Species identification and phylogenetic analysis of Leishmania isolated from patients, vectors and hares in the Xinjiang Autonomous Region, The People’s Republic of China

Background Visceral leishmaniasis (VL) has been declared as one of the six major tropical diseases by the World Health Organization. This disease has been successfully controlled in China, except for some areas in the western region, such as the Xinjiang Autonomous Region, where both anthroponotic VL (AVL) and desert type zoonotic VL (DT-ZVL) remain endemic with sporadic epidemics. Methodology/Principal findings Here, an eleven-year survey (2004–2014) of Leishmania species, encompassing both VL types isolated from patients, sand-fly vectors and Tarim hares (Lepus yarkandensis) from the Xinjiang Autonomous Region was conducted, with a special emphasis on the hares as a potential reservoir animal for DT-ZVL. Key diagnostic genes, ITS1, hsp70 and nagt (encoding N-acetylglucosamine-1-phosphate transferase) were used for phylogenetic analyses, placing all Xinjiang isolates into one clade of the L. donovani complex. Unexpectedly, AVL isolates were found to be closely related to L. infantum, while DT-ZVL isolates were closer to L. donovani. Unrooted parsimony networks of haplotypes for these isolates also revealed their relationship. Conclusions/Significance The above analyses of the DT-ZVL isolates suggested their geographic isolation and independent evolution. The sequence identity of isolates from patients, vectors and the Tarim hares in a single DT-ZVL site provides strong evidence in support of this species as an animal reservoir.

A new species of genus Prosopigastra A. Costa (Hymenoptera: Crabronidae: Crabroninae) from China, with a key to the Chinese species

The Chinese species of Prosopigastra A. Costa (Crabronidae: Crabroninae) are reviewed, including one species previously recorded from China and two species newly recorded from there. A new species, Prosopigastra simpliclypeata from Xinjiang Autonomous Region, China is described and illustrated. A key to the Chinese species of Prosopigastra is provided.

The Sogdians and Their Religions in Turfan: Evidence in the Catalogue of the Middle Iranian Fragments in Sogdian Script of the Berlin Turfan Collection

We are able to verify the variety of the religions of the Sogdians by the text fragments found in the Turfan oasis (East Turkistan, today’s Xinjiang Autonomous Region of China). They are housed in several libraries and museums in Europe, Japan, and China. The Berlin Turfan collection contains a large part of them. The catalogue of the Sogdian text fragments in the indigenous Sogdian script of that collection was completed in 2018. The fragments represent parts of the literature of Christian, Manichaean and Buddhist communities in Turfan from the eighth to eleventh century CE. The best represented religion in the homeland of the Sogdians is a type of the Zoroastrian religion, as evidenced by archaeological findings and wall paintings. However, there are only very few texts found in Turfan and other locations in Central Asia which could be interpreted as Zoroastrian. The discussion about the religious affiliation of those texts is going on. The religious background of some other text fragments from Turfan is difficult to identify as well. Two of these examples will be published here. A remarkable feature of the religious communities in Turfan is the multilingual character of their literature, reflecting the development and path of the believers and the multi-ethnical structure of the community.

New Species of Tomentella (Thelephorales, Basidiomycota) from Temperate Continental Mountain Climate of China (Xinjiang Region)

Species in the genus Tomentella are distributed throughout the temperate and tropical regions worldwide, but few studies associated with the taxonomy and phylogeny of this genus had been reported from Northwest China. In this paper, molecular phylogenetic analyses of the nuclear ribosomal ITS (internal transcribed spacer: ITS1-5.8S-ITS2) and LSU (large subunit: 28S) sequences combined with morphological characteristics identified three new species from Xinjiang Autonomous Region in Northwest China, which were named T. aurantispora, T. kanasensis, and T. schrenkiana. Similar macromorphological and anatomical characteristics are shared by these new species: arachnoid basidiocarps; byssoid sterile margins; utriform basidia with a clamp connection at the base; the absence of rhizomorphs and cystidia; and slightly thick-walled, subglobose to globose basidiospores. Among these new species, the color of the hymenophoral surface, the size of the basidiospores, and some other features can be used for species delimitation. The new species and closely related species in the phylogenetic tree were discussed, and a key to the identified species of Tomentella from China was provided.

Capronia pilosella. [Descriptions of Fungi and Bacteria].

A description is provided for Capronia pilosella, found on unidentified, very rotten wood. Some information on its morphological characteristics, associated organisms and substrata, dispersal and transmission, habitats and economic impacts is given, along with details of its geographical distribution (Asia (China, Xinjiang Autonomous Region, Taiwan), Australasia (Australia, Australian Capital Territory, New South Wales, Northern Territory, South Australia, Tasmania, Victoria, New Zealand), Europe (Austria, Belgium, Croatia, Czech Republic, Denmark, Finland, France, Germany, Ireland, Netherlands, Norway, Spain, Sweden, Switzerland, Ukraine, UK), North America (Canada, Ontario, Prince Edward Island, USA, Idaho, Indiana, Iowa, Missouri, New Hampshire, New Jersey, New York), Pacific Ocean (Fiji), South America (Argentina, Brazil, Amazonas)).

