Morphology, anatomy and palynology study on Turkish endemic species Saponaria karapinarensis (Caryophyllaceae)

Phytotaxa ◽  
2018 ◽  
Vol 374 (1) ◽  
pp. 80
Author(s):  
DENİZ ULUKUŞ
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Endemic Species ◽  
Pollen Grains ◽  
Point Of View ◽  
Anatomical Features ◽  
Exine Ornamentation ◽  
Cell Layers ◽  
Taxonomic Point ◽  
Scanning Electron

Saponaria karapinarensis (Caryophyllaceae), an endemic species from Turkey, was studied from taxonomic point of view. Morphological, anatomical, and palynological characteristics of the species were examined. Morphology of stem, calyx, petals, and capsule are useful to distinguish the species. Anatomical features, such as the number of cortex layers and sclerenchymatic cell layers in stem, are of taxonomical importance. SEM Scanning Electron Microscopy (SEM) studies on the pollen grains have revealed that they are spheroideae and their exine ornamentation is granulatae, spinulate microechinatae- microperforatae. Seed micromorphological features were also given.

scholarly journals Pollen morphology of the Balkan-Carpathian endemic Campanula lingulata Waldst. & Kit. (Campanulaceae)

2016 ◽  
pp. 75-82
Author(s):  
Marina Macukanovic-Jocic ◽  
Snezana Jaric
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Light Microscopy ◽  
Endemic Species ◽  
Pollen Morphology ◽  
Average Length ◽  
Pollen Grains ◽  
Polar Axis ◽  
First Time ◽  
Scanning Electron

Palynomorphological characteristics of Campanula lingulata, the Balkan-Carpathian endemic species growing in Serbia, have been investigated using light microscopy and scanning electron microscopy for the first time, in order to provide some information helpful for a better understanding of the taxonomic position of this species within the genus, as well as to contribute to the pollen atlas of Serbian apiflora. The pollen grains are radially symmetrical, isopolar, 3-zonoporate and medium-sized monads oblate-sphaeroidal in shape. Mean of the polar axis (P) is 27.6?1.9 ?m, while the average length of the equatorial axis (E) is 28.8?1.6 ?m. The apertures are operculate. The sculpturing pattern of the exine is microre?ticulate-microechinatae. The exine surface is covered with evenly distributed supratectal spinules of variable length and sparse granules. The longest supratectal spinules are 0.64?0.05 ?m in length and the smallest sculptural elements are less than 0.2 ?m high. The microechinae density per sample area of 5 ?m x 5 ?m averages 17.4?2.4.

scholarly journals Distinguishing Oblacinska sour cherry clones (Prunus cerasus L.) by pollen morphology

Genetika ◽  
2020 ◽  
Vol 52 (1) ◽  
pp. 187-198 ◽  
Author(s):  
Dragan Nikolic ◽  
Dragan Milatovic ◽  
Aleksandar Radovic ◽  
Jugoslav Trajkovic
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Pollen Morphology ◽  
Pollen Grains ◽  
Sour Cherry ◽  
Prunus Cerasus ◽  
Morphological Parameters ◽  
Exine Ornamentation ◽  
Pollen Shape ◽  
Scanning Electron

Morphology and ultrastructure of pollen grains were studied in 13 Obla?inska sour cherry clones in three years using scanning electron microscopy (SEM). All studied clones had isopolar, radially symmetric and tricolpate pollen grains. Length and width of pollen grains varied in a range of 50.03-55.97 ?m and 25.16-28.37 ?m respectively. Pollen shape in all the studied clones was identified as prolate. The smallest colpus length was found in the clone 8 (43.73 ?m), and the highest in the clone 3 (49.16 ?m). The highest colpus and mesocolpium width had the clone 3 (1.73 ?m; 14.87 ?m) and the lowest had the clone 7 (1.46 ?m; 13.78 ?m). All studied clones had striate exine ornamentation. Number of ridges per 100 ?m2 of the exine surface was the highest in the clone 11 (16.7) and lowest in the clone 2 (14.1). Ridge and furrow width ranged from 0.46 to 0.61 ?m and from 0.45 to 0.59 ?m, respectively. The clones have been classified into three clusters based on all studied properties. Pollen grains examination by SEM indicated that several morphological parameters (pollen size and exine characteristics) can be used to distinguish Obla?inska sour cherry clones.

