Levipalatum texanum n. gen., n. sp. (Nematoda: Diplogastridae), an androdioecious species from the south-eastern USA

Nematology ◽  
2014 ◽  
Vol 16 (6) ◽  
pp. 695-709 ◽  
Author(s):  
Erik J. Ragsdale ◽  
Erik J. Ragsdale ◽  
Natsumi Kanzaki ◽  
Erik J. Ragsdale ◽  
Natsumi Kanzaki ◽  
...  
Keyword(s):  
New Species ◽  
Model Organism ◽  
Sister Group ◽  
Small Subunit ◽  
Phylogenetic Position ◽  
Rrna Gene ◽  
Small Subunit Rrna ◽  
Protein Coding ◽  
Eastern Usa ◽  
A New Species

A new species of diplogastrid nematode, Levipalatum texanum n. gen., n. sp., was isolated from scarab beetles (Coleoptera: Scarabaeidae) in Texas and baited from soil in Virginia, USA. Levipalatum n. gen. is circumscribed by stomatal and pharyngeal morphology, namely a long, hooked dorsal tooth connected to a ‘palate’ projecting anteriad and mediad, subventral telostegostomatal ridges of denticles, and the dorsal radius of the pharynx bulging anteriad. The males of the new species are distinguished from most other Diplogastridae by the frequent presence of ten pairs of genital papillae. Phylogenetic relationships inferred from 11 ribosomal protein-coding genes and a fragment of the small subunit rRNA gene strongly support L. texanum n. gen., n. sp. to be a sister group to Rhabditolaimus, which lacks all the stegostomatal and pharyngeal characters diagnosing the new genus. The new species expands comparative studies of the radiation of feeding morphology that are anchored on the model organism Pristionchus pacificus. The phylogenetic position of L. texanum n. gen., n. sp. indicates a new case of convergent evolution of hermaphroditism in Diplogastridae.

Description of Tetrahymena empidokyrea n.sp., a new species in the Tetrahymena pyriformis sibling species complex (Ciliophora, Oligohymenophorea), and an assessment of its phylogenetic position using small-subunit rRNA sequences

1996 ◽  
Vol 74 (10) ◽  
pp. 1898-1906 ◽  
Author(s):  
Cheryl A. Jerome ◽  
Denis H. Lynn ◽  
Ellen M. Simon
Keyword(s):  
New Species ◽  
Sibling Species ◽  
Species Complex ◽  
Tetrahymena Pyriformis ◽  
Small Subunit ◽  
Biological Species ◽  
Phylogenetic Position ◽  
Small Subunit Rrna ◽  
A New Species ◽  
Rrna Sequences

Ciliates infected 0.25% of adult Aedes sp. mosquitoes collected in Guelph, Ontario. Morphological observations of live and stained specimens indicated that these ciliates belonged to the Tetrahymena pyriformis sibling species complex. This study provides the first well-documented case of insects being infected by a species in the T. pyriformis complex. Mating experiments demonstrated that these "mosquito" ciliates were reproductively isolated from previously described biological species in the complex, and are designated a new species, Tetrahymena empidokyrea n.sp. Phylogenetic analysis using SSrDNA sequences suggested that T. empidokyrea n.sp. is either basal to all species in the genus Tetrahymena or basal to one of the two main lineages in that genus.

scholarly journals New Light on Historical Specimens Reveals a New Species of Ladybird (Coleoptera: Coccinellidae): Morphological, Museomic, and Phylogenetic Analyses

Insects ◽  
2020 ◽  
Vol 11 (11) ◽  
pp. 766
Author(s):  
Karen Salazar ◽  
Romain Nattier
Keyword(s):  
New Species ◽  
Biological Diversity ◽  
Phylogenetic Analyses ◽  
Sister Group ◽  
Molecular Data ◽  
Phylogenetic Position ◽  
Protein Coding ◽  
Total Dna ◽  
Next Generation Sequencing Ngs ◽  
A New Species

