Bursaphelenchus tadamiensis n. sp. (Nematoda: Aphelenchoididae), isolated from a stag beetle, Dorcus striatipennis (Coleoptera: Lucanidae), from Japan

Bursaphelenchus tadamiensis n. sp. is described. The new species was isolated during a field survey of nematodes associated with stag beetles. It was recovered from the dissected body of a Dorcus striatipennis, from Tadami, Fukushima, Japan. The new species is medium sized for the genus, with males 486-772 μm and females 643-997 μm in body length, and has four lateral lines, six male genital papillae (P1 ventral single papilla is missing or vestigial), mitten-shaped spicule with clear dorsal and ventral limbs, rather large squared bursal flap, vulva lacking flap, female tail long, tapering and ventrally curved. Based upon its diagnostic morphological characters, the new species belongs to the B. fungivorus group and is close to B. thailandae, B. willibaldi and B. braaschae. It is distinguished from these three species by morphology of the male bursal flap and several morphometric values, e.g., the V value of B. tadamiensis n. sp. is clearly larger than that of the above three species. The molecular phylogenetic analyses inferred from near full-length SSU and D2/D3 LSU supported the morphological observations, i.e., the new species is molecularly similar to B. thailandae, B. willibaldi, B. braaschae and B. kiyoharai. The stag beetle association of the new species may be an accidental phoresy, although the nematode could be undergoing carrier (vector) switching, because only two adult (no dauer juveniles) individuals were isolated from only one of 100 beetles examined. The insect organ with which the nematode was associated is unknown.

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Bursaphelenchus firmae n. sp. (Nematoda: Aphelenchoididae), isolated from Monochamus grandis Waterhouse that emerged from dead firs, Abies firma Sieb. et Zucc.

Nematology ◽

10.1163/156854111x602974 ◽

2012 ◽

Vol 14 (4) ◽

pp. 395-404 ◽

Cited By ~ 22

Author(s):

Natsumi Kanzaki ◽

Noritoshi Maehara ◽

Takuya Aikawa ◽

Kazuma Matsumoto

Keyword(s):

New Species ◽

Phylogenetic Analyses ◽

Morphological Characters ◽

Lateral View ◽

The Body ◽

Tracheal System ◽

Abies Firma ◽

Lateral Lines ◽

Pine Trees ◽

Molecular Phylogenetic

Bursaphelenchus firmae n. sp. is described. This new species was isolated during a field survey of longhorn beetle-associated nematodes. The fourth-stage dispersal (dauer) juveniles of the new species were recovered from dissected bodies (tracheal system) of Monochamus grandis, which emerged from dead logs of Japanese fir, Abies firma, collected from Hachioji, Tokyo, Japan. The new species is mid-sized for the genus, with females 603-828 μm and males 530-698 μm long. Four lateral lines occur on the body surface and seven genital papillae are found in males (P1 ventral single papilla and P2-P4 pairs). A long and arcuate spicule forms a trapezium in lateral view and a rather large, sub-squared, bursal flap and a vulva with conspicuous vulval flap are present. The female tail is smoothly tapered and possesses a conspicuous and blunt mucro. Based upon its diagnostic morphological characters, the new species belongs to the B. xylophilus group of the genus, and is closely related to B. fraudulentus, B. mucronatus, B. doui, B. macromucronatus and B. populi. It is distinguished from these five species by the morphology of the male bursal flap and the female mucro and several morphometric values. Molecular phylogenetic analyses inferred from D2/D3 LSU suggest that the new species is close to B. mucronatus and B. xylophilus, i.e., these three species form a well supported monophyletic clade within the genus. Although the new species has a weak pathogenicity to pine trees, it does not seem to be a severe risk to native pine forests.

