Molecular phylogenetic analysis and comparative morphology reveals the diversity and distribution of needle nematodes of the genus Longidorus (Dorylaimida: Longidoridae) from Spain

The genus Longidorus constitutes a large group of approximately 170 species of plant-ectoparasitic nematodes that are polyphagous and distributed almost worldwide. Some of the species of this genus are vectors of plant viruses. Species discrimination in Longidorus is difficult because the morphology is very conservative, and morphometric characters often overlap, leading to potential misidentification. Integrative taxonomy, based on the combination of molecular analyses with morphology, is a useful and necessary approach in Longidorus species identification. In Spain from 2014 to 2017, we conducted nematode surveys among cultivated and wild plants, from which we identified 13 populations of Longidorus, two of which appeared to represent new species and are described herein as L. iliturgiensis sp. nov. and L. pacensis sp. nov., and 11 populations belonging to eight known species: L. africanus, L. baeticus, L. carpetanensis, L. fasciatus, L. nevesi, L. cf. olegi, L. pini, and L. vallensis. Three species are new geographical records for Spain (L. nevesi, L. cf. olegi, and L. africanus). We report molecular data for L. nevesi, L. cf. olegi, L. carpetanensis and L. pini for the first time. Additionally, we describe the males of L. pini and the juveniles of L. cf. olegi.

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Molecular phylogenetic analysis and comparative morphology resolve two new species of olive-tree soil related dagger nematodes of the genus Xiphinema (Dorylaimida : Longidoridae) from Spain

Invertebrate Systematics ◽

10.1071/is16002 ◽

2016 ◽

Vol 30 (6) ◽

pp. 547 ◽

Cited By ~ 8

Author(s):

Antonio Archidona-Yuste ◽

Juan A. Navas-Cortés ◽

Carolina Cantalapiedra-Navarrete ◽

Juan E. Palomares-Rius ◽

Pablo Castillo

Keyword(s):

New Species ◽

Iberian Peninsula ◽

Olive Tree ◽

Comparative Morphology ◽

Integrative Taxonomy ◽

Species Discrimination ◽

Root Cells ◽

Two New Species ◽

Molecular Phylogenetic ◽

Olive Trees

The genus Xiphinema constitutes a large group of ~275 species of polyphagous, plant-ectoparasitic nematodes that are distributed almost worldwide. Some species affect agricultural crops by feeding directly on root cells as well as by transmitting nepoviruses. Despite their agricultural importance, species discrimination in Xiphinema is difficult, leading to potential misidentification. Integrative taxonomy, based on the combination of molecular analyses and morphology, constitutes a new insight into Xiphinema species identification. In this study we describe two new species of Xiphinema from the Iberian Peninsula (X. macrodora, sp. nov. and X. oleae, sp. nov.) associated with cultivated and wild olive trees. Both species have specific rRNA sequences. Morphologically, Xiphinema macrodora, sp. nov. is characterised by a very long body (7.2–8.7 mm), a very long odontostyle and odontophore (190–206 and 105–120 µm, respectively), and a well developed pseudo-Z-organ, comprising 8 to 12 sclerotised bodies. Xiphinema oleae, sp. nov. is characterised by an odontostyle and an odontophore 136–149 and 65–80 µm long, respectively, and a well developed Z-organ with refractive inclusions (3–5), variable in shape. Additionally, X. macrodora, sp. nov. has the longest body size, and the longest odontostyle and odontophore of any Xiphinema, whereas X. oleae, sp. nov. is the first species with a well developed Z-organ from the Iberian Peninsula. http://zoobank.org/urn:lsid:zoobank.org:pub:E4CE8711-22EF-40E4-9D39-6FE962F25225

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Filling missing links in albuneid crab distributions (superfamily Hippoidea) in the Bay of Bengal, eastern Indian Ocean

Crustaceana ◽

10.1163/15685403-00003846 ◽

2018 ◽

Vol 91 (12) ◽

pp. 1495-1504 ◽

Cited By ~ 2

Author(s):