Morphological, cytological, palynological and molecular evidence on two new hybrids from Turkey: an example of homoploid hybridization in Origanum (Lamiaceae)

Phytotaxa ◽  
2018 ◽  
Vol 371 (3) ◽  
pp. 145 ◽  
Author(s):  
TUNCAY DİRMENCİ ◽  
TANER ÖZCAN ◽  
TÜRKER YAZICI ◽  
TURAN ARABACI ◽  
ESRA MARTİN
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Somatic Chromosome ◽  
Pollen Grains ◽  
Molecular Evidence ◽  
Hybrid Swarms ◽  
Pollen Characters ◽  
Exine Ornamentation ◽  
Nuclear Its ◽  
Scanning Electron

Two new hybrids of Origanum, O. ×malyeri and O. ×sevcaniae, are described and illustrated in detail. The general morphology, pollen and chromosome features, and nuclear (ITS) and chloroplast (rpl32) genomes of collected specimens were studied. Origanum ×malyeri is a hybrid between O. boissieri and O. vulgare subsp. hirtum and O. ×sevcaniae is another hybrid between O. vulgare subsp. hirtum and O. vogelii. These two new hybrids have some morphological intermediate characters between their parents. The pollen morphology of O. ×sevcaniae and its parents were studied and documented in detail using light microscopy (LM) and scanning electron microscopy (SEM). O. ×sevcaniae pollen is small to medium (P=18.49–21.03 µm, E=22.20–26.63 µm), suboblate (73%) to oblate (22%), the range of P/E ratio is from 0.73 to 0.85. Pollen grains are hexacolpate with granular membranes. Pollen grains were identified as bireticulate exine ornamentation with scanning electron microscopy examinations. The somatic chromosome number of O. boissieri, O. vulgare subsp. hirtum, O. vogelii, and O. ×sevcaniae were counted as 2n = 30. Pollen characters and somatic chromosome numbers of O. ×malyeri could not be examined because it has only female flowers and has not any nutlets. Also, according to molecular results, O. ×malyeri and O. ×sevcaniae have some polymorphic nucleotide regions in their nuclear ITS genomes and which means they have two different DNA dataset belonging to their parents. Also, some individuals of O. ×sevcaniae parents have single nucleotide polymorphism, too. These individuals live together with their hybrids and hybridization is probably a continuing process in that hybrid swarms. These present results support previous reports for the speciation of Origanum members via hybridization.

scholarly journals Anatomy Differs for Aborting and Nonaborting Pistillate Flowers in Pecan

1994 ◽  
Vol 119 (5) ◽  
pp. 949-955 ◽  
Author(s):  
I.E. Yates ◽  
Darrell Sparks
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Embryo Sac ◽  
Central Nucleus ◽  
Tissue Necrosis ◽  
Carya Illinoensis ◽  
Anatomical Features ◽  
Cell Layers ◽  
Scanning Electron

Comparative anatomical features of nonaborting (normal) and aborting pistillate flowers were examined with light and scanning electron microscopy during the first of four physiological drops characteristic of pecan [Carya illinoensis (Wangenh.) C. Koch]. Flowers sampled over a 3-year period from a protandrous and a protogynous cultivar (Desirable and Wichita, respectively) did not have any tissue necrosis. Diameter, length, and weight of aborting intact flowers were significantly less at 65%, 55%, and 30%, respectively, in aborting than nonaborting intact flowers. A common anatomical deviation in aborting flowers was that the integument was less extended over the nucellus of the ovule than in nonaborting flowers. The number of parenchymal nucellus cell layers lateral to the embryo sac often was less in aborting than nonaborting flowers. Embryo sacs were inflated in nonaborting flowers, but appeared deflated in aborting flowers. Both sacs had a conspicuous central nucleus, egg, and synergid, with a second synergid evident in the embryo sacs of some nonaborting flowers. Thus, aborting pecan flowers had incompletely developed ovules with no evidence of necrosis.