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Bursaphelenchus kesiyae n. sp. (Nematoda: Aphelenchoididae), isolated from dead wood of Pinus kesiya Royle ex Gordon (Pinaceae) from Vietnam, with proposal of new subgroups in the B. fungivorus group

Nematology ◽

10.1163/15685411-00002949 ◽

2016 ◽

Vol 18 (2) ◽

pp. 133-146 ◽

Cited By ~ 4

Author(s):

Natsumi Kanzaki ◽

Takuya Aikawa ◽

Noritoshi Maehara ◽

Pham Quang Thu

Keyword(s):

New Species ◽

Dead Wood ◽

Morphological Characters ◽

Pinus Kesiya ◽

Lateral Lines ◽

Pine Trees ◽

Molecular Phylogenetic ◽

Cryptic Species Complex ◽

Close Relatives ◽

Male Genital

Bursaphelenchus kesiyae n. sp. is described. The new species was isolated from dead wood of Pinus kesiya during a field survey of nematodes associated with dead pine trees (Pinus spp.). The new species is medium- to large-sized for the genus, with males 690-1059 μm and females 837-1122 μm in body length, and has four lateral lines, six male genital papillae (P1 ventral single papilla is missing or vestigial), a mitten-shaped spicule with clear dorsal and ventral limbs, an indistinctive small and narrow bursal flap, vulva lacking any flap apparatus, and female tail long, tapering and straight or slightly ventrally curved. Based upon its diagnostic morphological characters, the new species belongs to the B. fungivorus group and is closely related to B. thailandae and B. parathailandae, with which it forms a cryptic species complex. However, the new species is distinguished from these two species by the morphology of the male bursal flap and several morphometric values, i.e., the bursal flap of the new species is inconspicuous, or almost lost in many individuals. Molecular phylogenetic analysis inferred from near-full-length SSU and D2-D3 LSU supported the morphological observations, i.e., the new species is molecularly similar to B. thailandae and B. parathailandae, but could be distinguished phylogenetically. Further, differences in molecular sequences in SSU and D2-D3 LSU between the new species and its close relatives are slightly higher than those between B. xylophilus and B. mucronatus, thus, identification of the species status for B. kesiyae n. sp. is considered warranted.

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Bursaphelenchus rufipennis n. sp. (Nematoda: Parasitaphelenchinae) and redescription of Ektaphelenchus obtusus (Nematoda: Ektaphelenchinae), associates from nematangia on the hind wings of Dendroctonus rufipennis (Coleoptera: Scolytidae)

Nematology ◽

10.1163/156854108786161517 ◽

2008 ◽

Vol 10 (6) ◽

pp. 925-955 ◽

Cited By ~ 16

Author(s):

Natsumi Kanzaki ◽

Robin M. Giblin-Davis ◽

Yasmin J. Cardoza ◽

Weimin Ye ◽

Kenneth F. Raffa ◽

...

Keyword(s):

New Species ◽

Morphological Characters ◽

Morphological Examination ◽

Dendroctonus Rufipennis ◽

Lateral Field ◽

The Past ◽

Female Tail ◽

Molecular Phylogenetic ◽

Voucher Specimens ◽

Tail Tip

Abstract Two species of aphelench, Bursaphelenchus rufipennis n. sp. and Ektaphelenchus obtusus, were isolated from the 'nematangia', cocoon-like structures found at the base of the hind wings of Dendroctonus rufipennis. The nematangia contained adult females of E. obtusus and the dauer juveniles of B. rufipennis n. sp. Only B. rufipennis n. sp. could be cultured on Monilinia fructicola on LGPDA (lactic acid-treated, glycerol-supplemented, potato dextrose agar). The new species of Bursaphelenchus is described and figured and some additional morphological characters are ascribed to E. obtusus, E. josephi, E. sandiaensis, E. smaelus (= E. prolobos) and E. terebranus after examination of type and/or voucher specimens. Bursaphelenchus rufipennis n. sp. has an adult body length of ca 500-1000 μm, medium a ratios (ca 25-38 for females and ca 30-40 for males), b ratios of ca 8-13 (female) and 7-11 (male), c ratios of ca 15-22 (female and male), c′ ratios of ca 3-4 (female) and ca 2-3 (male), and is characterised by three incisures in the lateral field, mitten-shaped spicules and a conical female tail that curves ventrally and possesses a variable tail tip. The new species is morphologically closest to B. corneolus, B. curvicaudatus, B. gerberae, B. paracorneolus and B. talonus. Morphological examination of type and/or voucher specimens of five Ektaphelenchus species revealed coarse transverse body annulation and three pairs of male caudal papillae (except for the two species where males are not described). Clear typological differences were observed among these five Ektaphelenchus species in the structure of the lip region, presence/absence of stylet knobs and male spicule morphology. Although these characters have not been consistently documented in the past, they may be diagnostic for species in the genus. Molecular phylogenetic analysis based on SSU and D2/D3 LSU sequences revealed that B. rufipennis n. sp. was closest to B. paracorneolus and that E. obtusus was closest to species of Ektaphelenchoides and a Cryptaphelenchus sp.

