Additional data on Pratylenchoides riparius (Andrássy, 1985) Luc, 1986 (Nematoda: Merliniidae) from Iran

Nematology ◽  
2019 ◽  
Vol 21 (8) ◽  
pp. 827-836 ◽  
Author(s):  
Manouchehr Hosseinvand ◽  
Ali Eskandari ◽  
Reza Ghaderi
Keyword(s):  
Taxonomic Status ◽  
Molecular Data ◽  
18S Rrna Gene ◽  
Rrna Gene ◽  
Similar Species ◽  
28S Rrna ◽  
Lateral Field ◽  
Molecular Phylogenetic ◽  
Type Population ◽  
Stylet Length

Summary The second population of Pratylenchoides riparius, including females and males, is described and illustrated based upon morphological, morphometric and molecular data. The present population from Iran is characterised by some differences with the type population of the species from Hungary in stylet length (24-26 vs 21-22 μm), slightly longer body (1002-1230 vs 830-960 μm), pharynx (202-211 vs 182-190 μm) and tail (64-85 vs 48-57 μm), areolated outer bands of the lateral field (vs non-areolated), widening of the lateral field near tail terminus (vs lateral incisures connecting each other) and presence of males (vs absent). The taxonomic status of the species with regarding the data from the type and presently recovered population, as well as the closely similar species is discussed. The newly recovered population was studied based upon its molecular phylogenetic charactes using the D2-D3 of 28S rRNA and the partial 18S rRNA gene sequences and the results revealed that it forms a clade with P. magnicauda in 28S, but occupies a distant placement from it in 18S phylogeny.

Morphological redescription and molecular characterization of Trichodina matsu Basson & Van As, 1994 (Ciliophora, Mobilida, Trichodinidae) infecting Tachysurus fulvidraco (Richardson, 1846) from Chongqing, China

Zootaxa ◽  
2021 ◽  
Vol 4995 (2) ◽  
pp. 334-344
Author(s):  
QIAN ZHOU ◽  
FAHUI TANG ◽  
YUANJUN ZHAO
Keyword(s):  
Phylogenetic Analyses ◽  
Molecular Data ◽  
18S Rrna Gene ◽  
Rrna Gene ◽  
Similar Species ◽  
Sequence Alignments ◽  
Multiple Sequence ◽  
Multiple Sequence Alignments ◽  
Morphological And Molecular Data ◽  
Tachysurus Fulvidraco

During a survey of parasitic ciliates in Chongqing, China, Trichodina matsu Basson & Van As, 1994 was isolated from gills of Tachysurus fulvidraco. Furthermore, the 18S rRNA gene and ITS-5.8S rRNA region of T. matsu were sequenced for the first time and applied for the species identification and comparison with similar species in the present study. Based on the morphological and molecular comparisons, the results indicate that T. matsu is an ectoparasite specific for the Siluriformes catfish. Based on the analyses of genetic distance, multiple sequence alignments, and phylogenetic analyses, no obvious differentiation within populations of T. matsu was found. In addition, the ‘Trichodina hyperparasitis’ (KX904933) in GenBank is a misidentification and appears to be conspecific with T. matsu according to the comparison of morphological and molecular data.  

Morphological and molecular characterisation of Coslenchus persicus n. sp. (Nematoda: Tylenchidae) from Iran

Nematology ◽  
2020 ◽  
pp. 1-10
Author(s):  
Manouchehr Hosseinvand ◽  
Ali Eskandari ◽  
Reza Ghaderi
Keyword(s):  
New Species ◽  
Molecular Data ◽  
Ribosomal Genes ◽  
28S Rrna ◽  
Lateral Field ◽  
Rrna Tree ◽  
Basal Bulb ◽  
Stylet Length ◽  
North Western ◽  
A New Species

