Bursaphelenchus hibisci n. sp. (Nematoda: Aphelenchoididae) isolated from Ernoporus corpulentus (Scolytinae) emerged from dead Hibiscus tiliaceus (Malvaceae) twigs from Okinawa, Japan

Nematology ◽  
2021 ◽  
pp. 1-16
Author(s):  
Natsumi Kanzaki ◽  
Satoshi Tsujimoto ◽  
Hisashi Kajimura
Keyword(s):  
Bark Beetle ◽  
Subtropical Region ◽  
Male And Female ◽  
Female Tail ◽  
Lateral Lines

Summary A Bursaphelenchus sp. belonging to the B. idius-group was isolated from a bark beetle, Ernoporus corpulentus, which had emerged from dead twigs of Hibiscus tiliaceus collected in Okinawa, in the subtropical region of Japan. In addition to its group-specific characters, i.e., four lateral lines in both male and female and less developed capitulum and relatively broad blade of the male spicules, the species is characterised by a relatively slender female tail with various tip shapes. Molecularly, the species is close to B. fagi and B. tiliae based on SSU and D2-D3 LSU sequences, but can be clearly distinguished from these two species. The species is described and illustrated herein as B. hibisci n. sp.

Redescription of Bursaphelenchus eucarpus Rühm, 1956 (Nematoda: Aphelenchoididae) associated with the apple bark beetle, Scolytus mali Bechstein, and the shothole borer, S. rugulosus Müller

Nematology ◽  
2018 ◽  
Vol 20 (9) ◽  
pp. 889-903 ◽  
Author(s):  
Jianfeng Gu ◽  
Marek Tomalak ◽  
Helen Braasch ◽  
Yiwu Fang
Keyword(s):  
Bark Beetle ◽  
Related Species ◽  
Original Description ◽  
Morphological Features ◽  
Prunus Padus ◽  
Female Tail ◽  
Lateral Lines ◽  
The Common ◽  
Tail Tip ◽  
Partial 18S

Summary New morphological, molecular and bionomic data are added to update the description of Bursaphelenchus eucarpus. The nematode was isolated from larval galleries and adults of the apple bark beetle, Scolytus mali, and the shothole borer, S. rugulosus, present under the bark of the bird cherry, Prunus padus, and the common European plum, P. domestica, in Poland. Detailed male, female and dispersal juvenile morphology and morphometrics are documented and compared with the original description. From partial 18S, ITS1/2 and partial 28S D2-D3 sequences and morphological features (three lateral lines, position of caudal papillae, shape of the delicate spicules with dorsally bent condylus, lack of vulval flap, a long post-uterine branch and a hyaline region at the female tail tip) it can be concluded that B. eucarpus belongs to the eremus group of Bursaphelenchus. The differentiation from related species of the eremus group is discussed.

Two new insect-associated tylenchids, Sychnotylenchus hibisci n. sp. and Neoditylenchus xiphydriae n. sp. (Tylenchomorpha: Anguinidae), from Japan

Nematology ◽  
2021 ◽  
pp. 1-26
Author(s):  
Natsumi Kanzaki ◽  
Hisashi Kajimura ◽  
Hayato Masuya ◽  
Mitsuteru Akiba ◽  
Taisuke Ekino ◽  
...  
Keyword(s):  
New Species ◽  
Bark Beetle ◽  
Body Shape ◽  
Length Ratio ◽  
Male And Female ◽  
Male Tail ◽  
Spicule Length ◽  
Female Tail ◽  
Tail Shape ◽  
Tail Tip

Summary We isolated two tylenchid species belonging to the Sychnotylenchinae, Sychnotylenchus hibisci n. sp. and Neoditylenchus xiphydriae n. sp., from the bark beetle Ernoporus corpulentus and wood wasp Xiphydria ogasawarai, respectively. Sychnotylenchus hibisci n. sp. is characterised by its moderately stout body in both male and female, gubernaculum to spicule length ratio of one-third or less, crustaformeria structure composed of four columns with six large, rounded cells, and a broad female tail with a rounded terminus. The new species is closely related to S. ulmi, with which it shares body shape and gubernaculum to spicule length ratio, but is distinguished by the female tail shape and several morphometric values. Neoditylenchus xiphydriae n. sp. is characterised by its oval to leaf-shaped male bursa, male tail tip reaching the distal end of, and protruding beyond the bursa, a long post-vulval uterine sac (PUS), and a conical female tail with a narrowly rounded terminus. The species is typologically similar to N. pinophilus but is distinguished by its male spicule shape, gubernaculum to spicule length ratio, and longer PUS. Phylogenetically, the Sychnotylenchinae belongs under the Anguinidae (superfamily Sphaerularioidea) and is closely related to several Ditylenchus, Neotylenchus, Neomisticius, and Ficotylus species.