Natural history collections house an important source of genetic data from yet unexplored biological diversity. Molecular data from museum specimens remain underexploited, which is mainly due to the degradation of DNA from specimens over time. However, Next-Generation Sequencing (NGS) technology can now be used to sequence “old” specimens. Indeed, many of these specimens are unique samples of nomenclatural types and can be crucial for resolving systematic or biogeographic scientific questions. Two ladybird beetle specimens from Patagonia corresponding to a new species of the genus Eriopis Mulsant were found in the collections of the Muséum national d’Histoire naturelle (MNHN), Paris. Here, we describe Eriopis patagonia Salazar, sp. nov. Total DNA of one of the two specimens was sequenced by NGS using a paired-end Illumina approach. We reconstruct and characterize the mitochondrial genome of this species (16,194 bp). Then, the protein-coding genes (PCGs) and ribosomal RNAs (rRNAs) were used to infer by maximum likelihood and Bayesian Inference the phylogenetic position of E. patagonia among 27 representatives of Coccinellidae. Phylogenetic analysis confirmed the position of Eriopis as sister group to Cycloneda Crotch. Hence, we highlight the high potential of sequencing technology for extracting molecular information from old specimens, which are used here for the systematic study of a genus, while demonstrating the importance of preserving biological collections.

scholarly journals Two new marine ciliates, Euplotes sinicus sp. nov. and Euplotes parabalteatus sp. nov., and a new small subunit rRNA gene sequence of Euplotes rariseta (Ciliophora, Spirotrichea, Euplotida)

2010 ◽  
Vol 60 (5) ◽  
pp. 1241-1251 ◽  
Author(s):  
Jiamei Jiang ◽  
Qianqian Zhang ◽  
Xiaozhong Hu ◽  
Chen Shao ◽  
Khaled A. S. Al-Rasheid ◽  
...  
Keyword(s):  
Phylogenetic Trees ◽  
Sister Group ◽  
Small Subunit ◽  
Phylogenetic Position ◽  
Rrna Gene ◽  
Ssu Rrna ◽  
The Novel ◽  
Small Subunit Rrna ◽  
Small Subunit Rrna Gene ◽  
Phylogenetic Lineages

The morphology, infraciliature and silverline system of two marine Euplotes, Euplotes sinicus sp. nov. and Euplotes parabalteatus sp. nov., isolated from seawater near Qingdao, China, were investigated. E. sinicus is characterized by having conspicuous dorsal ridges, a single marginal cirrus and a silverline system of the double-patella-I type. E. parabalteatus is an extremely small form (only about 35 μm long) with 6–7 dorsal kineties and a silverline system of the double-eurystomus type. Small subunit (SSU) rRNA-based phylogenetic trees were constructed with three different methods and these firmly demonstrated that the novel species represent two distinct phylogenetic lineages within the genus Euplotes, branching as a sister group to all other sequenced congeners. In addition, the SSU rRNA gene of another rare, morphologically similar form, Euplotes rariseta, was sequenced. This revealed the phylogenetic position of E. rariseta to be basal to one of the major groups of Euplotes rather than close to Euplotes nobilii.

scholarly journals Ixodes kangdingensis (Acari: Ixodidae), a new species from the Siberian weasel, Mustela sibirica (Carnivora: Mustelidae) in China

2017 ◽  
Vol 3 ◽  
Author(s):  
TIANYU GUO ◽  
YI SUN ◽  
GUANG XU ◽  
LANCE A. DURDEN
Keyword(s):  
New Species ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Sequence Divergence ◽  
Phylogenetic Position ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Closely Related Species ◽  
Ixodes Arboricola ◽  
A New Species