Summary A new species of Coslenchus from north-western Iran is illustrated and described using morphological, morphometric and molecular data. Coslenchus persicus n. sp. is characterised by a medium-sized body of 694-810 μm, often 18 (seldom 20-22) longitudinal ridges excluding lateral field, head with four distinct annuli, stylet 9.6-10.8 μm long, pharyngeal basal bulb large and cylindrical, spermatheca offset and ovate, without sperm, vulval flaps extending for two annuli, 5-7 μm in length, tail striated with finely to bluntly rounded tip, 110-153 μm long and without males. The new species comes close to C. assamensis, C. diversus, C. japonicus, C. leiocephalus, C. maritus, C. multigyrus, C. paramaritus, C. pastor, and C. polonicus, but can be distinguished from all by differences in body length, stylet length and shape of basal bulb, post-vulval uterine sac, tail and certain morphometric data. Molecular data are provided for the characterisation of the new species using ribosomal genes (18S and 28S rRNA). In the 18S rRNA tree, the new species was placed in a clade including one isolate of Coslenchus sp. from Iran. In the reconstructed 28S rRNA phylogenetic tree, C. persicus n. sp. clustered with other known Coslenchus species.

scholarly journals Molecular and morphological characterisation of Longidorus polyae sp. n. and L. pisi Edward, Misra & Singh, 1964 (Dorylaimida, Longidoridae) from Bulgaria

ZooKeys ◽  
2019 ◽  
Vol 830 ◽  
pp. 75-98 ◽  
Author(s):  
Stela S. Lazarova ◽  
Milka Elshishka ◽  
Georgi Radoslavov ◽  
Lydmila Lozanova ◽  
Peter Hristov ◽  
...  
Keyword(s):  
New Species ◽  
Body Length ◽  
Nematode Species ◽  
Molecular Data ◽  
18S Rrna Gene ◽  
The Body ◽  
Integrative Approach ◽  
Rrna Gene ◽  
28S Rrna ◽  
Stage Juvenile

Longidoruspolyaesp. n., a bisexual nematode species found in the rhizosphere of pear tree (Pyruscommunis L.), is described and characterised using an integrative approach. The new species has a female body length of 6.8–9.1 mm; a comparatively long odontostyle (114.0–127.5 μm); a narrow lip region (14.0–15.5 μm), anteriorly flattened and almost continuous with the body profile; pocket-like amphidial pouches long, deeply bilobed, and slightly asymmetrical, a guide ring at 37–42 μm from the anterior end; normal arrangement of pharyngeal glands; and a short bluntly rounded to hemispherical tail. Four juvenile stages identified: the first stage with a digitate tail, and the second and subsequent stages with a bluntly rounded tail. Males have one adcloacal pair and a row of 10 or 11 single ventromedian supplements; spicules 71.0–74.5 μm long. Based on morphometric data, the new species belongs to a group of species spread over Europe (L.arthensis, L.silvae, L.uroshis,), Iran (L.kheirii), and Syria (L.pauli), which share common characters such as amphidial fovea, lip region and tail shapes, similar odontostyle and body length, and similar first-stage juvenile tail shape. Codes for identifying the new species are A5, B2, C34, D3, E3, F45, G12, H1, I2, J1, K7. The phylogenetic analysis based on D2-D3 expansion domains of the rRNA gene revealed that the new species has the closest relationships with L.athesinus from Italy and three unidentified Longidorus spp. from USA (Longidorus sp. 1, Longidorus sp. 2, and Longidorus sp. 6). New morphometric and molecular data (18S rRNA gene, ITS1-5.8S-ITS2 regions and D2-D3 28S rRNA gene sequences) for three populations of L.pisi from Bulgaria were obtained and variations between populations are discussed.

scholarly journals A revision of the Rhoptrobothriidae (Cestoda: “Tetraphyllidea”)

Zootaxa ◽  
2021 ◽  
Vol 4999 (3) ◽  
pp. 201-218
Author(s):  
KIRSTEN JENSEN ◽  
ISABEL A. M. PEN ◽  
JANINE N. CAIRA
Keyword(s):  
Sri Lanka ◽  
Sequence Data ◽  
Taxonomic Status ◽  
Molecular Data ◽  
Rrna Gene ◽  
28S Rrna ◽  
Family Status ◽  
The Family ◽  
New Material ◽  
Single Genus

