Paratylenchus jasmineae sp. n. (Nematoda: Paratylenchinae) from rhizosphere of Jasminum sambac in India

Nematology ◽  
2019 ◽  
Vol 21 (5) ◽  
pp. 469-478 ◽  
Author(s):  
Victor Phani ◽  
Vishal Singh Somvanshi ◽  
Uma Rao ◽  
Matiyar Rahaman Khan

Summary A new species of pin nematode, Paratylenchus jasmineae sp. n., was isolated and described from the rhizosphere of jasmine in India. The new species can be diagnosed by having a relatively larger body length of 248-355 μm, stylet length of 24.6-28.5 μm, lateral field with four lateral lines, the presence of small post-vulval uterine sac and absence of advulval flaps in the females. Additional morphological characters included continuous lip region with small projected submedian lobes, large and oblong-shaped spermatheca, the presence of prevulval swelling, and arcuate conoid tail with finely rounded terminus. Molecular characterisation of the species was carried out based on internal transcribed spacer sequence (ITS rRNA) and D2-D3 expansion segment of 28S rRNA subunit. Phylogenetic analyses based on ITS and D2-D3 molecular markers resulted in clear separation of P. jasmineae sp. n. from other Paratylenchus species.

Nematology ◽  
2020 ◽  
Vol 22 (8) ◽  
pp. 843-853
Author(s):  
Jianfeng Gu ◽  
Munawar Maria ◽  
Yiwu Fang ◽  
Xianfeng Chen ◽  
Lele Liu

Summary Laimaphelenchus spiflatus n. sp. isolated from declining Chinese pine, Pinus tabuliformis, is described and illustrated. The new species can be characterised by its relatively long body size of 1150 ± 108 (976-1437) μm for females and 1092 ± 78.6 (905-1235) μm for males, lateral field with four lines, females with a long vulval flap, and tail conoid, slightly ventrally curved and ending in a stalk having 8-12 projections when observed with SEM. Males are common, with 27.3 (23.4-28.8) μm long spicules having blunt (not well-developed) condylus and rostrum and truncate simple distal tip, and four caudal papillae. By having a vulval flap, four lateral lines and tail end with a stalk (without four tubercles), the new species comes close to L. preissii, L. simlaensis, and L. unituberculus, but can be differentiated from them by morphological characters and morphometric data. In phylogenetic analyses using near full length 18S and D2-D3 expansion segments of 28S rDNA, Laimaphelenchus spiflatus n. sp. appeared as an independent lineage separated from the other Laimaphelenchus spp. that are currently sequenced for their aforementioned genomic regions.


Nematology ◽  
2020 ◽  
pp. 1-10
Author(s):  
Manouchehr Hosseinvand ◽  
Ali Eskandari ◽  
Reza Ghaderi

Summary A new species of Coslenchus from north-western Iran is illustrated and described using morphological, morphometric and molecular data. Coslenchus persicus n. sp. is characterised by a medium-sized body of 694-810 μm, often 18 (seldom 20-22) longitudinal ridges excluding lateral field, head with four distinct annuli, stylet 9.6-10.8 μm long, pharyngeal basal bulb large and cylindrical, spermatheca offset and ovate, without sperm, vulval flaps extending for two annuli, 5-7 μm in length, tail striated with finely to bluntly rounded tip, 110-153 μm long and without males. The new species comes close to C. assamensis, C. diversus, C. japonicus, C. leiocephalus, C. maritus, C. multigyrus, C. paramaritus, C. pastor, and C. polonicus, but can be distinguished from all by differences in body length, stylet length and shape of basal bulb, post-vulval uterine sac, tail and certain morphometric data. Molecular data are provided for the characterisation of the new species using ribosomal genes (18S and 28S rRNA). In the 18S rRNA tree, the new species was placed in a clade including one isolate of Coslenchus sp. from Iran. In the reconstructed 28S rRNA phylogenetic tree, C. persicus n. sp. clustered with other known Coslenchus species.