Inheritance of exine ornamentation and pollen shape in the interspecific tetraploid hybrids of Gloriosa

1986 ◽  
Vol 64 (12) ◽  
pp. 3134-3140 ◽  
Author(s):  
C. Ravi kumar ◽  
P. K. K. Nair
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Light Microscopy ◽  
Maternal Inheritance ◽  
Parental Species ◽  
Pollen Grains ◽  
The Other ◽  
Exine Ornamentation ◽  
Pollen Shape ◽  
Scanning Electron

Scanning electron microscopy and light microscopy of the pollen of three tetraploid (2n = 44) species, Gloriosa virescens Lindle, G. carsonii Baker, and G. richmondensis Hort., and three of their hybrids showed that the pollen in Gloriosa L. fundamentally consists of three pollen shape types, spheroidal, ellipsoidal, and spindle shaped, having monocolpate aperture and a striate exine ornamentation pattern. The three parental species can be differentiated from each other on the basis of pollen shape and their characteristic striate exine ornamentation. However, in all three hybrids the composition of pollen shape suggests maternal inheritance of the character. Furthermore, in each of the three hybrids, new exine ornamentation features have appeared which differ from those of the other hybrids and from their respective parents. All the pollen grains in each of the hybrids are uniform with the same characteristic exine ornamentation. They show no segregation of exine features into the parental categories, which would be expected if exine ornamentation was under sporophytic control. The potential of exine features as genetic markers in hybrids is discussed.

Pollen grain micromorphology in some genera of Ornithogaleae (Asparagaceae, Scilloideae) and its relative significance

Phytotaxa ◽  
2020 ◽  
Vol 458 (4) ◽  
pp. 242-256
Author(s):  
MEHDI HEIDARIAN ◽  
SEYED MOHAMMAD MAHDI HAMDI ◽  
MOHAMMAD MEHDI DEHSHIRI ◽  
TAHER NEJADSATTARI ◽  
SEYED MOHAMMAD MASOUMI
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Pollen Grains ◽  
Pollen Grain ◽  
Relative Significance ◽  
Phylogenetic Studies ◽  
Transmission Electron ◽  
Exine Ornamentation ◽  
Tem Transmission Electron Microscopy ◽  
Scanning Electron

In this research, the pollen micromorphology of nine species belonging to Honorius, Loncomelos, Ornithogalum and Stellarioides are observed by LM (Light Microscopy), SEM (Scanning Electron Microscopy) and two species by TEM (Transmission Electron Microscopy). Lately, the phylogenetic studies have determined nineteen monophyletic genera in subfamily Ornithogaloideae (=tribe Ornithogaleae). Our results show that the pollen grains characters such as shape, size, exine ornamentation on equatorial surface, sulcus margin exine ornamentation, sulcus membrane ornamentation and lumina number on exine surface are varied among all studied genera. The dendrogram of palynological characters analyzed on LM and SEM observation by MVSP (Multi Variate Statistical Package) software partly supports the monophyletic genera. According to the obtained palynological dendrogram, Loncomelos arcuatum and Ornithogalum sintenisii are placed in one subtype. Based on the sporoderm structure (exine thickness and the endexine type), L. arcuatum differs from O. sintenisii. Finally, the palynological characters can be useful for clarifying the taxonomic relationship in this subfamily.

scholarly journals Pollen morphology of Iranian Dracocephalum L. (Lamiaceae) and its taxonomic significance

2015 ◽  
Vol 22 (2) ◽  
pp. 99-110 ◽  
Author(s):  
Maryam Naderifar ◽  
Ali Sonboli ◽  
Abbas Gholipour
Keyword(s):  
Electron Microscopy ◽  
Pollen Morphology ◽  
Pollen Grains ◽  
Polar View ◽  
Taxonomic Significance ◽  
Infrageneric Classification ◽  
Exine Ornamentation ◽  
Plant Taxon ◽  
Scanning Electron

Pollen morphology of 11 Iranian Dracocephalum L. species was investigated using light and scanning electron microscopy to evaluate their taxonomic significance for the infrageneric classification of the genus. Pollen grains of all examined taxa were isopolar, hexacolpate, circular in polar view and spheroidal to prolate in equatorial view (P/E = 1.0?2.0). The smallest pollen grains were observed in D. aucheri (P = 29.7 ?m, E = 22.6 ?m), while the largest pollen was found in D. lindbergii (P = 45.1 ?m, E = 33.7 ?m). The highest and lowest apocolpium index (AI) were measured in D. aucheri (AI = 0.27) and D. surmandinum (AI = 0.08), respectively. Colpus membrane was egranulate in all examined species except for D. multicaule and D. ghahremanii. The main exine ornamentation type was characterized as bireticulate including five different subtypes. The results revealed that the exine ornamentation is a diagnostic character useful for the classification of Dracocephalum.Bangladesh J. Plant Taxon. 22(2): 99-110, 2015 (December)

scholarly journals Pollen morphology of the genus Eremanthus Less. (Vernonieae, Asteraceae)

