Application of DNA Analysis for Identification of Prey Items on Zooplankton: Selective Treatment Method

2021 ◽  
Vol 54 (3) ◽  
pp. 247-256
Author(s):  
Yeon-Ji Chae ◽  
Hye-Ji Oh ◽  
Yong-Jae Kim ◽  
Kwang-Hyeon Chang ◽  
Hyunbin Jo
Keyword(s):  
Dna Analysis ◽  
Treatment Method ◽  
Selective Treatment ◽  
Prey Items

scholarly journals STUDY ON THE EFFECTIVENESS OF INTRACISTERNAL ANTIBIOTIC TREATMENT FOR THE CONTROL OF MASTITIS IN GOATS

2020 ◽  
Vol 23 (1) ◽  
pp. 37-40
Author(s):  
K. Hristov ◽  
R. Pepovich ◽  
B. Nikolov
Keyword(s):  
Post Partum ◽  
Somatic Cells ◽  
Treatment Method ◽  
Subclinical Mastitis ◽  
Control Group ◽  
Dry Period ◽  
Selective Treatment ◽  
Intramammary Infections ◽  
Selective Approach ◽  
The Cost

The aim of this study is to investigate the healing and prophylactic effect of drying-off antibiotic therapy using the selective and non-selective approach of administration of the antibiotics. First experimental group at the start of the dry period were treated non-selectively (all halves) intra-cisternally with antibiotics. In the second group, we only treated those halves that were diagnosed with subclinical mastitis. The control group of 9 goats was not treated. The number of somatic cells in treated groups after birth decreased from 2586 x 10³/ mL to 560 x 10³/mL in the first group and from 1978 x 10³/mL to 526 x 10³/mL in the second. Post partum, 10% of the halves were found to have subclinical mastitis. A healing effect was achieved in 25% (n=5) milk halves. New intramammary infections developed during the dry period also occurred in 5% (n=1) of the milk halves. In the second post-partum study, subclinical mastitis was observed only in 5% (n=1). Healing was achieved in 30% (n = 6) and the new intramammary infections (NIMIs) were 10% (n=2). In the control group, healing was only observed in 5% (n=1) and NIMI were 27.78% (n=5). The application of both treatment approaches, results in reduction in prevalence and manifestation of mastitis, as well as reduction of somatic cells in milk after kidding. The selective treatment method may be preferred, in order to reduce the cost of medications in conducting treatment and prophylaxis programs.

Legionella in sugar factory process waters – Analysis and control in Nordic Sugar

2011 ◽  
pp. 597-600
Author(s):  
Christer Bergwall
Keyword(s):  
Real Time ◽  
Legionella Pneumophila ◽  
Dna Analysis ◽  
Control Strategies ◽  
Cooling Water ◽  
Treatment Method ◽  
Sugar Factory ◽  
Dispersing Agent ◽  
Assay Time ◽  
And Control

This presentation will summarise the experiences gained from a Legionella project within Nordic Sugar which emphasises risk evaluation, modern DNA analysis methods, control strategies and water treatment methods. The project started in 2002 with yearly campaign screenings for Legionella spp and Legionella pneumophila. Initially an ISO Legionella method for quantification was used, but this was replaced with a modern, rapid DNA-based Real Time Polymerase Chain Reaction assay in 2005. The assay time for quantitative results using the Real Time PCR is approximately 3 hours compared to between 10 and 14 days for the ISO-method. All Nordic Sugar factories equipped with cooling towers are screened on a weekly or bi-weekly basis during every sugar campaign. Only one sugar factory has shown consistently positive results for Legionella pneumophila on a moderate level. A multiple biocide approach combined with a dispersing agent was successfully used to control the Legionella. In 2010 a non-chemical treatment method, Wallenius AOT-system was installed to eliminate an annual high cost for biocides. The system successfully controlled the L. pneumophila even when the cooling water was partially exchanged with fresh lake water.

EELS characterization of “Smut” layer films on steel surface prepared for chrome plating by anodic etching

1995 ◽  
Vol 53 ◽  
pp. 538-539
Author(s):  
E Y. Wang ◽  
J. T. Cherian ◽  
A. Madsen ◽  
R. M. Fisher
Keyword(s):  
Steel Surface ◽  
Surface Preparation ◽  
Treatment Method ◽  
Chrome Plating ◽  
Transmission Electron ◽  
Alloy Steels ◽  
Pre Treatment ◽  
Hard Chrome ◽  
Electron Energy Loss

