A new species of Malenchus (Nematoda: Tylenchomorpha) with an updated phylogeny of the Tylenchidae

Summary The family Tylenchidae is one of most abundant and diverse nematode groups found in soil habitats. In this study, a new species named Malenchus cylindricus sp. n. is described based on light and scanning electron microscopy and molecular data. The new species is characterised by elongate-cylindrical vulva-anus body shape and a narrow annulation. We updated the phylogeny of Tylenchidae by including molecular data for the rare genera Miculenchus and Tenunemellus and by improving the morphological characterisation of the genus Lelenchus. Additionally, we compared the effect of alignment methods on the tree topologies and branch support values. The results suggest that the position of subfamily Ecphyadophorinae within Tylenchidae needs to be further revised. Phylogenies inferred from different sequence-based alignments were relatively more similar (i.e., tree topology and branch support) to one another than to secondary structure-aided methods. Finally, conflicting branch support values can be reconciled by properly selecting the alignment method.

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Balantidium grimi n. sp. (Ciliophora, Litostomatea), a new species inhabiting the rectum of the frog Quasipaa spinosa from Lishui, China

Parasite ◽

10.1051/parasite/2018031 ◽

2018 ◽

Vol 25 ◽

pp. 29 ◽

Cited By ~ 1

Author(s):

Weishan Zhao ◽

Can Li ◽

Dong Zhang ◽

Runqiu Wang ◽

Yingzhen Zheng ◽

...

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Phylogenetic Analysis ◽

New Species ◽

Body Shape ◽

Ssu Rdna ◽

The Body ◽

Molecular Phylogenetic ◽

A New Species ◽

Scanning Electron

Balantidium grimi n. sp. is described from the rectum of the frog Quasipaa spinosa (Amphibia, Dicroglossidae) from Lishui, Zhejiang Province, China. The new species is described by both light microscopy (LM) and scanning electron microscopy (SEM), and a molecular phylogenetic analysis is also presented. This species has unique morphological features in that the body shape is somewhat flattened and the vestibulum is “V”-shaped, occupying nearly 3/8 to 4/7 of the body length. Only one contractile vacuole, situated at the posterior body, was observed. The phylogenetic analysis based on SSU-rDNA indicates that B. grimi groups together with B. duodeni and B. entozoon. In addition, the genus Balantidium is clearly polyphyletic.

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Gomphonella olivacea (Bacillariophyceae) – a new phylogenetic position for a well-known taxon, its typification, new species and combinations

Plant Ecology and Evolution ◽

10.5091/plecevo.2019.1603 ◽

2019 ◽

Vol 152 (2) ◽

pp. 219-247 ◽

Cited By ~ 2

Author(s):

Regine Jahn ◽

Wolf-Henning Kusber ◽

Oliver Skibbe ◽

Jonas Zimmermann ◽

Anh Tu Van ◽

...

Keyword(s):

