scholarly journals Morphological and Molecular Characterization of Kochinema farodai Baqri and Bohra, 2001 (Dorylaimida: Nordiidae) from California, with the First Molecular Study and an Updated Taxonomy of the Genus

Animals ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 2300
Author(s):  
Sergio Álvarez-Ortega
Keyword(s):  
Molecular Characterization ◽  
Molecular Study ◽  
Recent Common Ancestor ◽  
Integrative Approach ◽  
Distribution Data ◽  
Rrna Gene ◽  
28S Rrna ◽  
The Family ◽  
Morphological And Molecular Characterization

This paper deals with the morphological and molecular characterization of Kochinema farodai Baqri and Bohra, 2001, with an integrative approach. The finding of K. faroidai in California is a remarkable biogeographical novelty, as it is the first American record of the species. Molecular data herein obtained represent the first molecular study of the genus Kochinema. Furthermore, scanning electron microscopy (SEM) observations of a member of Kochinema are provided for the first time. Additionally, this contribution provides new insights into the phylogeny and taxonomy of the nematode genus Kochinema. A brief historical outline of the matter is presented. Then, the morphological pattern of the genus is revised and illustrated, the anterior position of amphids, whose opening is located on lateral lip, being its most relevant diagnostic feature. The phylogenetic analysis inferred from D2–D3 expansion segments of 28S rRNA gene shows that Kochinema clustered together with other dorylaimid species characterized by the absence of pars refringens vaginae and that it does not share a recent common ancestor with other members of the family Nordiidae. A likely polyphyly of the family Nordiidae is confirmed. Finally, an updated taxonomy of the genus is proposed, including a revised diagnosis, a list of species, a key to species identification, and a compendium of their main morphometrics and distribution data.

Morphological characterization and molecular phylogeny of zoonotic trematodes in the freshwater snail Asolene platae

Parasitology ◽  
2019 ◽  
Vol 146 (07) ◽  
pp. 839-848
Author(s):  
Federico A. Dellagnola ◽  
Martín M. Montes ◽  
Sergio R. Martorelli ◽  
Israel A. Vega
Keyword(s):  
Molecular Study ◽  
Freshwater Snail ◽  
Morphological Characterization ◽  
Pomacea Canaliculata ◽  
Rrna Gene ◽  
28S Rrna ◽  
Symbiotic Associations ◽  
The Family ◽  
Molecular Databases ◽  
Amplicon Size

AbstractIn the context of a broader program dealing with the symbiotic associations of apple snails, we sampled three species that coexist in Lake Regatas (Palermo, Buenos Aires, Argentina). The population ofAsolene platae, (but not those ofPomacea canaliculataandPomacea scalaris) showed trematode larvae (echinocercariae and xiphidiocercariae) in haemocoelic spaces and connective tissue of the digestive gland. The echinocercariae resembled those of the genusEchinochasmus, but lacked sensory hairs on their body and tail; whereas xiphidiocercariae were similar to the xiphidiocercariae armatae belonging to the Opisthoglyphe type. The phylogenetical positions of these trematodes were inferred by the 28S rRNA, ITS1 and mtCOXI gene sequences. The 28S rRNA gene linked the echinocercarial sequences with the polyphyletic genusEchinochasmus(Echinochasmidae), while the xiphidiocercarial sequences were linked with the genusPhaneropsolus(Phaneropsolidae). The molecular markers used were able to distinguish two cryptic molecular entities of the single echinocercarial morphotype. Although ITS1 and mtCOXI did not allow resolving phylogeny beyond the family level because of the scarce number of sequences in the molecular databases, both cercariae (echinocercariae and xiphidiocercariae) could be distinguished by the Internal Transcribed Spacer 1 amplicon size. This is the first correlative morphological and molecular study of zoonotic trematodes in Neotropical ampullariids.

scholarly journals Morphological and molecular characterization of Gordionus alpestris (Nematomorpha) from the North-West Caucasus, Russia