Rajan Kumar ◽

Shikha Rahangdale ◽

Subal Kumar Roul

Keyword(s):

Indian Ocean ◽

Bay Of Bengal ◽

Morphological Characters ◽

Eastern Indian Ocean ◽

Species Discrimination ◽

Gulf Of Mannar ◽

Fishing Gear ◽

Morphometric Characters ◽

First Time

Abstract Albuneid crabs are specialized and active sand-burrowing organisms. Despite their substantial diversity, their ability to avoid fishing gear leads to “under collection” and a discontinuous record of distribution. The present study documents the first distributional record of Albunea occulta Boyko, 2002 from the Bay of Bengal, eastern Indian Ocean. Albunea thurstoni Henderson, 1893 is also recorded for the first time from the area, i.e., from the Gulf of Mannar, southwestern Bay of Bengal. This study further reports variation in morphological characters, especially in the carapace grooves (CG) from previous records and across specimens from different regions. Morphometric characters were found useful in species discrimination, which is explained as well.

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Integrative taxonomy of the Asian skinks Sphenomorphus stellatus (Boulenger, 1900) and S. praesignis (Boulenger, 1900) with the resurrection of S. annamiticus (Boettger, 1901) and the description of a new species from Cambodia

Zootaxa ◽

10.11646/zootaxa.4683.3.4 ◽

2019 ◽

Vol 4683 (3) ◽

pp. 381-411 ◽

Cited By ~ 2

Author(s):

L. LEE GRISMER ◽

PERRY L., JR. WOOD ◽

EVAN S. H. QUAH ◽

SHAHRUL ANUAR ◽

NIKOLAY A. POYARKOV ◽

...

Keyword(s):

New Species ◽

Phylogenetic Analyses ◽

Molecular Data ◽

Color Pattern ◽

Integrative Taxonomy ◽

Peninsular Malaysia ◽

Wildlife Sanctuary ◽

Molecular Phylogenetic ◽

Univariate Analyses ◽

A New Species

Molecular phylogenetic analyses of the sister species Sphenomorphus stellatus and S. praesignis based on the mitochondrial genes 12S and 16S rRNA recover the former as paraphyletic with respect to the latter in that a specimen of S. stellatus from the type locality in Peninsular Malaysia is more closely related to S. praesignis than to Indochinese populations of S. stellatus. Furthermore, the phylogeny indicates that the Indochinese populations represent two species, thus resulting in four major lineages within this clade. These relationships are consistent with multivariate and univariate analyses of morphological and discrete color pattern data which statistically define and diagnose the four lineages and together with the molecular data, provide the foundation for robust, testable, species-level hypotheses. As such, S. stellatus is herein restricted to Peninsular Malaysia; S. annamiticus is resurrected for the circum-continental populations ranging through southeastern Thailand, southern Cambodia, and southern Vietnam; a new species—S. preylangensis sp. nov.—is described from an isolated mountain, Phnom Chi, from the Prey Lang Wildlife Sanctuary in central Cambodia; and the taxonomy of S. praesignis remains unchanged. The description of S. preylangensis sp. nov. underscores the necessity to conserve this remnant of lowland evergreen rainforest in the Prey Lang Wildlife Sanctuary.

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High diversity in the Pseudechiniscus suillus–facettalis complex (Heterotardigrada: Echiniscidae) with remarks on the morphology of the genus Pseudechiniscus

Zoological Journal of the Linnean Society ◽

10.1093/zoolinnean/zlz171 ◽

2020 ◽

Vol 188 (3) ◽

pp. 733-752 ◽

Cited By ~ 6

Author(s):

Daria Grobys ◽

Milena Roszkowska ◽

Magdalena Gawlak ◽

Hanna Kmita ◽

Andrzej Kepel ◽

...