Electron Microscopy ◽

New Species ◽

Molecular Data ◽

Water Bodies ◽

Phylogenetic Position ◽

Lake Balaton ◽

The Family ◽

Clonal Cultures ◽

German Waters ◽

Scanning Electron

Background and aims – Within the project “German Barcode of Life – Diatoms” common diatoms of German waters were routinely isolated and cultivated. In order to understand the taxonomy and phylogeny of the genus Gomphonema, one of the most common taxa of Central Europe, known currently either under the name Gomphonema olivaceum in Europe or Gomphoneis olivacea in America, was studied. Methods – Twenty unialgal strains were established from five different water bodies in Germany and one from Lake Balaton, Hungary, which supplied molecular data (18S V4 and rbcL) besides morphometric and ultrastructural data. In addition, on eight populations from different water bodies including the type from Denmark, morphometric and micromorphological studies by light and scanning electron microscopy were performed. Key results – Molecular and micromorphological data show that the target taxon neither belongs to Gomphonema Ehrenb. nor to Gomphoneis Cleve. By reinstating the genus name Gomphonella Rabenh., the nomenclatural and taxonomic enigma of this taxon is solved, and with the presentation of the type by Hornemann the authorship of the epithet is clarified. Molecular data for the unialgal strains and several environmental clones show that there is more diversity in the Gomphonella olivacea clade than can be identified morphologically. In addition, the establishment of the new species Gomphonella coxiae and Gomphonella acsiae is supported. The molecular data classified Gomphonella species as belonging to the Cymbellales but not to the Gomphonemataceae. In addition, molecular data put Gomphoneis tegelensis R.Jahn & N.Abarca also into Gomphonella. In order to make the genera Gomphoneis and Gomphonema monophyletic, their astigmate members are transferred to Gomphonella. Conclusions – The results clarify that the gomphonemoid outline is not restricted to the family Gomphonemataceae but seem to be distributed across the entire order Cymbellales. This is shown in this paper for the revived genus Gomphonella, which contains the astigmate group of Gomphoneis and Gomphonema besides the longly disputed G. olivacea. Only a polyphasic approach, combining molecular and micromorphological data for taxonomy, nomenclatural evaluation, and observations from clonal cultures can reveal the full intricacies of evolutionary relations.

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Unexpected rDNA divergence between two morphologically minimalistic nematodes with description of a new species (Tylenchomorpha: Tylenchidae)

Nematology ◽

10.1163/15685411-00003195 ◽

2019 ◽

Vol 21 (1) ◽

pp. 57-70 ◽

Cited By ~ 2

Author(s):

Kaikai Qiao ◽

Mengxin Bai ◽

Qiu Wang ◽

Xiangyu Hou ◽

Jingwei Chen ◽

...

Keyword(s):

Electron Microscopy ◽

Genetic Diversity ◽

New Species ◽

Phylogenetic Position ◽

Long Branch Attraction ◽

Large Numbers ◽

The Family ◽

A New Species ◽

Scanning Electron ◽

Phenotypic Convergence

Summary Species of the family Tylenchidae are encountered in large numbers in soils. The genus Labrys was recently described characterised by a remarkable lip pattern that differs from all other known Tylenchidae genera. Here we describe a curious new species, Labrys fujianensis sp. n., that morphologically fits the genus Labrys but which is genetically divergent. The phylogeny was inferred based on 18S and 28S rDNA and light and scanning electron microscopy were used to extract detailed morphologies. The phylogenetic position of this species and its phenotypic convergence are discussed. The possibility of a long-branch attraction artefact was inspected both by removal of variable nucleotide sites and monophyletic testing of topologies. The results confirmed the divergent positioning of the presented species and it is demonstrated that the genetic diversity in Tylenchidae may be much higher than expected due to morphological homoplasy.

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A new species of Polydiscia (Acari, Prostigmata, Tanaupodidae) with reference to its host: a new species of Deuterosminthurus (Collembola, Symphypleona, Bourletiellidae)

Zootaxa ◽

10.11646/zootaxa.188.1.1 ◽

2003 ◽

Vol 188 (1) ◽

pp. 1 ◽

Cited By ~ 3

Author(s):

ENRIQUE BAQUERO ◽

MARIA LOURDES MORAZA ◽

RAFAEL JORDANA

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

New Species ◽

Larval Instar ◽

Undescribed Species ◽

Sampling Campaign ◽

The Family ◽

Parasitic Mites ◽

A New Species ◽

Scanning Electron

During the sampling campaign to describe the Iberian fauna of Collembola, an undescribed species of the family Bourletiellidae living on Genista hispanica L. was found with parasitic mites. The attached parasitic mites were identified as the larval instar of a new species of Polydiscia, a prostigmatid mite of the family Tanaupodidae Thor, 1935. The genus was previously cited in Austria. The Collembola, Deuterosminthurus bisetosus sp. nov, which was found in distant localities on the Iberian Peninsula, and Polydiscia deuterosminthurus sp. nov., are both here described. Both species were found together on Genista for three consecutive years. The abundant material obtained has allowed us to study both species with SEM (Scanning Electron Microscopy), and show characteristics in greater detail than has been possible with light microscopy.

