Two new green algae, Rhizoclonium fractum sp. nov. and R. umbraticum sp. nov., from tropical and subtropical brackish waters of Japan

Phytotaxa ◽  
2016 ◽  
Vol 266 (4) ◽  
pp. 231 ◽  
Author(s):  
KENSUKE ICHIHARA ◽  
SATOSHI SHIMADA ◽  
KAZUYUKI MIYAJI
Keyword(s):  
Ribosomal Rna ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Morphological Characteristics ◽  
Taxonomic Status ◽  
Small Subunit ◽  
Mangrove Forests ◽  
Brackish Waters ◽  
Diagnostic Characteristics ◽  
Molecular Phylogenetic

Microfilamentous unbranched cladophoralean algae are distributed from high latitudes to the tropics in waters of varying salinities. Their simple morphology provides few diagnostic characteristics for distinguishing species, such that the taxonomic status of minute Cladophoraceae remains unclear. In a recent field survey in Japanese mangrove forests and brackish waters, we found Rhizoclonium-like cladophoralean algae (Cladophoraceae spp. 1–3). In molecular phylogenetic analyses based on partial small subunit ribosomal RNA (SSU) and large subunit ribosomal RNA (LSU) genes, these algae clustered with R. africanum Kützing in the LSU tree and were separated from the true Rhizoclonium clade in both the LSU and SSU trees. An early divergence in the Cladophoraceae lineage was also strongly supported in both trees. Cladophoraceae spp. 1–3 and R. africanum shared a specific rhizoidal developmental structure, the “knee-like joint.” Cladophoraceae spp. 1–3 also shared polypyramidal pyrenoids, while typical bilenticular pyrenoids are hardly found. Cladophoraceae sp. 1 is characterized by wider cells and more nuclei per cell than described taxa. Cladophoraceae sp. 3 had a smaller cell length/diameter ratio and fewer nuclei per cell than described taxa. Based on these distinct morphological features, we describe two new species: R. fractum Ichihara et Miyaji sp. nov. and R. umbraticum Ichihara et Miyaji sp. nov. We also revealed that Cladophoraceae sp. 2 should be assigned to the described species R. minutissimum (Zeller) P. C. Silva based on morphological characteristics.

scholarly journals Multi-Gene Phylogeny and Taxonomy of Hydnellum (Bankeraceae, Basidiomycota) from China

2021 ◽  
Vol 7 (10) ◽  
pp. 818
Author(s):  
Yan-Hong Mu ◽  
Jia-Rui Yu ◽  
Ting Cao ◽  
Xiang-Hua Wang ◽  
Hai-Sheng Yuan
Keyword(s):  
New Species ◽  
Rna Polymerase Ii ◽  
Ribosomal Rna ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Morphological Characteristics ◽  
Small Subunit ◽  
Phylogenetic Position ◽  
Ribosomal Rna Gene ◽  
Morphological Studies

The genus Hydnellum is an important group of stipitate hydnaceous fungi which can form ectomycorrhiza with many species of woody plants. In recent decades, the frequency and number of basidiocarps observed in China have been declining significantly. So far, however, we know little about the species diversity of Hydnellum in China. In this study, we conducted molecular phylogenetic analyses based on sections of multiple loci, including the large subunit of nuclear ribosomal RNA gene (nLSU), the internal transcribed spacer regions (ITS), the small subunit of nuclear ribosomal RNA gene (SSU) and the second-largest subunit of RNA polymerase II gene (RPB2), as well as morphological studies, of collected samples of Hydnellum from China. We also inferred Maximum Likelihood and Bayesian phylogenies for the order Thelephorales from the dataset of the combined nLSU and ITS. This study has revealed the phylogenetic position of Hydnellum in the order Thelephorales, and phylogenetically confirmed ten major clades in Thelephorales; Twenty-nine taxa are proposed, described or reported, including 10 new subgenera (Hydnellum subgenus Hydnellum, subg. Caesispinosum, subg. Croceum, subg. Inflatum, subg. Rhizomorphum, subg. Scabrosum, subg. Spongiosum, subg. Subindufibulatum, subg. Violaceum and subg. Zonatum), 11 new species (Hydnellum atrorubrum, H. atrospinosum, H. bomiense, H. brunneorubrum, H. fibulatum, H. granulosum, H. inflatum, H. rubidofuscum, H. squamulosum, H. sulcatum and H. yunnanense), 3 newly recorded species (H. caeruleum, H. peckii and H. spongiosipes) and 5 notable specimens (Hydnellum sp 1, H. sp 2, H. sp 3, H. sp 4 and H. sp 5). A classification system based on the morphological characteristics (especially the hyphal structure types) and molecular analyses is proposed to accommodate most species in Hydnellum. The distinguishing characters of the subgenera and the new species with their closely related taxa are discussed. A key to the species of Hydnellum from China is provided.