Keyword(s):

Molecular Data ◽

Integrative Taxonomy ◽

Morphological Features ◽

Species Discrimination ◽

Extensive Discussion ◽

Morphology And Morphometry ◽

Species Specific ◽

High Diversity ◽

General Appearance

Abstract Pseudechiniscus is a morphologically homogeneous genus of tardigrades. The morphological features commonly used for species discrimination in this genus are the dorsal sculpture, the shape and number of dorsal plates and trunk appendages. Species of the Pseudechiniscus suillus–facettalis complex are one of the most challenging tardigrades to identify. All species are similar in their general appearance and all lack trunk appendages. Moreover, not only the nominal Pseudechiniscus suillus, but also other members of the suillus–facettalis complex have been insufficiently described. In our study, we examined several populations from the Northern and the Southern Hemispheres that could be traditionally attributed to Pse. suillus. These populations were analysed using integrative taxonomy – a combination of classical morphology and morphometry with molecular data. Besides the differences in the dorsal sculpture and morphometry, we also found species-specific differences in ventral sculpture, which were originally used for discrimination of Pseudechiniscus species. Moreover, we provide an extensive discussion on all morphological and morphometric differences used in Pseudechiniscus taxonomy and indicate main taxonomic problems with this genus. Finally, we redescribe the nominal Pse. suillus from Italy.

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Additional data on Iranian trichodorids (Triplonchida: Trichodoridae) and first record of a rare species, Trichodorus variabilis Roca, 1998

Nematology ◽

10.1163/15685411-00003036 ◽

2017 ◽

Vol 19 (2) ◽

pp. 121-129 ◽

Cited By ~ 18

Author(s):

Majid Pedram ◽

Ali Roshan-Bakhsh ◽

Ebrahim Pourjam ◽

Mohammad Reza Atighi ◽

Wilfrida Decraemer ◽

...

Keyword(s):

Phylogenetic Analyses ◽

First Record ◽

Molecular Data ◽

Slight Variation ◽

Natural Forests ◽

Northern Iran ◽

Phylogenetic Studies ◽

Molecular Phylogenetic ◽

First Time ◽

Of Greece

Trichodorus variabilis, recovered from three separate locations in natural forests of northern Iran, was studied using morphological, morphometric and molecular data. Variation in position of the ventromedian cervical papillae (CP1 and CP2) with respect to the onchiostyle base in the resting position, and spicule characters (having or lacking striation in distal blade region and bristles in proximal blade region) were observed. Variation was also observed in the nature of the pharyngo-intestinal junction (offset to slight overlapping). The secretory-excretory pore of females also showed slight variation in placement. Molecular phylogenetic studies, using partial 28S rDNA D2-D3 sequences of three studied populations and one sequenced isolate of the species from Greece, revealed T. variabilis has variation in the sequences of this genomic fragment. The similarity percent of four sequences ranged from 96.7 to 99.7%. The species was found for the first time outside of Greece, the country from which it was originally described. A newly recovered population of T. persicus, originally described from Iran, was also included in the molecular phylogenetic analyses.

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Integrative taxonomy reveals two new cryptic species of Hyphessobrycon Durbin, 1908 (Teleostei: Characidae) from the Maracaçumé and middle Tocantins River basins, Eastern Amazon region

European Journal of Taxonomy ◽

10.5852/ejt.2020.723.1145 ◽

2020 ◽

Vol 723 ◽

pp. 77-107

Author(s):

Erick Cristofore Guimarães ◽

Pâmella Silva De Brito ◽

Pedro Henrique Negreiros Bragança ◽

Jadson Pinheiro Santos ◽

Axel Makay Katz ◽

...

Keyword(s):