SUMMARY The female and nymph of Ixodes (Pholeoixodes) kangdingensis n. sp. are described based on both morphology and analysis of 16S rRNA gene sequences. Specimens of this new tick species were collected from a Siberian weasel (Mustela sibirica) in Kangding County, Sichuan Province, China. The morphological features of the female and nymph are unique to distinguish I. kangdingensis n. sp. from other members of the subgenus Pholeoixodes, including the presence of distinctly shaped cornua, anterior and posterior processes on palpal article I and a large angular projection on each side of the hypostome. Partial sequence of 16S rRNA gene grouped this species with Ixodes arboricola and Ixodes lividus with sequence divergence of new species from I. arboricola 4·16% and from I. lividus 8·49%. Data on the phylogenetic position, hosts, geographic distribution and key to females of closely related species are also provided.

scholarly journals Description of Eurystomatella sinica n. gen., n. sp., with establishment of a new family Eurystomatellidae n. fam. (Protista, Ciliophora, Scuticociliatia) and analyses of its phylogeny inferred from sequences of the small-subunit rRNA gene

2010 ◽  
Vol 60 (2) ◽  
pp. 460-468 ◽  
Author(s):  
Miao Miao ◽  
Yangang Wang ◽  
Weibo Song ◽  
John C. Clamp ◽  
Khaled A. S. Al-Rasheid
Keyword(s):  
New Genus ◽  
Phylogenetic Analyses ◽  
Sister Group ◽  
Small Subunit ◽  
Rrna Gene ◽  
Small Subunit Rrna ◽  
New Family ◽  
Small Subunit Rrna Gene ◽  
The Family ◽  
Great Divergence

Recently, an undescribed marine ciliate was isolated from China. Investigation of its morphology and infraciliature revealed it as an undescribed species representing a new genus, Eurystomatella n. gen., the type of the new family Eurystomatellidae n. fam. The new family is defined by close-set, apically positioned oral membranelles and a dominant buccal field that is surrounded by an almost completely circular paroral membrane. The new genus is defined by having a small oral membranelle 1 (M1), bipartite M2 and well-developed M3, a body surface faintly sculptured with a silverline system in a quadrangular, reticulate pattern and a cytostome located at the anterior third of a large buccal field. The type species of the new genus, Eurystomatella sinica n. sp., is a morphologically unique form that is defined mainly by the combination of a conspicuously flattened body, several caudal cilia, extremely long cilia associated with the buccal apparatus and a contractile vacuole located subcaudally. According to phylogenetic analyses of small-subunit (SSU) rRNA gene sequences, Eurystomatella clusters with the genus Cyclidium, as a sister group to the family Pleuronematidae. The great divergence in both buccal and somatic ciliature between Eurystomatella and all other known scuticociliates supports the establishment of a new family for Eurystomatella.

Relationships among the three major lineages of the Acari (Arthropoda:Arachnida) inferred from small subunit rRNA: paraphyly of the Parasitiformes with respect to the Opilioacariformes and relative rates of nucleotide substitution

2005 ◽  
Vol 19 (5) ◽  
pp. 383 ◽  
Author(s):  
Anna Murrell ◽  
Susan J. Dobson ◽  
David E. Walter ◽  
Nick J. H. Campbell ◽  
Renfu Shao ◽  
...  
Keyword(s):  
Nucleotide Substitution ◽  
Relative Rate ◽  
Sister Group ◽  
Small Subunit ◽  
High Rate ◽  
Rrna Gene ◽  
Small Subunit Rrna ◽  
Nucleotide Substitutions ◽  
Generation Times ◽  
Nucleotide Substitution Rates