ATTRACTION OF HYLOBIUS PALES (HERBST) (COLEOPTERA: CURCULIONIDAE) TO PHEROMONES OF BARK BEETLES (COLEOPTERA: SCOLYTIDAE)

1990 ◽  
Vol 122 (3) ◽  
pp. 423-427 ◽  
Author(s):  
Thomas W. Phillips
Keyword(s):  
Field Experiment ◽  
Bark Beetle ◽  
Bark Beetles ◽  
Suitable Host ◽  
Male And Female ◽  
Pine Weevil ◽  
Ips Calligraphus ◽  
Hylobius Pales ◽  
Host Resource

AbstractResults of a field experiment indicate that adults of the pine weevil Hylobius pales (Herbst) respond to pheromones of bark beetles. Each sex of H. pales was more attracted to traps baited with the combination of a pine bolt infested with male Ips calligraphus Germar plus the synthetic Dendroctonus Erichson pheromones frontalin and exo-brevicomin, than to traps baited with pine bolts alone. The combined numbers of male and female H. pales caught in traps baited only with Ips calligraphus-infested bolts were significantly greater than numbers caught in traps baited with uninfested control bolts. The attraction of H. pales to bark beetle pheromones may represent a kairomonal response in which weevils exploit semiochemicals from other species that signify a suitable host resource.

Bursaphelenchus chengi sp. n. (Nematoda: Parasitaphelenchidae) isolated at Nanjing, China, in packaging wood from Taiwan

Nematology ◽  
2008 ◽  
Vol 10 (3) ◽  
pp. 335-346 ◽  
Author(s):  
Hongmei Li ◽  
Phap Quang Trinh ◽  
Lieven Waeyenberge ◽  
Maurice Moens
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Body Size ◽  
Posterior Margin ◽  
Petri Dish ◽  
Packaging Materials ◽  
Lateral Field ◽  
Female Tail ◽  
Domain Sequence ◽  
Lateral Lines

Abstract Bursaphelenchus chengi sp. n. is described and illustrated. Dauer juveniles were isolated from imported wood packaging materials from Taiwan to Nanjing Port, China. Bursaphelenchus chengi sp. n. was reared and maintained on Petri dish cultures of the fungus Botrytis cinerea. The new species is characterised by the medium body size in both sexes, the presence of only two incisures in the lateral field and the robust and strongly curved spicules. The spicule lamina is angular distally, the rostrum digitate and the condylus rounded. The tail is arcuate with a pointed terminus. The bursa is usually truncate with the posterior margin indented in some specimens or rounded with a fine axial point. Females have a small vulval flap formed by a short extension of the cuticle of the anterior lip, and a conical tail that gradually tapers to an almost straight or slightly recurved, pointed or rounded terminus. Because of the presence of two lateral lines, similar spicule shape, tapering female tail and the presence of a small vulval flap, B. chengi sp. n. should be grouped in the abietinus-group sensu Braasch. together with B. abietinus, B. antoniae, B. hellenicus, B. hylobianum and B. rainulfi. ITS-RFLP profiles support the proposal of the new species, and phylogenetic analysis of the 28S rDNA D2/D3 domain sequence places it close to B. antoniae and other species of the abietinus-group.

Response of Dryocoetes confusus and D. autographus (Coleoptera: Scolytidae) to enantiospecific pheromone baits

2004 ◽  
Vol 136 (3) ◽  
pp. 419-425 ◽  
Author(s):  
N.L. Jeans Williams ◽  
J.H. Borden
Keyword(s):  
Bark Beetle ◽  
Aggregation Pheromone ◽  
Bark Beetles ◽  
Field Studies ◽  
Pheromone Component ◽  
Male And Female ◽  
Dryocoetes Confusus