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A curious nematode, Diploscapteroides persicus n. sp. (Nematoda: Rhabditida: Rhabditomorpha), from Iran with a note on the status of the male genital papillae

Nematology ◽

10.1163/15685411-00002922 ◽

2015 ◽

Vol 17 (9) ◽

pp. 1045-1056 ◽

Cited By ~ 1

Author(s):

Razieh Ghaemi ◽

Ebrahim Pourjam ◽

Sergio Álvarez-Ortega ◽

Majid Pedram ◽

Mohammad Reza Atighi

Keyword(s):

New Species ◽

Phylogenetic Analyses ◽

Tail Length ◽

Molecular Data ◽

Rrna Genes ◽

Valid Species ◽

Female Tail ◽

The Status ◽

Species Being ◽

Male Genital

Diploscapteroides persicus n. sp. is described and illustrated based on morphological, morphometric and molecular data. The new species is mainly characterised by female body length of 469-673 μm, female tail length of 51-99 μm, shape of gubernaculum and spicules and arrangement of male genital papillae. Also, the arrangement of male genital papillae of the new species shows the presence of precloacal papillae, a feature that has not been previously described for the genus. The new species is comparable with all valid species of the genus, namely D. boettgeri, D. brevicauda, D. chitinolabiatus, D. coroniger, D. dacchensis and D. flexuosus, from which the morphological comparisons of the new species with aforementioned species are discussed. Phylogenetic analyses using partial sequences of SSU and LSU rRNA genes were performed for the new species, being the first representative of the genus to be phylogenetically studied using both Bayesian inference (BI) and maximum likelihood (ML) methods, and revealed that D. persicus n. sp. formed a clade with a species of Cephaloboides.

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Leptolejeunea nigra (Lejeuneaceae), a new species with brownish black ocelli based upon morphology and DNA sequences

Phytotaxa ◽

10.11646/phytotaxa.427.1.4 ◽

2019 ◽

Vol 427 (1) ◽

pp. 31-42

Author(s):

LEI SHU ◽

RUI-LIANG ZHU

Keyword(s):

New Species ◽

Molecular Phylogeny ◽

Dna Sequences ◽

Phylogenetic Analyses ◽

Morphological Characters ◽

Time Span ◽

Molecular Dating ◽

Molecular Phylogenetic ◽

Brownish Black ◽

A New Species

Based on molecular phylogenetic analyses and morphological characters, a new species from Bangladesh, northern Vietnam, and southwestern China, Leptolejeunea nigra, is described. It is mostly similar to L. balansae but remarkable for having brownish black ocelli in its leaf lobes. In the molecular phylogeny, the samples of L. nigra are not nested within any clade and form an independent lineage. In particular, the molecular dating suggested that the divergence of L. nigra happened in time span of the formation of the Himalayas.