New Species ◽

Mitochondrial Gene ◽

Morphological Characters ◽

Molecular Data ◽

Color Pattern ◽

Integrative Taxonomy ◽

The Body ◽

Dorsal Fin ◽

Two New Species ◽

Molecular Phylogenetic

Two new species, Hyphessobrycon frickei Guimarães, Brito, Bragança, Katz & Ottoni sp. nov. and H. geryi Guimarães, Brito, Bragança, Katz & Ottoni sp. nov., are herein described, based on seven different and independent species delimitation methods, and on molecular and morphological characters, making the hypothesis of these new species supported from an integrative taxonomy perspective. They belong to the “Rosy tetra” clade, which is mainly characterized by the presence of a dark brown or black blotch on the dorsal fin and the absence of a midlateral stripe on the body. These two new species are distinguished from the other members of this clade mainly by the arrangement, shape and color pattern of humeral and dorsal-fin spots, as well as by other characters related to scale counts and body pigmentation. The placement of the new species within the “Rosy tetra” clade was based on the combination of morphological character states mentioned above and corroborated by a molecular phylogenetic analysis using the mitochondrial gene cytochrome oxidase subunit 1. In addition, a new clade (here termed Hyphessobrycon copelandi clade) within the “Rosy tetra” clade is proposed based on molecular data, comprising H. copelandi, H. frickei sp. nov., H. geryi sp. nov. and a still undescribed species. Our results corroborate the occurrence of hidden species within the “Rosy tetra” clade, as suggested by previous studies.

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Integrative descriptions of two new Macrobiotus species (Tardigrada, Eutardigrada, Macrobiotidae) from Mississippi (USA) and Crete (Greece)

Zoosystematics and Evolution ◽

10.3897/zse.97.65280 ◽

2021 ◽

Vol 97 (1) ◽

pp. 281-306

Author(s):

Matteo Vecchi ◽

Daniel Stec

Keyword(s):

18S Rrna ◽

Molecular Data ◽

Integrative Taxonomy ◽

Distal Portion ◽

Morphological Data ◽

28S Rrna ◽

Morphometric Characters ◽

Clade B ◽

Specific Morphology ◽

Definition Of

In this paper, we describe two new Macrobiotus species from Mississippi (USA) and Crete (Greece) by means of integrative taxonomy. Detailed morphological data from light and scanning electron microscopy, as well as molecular data (sequences of four genetic markers: 18S rRNA, 28S rRNA, ITS-2 and COI), are provided in support of the descriptions of the new species. Macrobiotus annewintersaesp. nov. from Mississippi belongs to the Macrobiotus persimilis complex (Macrobiotus clade B) and exhibits a unique egg processes morphology, similar only to Macrobiotus anemone Meyer, Domingue & Hinton, 2014, but mainly differs from that species by the presence of eyes, granulation on all legs, dentate lunulae on legs IV, and of bubble-like structures within the tentacular arms that are present on the distal portion of the egg processes. Macrobiotus rybakisp. nov. from Crete belongs to the Macrobiotus clade A and is most similar to Macrobiotus dariae Pilato & Bertolani, 2004, Macrobiotus noemiae Roszkowska & Kaczmarek, 2019, Macrobiotus santoroi Pilato & D’Urso, 1976, and Macrobiotus serratus Bertolani, Guidi & Rebecchi, 1996, but differs from them mainly in the morphological details of its egg processes and chorion reticulation, but also by a number of morphometric characters. In light of the specific morphology of the egg processes of Macrobiotus annewintersaesp. nov. and Macrobiotus anemone, that are equipped with tentacular arms instead of proper terminal disc, we also provide an updated definition of the Macrobiotus persimilis complex.

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Molecular phylogeny of several species of Hoplolaimina (Nematoda: Tylenchida) associated with turfgrass in Korea, with comments on their morphology

Nematology ◽

10.1163/15685411-bja10061 ◽

2020 ◽

pp. 1-18

Author(s):

Abraham Okki Mwamula ◽

Gayeong Lee ◽

Yeong Ho Kim ◽

Young Ho Kim ◽

Kwang-Soo Lee ◽

...