Many steel parts are electro-plated with chromium to protect them against corrosion and to improve their wear-resistance. Good adhesion of the chrome plate to the steel surface, which is essential for long term durability of the part, is extremely dependent on surface preparation prior to plating. Recently, McDonnell Douglas developed a new pre-treatment method for chrome plating in which the steel is anodically etched in a sulfuric acid and hydrofluoric acid solution. On carbon steel surfaces, this anodic pre-treatment produces a dark, loosely adhering material that is commonly called the “smut” layer. On stainless steels and nickel alloys, the surface is only darkened by the anodic pre-treatment and little residue is produced. Anodic pre-treatment prior to hard chrome plating results in much better adherence to both carbon and alloy steels.We have characterized the anodic pre-treated steel surface and the resulting “smut” layer using various techniques including electron spectroscopy for chemical analysis (ESCA) on bulk samples and transmission electron microscopy (TEM) and electron energy-loss spectroscopy (EELS) on stripped films.

Continuous Positive Airway Pressure (CPAP) Therapy for the Treatment of Hypernasality: A Single Case Study

2014 ◽  
Vol 24 (2) ◽  
pp. 48-58 ◽  
Author(s):  
Lakshmi Kollara ◽  
Graham Schenck ◽  
Jamie Perry
Keyword(s):  
Continuous Positive Airway Pressure ◽  
Airway Pressure ◽  
Positive Airway Pressure ◽  
Single Case ◽  
Treatment Method ◽  
Post Treatment ◽  
Subjective Perception ◽  
Cpap Therapy ◽  
Long Term Follow Up

Studies have investigated the applications of Continuous Positive Airway Pressure (CPAP) therapy in the treatment of hypernasality due to velopharyngeal dysfunction (VPD; Cahill et al., 2004; Kuehn, 1991; Kuehn, Moon, & Folkins, 1993; Kuehn et al., 2002). The purpose of this study was to examine the effectiveness of CPAP therapy to reduce hypernasality in a female subject, post-traumatic brain injury (TBI) and pharyngeal flap, who presented with signs of VPD including persistent hypernasality. Improvements in mean velopharyngeal orifice size, subjective perception of hypernasality, and overall intelligibility were observed from the baseline to 8-week post-treatment assessment intervals. Additional long-term assessments completed at 2, 3, and 4 months post-treatment indicated decreases in immediate post-treatment improvements. Results from the present study suggest that CPAP is a safe, non-invasive, and relatively conservative treatment method for reduction of hypernasality in selected patients with TBI. More stringent long-term follow up may indicate the need for repeated CPAP treatment to maintain results.

“Pseudo Homozygous” Activated Protein C Resistance due to Double Heterozygous Factor V Defects (Factor V Leiden Mutation and Type I Quantitative Factor V Defect) Associated with Thrombosis: Report of Two Cases Belonging to Two Unrelated Kindreds

1996 ◽  
Vol 75 (03) ◽  
pp. 422-426 ◽  
Author(s):  
Paolo Simioni ◽  
Alberta Scudeller ◽  
Paolo Radossi ◽  
Sabrina Gavasso ◽  
Bruno Girolami ◽  
...  
Keyword(s):  
Protein C ◽  
Dna Analysis ◽  
Activated Protein C ◽  
Factor V Leiden ◽  
Type I ◽  
Factor V ◽  
Factor V Leiden Mutation ◽  
Thrombotic Risk ◽  
Apc Resistance ◽  
Quantitative Factor

SummaryTwo unrelated patients belonging to two Italian kindreds with a history of thrombotic manifestations were found to have a double heterozygous defect of factor V (F. V), namely type I quantitative F. V defect and F. V Leiden mutation. Although DNA analysis confirmed the presence of a heterozygous F. V Leiden mutation, the measurement of the responsiveness of patients plasma to addition of activated protein C (APC) gave results similar to those found in homozygous defects. It has been recently reported in a preliminary form that the coinheritance of heterozygous F. V Leiden mutation and type I quantitative F. V deficiency in three individuals belonging to the same family resulted in the so-called pseudo homozygous APC resistance with APC sensitivity ratio (APC-SR) typical of homozygous F. V Leiden mutation. In this study we report two new cases of pseudo homozygous APC resistance. Both patients experienced thrombotic manifestations. It is likely that the absence of normal F. V, instead of protecting from thrombotic risk due to heterozygous F. V Leiden mutation, increased the predisposition to thrombosis since the patients became, in fact, pseudo-homozygotes for APC resistance. DNA-analysis is the only way to genotype a patient and is strongly recommended to confirm a diagnosis of homozygous F. V Leiden mutation also in patients with the lowest values of APC-SR. It is to be hoped that no patient gets a diagnosis of homozygous F. V Leiden mutation based on the APC-resi-stance test, especially when the basal clotting tests, i.e., PT and aPTT; are borderline or slightly prolonged.

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