Investigating the sensitivity of Venturia inaequalis isolates to difenoconazole and pyraclostrobin in apple orchards in China

The resistance level of 90 single lesion conidial isolates of Venturia inaequalis collected from multiple commercial orchards in Shaanxi and Gansu Provinces and Xinjiang Autonomous Region of China to the demethylation inhibitor (DMI) fungicide difenoconazole and quinone outside inhibitor (QoI) fungicide pyraclostrobin was examined. The EC50 values of the 90 isolates to difenoconazole and pyraclostrobin ranged from 0.143 to 6.735 μg/mL and 0.084 to 2.026 μg/mL, respectively. Among the isolates, 19 had resistance, 66 had reduced sensitivity, and five had sensitivity to difenoconazole; eight had resistance, 81 had reduced sensitivity, and one had sensitivity to pyraclostrobin. Although a weak correlation between difenoconazole and pyraclostrobin was detected, four isolates were identified as resistant to difenoconazole and pyraclostrobin. However, isolates with practical resistance were not found widely in our study and were only sporadic in a few places, indicating that at present, difenoconazole and pyraclostrobin are still safe for disease management in the apple-growing areas of Shaanxi, Gansu and Xinjiang. However, the risk of fungicide resistance should be managed with caution, and yearly monitoring of resistance development is necessary.

Case study of the beneficiary group distribution of curative care expenditure based on SHA 2011 in Xinjiang autonomous region, China

BackgroundThe System of Health Accounts 2011 (SHA 2011) assists in health policy analysis and health expenditure comparison at the international level. Based on SHA 2011, this study analysed the distribution of beneficiary groups of curative care expenditure (CCE) in Xinjiang, to present suggestions for developing health policies.MethodsA total of 160 health institutions were selected using the multistage stratified random sampling method. An analysis of the agewise CCE distribution, institutional flow, and disease distribution was then performed based on the SHA 2011 accounting framework.ResultsIn 2016, the CCE in Xinjiang was ¥50.05 billion, accounting for 70.18% of current health expenditure and 6.66% of the gross domestic product. The per capita CCE was ¥2366.56. The CCE was distributed differently across age groups, with the highest spending on people over the age of 65 years. The CCE was highest for diseases of the circulatory, respiratory and digestive systems. Most of the expenditure was incurred in hospitals and, to a lesser extent, in primary healthcare institutions. Family health expenditure, especially on children aged 14 years and below, accounted for a relatively high proportion of the CCE.ConclusionSHA 2011 was used to capture data, which was then analysed according to the newly added beneficiary dimension. The findings revealed that the use of medical resources is low, the scale of primary medical institutions needs to be significantly expanded and there is a need to optimise the CCE financing scheme. Therefore, the health policymaking department should optimise the relevant policies and improve the efficiency of health services.

High endemicity of alveolar echinococcosis in Yili Prefecture, Xinjiang Autonomous Region, the People’s Republic of China: Infection status in different ethnic communities and in small mammals

Alveolar echinococcosis (AE) is a life-threatening disease in humans caused by the larval stage of Echinococcus multilocularis. The tapeworm is transmitted between small mammals and dogs/foxes in the Northern Hemisphere. In this study 286 AE cases were reported from eight counties and one city in Yili Prefecture, Xinjiang Autonomous Region, the People’s Republic of China from 1989 to 2015 with an annual incidence (AI) of 0.41/100,000. Among the patients, 73.08% were diagnosed in the last 11 years. Four counties in the high mountainous areas showed higher AI (0.51–1.22 cases/100,000 residents) than the four counties in low level areas (0.19–0.29/100,000 residents). The AI of AE in Mongolian (2.06/100,000 residents) and Kazak (0.93/100,000 residents) ethnic groups was higher than the incidence in other ethnic groups indicating sheep-farming is a risk for infection given this activity is mainly practiced by these two groups in the prefecture. A total of 1411 small mammals were captured with 9.14% infected with E. multilocularis metacestodes. Microtus obscurus was the dominant species in the mountain pasture areas with 15.01% of the voles infected, whereas Mus musculus and Apodemus sylvaticus were the dominant small mammals in the low altitude areas. Only 0.40% of A. sylvaticus were infected with E. multilocularis. PCR amplification and sequencing analysis of the mitochondrial cox1 gene showed that E. multilocularis DNA sequences from the small mammals were identical to isolates of local human AE cases. The overall results show that Yili Prefecture is a highly endemic area for AE and that the high-altitude pasture areas favorable for M. obscurus may play an important role in its transmission in this region.

Mechanistic Investigation of Typical Loess Landslide Disasters in Ili Basin, Xinjiang, China

In the period from 2010 to 2018, a total of 302 geological disasters occurred in the Xinjiang Autonomous Region, China, of which 136 occurred in the Ili Basin. Compared with those in other regions, the loess landslides in the Ili Basin are strongly influenced by the seasonal freeze–thaw effect. Taking the No. 2 Piliqinghe landslide as an example and based on the field geological investigation, it was found in the present study that the main triggering factors of this landslide were the snowmelt erosion of the slope toe and meltwater infiltration into the trailing edge of the slope. The mechanism of loess landslide instability was studied using numerical simulation. The results showed that (1) the Piliqinghe landslide disaster was formed through a process composed of the local sliding of the leading edge → the creep sliding and tension cracking of the slope surface → the overall sliding stage; (2) the infiltration of snowmelt was the direct cause of the landslide formation; (3) the fluvial erosion and softening caused the soil of the slope toe to slide. The results can be used as a reference for the analysis of the disaster mechanism and movement characteristics of similar loess landslides.