Trechispora yunnanensis sp. nov. (Hydnodontaceae, Basidiomycota) from China

Phytotaxa ◽  
2019 ◽  
Vol 424 (4) ◽  
pp. 253-261 ◽  
Author(s):  
TAI-MIN XU ◽  
YU-HUI CHEN ◽  
CHANG-LIN ZHAO
Keyword(s):  
Ribosomal Rna ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Strong Support ◽  
Morphological Characteristics ◽  
Molecular Data ◽  
Fungal Species ◽  
Monophyletic Lineage ◽  
Molecular Phylogenetic ◽  
Inference Methods

A new wood-inhabiting fungal species, Trechispora yunnanensis sp. nov., is proposed based on morphological characteristics and molecular phylogenetic analyses. The species is characterized by resupinate basidiomata, rigid and fragile up on drying, cream to pale greyish hymenial surface; a monomitic hyphal system with generative hyphae bearing clamp connections, IKI-, CB-; ellipsoid, hyaline, thick-walled, ornamented, IKI-, CB- basidiospores measuring as 7–8.5 × 5–5.5 µm. The internal transcribed spacer (ITS) and the large subunit (LSU) regions of nuclear ribosomal RNA gene sequences of the studied samples were generated, and phylogenetic analyses were performed with maximum likelihood (ML), maximum parsimony (MP) and bayesian inference methods (BPP). The phylogenetic analyses based on molecular data of ITS+nLSU sequences showed that T. yunnanensis formed a monophyletic lineage with a strong support (100% ML, 100% MP, 1.00 BPP) and was closely related to T. byssinella and T. laevis. Both morphological characteristics and results of molecular phylogenetic analyses confirmed the placement of the new species in Trechispora.

scholarly journals Phylogeny and diversity of Haploporus (Polyporaceae, Basidiomycota)

MycoKeys ◽  
2019 ◽  
Vol 54 ◽  
pp. 77-98 ◽  
Author(s):  
Meng Zhou ◽  
Li Wang ◽  
Tom W. May ◽  
Josef Vlasák ◽  
Jia-Jia Chen ◽  
...  
Keyword(s):  
New Zealand ◽  
Ribosomal Rna ◽  
Internal Transcribed Spacer ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Morphological Characteristics ◽  
New Combination ◽  
Ribosomal Rna Gene ◽  
Molecular Phylogenetic ◽  
The Usa

Four species of Haploporus, H.angustisporus, H.crassus, H.gilbertsonii and H.microsporus are described as new and H.pirongia is proposed as a new combination, based on morphological characteristics and molecular phylogenetic analyses inferred from internal transcribed spacer (ITS) and large subunit nuclear ribosomal RNA gene (nLSU) sequences. Haploporusangustisporus, H.crassus and H.microsporus occur in China, H.gilbertsonii occurs in the USA, and the distribution of H.pirongia is extended from New Zealand to Australia. Haploporusangustisporus is characterized by the distinct narrow oblong basidiospores measuring 10.5–13.5 × 3.9–5 µm. Haploporuscrassus is characterized by the presence of ventricose cystidioles occasionally with a simple septum, dissepimental hyphae usually with a simple septum, unique thick-walled basidia and distinctly wide oblong basidiospores measuring 13.5–16.5 × 7.5–9.5 µm. Haploporusgilbertsonii is characterized by its large pores (2–3 per mm), a dimitic hyphal structure with non-dextrinoid skeletal hyphae and wide oblong basidiospores measuring 12–15 × 6–8 µm. Haploporusmicrosporus is characterized by distinctly small pores (7–9 per mm), the presence of dendrohyphidia, and distinctly small ellipsoid basidiospores measuring 5.3–6.7 × 3–4.1 µm. Haploporuspirongia is proposed as a new combination. Haploporusamarus is shown to be a synonym of H.odorus and Pachykytosporawasseri is considered a synonym of H.subtrameteus.

