Legionella in sugar factory process waters – Analysis and control in Nordic Sugar
This presentation will summarise the experiences gained from a Legionella project within Nordic Sugar which emphasises risk evaluation, modern DNA analysis methods, control strategies and water treatment methods. The project started in 2002 with yearly campaign screenings for Legionella spp and Legionella pneumophila. Initially an ISO Legionella method for quantification was used, but this was replaced with a modern, rapid DNA-based Real Time Polymerase Chain Reaction assay in 2005. The assay time for quantitative results using the Real Time PCR is approximately 3 hours compared to between 10 and 14 days for the ISO-method. All Nordic Sugar factories equipped with cooling towers are screened on a weekly or bi-weekly basis during every sugar campaign. Only one sugar factory has shown consistently positive results for Legionella pneumophila on a moderate level. A multiple biocide approach combined with a dispersing agent was successfully used to control the Legionella. In 2010 a non-chemical treatment method, Wallenius AOT-system was installed to eliminate an annual high cost for biocides. The system successfully controlled the L. pneumophila even when the cooling water was partially exchanged with fresh lake water.