2012 ◽  
Vol 26 (1) ◽  
pp. 46-57 ◽  
Author(s):  
BenoÎt Loeuille ◽  
Raquel Maria Batista Souza-Souza ◽  
Vanessa Holanda Righetti Abreu ◽  
Cláudia Barbieri Ferreira Mendonça ◽  
Vania Gonçalves-Esteves
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Pollen Morphology ◽  
Additional Data ◽  
Phylogenetic Reconstruction ◽  
Pollen Grains ◽  
Prolate Spheroidal ◽  
Oblate Spheroidal ◽  
Quantitative Characters ◽  
Scanning Electron

In order to evaluate the significance of the pollen morphology for generic and infrageneric taxonomy of the genus Eremanthus (Vernonieae, Asteraceae), and to provide additional data for its phylogenetic reconstruction, the pollen of 20 of the 23 species of the genus was examined using light and scanning electron microscopy. Acetolysed pollen grains were measured, described, and illustrated using light microscopy, while non-acetolysed pollen grains were analyzed using scanning electron microscopy. Pollen grains of these species are isopolar, oblate-spheroidal in most of the species, more rarely prolate spheroidal or suboblate, subtriangular amb, tricolporate and subechinolophate. The variation among quantitative characters does not correlate with the macromorphological subdivision of the genus or with the generic or specific limits.

scholarly journals Pollen morphology of Ukrainian species of the genus Pedicularis L. (Orobanchaceae Vent.)

2012 ◽  
Vol 24 (1) ◽  
pp. 5-13 ◽  
Author(s):  
Olena M. Peregrym ◽  
Zoya M. Tsymbalyuk ◽  
Sergei L. Mosyakin
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Pollen Morphology ◽  
Pollen Grains ◽  
Taxonomic Revision ◽  
Palynological Data ◽  
First Time ◽  
Scanning Electron

Abstract Pollen grains of 10 species of Pedicularis occurring in Ukraine were investigated using light and scanning electron microscopy. Palynological data on 6 species are reported for the first time. General pollen morphology of the genus Pedicularis is presented. Three types of apertures (2-syncolpate, 3-colpate, and 3-syncolpate) and seven subtypes of sculpture (3-syncolpate pilate, 3-syncolpate microscabrate-tubeculate, 2-syncolpate microscabrate, 2-syncolpate microscabrate-tubeculate, 2-syncolpate microscabrate-tubeculate-perforate, 2-syncolpate microfoveolate, and 2-syncolpate microscabrate-perforate) are indentified. The subgenus Pedicularis is heterogeneous in its types of apertures and sculpture of the surface, which indicates the need of further taxonomic revision of the group.

scholarly journals ELECTRON MICROSCOPIC STUDIES OF SOME ANTICANCER PLANTS POLLEN GRAINS

2021 ◽  
Vol 9 (10) ◽  
pp. 1386-1393
Author(s):  
Jayshree Sandesh Thaware ◽  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Pollen Morphology ◽  
Pollen Grains ◽  
Morphological Characters ◽  
Electron Microscopic ◽  
Plant Parts ◽  
Morphological Studies ◽  
Datura Metel ◽  
Scanning Electron

Pollen is appropriately referred by some as Golden dust extremely valuable on account of their tremendous applications in science, industries and public health. No other plant part even though extremely tiny in size is packed with so much information and power. Similar to other plant parts, pollen characters are so varied that the classification system of plants can be built up entirely on the basis of pollen morphology.Palynology is the distinct branch of biology that deals with the dispersed microscopic tiny living and fossil entities including pollen grains, spores, algal and fungal fragments and others. An important aspect of Palynology is the Pollen morphology. The importance of Palynology in taxonomic and phylogenetic consideration of plants is well known. The changes occurring through hybridization and years of cultivation are reflected in pollen morphology. The scope and interest in the study of pollen morphology have widened with the advent of Scanning Electron Microscopy (SEM) and with regards to unipalynous taxa particularly the understanding of finer morphology is of fundamental importance. SEM gives a correct understanding of exine surface as the electron photographs of the surface replica of the exine provides the exact picture of the ornamentation pattern. The variation in the pollen morphological characters helps in the classification of plant taxa and their assessment of their phylogenetic relationship. In the present investigation, the pollen morphological studies were carried out of some ethnomedicinal plants like Catharanthus roseus, Allamanda cathartica, Datura metel, Brassica juncea, Raphanus sativus and Cleome viscosa pollen grains by Scanning electron microscopy. All that they possess anticancer characteristics in common.

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