scholarly journals Molecular Phylogeny and Global Diversity of the Genus Haploporus (Polyporales, Basidiomycota)

2021 ◽  
Vol 7 (2) ◽  
pp. 96
Author(s):  
Meng Zhou ◽  
Yu-Cheng Dai ◽  
Josef Vlasák ◽  
Yuan Yuan
Keyword(s):  
Ribosomal Rna ◽  
Internal Transcribed Spacer ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Small Subunit ◽  
Geographic Range ◽  
Rrna Gene ◽  
Pore Surface ◽  
Mitochondrial Rrna ◽  
Molecular Phylogenetic

Phylogeny and taxonomy of the genus Haploporus were carried out based on a larger number of samples covering a wider geographic range including East Asia, South Asia, Europe, and America, and the species diversity of the genus is updated. Four species, Haploporus bicolor, H. longisporus, H. punctatus and H. srilankensis, are described as new species based on morphology and molecular phylogenetic analyses inferred from the internal transcribed spacer (ITS), the large subunit nuclear ribosomal RNA gene (nLSU), and the small subunit mitochondrial rRNA gene (mtSSU). Haploporus bicolor is characterized by the distinctly different colors between the pore surface and the tubes, small pores measuring 5–7 per mm, and narrow basidiospores measuring 10.5–11.9 × 4.5–5 µm; H. longisporus differs from other species in the genus by its large pores measuring 2–3 per mm, hyphae at dissepiment edge with simple septum, and the long basidiospores (up to 22 µm); H. punctatus is distinguished by its cushion-shaped basidiocarps, wide fusiform cystidioles with a simple septum at the tips, the absence of dendrohyphidia and the cylindrical to slightly allantoid basidiospores measuring 9–10.8 × 3.8–5 µm; H. srilankensis is characterized by its perennial habit, small pores measuring 4–5 per mm, dextrinoid skeletal hyphae, the presence of cystidioles and dendrohyphidia. An identification key to accepted species of Haploporus is provided.

scholarly journals New Species of Tomentella (Thelephorales, Basidiomycota) from Temperate Continental Mountain Climate of China (Xinjiang Region)

Forests ◽  
2021 ◽  
Vol 12 (11) ◽  
pp. 1531
Author(s):  
Xu Lu ◽  
Haisheng Yuan
Keyword(s):  
New Species ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Morphological Characteristics ◽  
Northwest China ◽  
Tropical Regions ◽  
Mountain Climate ◽  
Molecular Phylogenetic ◽  
Three New Species ◽  
Xinjiang Autonomous Region

Species in the genus Tomentella are distributed throughout the temperate and tropical regions worldwide, but few studies associated with the taxonomy and phylogeny of this genus had been reported from Northwest China. In this paper, molecular phylogenetic analyses of the nuclear ribosomal ITS (internal transcribed spacer: ITS1-5.8S-ITS2) and LSU (large subunit: 28S) sequences combined with morphological characteristics identified three new species from Xinjiang Autonomous Region in Northwest China, which were named T. aurantispora, T. kanasensis, and T. schrenkiana. Similar macromorphological and anatomical characteristics are shared by these new species: arachnoid basidiocarps; byssoid sterile margins; utriform basidia with a clamp connection at the base; the absence of rhizomorphs and cystidia; and slightly thick-walled, subglobose to globose basidiospores. Among these new species, the color of the hymenophoral surface, the size of the basidiospores, and some other features can be used for species delimitation. The new species and closely related species in the phylogenetic tree were discussed, and a key to the identified species of Tomentella from China was provided.