AbstractIn past field studies, the greatest response of western balsam bark beetles, Dryocoetes confusus Swaine, to traps was obtained with blends of (+)-exo-brevicomin and (+)- or (±)-endo-brevicomin, which imitate the natural male-produced aggregation pheromone. We conducted a trapping experiment comparing low-release enanti ospecific blends (9:1 (+)-exo-brevicomin:(+)-endo-brevicomin or 9:2 (+)-exo-brevicomin:(±)-endo-brevicomin released at 0.3, 0.1, or 0.03 mg per day) with the standard commercial (±)-exo-brevicomin bait released at 1.2 mg per day. Multiple-funnel traps baited with the experimental blends caught more D. confusus than the unbaited traps, but only traps with the 9:2 (+):(±) blend released at 0.3 and 0.03 mg per day caught significantly more male and female beetles than those baited with the standard bait. Thus, trap sensitivity can be improved with the addition of (±)-endo-brevicomin. The sympatric bark beetle D. autographus Ratzeburg was captured in significant numbers in traps baited with (±)-exo-brevicomin. A subsequent trapping experiment showed that D. autographus responded to (+)- or (±)-exo-brevicomin, but not to (−)-exo-brevicomin, suggesting that (+)-exo-brevicomin is the principal aggregation pheromone component in this species.

Description of Aphelenchoides stellatus n. sp. (Nematoda: Aphelenchoididae) found in packaging wood from Japan

Nematology ◽  
2014 ◽  
Vol 16 (5) ◽  
pp. 621-630 ◽  
Author(s):  
Yiwu Fang ◽  
Jianfeng Gu ◽  
Xuan Wang ◽  
Hongmei Li
Keyword(s):  
New Species ◽  
Excretory Pore ◽  
Phylogenetic Analyses ◽  
Nerve Ring ◽  
Lateral Field ◽  
Male And Female ◽  
Female Tail ◽  
Morphological Status ◽  
Group 3 ◽  
A New Species

Aphelenchoides stellatusn. sp. is described and figured. The new species was isolated from packaging wood from Japan imported to Ningbo harbour, China. The new species has a body length of 485-533 μm (males) and 547-699 μm (females). The cuticle is weakly annulated and there are four lines in the lateral field. The stylet is 9-11 μm long and has small basal swellings. The excretory pore is located posterior to the nerve ring. Spicules smoothly curved, rose-thorn shaped. Apex and rostrum round, only slightly offset, dorsal limb 19-21 μm long. Male tail bearing six (2 + 2 + 2) caudal papillae. The female spermatheca is axial and oblong with round sperm present in multiple rows. Both male and female tail pegs have 3-4 processes, appearing star-like under SEM. The new species belongs to the Group 3 category ofAphelenchoidesspecies. Phylogenetic analyses based on full length ITS and 28S D2/D3 region of rDNA confirmed its morphological status as a new species.

Menzbieria chalcographi, a new neogregarine pathogen of the great spruce bark beetle, Dendroctonus micans (Kugelann) (Curculionidae, Scolytinae)

2012 ◽  
Vol 57 (3) ◽  
Author(s):  
Mustafa Yaman ◽  
Renate Radek
Keyword(s):  
Electron Microscopy ◽  
Biological Control ◽  
Bark Beetle ◽  
Light And Electron Microscopy ◽  
First Record ◽  
Dendroctonus Micans ◽  
Male And Female ◽  
Spruce Bark Beetle ◽  
Spruce Bark ◽  
Life Cycle Stages

AbstractThis study concerns a new neogregarine parasitic in the great spruce bark beetle Dendroctonus micans (Kugelann) (Curculionidae, Scolytinae). The rate of infection was high, reaching 27.3%. There was no difference in the rate of infection of male and female beetles. The life-cycle stages of the pathogen were described by light and electron microscopy. Each gametocyst of the neogregarine included 8–16 actinocephalid oocysts measuring 11.19 ± 0.42 × 4.99 ± 0.25 μm. The described pathogen has the typical characteristics of members of the genus Menzbieria within the order Neogregarinida and it was identified as Menzbieria chalcographi. This is the first record of an infection of D. micans by M. chalcographi. Possibly, this pathogen could be useful for the biological control of this destructive bark beetle.