The Rhoptrobothriidae are one of the more enigmatic families of cestodes of elasmobranchs. Opinions on the taxonomic status of the family’s three original genera (i.e., Myzophyllobothrium, Rhoptrobothrium, and Myzocephalus) have varied over the 115 years since they were erected. Some authors have considered all three valid, others have considered Rhoptrobothrium to be a synonym of Myzopyllobothrium or a genus inquirendum, yet others have considered Myzocephalus to be a synonym of the phyllobothriid genus Thysanocephalum. All three genera were established for specimens collected from eagle rays off Sri Lanka. The erection of Mixophyllobothrium for two specimens from a cowtail stingray off India three decades ago added additional confusion to the situation, with some authors considering it valid and others a synonym of Myzocephalus. These disagreements stem largely from differences in interpretation of the complex morphology of the scolex of members of these genera. Furthermore, with the exception of Rhoptrobothrium comprising four species, each genus is monotypic. All but Rhoptrobothrium has not been considered in detail for nearly a century, largely because of a lack of available material. The taxonomic status of these genera is assessed here based on light and scanning electron microscopy, and molecular data generated from new material collected from eagle rays off Indonesian and Malaysian Borneo, Japan, Sri Lanka, and Viet Nam. Morphological work indicates that the genera differ largely only in the degree of folding of the four remi that extend from the cephalic peduncle. A molecular phylogeny based on sequence data for the D1–D3 region of the 28S rRNA gene, which include new data for eight specimens of four species, indicates that Myzophyllobothrium, Myzocephalus, and Rhoptrobothrium are not mutually monophyletic. The latter two genera and Mixophyllobothrium are considered synonyms of Myzophyllobothrium and five species are transferred to that genus. Myzophyllobothrium okamuri n. comb. is considered a species inquirendum. Myzophyllobothrium nagasawai n. sp. is described from Aetobatus narutobiei off Japan. Myzophyllobothrium narinari n. comb. is re-described based on newly collected cestodes from the type host and locality (i.e., Aetobatus ocellatus off Sri Lanka). Despite consisting of only a single genus, the family status of the group is retained in recognition of the unusual configuration of the scolex, which bears four biloculate bothridia and four remi extending from the cephalic peduncle. The ordinal placement of the family remains uncertain, but affinities with the Phyllobothriidea, rather than “Tetraphyllidea” are considered.

Molecular phylogeny and systematics of cestodes with rudimentary rostellum (Cestoda: Hymenolepididae) from Holarctic Sorex shews (Eulipotyphla: Soricidae)

2019 ◽  
Vol 187 (3) ◽  
pp. 965-986
Author(s):  
Svetlana A Kornienko ◽  
Rasa Binkienė ◽  
Nikolai E Dokuchaev ◽  
Vasyl V Tkach
Keyword(s):  
Phylogenetic Analysis ◽  
Molecular Phylogeny ◽  
Type Species ◽  
Taxonomic Status ◽  
Genetic Data ◽  
Molecular Study ◽  
Morphological Characters ◽  
Rrna Gene ◽  
Similar Species ◽  
28S Rrna

Abstract We analyse the phylogenetic relationships and taxonomic status of hymenolepidid cestodes with rudimentary rostellum, parasitic in Holarctic shrews of the genus Sorex. These cestodes were previously termed as a ‘Ditestolepis clade’ and encompass eight genera: Diorchilepis, Ditestolepis, Ecrinolepis, Gulyaevilepis, Mathevolepis, Novobrachylepis, Spalania and Spasskylepis. Representatives of seven of these were available for molecular study, including type species of all seven genera. Phylogenetic analysis based on partial sequences of the 28S rRNA gene supports this group of genera as monophyletic. The ‘Ditestolepis clade’ consists of two subclades. One of these includes genera containing cestodes having suckers positioned inside bothrium-like depressions (Ditestolepis, Gulyaevilepis and Spasskylepis). The second subclade contains cestodes with suckers expanding far beyond the margins of the scolex (Mathevolepis, Ecrinolepis, Spalania and Novobrachylepis). The relative taxonomic value of several additional morphological characters is discussed. Genetic data convincingly demonstrate that Ditestolepis diaphana, Spasskylepis ovaluteri and Mathevolepis macyi represent complexes of morphologically similar species. Based on the phylogenetic analysis, we resurrect the genus Spalania. Amended generic diagnoses and keys to identification of the genera in the Ditestolepis clade, as well as keys to the known species, are provided.