scholarly journals Five Fungal Pathogens Are Responsible for Bayberry Twig Blight and Fungicides Were Screened for Disease Control

2020 ◽  
Vol 8 (5) ◽  
pp. 689 ◽  
Author(s):  
Wenjun Li ◽  
Ming Hu ◽  
Yang Xue ◽  
Zhijun Li ◽  
Yanfei Zhang ◽  
...  
Keyword(s):  
Ribosomal Rna ◽  
Fungal Pathogens ◽  
Large Subunit ◽  
Elongation Factor ◽  
Morphological Characteristics ◽  
Its Region ◽  
Small Subunit ◽  
Field Application ◽  
Financial Loss ◽  
Twig Blight

Bayberry (Myrica rubra) is a commercial fruit in China. For the past seven years, twig blight disease has been attacking bayberry plantations in Shantou City, Guangdong Province, China, leading to destructive damage and financial loss. In this study, five fungal species associated with twig dieback and stem blight were identified based on morphological characteristics combined with multilocus sequence analysis (MLSA) on the internal transcribed spacer (ITS) region, partial sequences of β-tubulin (tub2), translation elongation factor 1-α (tef1-α), large subunit ribosomal RNA (LSU) and small subunit ribosomal RNA (SSU) genes, which are Epicoccum sorghinum, Neofusicoccum parvum, Lasiodiplodia theobromae, Nigrospora oryzae and a Pestalotiopsis new species P. myricae. P. myricae is the chief pathogen in fields, based on its high isolation rate and fast disease progression after inoculation. To our knowledge, this is the first study reporting the above five fungi as the pathogens responsible for bayberry twig blight. Indoor screening of fungicides indicates that Prochloraz (copper salt) is the most promising fungicide for field application, followed by Pyraclostrobin, 15% Difenoconazole + 15% Propiconazole, Difenoconazole and Myclobutanil. Additionally, Bacillus velezensis strain 3–10 and zeamines from Dickeya zeae strain EC1 could be used as potential ecofriendly alternatives to control the disease.

scholarly journals Cibaomyces, a new genus of Physalacriaceae from East Asia

Phytotaxa ◽  
2014 ◽  
Vol 162 (4) ◽  
pp. 198 ◽  
Author(s):  
Hao Yan-Jia ◽  
Qin Jiao ◽  
Yang Zhu-Liang
Keyword(s):  
Ribosomal Rna ◽  
New Genus ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Molecular Evidence ◽  
Internal Transcribed Spacer Region ◽  
Line Drawings ◽  
Phylogenetic Placement ◽  
Molecular Phylogenetic ◽  
Thin Walled

A new genus in Physalacriaceae, Cibaomyces, typified by C. glutinis, is described using morphological and molecular evidence. Cibaomyces is morphologically characterized by the combination of the following characters: basidioma small to medium-sized, collybioid to tricholomatoid; pileus viscid; hymenophore sinuate to subdecurrent, relatively distant, with brown lamellar edge; stipe sticky and densely covered with felted squamules; basidiospores thin-walled, ornamented with finger-like projections; cystidia nearly cylindrical, thin-walled, often heavily incrusted. Molecular phylogenetic analyses using DNA nucleotide sequences of the internal transcribed spacer region and the large subunit nuclear ribosomal RNA loci indicated that Cibaomyces was related to Gloiocephala, Laccariopsis and Rhizomarasmius. A description, line drawings, phylogenetic placement and comparison with allied taxa are presented. 

scholarly journals Four new species in the Tremella fibulifera complex (Tremellales, Basidiomycota)

MycoKeys ◽  
2021 ◽  
Vol 82 ◽  
pp. 33-56
Author(s):  
Long-Fei Fan ◽  
Renato Lúcio Mendes Alvarenga ◽  
Tatiana Baptista Gibertoni ◽  
Fang Wu ◽  
Yu-Cheng Dai
Keyword(s):  
New Species ◽  
Rna Polymerase Ii ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Elongation Factor ◽  
Morphological Characteristics ◽  
Molecular Data ◽  
Small Subunit ◽  
Molecular Evidence ◽  
Rrna Gene

