. L. Furie ◽  
W. J. Koroshetz ◽  
H. Lee ◽  
M. Barron ◽  
...  
Keyword(s):  
Matrix Metalloproteinase ◽  
Acute Stroke ◽  
Matrix Metalloproteinase 9 ◽  
Metalloproteinase 9

Abstract 3268: Ct Perfusion As A Tool To Predict The Risk Of Hemorrhagic Transformation In Ischemic Stroke Treated With Tissue Plasminogen Activator: A Single Center Experience.

Stroke ◽  
2012 ◽  
Vol 43 (suppl_1) ◽  
Author(s):  
Tareq Kass-Hout ◽  
Maxim Mokin ◽  
Omar Kass-Hout ◽  
Emad Nourollahzadeh ◽  
David Wack ◽  
...  
Keyword(s):  
Ischemic Stroke ◽  
Acute Stroke ◽  
Brain Tissue ◽  
Cerebral Blood Volume ◽  
Ct Perfusion ◽  
Intravenous Thrombolysis ◽  
Hemorrhagic Transformation ◽  
Stroke Therapy ◽  
Two Phase ◽  
The Mean

Objective: To use the Computed Tomography Perfusion (CTP) parameters at the time of hospital admission, including Cerebral Blood Volume (CBV) and Permeability Surface area product (PS), to identify patients with higher risk to develop hemorrhagic transformation in the setting of acute stroke therapy with intravenous thrombolysis. Methods: Retrospective study that compared admission CTP variables between patients with Hemorrhagic Transformation (HT) acute stroke and those with no hemorrhagic transformation. Both groups received standard of care intravenous thrombolysis with tPA. Twenty patients presented to our stroke center between the years 2007 - 2011 within 3 hours after stroke symptoms onset. All patients underwent two-phase 320 slice CTP which creates CBV and PS measurements. Patients were divided into two groups according to whether or not they had HT on a follow up CT head without contrast, done within 36 hours of the thrombolysis therapy. Clinical, demographic and CTP variables were compared between the HT and non-HT groups using logistic regression analyses. Results: HT developed in 8 (40%) patients. Patients with HT had lower ASPECT score ( P =.03), higher NIHSS on admission ( P= .01) and worse outcome ( P= .04) compared to patients who did not develop HT. Baseline blood flow defects were comparable between the two groups. The mean PS for the HT group was 0.53 mL/min/100g brain tissue, which was significantly higher than that for the non-HT group of 0.04 mL/min/100g brain tissue ( P <.0001). The mean area under the curve was 0.92 (95% CI). The PS threshold of 0.26 mL/min/100g brain tissue had a sensitivity of 80% and a specificity of 92% for detecting patients with high risk of hemorrhagic transformation after intravenous thrombolysis. Conclusions: Admission CTP measurements might be useful to predict patients who are at higher risk to develop hemorrhagic transformation after acute ischemic stroke therapy.

Course of matrix metalloproteinase-9 isoforms after the administration of uric acid in patients with acute stroke

2009 ◽  
Vol 256 (4) ◽  
pp. 651-656 ◽  
Author(s):  
Sergio Amaro ◽  
Víctor Obach ◽  
Alvaro Cervera ◽  
Xabier Urra ◽  
Manuel Gómez-Choco ◽  
...  
Keyword(s):  
Uric Acid ◽  
Matrix Metalloproteinase ◽  
Acute Stroke ◽  
Matrix Metalloproteinase 9 ◽  
Metalloproteinase 9

scholarly journals Quantitative Analysis of Apoptotic Cell Death Using Proton Nuclear Magnetic Resonance Spectroscopy

Blood ◽  
1997 ◽  
Vol 89 (10) ◽  
pp. 3778-3786 ◽  
Author(s):  
Francis G. Blankenberg ◽  
Peter D. Katsikis ◽  
Richard W. Storrs ◽  
Christian Beaulieu ◽  
Daniel Spielman ◽  
...  
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Cell Death ◽  
Magnetic Resonance ◽  
1H Nmr ◽  
Signal Intensity ◽  
Intensity Ratio ◽  
Apoptotic Cell ◽  
Proton Nuclear Magnetic Resonance ◽  
Resonance Spectroscopy ◽  
Signal Intensity Ratio