Nematology ◽  
2016 ◽  
Vol 18 (1) ◽  
pp. 29-37 ◽  
Author(s):  
Mehrab Esmaeli ◽  
Ramin Heydari ◽  
Pablo Castillo ◽  
Juan E. Palomares-Rius

A new species of the genus Nothotylenchus, N. persicus n. sp. was collected around the roots of grapevine and is described and illustrated herein based on morphological and molecular studies. The new species is characterised by a body length of 776-900 μm, delicate stylet 5-6 μm long, six lines in the lateral field, post-vulval uterine sac short, 10-18 μm long, female tail elongate-conoid with pointed terminus, and bursa covering 40-45% of tail length. Morphologically, N. persicus n. sp. appears closer to four known species of the genus, namely: N. hexaglyphus, N. affinis, N. medians and N. taylori. The results of phylogenetic analyses based on sequences of D2-D3 expansion region of 28S rRNA gene confirmed the close molecular relationship between N. persicus n. sp. and other anguinids, but Nothotylenchus claded separately from Ditylenchus species.


Nematology ◽  
2018 ◽  
Vol 20 (7) ◽  
pp. 653-669 ◽  
Author(s):  
Oleg Gorgadze ◽  
Elena Fanelli ◽  
Manana Lortkhipanidze ◽  
Alberto Troccoli ◽  
Medea Burjanadze ◽  
...  

Summary A new species of entomopathogenic nematode, Steinernema borjomiense n. sp., was isolated from the body of the host insect, Oryctes nasicornis (Coleoptera: Scarabaeidae), in Georgia, in the territory of Borjomi-Kharagauli. Morphological characters indicate that the new species is closely related to species of the feltiae-group. The infective juveniles are characterised by the following morphological characters: body length of 879 (777-989) μm, distance between the head and excretory pore = 72 (62-80) μm, pharynx length = 132 (122-142) μm, tail length = 70 (60-80) μm, ratio a = 26.3 (23.0-29.3), H% = 45 (40-51), D% = 54 (47-59), E% = 102 (95-115), and lateral fields consisting of seven ridges (eight incisures) at mid-body. Steinernema borjomiense n. sp. was molecularly characterised by sequencing three ribosomal regions (the ITS, the D2-D3 expansion domains and the 18S rRNA gene) and the mitochondrial COI gene. Phylogenetic analyses revealed that S. borjomiense n. sp. differs from all other known species of Steinernema and is a member of the monticolum-group.


Phytotaxa ◽  
2017 ◽  
Vol 312 (1) ◽  
pp. 111
Author(s):  
HUAN-DI ZHENG ◽  
WEN-YING ZHUANG

A new species, namely Chlorociboria herbicola, is discovered on herbaceous stems in central China. Morphologically, the new fungus is distinctive by the combination of light blue-green apothecia, rectangular cells in ectal excipulum, and elongate-ellipsoidal ascospores with rounded ends. Phylogenetic analyses of the internal transcribed spacer and large subunit of nuclear ribosomal DNA sequences confirm its ascription in Chlorociboria and distinction from the known species of the genus.


2021 ◽  
Vol 5 (2) ◽  
pp. 177-187
Author(s):  
Lourdes Y. Echevarría ◽  
Pablo J. Venegas ◽  
Luis A. García-Ayachi ◽  
Pedro M. Sales Nunes

We describe a new species of Selvasaura from the montane forests of the eastern slopes of the Andes in northern Peru, based on external and hemipenial morphological characters and previous phylogenetic analyses. The new species can be differentiated from the other two Selvasaura species in having keeled dorsal scales usually flanked by longitudinal striations, in adults and juveniles; adult males with a yellow vertebral stripe bordered by broad dark brown stripes on each side and a unilobed hemipenis surrounded by the branches of the sulcus spermaticus. The description of the new species contributes information about new states of diagnostic characters of Selvasaura and natural history.


MycoKeys ◽  
2020 ◽  
Vol 75 ◽  
pp. 1-29
Author(s):  
Komsit Wisitrassameewong ◽  
Myung Soo Park ◽  
Hyun Lee ◽  
Aniket Ghosh ◽  
Kanad Das ◽  
...  