Samples of species close to Tremella fibulifera from China and Brazil are studied, and T. fibulifera is confirmed as a species complex including nine species. Five known species (T. cheejenii, T. fibulifera s.s., T. “neofibulifera”, T. lloydiae-candidae and T. olens) and four new species (T. australe, T. guangxiensis, T. latispora and T. subfibulifera) in the complex are recognized based on morphological characteristics, molecular evidence, and geographic distribution. Sequences of eight species of the complex were included in the phylogenetic analyses because T. olens lacks molecular data. The phylogenetic analyses were performed by a combined sequence dataset of the internal transcribed spacer (ITS) and the partial nuclear large subunit rDNA (nLSU), and a combined sequence dataset of the ITS, partial nLSU, the small subunit mitochondrial rRNA gene (mtSSU), the translation elongation factor 1-α (TEF1), the largest and second largest subunits of RNA polymerase II (RPB1 and RPB2). The eight species formed eight independent lineages with robust support in phylogenies based on both datasets. Illustrated description of the six species including Tremella fibulifera s.s., T. “neofibulifera” and four new species, and discussions with their related species, are provided. A table of the comparison of the important characteristics of nine species in the T. fibulifera complex and a key to the whitish species in Tremella s.s. are provided.

Dothidea kunmingensis, a novel asexual species of Dothideaceae on Jasminum nudiflorum (winter jasmine) from Southwestern China

Phytotaxa ◽  
2021 ◽  
Vol 529 (1) ◽  
pp. 43-56
Author(s):  
YING GAO ◽  
JUTAMART MONKAI ◽  
ELENI GENTEKAKI ◽  
GUANG-CONG REN ◽  
DHANUSHKA N. WANASINGHE ◽  
...  
Keyword(s):  
New Species ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Elongation Factor ◽  
Morphological Characteristics ◽  
Small Subunit ◽  
Morphological Characterization ◽  
Small Subunit Rrna ◽  
Sister Relationship ◽  
Large Subunit Rrna

During a survey of saprobic microfungi in Southwest China, a coelomycetous fungus was found on dead twigs of Jasminum nudiflorum in Kunming, Yunnan Province. Based on a detailed morphological characterization coupled with multi-locus phylogenetic analyses, the fungus was identified as a new species in the genus Dothidea. Phylogenetic analyses using a combined matrix consisting of internal transcribed spacer (ITS), large subunit rRNA (LSU), small subunit rRNA (SSU), beta tubulin (tub2) and translation elongation factor-1 alpha (tef1-α) confirmed its placement in Dothideaceae and revealed a sister relationship to Dothidea eucalypti. The new species is characterized by pycnidial conidiomata, ampulliform or doliiform conidiogenous cells as well as aseptate, subglobose to ovoid, hyaline to pale-brown conidia. Comprehensive descriptions and illustrations are provided. Morphological characteristics of asexual morph taxa in Dothideaceae are also summarized and discussed.

Morphological characteristics and phylogenetic analyses revealed a new Calocybe (Lyophyllaceae, Basidiomycota) species from northeast China

Phytotaxa ◽  
2021 ◽  
Vol 490 (2) ◽  
pp. 203-210
Author(s):  
JIZE XU ◽  
XIAODONG YU ◽  
CHUNLAN ZHANG ◽  
YU LI
Keyword(s):  
New Species ◽  
Internal Transcribed Spacer ◽  
Northeast China ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Morphological Characteristics ◽  
Nuclear Ribosomal Dna ◽  
Full Description ◽  
Molecular Phylogenetic ◽  
A New Species

A new species, Calocybe decurrens, is illustrated and described in detail based on morphological characteristics and phylogenetic analyses. Calocybe decurrens is mainly characterized by its decurrent gills and by its stipe that discolors upon maturation. Molecular phylogenetic analyses were based on the internal transcribed spacer (ITS1-5.8S-ITS2) and the large subunit of the nuclear ribosomal DNA (nrLSU) sequences. The results indicated that its affiliation is in genus Calocybe, where it occupies an isolated position. A full description, color images, illustrations and a phylogenetic tree to show the placement of the new species are provided.

Sign in / Sign up

Export Citation Format

Share Document