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Molecular and morphological characterisation of Laimaphelenchus spiflatus n. sp. (Nematoda: Aphelenchoididae) from China

Nematology ◽

10.1163/15685411-00003344 ◽

2020 ◽

Vol 22 (8) ◽

pp. 843-853

Author(s):

Jianfeng Gu ◽

Munawar Maria ◽

Yiwu Fang ◽

Xianfeng Chen ◽

Lele Liu

Keyword(s):

New Species ◽

Body Size ◽

Phylogenetic Analyses ◽

Morphological Characters ◽

The Other ◽

Morphometric Data ◽

Lateral Field ◽

Chinese Pine ◽

Lateral Lines ◽

Genomic Regions

Summary Laimaphelenchus spiflatus n. sp. isolated from declining Chinese pine, Pinus tabuliformis, is described and illustrated. The new species can be characterised by its relatively long body size of 1150 ± 108 (976-1437) μm for females and 1092 ± 78.6 (905-1235) μm for males, lateral field with four lines, females with a long vulval flap, and tail conoid, slightly ventrally curved and ending in a stalk having 8-12 projections when observed with SEM. Males are common, with 27.3 (23.4-28.8) μm long spicules having blunt (not well-developed) condylus and rostrum and truncate simple distal tip, and four caudal papillae. By having a vulval flap, four lateral lines and tail end with a stalk (without four tubercles), the new species comes close to L. preissii, L. simlaensis, and L. unituberculus, but can be differentiated from them by morphological characters and morphometric data. In phylogenetic analyses using near full length 18S and D2-D3 expansion segments of 28S rDNA, Laimaphelenchus spiflatus n. sp. appeared as an independent lineage separated from the other Laimaphelenchus spp. that are currently sequenced for their aforementioned genomic regions.

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A revision of dragon millipedes I: genus Desmoxytes Chamberlin, 1923, with the description of eight new species (Diplopoda, Polydesmida, Paradoxosomatidae)

ZooKeys ◽

10.3897/zookeys.761.24214 ◽

2018 ◽

Vol 761 ◽

pp. 1-177 ◽

Cited By ~ 9

Author(s):

Ruttapon Srisonchai ◽

Henrik Enghoff ◽

Natdanai Likhitrakarn ◽

Somsak Panha

Keyword(s):