Morphological and molecular characterisation of Xiphinema ifacolum Luc, 1961 (Nematoda: Longidoridae) from Sri Lanka

Nematology ◽  
2018 ◽  
Vol 20 (10) ◽  
pp. 925-937 ◽  
Author(s):  
Solomia Susulovska ◽  
Carolina Cantalapiedra-Navarrete ◽  
Andrij Susulovsky ◽  
Pablo Castillo ◽  
Antonio Archidona-Yuste
Keyword(s):  
Sri Lanka ◽  
18S Rrna ◽  
Mitochondrial Gene ◽  
Molecular Data ◽  
18S Rrna Gene ◽  
Rrna Gene ◽  
28S Rrna ◽  
Molecular Characterisation ◽  
Group 4 ◽  
Sao Tome And Principe

Summary Females and juveniles from a population of Xiphinema ifacolum from Sri Lanka are described based on morphology, morphometrics and molecular analyses. Morphologically, females and juveniles from Sri Lanka are similar to original descriptions and other reports from Brazil, Cameroon, Liberia, and São Tomé and Príncipe. The identity of the species was also confirmed by 18S rRNA gene sequences deposited in NCBI from Brazil (AY297826). Integrative diagnosis was completed with molecular data using D2-D3 expansion segments of 28S rRNA, ITS1 region, partial 18S-rRNA and the partial mitochondrial gene cytochrome c oxidase subunit 1 (coxI). This is the third molecular characterisation for a species of the X. non-americanum Group 4, after X. oleae and X. tica. The use of different ribosomal and mitochondrial markers in this study, particularly, D2-D3, ITS1 and partial coxI, provided a precise and unequivocal tool for the identification of X. ifacolum and contributes to a better knowledge of the diversity within Xiphinema. Morphospecies Group 4 appears to be a paraphyletic group within the X. non-americanum assemblage.

Hemicriconemoides phoenicis Van den Berg et al., 2015 (Nematoda: Criconematidae) from Iran: a morphological and molecular phylogenetic study

Nematology ◽  
2020 ◽  
Vol 22 (7) ◽  
pp. 815-824
Author(s):  
Sedighe Azimi ◽  
Majid Pedram
Keyword(s):  
Date Palm ◽  
Phylogenetic Analyses ◽  
Original Description ◽  
Original Data ◽  
18S Rrna Gene ◽  
Phylogenetic Study ◽  
Rrna Gene ◽  
28S Rrna ◽  
Khuzestan Province ◽  
Molecular Phylogenetic

Summary A population of Hemicriconemoides phoenicis was recovered from Khuzestan province, south-western Iran, in association with date palm. The recovered population was characterised by 518-645 μm long females having a 76-82 μm long stylet, rounded to oblong spermatheca filled with sperm, a 28.0-39.8 μm long tail, juveniles common, with 14 longitudinal rows of rounded scales, and males absent. Compared to the original data, no morphological and morphometric differences were observed. In molecular phylogenetic analyses using the D2-D3 expansion segments of the 28S rRNA gene and a near-full-length fragment of the 18S rRNA gene sequences using Bayesian inference (BI) and maximal number of species of the genus, the two newly generated 28S sequences of the Iranian population formed a maximally supported clade with two original sequences of the species; and the 18S sequence formed a maximally supported clade with an unidentified isolate of the genus in the corresponding phylogeny. This is the second report of the species since its original description, Iran representing a new geographical record and supporting the suggestion that date palm could be its preferred host.

Bursaphelenchus juglandis n. sp. (Nematoda: Aphelenchoididae), an associate of walnut twig beetle, Pityophthorus juglandis, the vector of thousand cankers disease

Nematology ◽  
2020 ◽  
pp. 1-30
Author(s):  
Alexander Y. Ryss ◽  
Corwin Parker ◽  
Sergio Álvarez-Ortega ◽  
Steven A. Nadler ◽  
Sergei A. Subbotin
Keyword(s):  
Developmental Stages ◽  
Rrna Genes ◽  
Rrna Gene ◽  
Similar Species ◽  
28S Rrna ◽  
Lateral Field ◽  
Pityophthorus Juglandis ◽  
Thousand Cankers Disease ◽  
Species Specific ◽  
Walnut Twig Beetle