Abstract Quantification of apoptotic cell death in vivo has become an important area of investigation in patients with acute lymphoblastic leukemia (ALL). We have devised a noninvasive analytical method to estimate the percentage of apoptotic lymphoblasts in doxorubicin-treated Jurkat T-cell ALL cultures, using proton nuclear magnetic resonance spectroscopy (1H NMR). We have found that the ratio of the methylene (CH2 ) resonance (at 1.3 ppm) to the methyl (CH3 ) resonance (at 0.9 ppm) signal intensity, as observed by 1H NMR, is directly proportional to the percentage of apoptotic lymphoblasts in vitro. The correlation between the CH2/CH3 signal intensity ratio and the percentage of apoptotic lymphoblasts was optimal 24 to 28 hours after doxorubicin treatment (r2 = .947, N = 27 samples). There was also a direct temporal relationship between an increase in the CH2/CH3 signal intensity ratio and the onset of apoptosis as detected by nuclear morphologic analysis, fluorescein-annexin V flow cytometry, and DNA gel electrophoresis. Thin-layer chromatography confirmed that a dynamic and/or compositional change of the plasma membrane, rather than increases in lipase activity or fatty acid production, appears to account for the increase in the CH2/CH3 signal intensity ratio during apoptosis. 1H NMR may have clinical utility for the early noninvasive assessment of chemotherapeutic efficacy in patients with ALL.

scholarly journals Association of Matrix Metalloproteinase 9 and Cellular Fibronectin and Outcome in Acute Ischemic Stroke: A Systematic Review and Meta-Analysis

2020 ◽  
Vol 11 ◽  
Author(s):  
Lu Wang ◽  
Linghui Deng ◽  
Ruozhen Yuan ◽  
Junfeng Liu ◽  
Yuxiao Li ◽  
...  
Keyword(s):  
Ischemic Stroke ◽  
Matrix Metalloproteinase ◽  
Acute Ischemic Stroke ◽  
Functional Outcome ◽  
Brain Edema ◽  
Meta Analysis ◽  
Hemorrhagic Transformation ◽  
Poor Outcome ◽  
Metalloproteinase 9 ◽  
Cellular Fibronectin

Introduction: The role of matrix metalloproteinase 9 (MMP-9) and cellular fibronectin (c-Fn) in acute ischemic stroke is controversial. We systematically reviewed the literature to investigate the association of circulating MMP-9 and c-Fn levels and MMP-9 rs3918242 polymorphism with the risk of three outcome measures after stroke.Methods: We searched English and Chinese databases to identify eligible studies. Outcomes included severe brain edema, hemorrhagic transformation, and poor outcome (modified Rankin scale score ≥3). We estimated standardized mean differences (SMDs) and pooled odds ratios (ORs) with 95% confidence intervals (CIs).Results: Totally, 28 studies involving 7,239 patients were included in the analysis of circulating MMP-9 and c-Fn levels. Meta-analysis indicated higher levels of MMP-9 in patients with severe brain edema (SMD, 0.76; 95% CI, 0.18–1.35; four studies, 419 patients) and hemorrhagic transformation (SMD, 1.00; 95% CI, 0.41–1.59; 11 studies, 1,709 patients) but not poor outcome (SMD, 0.30; 95% CI, −0.12 to 0.72; four studies, 759 patients). Circulating c-Fn levels were also significantly higher in patients with severe brain edema (SMD, 1.55; 95% CI, 1.18–1.93; four studies, 419 patients), hemorrhagic transformation (SMD, 1.75; 95% CI, 0.72–2.78; four studies, 458 patients), and poor outcome (SMD, 0.46; 95% CI, 0.16–0.76; two studies, 210 patients). Meta-analysis of three studies indicated that the MMP-9 rs3918242 polymorphism may be associated with hemorrhagic transformation susceptibility under the dominant model (TT + CT vs. CC: OR, 0.621; 95% CI, 0.424–0.908; P = 0.014). No studies reported the association between MMP-9 rs3918242 polymorphism and brain edema or functional outcome after acute stroke.Conclusion: Our meta-analysis showed that higher MMP-9 levels were seen in stroke patients with severe brain edema and hemorrhagic transformation but not poor outcome. Circulating c-Fn levels appear to be associated with all three outcomes including severe brain edema, hemorrhagic transformation, and poor functional outcome. The C-to-T transition at the MMP-9 rs3918242 gene appears to reduce the risk of hemorrhagic transformation.

Sign in / Sign up

Export Citation Format

Share Document