Keyword(s):

Phylogenetic Analyses ◽

Integrative Taxonomy ◽

Rrna Genes ◽

28S Rrna ◽

Gene Sequences ◽

Meloidogyne Graminicola ◽

Molecular Phylogenetic ◽

Its Rrna ◽

First Time ◽

Pratylenchus Zeae

Summary Seven species belonging to Suborder Hoplolaimina are characterised using integrative taxonomy, considering both morphological and molecular phylogenetic analyses of the 28S-rRNA, ITS-rRNA and COI gene sequences. It is evident that, as more populations of Pratylenchus zeae are continuously characterised, the species continues to display an ever-increasing intraspecific genetic variation within the 28S-rRNA and ITS-rRNA genes. However, the COI gene sequences exhibit minimum intraspecific variation and thus might be the most powerful DNA barcoding marker for the precise identification of P. zeae and should therefore be recommended as a complementary technique in the identification process of the species. Pratylenchus zeae, Meloidogyne graminicola and Heterodera pratensis are characterised herein for the first time in Korea, while the presence in Korea of P. penetrans, P. scribneri, H. avenae, and M. marylandi, is molecularly confirmed.

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Phylogenetic position of Coronastrum ellipsoideum and description of Parachlorella hussii sp. nov.

Biologia ◽

10.2478/s11756-011-0056-8 ◽

2011 ◽

Vol 66 (4) ◽

Cited By ~ 14

Author(s):

Christina Bock ◽

Marie Pažoutová ◽

Lothar Krienitz

Keyword(s):

Molecular Data ◽

Phylogenetic Position ◽

Rrna Gene ◽

Combined Approach ◽

Molecular Analyses ◽

Phylogenetic Studies ◽

The Family ◽

Its Rrna Gene ◽

Molecular Phylogenetic ◽

First Time

AbstractFollowing traditional morphological concepts, the genus Coronastrum is considered to be a rare member of the Scenedesmaceae (Chorophyceae). This classification may be called into question when molecular data are taken into account as well. Recent molecular phylogenetic studies revealed the polyphyletic origin of the family Scenedesmaceae within the Chlorophyceae and Trebouxiophyceae. In a combined approach of morphological analyses, SSU/ITS rRNA gene phylogeny and comparison of the ITS secondary structure, we analysed the systematics of Coronastrum strains available in public strain collections. Our molecular analyses revealed a new subclade within the Chlorella clade of the Chlorellaceae consisting of Coronastrum ellipsoideum, two strains with Dictyosphaerium-like morphology and one strain which fits the description of the genus Parachlorella. Four additional strains formed together a new lineage within the genus Parachlorella in the Parachlorella clade of the Chlorellaceae. These strains differ from the already known Parachlorella species in complementary base changes within the ITS2 and are here described for the first time as Parachlorella hussii sp. nov.

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The formerly enigmatic Unidentiidae in the limelight again: a new species of the genus Unidentia from Thailand (Gastropoda: Nudibranchia)

Zootaxa ◽

10.11646/zootaxa.4551.5.4 ◽

2019 ◽

Vol 4551 (5) ◽

pp. 556 ◽

Cited By ~ 3

Author(s):

TATIANA KORSHUNOVA ◽

RAHUL MEHROTRA ◽

SPENCER ARNOLD ◽

KENNET LUNDIN ◽

BERNARD PICTON ◽

...

Keyword(s):

New Species ◽

Phylogenetic Analyses ◽

Molecular Data ◽

Morphological Data ◽

Sea Slugs ◽

The Family ◽

Molecular Phylogenetic ◽

Morphological Differences ◽

First Time ◽

A New Species

An integrative molecular and morphological study is presented for the family Unidentiidae. Molecular phylogenetic analyses were conducted with the inclusion of all previous and newly obtained molecular data for the family Unidentiidae Millen & Hermosillo 2012. A new species of the genus Unidentia Millen & Hermosillo 2012, U. aliciae sp. nov., is described from Thailand as part of an inventory of sea slugs at Koh Tao. All up-to-date available morphological data for the species of the genus Unidentia is for the first time summarized. Morphological differences among the different species of Unidentia are clarified showing that every species has its own distinguishable morphological traits. According to the new molecular and morphological data, the family Unidentiidae is re-confirmed as a well-supported taxon of the aeolidacean nudibranchs. The taxonomy and phylogeny of the Aeolidacea in the light of the family Unidentiidae is briefly discussed and necessity of a fine-scale and narrowly-defined taxa approach instead of a ‘‘superlumping’’ one is highlighted.

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