Description of Pseudaphelenchus zhoushanensis n. sp. (Tylenchina: Aphelenchoididae) found in the wood of Pinus thunbergii at Zhoushan Islands, Zhejiang Province, China

Nematology ◽  
2016 ◽  
Vol 18 (10) ◽  
pp. 1151-1164 ◽  
Author(s):  
Yiwu Fang ◽  
Hongmei Li ◽  
Munawar Maria ◽  
Wim Bert
Keyword(s):  
Distal Part ◽  
Phylogenetic Analyses ◽  
Pinus Thunbergii ◽  
Zhejiang Province ◽  
Female Tail ◽  
Lateral Lines ◽  
The Status ◽  
Molecular Phylogenetic ◽  
Pointed End ◽  
A New Species

Pseudaphelenchus zhoushanensis n. sp. was isolated from a dead Pinus thunbergii at Changgang Mountain, Zhoushan Islands, Zhejiang Province, China. It is characterised by the small to medium length body, cuticle slightly annulated, presence of three lateral lines, stylet 9.0-10.7 μm with small but conspicuous basal knobs, excretory pore located from same level as the metacorpus to slightly anterior to metacorpus, true bursa surrounding entire tail but inconspicuous, male tail conical with a single mucron, spicule with distinct condylus and rostrum strongly arcuate to a pointed end, female tail conical with annulation, strongly ventrally bent in distal part of tail, with terminus bluntly pointed or finely mucronate. Phylogenetic analyses using sequences of the18S and 28S D2-D3 regions of rDNA confirmed the status of P. zhoushanensis n. sp. as a new species. Combining the molecular phylogenetic analyses, morphology and biology of P. zhoushanensis n. sp. and Tylaphelenchus jiaae indicates that T. jiaae is a member of Pseudaphelenchus to which it is herein transferred as P. jiaae n. comb. (= T. jiaae).

Bursaphelenchus saudi n. sp. (Tylenchina: Aphelenchoididae) found in packaging wood from Saudi Arabia

Nematology ◽  
2016 ◽  
Vol 18 (4) ◽  
pp. 475-488 ◽  
Author(s):  
Jianfeng Gu ◽  
Munawar Maria ◽  
Yiwu Fang ◽  
Jie He ◽  
Helen Braasch ◽  
...  
Keyword(s):  
Saudi Arabia ◽  
Excretory Pore ◽  
Gene Sequencing ◽  
Nerve Ring ◽  
Precloacal Papilla ◽  
Rdna Gene ◽  
Female Tail ◽  
Lateral Lines ◽  
Partial 18S

Bursaphelenchus saudi n. sp., isolated in Ningbo, P.R. China, from Pinus packaging wood imported from Saudi Arabia, is described. It is characterised by four lateral lines, excretory pore located between metacorpus and nerve ring (anterior to median bulb before cultured), presence of a thick vulval flap, post-uterine branch ca two-thirds of the vulva-anus distance long, female tail hook-like (slightly ventrally bent before cultured), long and conical, tapering to a bluntly rounded terminus, spicules transversely striated with weakly developed rostrum and condylus, a single precloacal papilla and three pairs of ventro-sublateral papillae (one pair precloacal, two pairs postcloacal just anterior to bursal flap and adjacent to each other). It belongs to the africanus-group of the genus Bursaphelenchus and is most close to B. paraburgeri, B. burgermeisteri and B. obeche. It is distinguished from other Bursaphelenchus species by morphology, ITS-RFLP patterns and partial 18S, ITS and 28S D2-D3 rDNA gene sequencing results.

Description of Ruehmaphelenchus digitulus sp. n. (Nematoda: Aphelenchoididae) found in packaging wood from Taiwan

Nematology ◽  
2012 ◽  
Vol 14 (4) ◽  
pp. 489-498 ◽  
Author(s):  
Jianfeng Gu ◽  
Jiangling Wang
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Rflp Pattern ◽  
Species Status ◽  
Separate Species ◽  
Lateral Field ◽  
Male And Female ◽  
Female Tail ◽  
Molecular Phylogenetic ◽  
Shape And Size

Ruehmaphelenchus digitulus sp. n., isolated from coniferous packaging wood from Taiwan, is described and figured. The new species is characterised by a relatively stout body with a = 28.0 (23.7-31.3) and 27.0 (22.9-32.7) for male and female, respectively, three lines in the lateral field, spicules relatively small (chord = 17.0-19.2 μm; arc = 13.8-16.6 μm) with weakly developed condylus and rostrum, bursa absent, vulva positioned at ca 80% of body length, vulva flap absent, vulval lips slightly protruding, post-uterine branch extending slightly over half of vulva to anus distance, and tail conoid, ca 2-3 anal body diam. long, with terminal process 7-10 μm long. The new species is the fourth known for the genus. It can be distinguished from R. martinii, R. asiaticus and R. thailandae mainly by the different shape and size of the spicules and the different shape and length of the female tail terminus. The separate species status is also supported by ITS-RFLP pattern and molecular phylogenetic analysis based on partial SSU ITS1/2 and partial LSU sequences.

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