2013 ◽  
Vol 50 (1) ◽  
pp. 67-72 ◽  
Author(s):  
S. Spiridonov ◽  
V. Schmatko
Keyword(s):  
Molecular Characterization ◽  
North West ◽  
The North ◽  
The Family ◽  
Pcr Products ◽  
High Level ◽  
Morphological And Molecular Characterization

AbstractDuring the survey of Pachyiulus krivolutskyi Golovatch, 1977 (Diplopoda) near Nickel’ in Adygei Republic (Russia) in the summer 2009 the infestation of these millipeds with nematomorphs of the species Gordionus alpestris was revealed. The morphology of naturally emerging nematomorphs was studied in SEM. The partial sequences of 18S (883 bp) and 28S (393 bp) rDNA PCR-products were obtained and analyzed. An unexpectedly high level of differences between Gordionus alpestris found in diplopods of North-West Caucasus and other molecularly studied representatives of the family Chordodidae was observed.

scholarly journals Morphological and Molecular Characterization of Nothotylenchus medians and N. similis (Nematoda: Anguinidae) from Southern Alberta, Canada

Horticulturae ◽  
2022 ◽  
Vol 8 (1) ◽  
pp. 74
Author(s):  
Maria Munawar ◽  
Atta Ur Rahman ◽  
Pablo Castillo ◽  
Dmytro P. Yevtushenko
Keyword(s):  
Molecular Characterization ◽  
Phylogenetic Analyses ◽  
Original Description ◽  
Distribution Data ◽  
Plant Parts ◽  
Full Diversity ◽  
Canadian Population ◽  
Inventory Survey ◽  
Morphological And Molecular Characterization

The nematode family, Anguinidae, is a diverse group of polyphagous nematodes, generally known as fungal feeders or parasites of aerial plant parts. Here, we present the morphological and molecular characterization of adult females of two Nothotylenchus species, N. medians and N. similis, along with host association and geographical distribution data of the genus. Both species are recorded as new reports from Canada and designated as reference populations for future studies. Morphological or morphometrical variation was not observed in the Canadian population of N. medians and N. similis, in comparison with the original description. Phylogenetic analyses based on 18S and D2–D3 of 28S genes placed both species within Anguinidae. Since the biology of the genus Nothotylenchus has not been rigorously characterized, the habitat and distribution information presented in this study will shed some light on the ecology of these nematodes. Notably, the detection of N. medians and N. similis in our nematode inventory survey indicates that considerable Nothotylenchus diversity is hidden in these soils. Consequently, increased surveys and more in-depth research are needed to explore the full diversity of anguinids inhabiting these cultivated areas.

Morphological and molecular characterization of Verticillium dahliae population occurring in different olive-growing regions of Morocco

2015 ◽  
Vol 4 (6) ◽  
pp. 300-307
Author(s):  
Soumia Darai ◽  
Abdelali Chourfi ◽  
Mohammed Diouri ◽  
Said Amiri
Keyword(s):  
Molecular Characterization ◽  
Verticillium Dahliae ◽  
Molecular Study ◽  
Molecular Approach ◽  
Specific Primers ◽  
Morphological And Molecular Characterization

  In order to characterize the V. dahliae population occurring in different olive-growing regions of Morocco, a morphological and molecular study was con-ducted. According to pigmentation and appearance of the mycelium, isolates were classified into three morphotypes. The dimensions length (leng) and width (lar) of conidia showed no significant differences between isolates. During molecular confirmation of the isolates identity, we found a polymor-phism linked to the origin of extracted DNA (mycelium or microsclerotia cell). The molecular approach based on the use of three specific primers for amplification of the 32 isolates’genomic DNA , allowed us to divide the fun-gal collection into two groups defoliating and non-defoliating. The DNA ori-gin and the degree of pathogenicity of the isolate appeared to be respon-sible for this polymorphism. Therefore this study indicates a correlation bet-ween the pathotype and pathogenicity of the fungus.