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Rharodesmus Schubart, 1960—a tropical element in the North African fauna: a new species from Tunisia and notes on the family Pyrgodesmidae (Diplopoda: Polydesmida)

Zootaxa ◽

10.11646/zootaxa.2985.1.4 ◽

2011 ◽

Vol 2985 (1) ◽

pp. 55 ◽

Cited By ~ 2

Author(s):

NESRINE AKKARI ◽

HENRIK ENGHOFF

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

New Species ◽

North African ◽

The North ◽

The Family ◽

First Time ◽

A New Species ◽

Scanning Electron

The family Pyrgodesmidae and the genus Rharodesmus Schubart, 1960 are recorded for the first time from Tunisia. A new species, Rharodesmus tabarkensis, is described using scanning electron microscopy, and its membership in the genus and the family is discussed. Notes on west Palaearctic pyrgodesmid species are provided with considerations on the family Pyrgodesmidae.

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A new species of Cribraria (Myxomycetes) from Australia

Novosti sistematiki nizshikh rastenii ◽

10.31111/nsnr/2018.52.2.379 ◽

2018 ◽

Vol 52 (2) ◽

pp. 379-385 ◽

Cited By ~ 1

Author(s):

S. L. Stephenson ◽

Yu. K. Novozhilov ◽

P. Wellman

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

New Species ◽

Lemon Yellow ◽

Moist Chamber ◽

Light Yellow ◽

Dark Violet ◽

The Stability ◽

A New Species ◽

Scanning Electron

A new species of Cribraria, described herein as C. bicolor, appeared in moist chamber cultures on samples of the bark of Eucalyptus sp. collected at two localities in Australia. The morphology of representative specimens was examined by light and scanning electron microscopy, and micrographs of relevant morphological details of sporocarps and spores are provided. The species has a number of distinct and unique morphological features, including a glossy bright-violet globose sporotheca and a two-colored long stalk which is bright-red over the lower one-third and light yellow or lemon-yellow over the upper two-thirds. The combination of these characteristics as well as a shallow calyculus which is dark-violet when viewed under a dissecting microscope and bright red in transmitted light when mounted in lactophenol makes C. bicolor a well-defined morphospecies when compared to all other species of Cribraria. The stability of the taxonomic characters of the species was confirmed by an examination of a number of specimens.

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Description of a new species of Auriculostoma (Digenea: Allocreadiidae) from Characidium heirmostigmata (Characiformes: Crenuchidae) from Argentina, using morphological and molecular data

Journal of Helminthology ◽

10.1017/s0022149x21000109 ◽

2021 ◽

Vol 95 ◽

Author(s):

M.M. Montes ◽

J. Barneche ◽

Y. Croci ◽

D. Balcazar ◽

A. Almirón ◽

...

Keyword(s):

Phylogenetic Analysis ◽

New Species ◽

National Park ◽

Molecular Data ◽

Rrna Gene ◽

28S Rrna ◽

The Family ◽

Parasitological Survey ◽

Morphological And Molecular Data ◽

A New Species

Abstract During a parasitological survey of fishes at Iguazu National Park, Argentina, specimens belonging to the allocreadiid genus Auriculostoma were collected from the intestine of Characidium heirmostigmata. The erection of the new species is based on a unique combination of morphological traits as well as on phylogenetic analysis. Auriculostoma guacurarii n. sp. resembles four congeneric species – Auriculostoma diagonale, Auriculostoma platense, Auriculostoma tica and Auriculostoma totonacapanensis – in having smooth and oblique testes, but can be distinguished by a combination of several morphological features, hosts association and geographic distribution. Morphologically, the new species can be distinguished from both A. diagonale and A. platense by the egg size (bigger in the first and smaller in the last); from A. tica by a shorter body length, the genital pore position and the extension of the caeca; and from A. totonacapanensis by the size of the oral and ventral sucker and the post-testicular space. Additionally, one specimen of Auriculostoma cf. stenopteri from the characid Charax stenopterus (Characiformes) from La Plata River, Argentina, was sampled and the partial 28S rRNA gene was sequenced. The phylogenetic analysis revealed that A. guacurarii n. sp. clustered with A. tica and these two as sister taxa to A. cf. stenopteri. The new species described herein is the tenth species in the genus and the first one parasitizing a member of the family Crenuchidae.