Summary Bursaphelenchus juglandis n. sp. was isolated from the walnut twig beetle, Pityophthorus juglandis, and walnut trees, Juglans spp. with symptoms of thousand cankers disease, in California, USA. Based on analysis of three rRNA genes and morphological features (three lines in lateral field, small arched vulval flap in female, broad spicule with two lines along blade and small cucullus, digitate dorsally bent condylus, male tail pattern of five papilliform papillae and one pair of glandpapillae (P5), and curved conical female tail), the new species belongs to the Abietinus group within Bursaphelenchus. It differs from similar species of this group by the presence of a cephalic disc with lateral labial sensilla at the disc border, and in having thick spicules with the capitulum surface almost parallel to a virtual direct line extending from the spicule end. An emended diagnosis, tabular polytomous identification key and compendium of species with the lists of their vectors, plant hosts, and distribution are provided for the Abietinus group. The diagnostics of the propagative developmental stages is given, including sex differences; the transmission dauer stage was identified as the third stage and its description given with sexual differences. A molecular phylogeny of Bursaphelenchus is provided based on partial 18S rRNA, ITS rRNA and the D2-D3 expansion fragments of 28S rRNA gene sequences. A PCR with a species-specific primer was developed for detection of B. juglandis n. sp.

scholarly journals NEW MORPHOLOGICAL OBSERVATIONS ON PRATYLENCHUS THORNEI SHER & ALLEN, 1953 (NEMATODA PRATYLENCHIDAE) BASED ON THE POPULATION FROM IRAN

Redia ◽  
2020 ◽  
Vol 103 ◽  
pp. 89-100
Author(s):  
EBRAHIM MOVAHEDIFAR ◽  
SEDIGHE AZIMI
Keyword(s):  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Molecular Data ◽  
Similar Species ◽  
Lateral Field ◽  
Khuzestan Province ◽  
Pratylenchus Thornei ◽  
Molecular Phylogenetic ◽  
Morphological And Molecular Data ◽  
Southwest Iran

A population of Pratylenchus thornei was recovered from the rhizospheric soil of sugarcane in Khuzestan province, southwest Iran. It was studied using morphological and molecular data and new morphological observations were made. This population is characterized by 457-551 μm long females, lateral field with five or six smooth incisures, lip region with three annuli, stylet 14.0-16.8 μm long with rounded basal knobs, spermatheca empty, tail subcylindrical and slightly conical towards tip, tail terminus truncate, subdigitate, trilobed or indented. Comparisons with some previously reported populations and similar species are discussed. This is the first report of the species from sugarcane fields in Iran. The molecular phylogenetic analyses were done by using partial sequences of the D2-D3 expansion segments of large subunit, and internal transcribed spacer (LSU D2-D3 and ITS rDNA) regions. The studied population of the species formed a maximally supported clade with other sequences of the species in both phylogenetic analyses using Bayesian inference

Description of a new species of Auriculostoma (Digenea: Allocreadiidae) from Characidium heirmostigmata (Characiformes: Crenuchidae) from Argentina, using morphological and molecular data

2021 ◽  
Vol 95 ◽  
Author(s):  
M.M. Montes ◽  
J. Barneche ◽  
Y. Croci ◽  
D. Balcazar ◽  
A. Almirón ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
National Park ◽  
Molecular Data ◽  
Rrna Gene ◽  
28S Rrna ◽  
The Family ◽  
Parasitological Survey ◽  
Morphological And Molecular Data ◽  
A New Species

Abstract During a parasitological survey of fishes at Iguazu National Park, Argentina, specimens belonging to the allocreadiid genus Auriculostoma were collected from the intestine of Characidium heirmostigmata. The erection of the new species is based on a unique combination of morphological traits as well as on phylogenetic analysis. Auriculostoma guacurarii n. sp. resembles four congeneric species – Auriculostoma diagonale, Auriculostoma platense, Auriculostoma tica and Auriculostoma totonacapanensis – in having smooth and oblique testes, but can be distinguished by a combination of several morphological features, hosts association and geographic distribution. Morphologically, the new species can be distinguished from both A. diagonale and A. platense by the egg size (bigger in the first and smaller in the last); from A. tica by a shorter body length, the genital pore position and the extension of the caeca; and from A. totonacapanensis by the size of the oral and ventral sucker and the post-testicular space. Additionally, one specimen of Auriculostoma cf. stenopteri from the characid Charax stenopterus (Characiformes) from La Plata River, Argentina, was sampled and the partial 28S rRNA gene was sequenced. The phylogenetic analysis revealed that A. guacurarii n. sp. clustered with A. tica and these two as sister taxa to A. cf. stenopteri. The new species described herein is the tenth species in the genus and the first one parasitizing a member of the family Crenuchidae.

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