We inferred phylogeny among the three major lineages of the Acari (mites) from the small subunit rRNA gene. Our phylogeny indicates that the Opilioacariformes is the sister-group to the Ixodida+Holothyrida, not the Ixodida+Mesostigmata+Holothyrida, as previously thought. Support for this relationship increased when sites with the highest rates of nucleotide substitution, and thus the greatest potential for saturation with nucleotide substitutions, were removed. Indeed, the increase in support (and resolution) was despite a 70% reduction in the number of parsimony-informative sites from 408 to 115. This shows that rather than ‘noisy’ sites having no impact on resolution of deep branches, ‘noisy’ sites have the potential to obscure phylogenetic relationships. The arrangement, Ixodida+Holothyrida+Opilioacariformes, however, may be an artefact of long-branch attraction since relative-rate tests showed that the Mesostigmata have significantly faster rates of nucleotide substitution than other parasitiform mites. Thus, the fast rates of nucleotide substitution of the Mesostigmata might have caused the Mesostigmata to be attracted to the outgroup in our trees. We tested the hypothesis that the high rate of nucleotide substitution in some mites was related to their short generation times. The Acari species that have high nucleotide substitution rates usually have short generation times; these mites also tend to be more active and thus have higher metabolic rates than other mites. Therefore, more than one factor may affect the rate of nucleotide substitution in these mites.

Description of Tottonophyes enigmatica gen. nov., sp. nov. (Hydrozoa, Siphonophora, Calycophorae), with a reappraisal of the function and homology of nectophoral canals

Zootaxa ◽  
2018 ◽  
Vol 4415 (3) ◽  
pp. 452 ◽  
Author(s):  
P. R. PUGH ◽  
C.W. DUNN ◽  
S.H.D. HADDOCK
Keyword(s):  
New Species ◽  
Phylogenetic Analyses ◽  
Sister Group ◽  
The Other ◽  
Phylogenetic Position ◽  
Unique Combination ◽  
New Family ◽  
Molecular Phylogenetic ◽  
Distinct Morphology ◽  
A New Species

A new species of calycophoran siphonophore, Tottonophyes enigmatica gen. nov, sp. nov., is described. It has a unique combination of traits, some shared with prayomorphs (including two rounded nectophores) and some with clausophyid diphyomorphs (the nectophores are dissimilar, with one slightly larger and slightly to the anterior of the other, and both possess a somatocyst). Molecular phylogenetic analyses indicate that the new species is the sister group to all other diphyomorphs. A new family, Tottonophyidae, is established for it. Its phylogenetic position and distinct morphology help clarify diphyomorph evolution. The function and homology of the nectophoral canals and somatocyst is also re-examined and further clarification is given to their nomenclature.

scholarly journals Characterization of a Novel Hypotrich Ciliate From Heavy Metal-Contaminated Industrial Outlet in Onsan, Ulsan, South Korea

2021 ◽  
Vol 12 ◽  
Author(s):  
Santosh Kumar ◽  
Daizy Bharti ◽  
Shahed Uddin Ahmed Shazib ◽  
Mann Kyoon Shin
Keyword(s):  
New Species ◽  
South Korea ◽  
Phylogenetic Analyses ◽  
Small Subunit ◽  
Rrna Gene ◽  
Small Subunit Rrna ◽  
Gene Sequences ◽  
A Cell ◽  
The Right

Very few studies exist on the description of protozoan ciliates from industrially contaminated sites. In this study, we report a description of a novel hypotrich ciliate isolated from water samples collected from an industrially contaminated outlet in Onsan, Ulsan, South Korea. The oxytrichid ciliate, Histriculus tolerans n. sp., was investigated using live observation and protargol impregnation. The morphology, morphogenesis, and molecular phylogeny inferred from small-subunit (SSU) rRNA gene sequences were studied. The new species is mainly characterized by a cell size of about 70 × 40 μm in vivo, two elongate ellipsoidal macronuclear nodules and one or two micronuclei, adoral zone of about 51% of body length with 32 membranelles on average, about 34 cirri in the right and 24 cirri in the left marginal row, 18 frontoventral transverse cirri, six dorsal kineties including two dorsomarginal rows, and dorsal kinety 1 with 26 bristles. Morphogenesis is similar to that of the type species, i.e., Histriculus histrio, except that oral primordium does not contribute to anlage II of the proter. Phylogenetic analyses, based on small-subunit rRNA gene sequences, consistently place the new species within the family Oxytrichidae, clustering with H. histrio.

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