New Species ◽

Southeast Asia ◽

Present Article ◽

Phylogenetic Analyses ◽

Morphological Characters ◽

New Genera ◽

Distribution Maps ◽

New Synonyms ◽

Molecular Phylogenetic ◽

First Time

The dragon millipede genusDesmoxytess.l. is split into five genera, based on morphological characters and preliminary molecular phylogenetic analyses. The present article includes a review ofDesmoxytess.s., while future articles will deal withHylomusCook and Loomis, 1924 and three new genera which preliminarily are referred to as the ‘acantherpestes’, ‘gigas’, and ‘spiny’ groups. Diagnostic morphological characters of each group are discussed.Hylomusis resurrected as a valid genus and the following 33 species are assigned to it:H.asper(Attems, 1937),comb. n.,H.cattienensis(Nguyen, Golovatch & Anichkin, 2005),comb. n.,H.cervarius(Attems, 1953),comb. n.,H.cornutus(Zhang & Li, 1982),comb. n.,H.dracoCook & Loomis, 1924,stat. rev.,H.enghoffi(Nguyen, Golovatch & Anichkin, 2005),comb. n.,H.eupterygotus(Golovatch, Li, Liu & Geoffroy, 2012),comb. n.,H.getuhensis(Liu, Golovatch & Tian, 2014),comb. n.,H.grandis(Golovatch, VandenSpiegel & Semenyuk, 2016),comb. n.,H.hostilis(Golovatch & Enghoff, 1994),comb. n.,H.jeekeli(Golovatch & Enghoff, 1994),comb. n.,H.lingulatus(Liu, Golovatch & Tian, 2014),comb. n.,H.laticollis(Liu, Golovatch & Tian, 2016),comb. n.,H.longispinus(Loksa, 1960),comb. n.,H.lui(Golovatch, Li, Liu & Geoffroy, 2012),comb. n.,H.minutuberculus(Zhang, 1986),comb. n.,H.nodulosus(Liu, Golovatch & Tian, 2014),comb. n.,H.parvulus(Liu, Golovatch & Tian, 2014),comb. n.,H.phasmoides(Liu, Golovatch & Tian, 2016),comb. n.,H.pilosus(Attems, 1937),comb. n.,H.proximus(Nguyen, Golovatch & Anichkin, 2005),comb. n.,H.rhinoceros(Likhitrakarn, Golovatch & Panha, 2015),comb. n.,H.rhinoparvus(Likhitrakarn, Golovatch & Panha, 2015),comb. n.,H.scolopendroides(Golovatch, Geoffroy & Mauriès, 2010),comb. n.,H.scutigeroides(Golovatch, Geoffroy & Mauriès, 2010),comb. n.,H.similis(Liu, Golovatch & Tian, 2016),comb. n.,H.simplex(Golovatch, VandenSpiegel & Semenyuk, 2016),comb. n.,H.simplipodus(Liu, Golovatch & Tian, 2016),comb. n.,H.specialis(Nguyen, Golovatch & Anichkin, 2005),comb. n.,H.spectabilis(Attems, 1937),comb. n.,H.spinitergus(Liu, Golovatch & Tian, 2016),comb. n.,H.spinissimus(Golovatch, Li, Liu & Geoffroy, 2012),comb. n.andH.variabilis(Liu, Golovatch & Tian, 2016),comb. n.Desmoxytess.s. includes the following species:D.breviverpaSrisonchai, Enghoff & Panha, 2016;D.cervina(Pocock,1895);D.delfae(Jeekel, 1964);D.desSrisonchai, Enghoff & Panha, 2016;D.pinnasqualiSrisonchai, Enghoff & Panha, 2016;D.planata(Pocock, 1895);D.purpuroseaEnghoff, Sutcharit & Panha, 2007;D.takensisSrisonchai, Enghoff & Panha, 2016;D.taurina(Pocock, 1895);D.terae(Jeekel, 1964), all of which are re-described based mainly on type material. Two new synonyms are proposed:DesmoxytespterygotaGolovatch & Enghoff, 1994,syn. n.(=Desmoxytescervina(Pocock, 1895)),DesmoxytesrubraGolovatch & Enghoff, 1994,syn. n.(=Desmoxytesdelfae(Jeekel, 1964)). Six new species are described from Thailand:D.aurataSrisonchai, Enghoff & Panha,sp. n.,D.corythosaurusSrisonchai, Enghoff & Panha,sp. n.,D.eurosSrisonchai, Enghoff & Panha,sp. n.,D.flabellaSrisonchai, Enghoff & Panha,sp. n.,D.golovatchiSrisonchai, Enghoff & Panha,sp. n.,D.octoconigeraSrisonchai, Enghoff & Panha,sp. n., as well as one from Malaysia:D.perakensisSrisonchai, Enghoff & Panha,sp. n., and one from Myanmar:D.waepyanensisSrisonchai, Enghoff & Panha,sp. n.The species can mostly be easily distinguished by gonopod structure in combination with other external characters; some cases of particularly similar congeners are discussed. All species ofDesmoxytess.s. seem to be endemic to continental Southeast Asia (except the ‘tramp’ speciesD.planata). Some biological observations (relationship with mites, moulting) are recorded for the first time. Complete illustrations of external morphological characters, an identification key, and distribution maps of all species are provided.

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Morphometric and phylogenetic analyses of Serpentirhabdias viperidicus n. sp. (Nematoda: Rhabdiasidae) from the lancehead snake Bothrops moojeni Hoge, 1966 (Reptilia: Serpentes: Viperidae) in Brazil

Journal of Helminthology ◽

10.1017/s0022149x16000377 ◽

2016 ◽

Vol 91 (3) ◽

pp. 360-370 ◽

Cited By ~ 6

Author(s):

D.H. Morais ◽

A. Aguiar ◽

M.I. Müller ◽

R.B. Narciso ◽

L.A.F. da Silva ◽

...