Russula subsection Amoeninae is morphologically defined by a dry velvety pileus surface, a complete absence of cystidia with heteromorphous contents in all tissues, and spores without amyloid suprahilar spot. Thirty-four species within subsection Amoeninae have been published worldwide. Although most Russula species in South Korea have been assigned European or North American names, recent molecular studies have shown that Russula species from different continents are not conspecific. Therefore, the present study aims to: 1) define which species of Russula subsection Amoeninae occur on each continent using molecular phylogenetic analyses; 2) revise the taxonomy of Korean Amoeninae. The phylogenetic analyses using the internal transcribed spacer (ITS) and multilocus sequences showed that subsection Amoeninae is monophyletic within subgenus Heterophyllidiae section Heterophyllae. A total of 21 Russula subsection Amoeninae species were confirmed from Asia, Australia, Europe, North America, and Central America, and species from different continents formed separate clades. Three species were recognized from South Korea and were clearly separated from the European and North American species. These species are R. bella, also reported from Japan, a new species described herein, Russula orientipurpurea, and a new species undescribed due to insufficient material.


Zootaxa ◽  
2011 ◽  
Vol 2737 (1) ◽  
pp. 19 ◽  
Author(s):  
LUIZ NORBERTO WEBER ◽  
VANESSA KRUTH VERDADE ◽  
RODRIGO DE DE OLIVEIRA LULA SALLES ◽  
ANTOINE FOUQUET ◽  
SERGIO POTSCH DE CARVALHO-E-SILVA

We report here the discovery of a new species of frog associated to the open areas of the highlands of the Parque Nacional da Serra dos Órgãos. The new species, Cycloramphus organensis is characterized by a unique skin texture, medium size (maximum male and female SVL 26.4 mm and 33.3 mm respectively), dorsal surfaces uniformly brick red colored, uniformly areolate skin on dorsum, pupil horizontal, iris with a menisc on upper margin; no fleshy tubercles on eyelid, tympanic annulus concealed beneath skin, macroglands not visible externally, fingers and toes without fringes and webs; supernumerary palmar and plantar tubercles absent, nuptial spines absent. Despite the presence of an iris menisc, a character shared by frogs of both genera Cycloramphus and Zachaenus Cope, the combination of morphological characters is so unique that the allocation of the species to any of these genera remains ambiguous. Consequently, we used additional molecular-based phylogenetic analyses to ascertain the position of the new taxon. The new species proved to be embedded within the genus Cycloramphus.


Phytotaxa ◽  
2019 ◽  
Vol 427 (1) ◽  
pp. 31-42
Author(s):  
LEI SHU ◽  
RUI-LIANG ZHU

Based on molecular phylogenetic analyses and morphological characters, a new species from Bangladesh, northern Vietnam, and southwestern China, Leptolejeunea nigra, is described. It is mostly similar to L. balansae but remarkable for having brownish black ocelli in its leaf lobes. In the molecular phylogeny, the samples of L. nigra are not nested within any clade and form an independent lineage. In particular, the molecular dating suggested that the divergence of L. nigra happened in time span of the formation of the Himalayas.


Phytotaxa ◽  
2018 ◽  
Vol 334 (2) ◽  
pp. 183 ◽  
Author(s):  
Fatima El-Houaria ZITOUNI-HAOUAR ◽  
JUAN RAMÓN CARLAVILLA ◽  
GABRIEL MORENO ◽  
JOSÉ LUIS MANJÓN ◽  
ZOHRA FORTAS

Morphological and phylogenetic analyses of large ribosomal subunit (28S rDNA) and internal transcribed spacer (ITS rDNA) of Terfezia samples collected from several bioclimatic zones in Algeria and Spain revealed the presence of six distinct Terfezia species: T. arenaria, T. boudieri, T. claveryi; T. eliocrocae (reported here for the first time from North Africa), T. olbiensis, and a new species, T. crassiverrucosa sp. nov., proposed and described here, characterized by its phylogenetic position and unique combination of morphological characters. A discussion on the unresolved problems in the taxonomy of the spiny-spored Terfezia species is conducted after the present results.


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