Morphological and molecular characterization of Pratylenchoides persicus n. sp. (Nematoda: Merliniidae) and additional data on two other species of the genus from Iran

Zootaxa ◽  
2016 ◽  
Vol 4205 (5) ◽  
pp. 459 ◽  
Author(s):  
KOUROSH AZIZI ◽  
ALI ESKANDARI ◽  
AKBAR KAREGAR ◽  
REZA GHADERI ◽  
SVEN VAN DEN ELSEN ◽  
...  
Keyword(s):  
New Species ◽  
Large Subunit ◽  
Molecular Study ◽  
Closely Related Species ◽  
The Family ◽  
Lesion Nematode ◽  
Stylet Length ◽  
First Time ◽  
Morphological And Molecular Characterization

Some nematologists recently placed the genus Pratylenchoides, (“Lesion Nematode-like”) in the family Merliniidae. To investigate Pratylenchoides species diversity and their relationships with other Merliniidae genera, specimens were collected from various habitats in the northern and northwestern provinces of Iran. The morphological and molecular study yielded three species of the genus Pratylenchoides, including P. persicus n. sp. This new species is characterized by having lip region rounded or slightly flattened anteriorly with four or five fine but distinct annuli, pharyngeal glands off-set or slightly overlapping the intestine dorsally, all three gland nuclei located anterior to the pharyngo-intestinal valve and tail cylindrical with truncate to low rounded terminus. Morphologically, P. persicus n. sp. can be distinguished from the most closely related species, P. heathi by having shorter body and stylet length in females and males, as well as a shorter tail with different terminus in females. Pratylenchoides laticauda and P. cf. nevadensis are reported from Iran for the first time. Phylogenetic analysis based on the D2/D3 region of the large subunit of ribosomal DNA revealed Pratylenchoides as a monophyletic genus, and it supports the delineation of the new species, P. persicus n. sp. 

scholarly journals Morphological and Molecular Characterization of Brasilonema roberti-lamii (Cyanophyceae, Nostocales, Scytonemataceae), from Central Mexico

Phytotaxa ◽  
2014 ◽  
Vol 164 (4) ◽  
pp. 255 ◽  
Author(s):  
BEATRIZ RODARTE ◽  
ITZEL BECERRA-ABSALÓN ◽  
GUSTAVO A. MONTEJANO ◽  
KARINA OSORIO-SANTOS ◽  
LUISA ALBA-LOIS ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Growth Form ◽  
Good Alternative ◽  
Molecular Data ◽  
Central Mexico ◽  
Rrna Gene ◽  
Morphological Criteria ◽  
Ecological Criteria ◽  
Morphological And Molecular Characterization

This paper is a contribution to the morphological and molecular characterization of the cyanobacterium Brasilonema roberti-lamii from populations found in Central Mexico. The general growth form and the morphological, morphometric and ecological characteristics of the populations studied clearly correspond to those described for Brasilonema roberti-lamii (basionym: Tolypothrix roberti-lamii) from the French Antilles. Based on molecular data from DNA sequencing of the16S rRNA gene and the IGS of the cpcB-cpcA phycocyanin operon (cpcBA-IGS), we propose that the populations that we studied are closely related to those of other Brasilonema species, including B. octagenarum UFV-OR1, UFV-E1 and HA4187-MV1-p1F, Brasilonema sennae CENA 114, B. tolantongensis, B. terrestre CENA 116, B. angustatum HA4187-MV1-B2+p1F and HA4187-MV1-B2+p1H and B. bromeliae SPC951. Our findings support the transference of Tolypothrix roberti-lamii, which was made based exclusively on morphological criteria, to Brasilonema. The use of molecular analyses in addition to traditional morphological and ecological criteria, known as polyphasic approach, is a good alternative to describe taxa of cyanobacteria, mainly at the genus and species levels. 

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