Keyword(s):

New Species ◽

Phylogenetic Analyses ◽

Morphological Characters ◽

Neotropical Region ◽

South American ◽

Paulo State ◽

Molecular Approaches ◽

Molecular Phylogenetic ◽

Bothrops Moojeni ◽

A New Species

AbstractSerpentirhabdias viperidicus n. sp. (Nematoda: Rhabdiasidae) is described from the lungs of the ‘Brazilian lancehead’ Bothrops moojeni (Hoge, 1966) from the savannah in São Paulo State, Brazil. The new species is the eighth species of Serpentirhabdias described in the Neotropical region, and differs from other species mainly by a combination of characters: lips slightly notable, presence of fine striations at posterior ends, presence of two parallel lines with intercalated pores, a pore-shaped phasmid situated at the level of the anal aperture and another two in the posterior half of the tail. It is the first species of Serpentirhabdias reported in this snake host and the second species of this genus found parasitizing South American viperidian snakes. Molecular phylogenetic analysis using ribosomal (ITS and 28S partial) genes confirms Serpentirhabdias viperidicus n. sp. as a new species that clustered in the Serpentirhabdias clade, sister taxon to Serpentirhabdias fuscovenosa and Serpentirhabdias elaphe. This is the first description of Serpentirhabdias species from Brazil using molecular approaches and morphological characters to confirm the monophyly of this recent genus.

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Melanoleuca kashmirensis sp. nov. in subg. Urticocystis from Pakistan

Phytotaxa ◽

10.11646/phytotaxa.434.1.6 ◽

2020 ◽

Vol 434 (1) ◽

pp. 89-100

Author(s):

ZIA ULLAH ◽

RUBAB KHURSHED ◽

MUHAMMAD BINYAMIN KHAN ◽

ISRAR AHMAD ◽

SANA JABEEN ◽

...

Keyword(s):

New Species ◽

Phylogenetic Analyses ◽

Morphological Characters ◽

Morphological Features ◽

New Taxon ◽

Line Drawings ◽

Phylogenetic Data ◽

Molecular Phylogenetic ◽

A New Species ◽

Molecular Phylogenetic Data

During this investigation, a new species of Melanoleuca subg. Urticocystis with urticiform cheilocystidia is proposed. The identification is based on morphological characters and molecular phylogenetic analyses using ITS and 28S sequences. A comparison of the morphological features and molecular phylogenetic data with the taxa of subg. Urticocystis confirms the identity of the species as a new taxon. The detailed descriptions, line drawings and phylogenetic results are given.

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Pseudaphelenchus vindai n. sp. (Tylenchomorpha: Aphelenchoididae) associated with termites (Termitidae) in Barro Colorado Island, Panama

Nematology ◽

10.1163/138855410x496187 ◽

2010 ◽

Vol 12 (6) ◽

pp. 905-914 ◽

Cited By ~ 14

Author(s):

Natsumi Kanzaki ◽

Robin M. Giblin-Davis ◽

E. Allen Herre ◽

Rudolf H. Scheffrahn ◽

Barbara J. Center

Keyword(s):

New Species ◽

Field Survey ◽

Barro Colorado Island ◽

Subterranean Termites ◽

Lateral Field ◽

Female Tail ◽

The Family ◽

Molecular Phylogenetic ◽

Pharyngeal Gland ◽

Basal Position

Abstract In 2008, a field survey of termite-associated nematodes was conducted on Barro Colorado Island, Smithsonian Tropical Research Institute (BCI, STRI), Panama. During that survey, an undescribed Pseudaphelenchus species was isolated from three species of subterranean termites, Amitermes beaumonti, Microcerotermes exiguous and Obtusitermes panamae. The nematode is described and figured herein as P. vindai n. sp. The new species is morphologically similar to its only congener, P. yukiae, i. e., these two species share a thin stylet with small and clear basal knobs, a true bursa supported by three bursal limb-like genital papillae and a nerve ring surrounding the anterior clear region of the pharyngeal gland lobe and intestine. The molecular phylogenetic analysis based upon near full length (ca 1.7 kb) SSU ribosomal DNA sequence suggested that the new species forms a well supported clade with P. yukiae, at the basal position of the family Aphelenchoididae. The new species is distinguished from P. yukiae by possessing a clear condylus and rostrum of the capitulum and arcuate calomus/lamina complex of the spicules vs no condylus and rostrum and a relatively straight calomus/lamina complex, long and tapering female tail without small mucro vs blunt with small mucro present and possession of lateral field with three incisures vs four incisures.

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