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Encyonema oblonga (Bacillariophyta, Cymbellaceae), a new species from Shanxi Province, China

Phytotaxa ◽

10.11646/phytotaxa.480.3.8 ◽

2021 ◽

Vol 480 (3) ◽

pp. 284-290

Author(s):

QI LIU ◽

JIAJIA LI ◽

QUANXI WANG ◽

JOHN PATRICK KOCIOLEK Patrick Kociolek ◽

Shulian Xie

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

New Species ◽

Shanxi Province ◽

Diatom Flora ◽

A New Species ◽

Scanning Electron ◽

Freshwater Diatom

A new species, Encyonema oblonga Liu & Xie, is collected during a survey of the freshwater diatom flora of Manghe River, Shanxi, China. The valves of this new species are oblong with rounded apices and have the features typical of Encyonema, including complex areolae. We compare the new species with the most similar taxa, E. leei, E. leei var. sinensis and E. appalachianum and consider that E. leei var. sinensis is the valid name. The morphology of E. oblonga is documented with light and scanning electron microscopy.

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Angusticopula rowlingiana, a new melosiroid diatom (Bacillariophyta) from Ascension Island (South Atlantic Ocean)

Phytotaxa ◽

10.11646/phytotaxa.388.2.1 ◽

2019 ◽

Vol 388 (2) ◽

pp. 155 ◽

Cited By ~ 1

Author(s):

BART VAN DE VIJVER ◽

SANDRA WILFERT ◽

VACLAV HOUK ◽

DAVID M. JOHN

Keyword(s):

Electron Microscopy ◽

New Species ◽

Atlantic Ocean ◽

Diatom Species ◽

Ascension Island ◽

Remote Island ◽

A New Species ◽

Similar Combination ◽

Southern Atlantic Ocean ◽

Scanning Electron

During a diatom survey of some samples from Ascension Island, a remote island located in the southern Atlantic Ocean, an unknown melosiroid diatom species was studied using both light and scanning electron microscopy. It proved to be a new species described as Angusticopula rowlingiana sp. nov. and characterized by a large number of narrow copulae in the girdle, a marginal ring of small granules, very small pores covering the entire valve face and occasionally having internal valves. The new species is compared with all Angusticopula species known worldwide and with several Melosira species showing a similar combination of characters. Short notes on its ecology are included.

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Lecanicillium subprimulinum (Cordycipitaceae, Hypocreales), a novel species from Baoshan, Yunnan

Phytotaxa ◽

10.11646/phytotaxa.348.2.4 ◽

2018 ◽

Vol 348 (2) ◽

pp. 99 ◽

Cited By ~ 7

Author(s):

SHIKE HUANG ◽

SAJEEWA S.N. MAHARACHCHIKUMBURA ◽

RAJESH JEEWON ◽

D JAYARAMA BHAT ◽

RUNGTIWA PHOOKAMSAK ◽

...

Keyword(s):

New Species ◽

Novel Species ◽

Phylogenetic Analyses ◽

Molecular Data ◽

Its Sequence ◽

New Taxon ◽

Sexual Morph ◽

The Family ◽

Asexual Morphs ◽

A New Species

In this paper, we report a new asexual-morph taxon belonging to the family Cordycipitaceae (Hypocreales), which is associated with a sexual morph of an ophioceras-like taxon on submerged wood collected from Baoshan, Yunnan, China. Morphologically, this new taxon is similar to known asexual morphs of Lecanicillium and characterized by conidiophores arising from hyaline hyphae, with gregarious, ellipsoid to ovoid, aseptate conidia. Phylogenetic analyses of a combined LSU, SSU, tef1-α and ITS sequence dataset positions our taxon in Cordycipitaceae and close to Lecanicillium sp. (CBS 639.85) and L. primulinum. Lecanicillium subprimulinum is introduced as a new species with support from molecular data.

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