Mitochondrial DNA differences distinguishing Meloidogyne mayaguensis from the major species of tropical root-knot nematodes

Abstract- The mitochondrial DNA region between the COII and lRNA genes and the 63 base pair tandem repeat region have been used to differentiate and characterise Meloidogyne spp. In this study these regions have been amplified from M. mayaguensis, M. javanica, M. arenaria, M. incognita and M. hapla. Meloidogyne mayaguensis produces a unique product of 705 bp from the COII and lRNA region. Also, a product of 322 bp was produced from the 63 bp repeat region of M. mayaguensis unlike M. javanica, M. arenaria, and M. incognita that exhibit hypervariability in this region. Meloidogyne mayaguensis is a widely distributed root-knot nematode with the potential to cause great economic damage. These molecular diagnostics can be used for accurate identification of M. mayaguensis and can be used to monitor the occurrence and spread of this species, and to provide quarantine services tools to limit its dispersal.

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Diagnostic methods for identification of root-knot nematodes species from Brazil

Ciência Rural ◽

10.1590/0103-8478cr20170449 ◽

2018 ◽

Vol 48 (2) ◽

Cited By ~ 5

Author(s):

Tiago Garcia da Cunha ◽

Liliane Evangelista Visôtto ◽

Everaldo Antônio Lopes ◽

Claúdio Marcelo Gonçalves Oliveira ◽

Pedro Ivo Vieira Good God

Keyword(s):

Dna Analysis ◽

Management Strategies ◽

Molecular Techniques ◽

Diagnostic Methods ◽

Root Knot Nematode ◽

Accurate Identification ◽

Root Knot Nematodes ◽

Major Species ◽

Meloidogyne Spp ◽

Esterase Phenotypes

ABSTRACT: The accurate identification of root-knot nematode (RKN) species (Meloidogyne spp.) is essential for implementing management strategies. Methods based on the morphology of adults, isozymes phenotypes and DNA analysis can be used for the diagnosis of RKN. Traditionally, RKN species are identified by the analysis of the perineal patterns and esterase phenotypes. For both procedures, mature females are required. Over the last few decades, accurate and rapid molecular techniques have been validated for RKN diagnosis, including eggs, juveniles and adults as DNA sources. Here, we emphasized the methods used for diagnosis of RKN, including emerging molecular techniques, focusing on the major species reported in Brazil.

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OC125, M11 and OV197 Epitopes Are Not Uniformly Distributed in the Tandem-Repeat Region of CA125 and Require the Entire SEA Domain

Disease Markers ◽

10.1155/2013/917898 ◽

2013 ◽

Vol 34 (4) ◽

pp. 257-267 ◽

Cited By ~ 15

Author(s):

Alessandro Bressan ◽

Francesca Bozzo ◽

Carlo Alberto Maggi ◽

Monica Binaschi

Keyword(s):

Ovarian Cancer ◽

Monoclonal Antibodies ◽

Western Blot ◽

Tandem Repeat ◽

Blot Analysis ◽

Western Blot Analysis ◽

Human Cancer ◽

Ovarian Cancer Cells ◽

Repeat Region ◽

Tandem Repeat Region

The human cancer antigen 125 (CA125) is over-expressed in epithelial ovarian cancer cells and it plays a role in the pathogenesis of ovarian cancer. This protein presents a repeat region containing up to sixty tandem repeat units. The anti-CA125 monoclonal antibodies have been previously classified into three groups: two major families, the OC125-like antibodies and M11-like antibodies, and a third group, the OV197-like antibodies. A model in which a single repeat unit contains all the epitopes for these antibodies has been also proposed, even if their exact position is still undetermined. In the present work, the affinities of the monoclonal antibodies, representative of the three families, have been investigated for different CA125-recombinant repeats through Western blot analysis. Different patterns of antibody recognition for the recombinant repeats show that CA125 epitopes are not uniformly distributed in the tandem repeat region of the protein. The minimal region for the recognition of these antibodies has been also individuated in the SEA domain through the subcloning of deleted sequences of the highly recognized repeat-25 (R-25), their expression as recombinant fragments inE. coliand Western blot analysis. Obtained data have been further confirmed by ELISA using the entire R-25 as coating antigen.

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