scholarly journals ELECTRON MICROSCOPIC STUDIES OF SOME ANTICANCER PLANTS POLLEN GRAINS

2021 ◽  
Vol 9 (10) ◽  
pp. 1386-1393
Author(s):  
Jayshree Sandesh Thaware ◽  

Pollen is appropriately referred by some as Golden dust extremely valuable on account of their tremendous applications in science, industries and public health. No other plant part even though extremely tiny in size is packed with so much information and power. Similar to other plant parts, pollen characters are so varied that the classification system of plants can be built up entirely on the basis of pollen morphology.Palynology is the distinct branch of biology that deals with the dispersed microscopic tiny living and fossil entities including pollen grains, spores, algal and fungal fragments and others. An important aspect of Palynology is the Pollen morphology. The importance of Palynology in taxonomic and phylogenetic consideration of plants is well known. The changes occurring through hybridization and years of cultivation are reflected in pollen morphology. The scope and interest in the study of pollen morphology have widened with the advent of Scanning Electron Microscopy (SEM) and with regards to unipalynous taxa particularly the understanding of finer morphology is of fundamental importance. SEM gives a correct understanding of exine surface as the electron photographs of the surface replica of the exine provides the exact picture of the ornamentation pattern. The variation in the pollen morphological characters helps in the classification of plant taxa and their assessment of their phylogenetic relationship. In the present investigation, the pollen morphological studies were carried out of some ethnomedicinal plants like Catharanthus roseus, Allamanda cathartica, Datura metel, Brassica juncea, Raphanus sativus and Cleome viscosa pollen grains by Scanning electron microscopy. All that they possess anticancer characteristics in common.

1986 ◽  
Vol 64 (12) ◽  
pp. 3075-3078 ◽  
Author(s):  
N. Sahashi ◽  
J. Ueno

Morphological studies on pollen grains of Ginkgo biloba L. and Cycas revoluta Thunb. were carried out by scanning electron microscopy. The pollen grains of both species are generally oblong with 1-sulcate apertures which are shrunken as a result of dryness. However, the swollen grains show an almost spherical form with a large and rounded germinal aperture. This aperture may not correspond to any aperture type so far known, although the term "anaporate" can be fitted to the swollen pollen grains. Auricular projections, which may be derived from protrusions of the ectosexine, can be seen sometimes on the surface of the pollen grains. These projections remind us of degraded versions of the bladders that may have been present on the pollen grains of the fossil ancestor. The inner side of the exine, which can be seen in thin sections obtained with the freezing microtome, is ornamented with reticulumlike sculptures. These endosculptures may be the first reported among gymnosperm pollen grains.


2012 ◽  
Vol 26 (2) ◽  
pp. 444-455 ◽  
Author(s):  
Cristiano Eduardo Amaral Silveira Júnior ◽  
Marileide Dias Saba ◽  
Jomar Gomes Jardim

The palynology of the following 16 species of Rubiaceae, from Brejinho das Amestistas, was investigated: Coccocypselum hirsutum Bartl. ex DC., Cordiera rigida Kuntze, Coutarea hexandra K.Schum., Declieuxia fruticosa Kuntze, Diodella apiculata (Willd. ex Roem. & Schult.) Delprete, D. radula (Willd. ex Roem. & Schult.) Delprete, D. teres Small., Emmeorhiza umbellata K.Schum., Leptoscela ruellioides Hook. f., Mitracarpus baturitensis Sucre., Mitracarpus villosus Cham. & Schltdl., Palicourea rigida Kunth, Psyllocarpus asparagoides Mart., Richardia grandiflora Steud., Staelia aurea K. Schum., and Staelia galioides DC. The pollen grains were acetolysed to and their morphological characters were analyzed using light and scanning electron microscopy. They varied in size from small to large; were suboblate to subprolate; inaperturate (P. rigida), colpate and colporate in the remaining species, with an aperture number that varied from three to several. The exines were microreticulate in most species, reticulate (C. hirsutum, C. rigida and P. rigida), bireticulate (D. fruticosa), microechinate-perforated (C. hexandra), echinate-granulate (R. grandiflora), echinate-granulate-perforate (D. apiculata and D. teres), and psilate (P. asparagoides). Based on the results, palynological data can be used to distinguish these species.


2012 ◽  
Vol 26 (1) ◽  
pp. 46-57 ◽  
Author(s):  
BenoÎt Loeuille ◽  
Raquel Maria Batista Souza-Souza ◽  
Vanessa Holanda Righetti Abreu ◽  
Cláudia Barbieri Ferreira Mendonça ◽  
Vania Gonçalves-Esteves

In order to evaluate the significance of the pollen morphology for generic and infrageneric taxonomy of the genus Eremanthus (Vernonieae, Asteraceae), and to provide additional data for its phylogenetic reconstruction, the pollen of 20 of the 23 species of the genus was examined using light and scanning electron microscopy. Acetolysed pollen grains were measured, described, and illustrated using light microscopy, while non-acetolysed pollen grains were analyzed using scanning electron microscopy. Pollen grains of these species are isopolar, oblate-spheroidal in most of the species, more rarely prolate spheroidal or suboblate, subtriangular amb, tricolporate and subechinolophate. The variation among quantitative characters does not correlate with the macromorphological subdivision of the genus or with the generic or specific limits.


2012 ◽  
Vol 24 (1) ◽  
pp. 5-13 ◽  
Author(s):  
Olena M. Peregrym ◽  
Zoya M. Tsymbalyuk ◽  
Sergei L. Mosyakin

Abstract Pollen grains of 10 species of Pedicularis occurring in Ukraine were investigated using light and scanning electron microscopy. Palynological data on 6 species are reported for the first time. General pollen morphology of the genus Pedicularis is presented. Three types of apertures (2-syncolpate, 3-colpate, and 3-syncolpate) and seven subtypes of sculpture (3-syncolpate pilate, 3-syncolpate microscabrate-tubeculate, 2-syncolpate microscabrate, 2-syncolpate microscabrate-tubeculate, 2-syncolpate microscabrate-tubeculate-perforate, 2-syncolpate microfoveolate, and 2-syncolpate microscabrate-perforate) are indentified. The subgenus Pedicularis is heterogeneous in its types of apertures and sculpture of the surface, which indicates the need of further taxonomic revision of the group.


Author(s):  
Marina Macukanovic-Jocic ◽  
Snezana Jaric

Palynomorphological characteristics of Campanula lingulata, the Balkan-Carpathian endemic species growing in Serbia, have been investigated using light microscopy and scanning electron microscopy for the first time, in order to provide some information helpful for a better understanding of the taxonomic position of this species within the genus, as well as to contribute to the pollen atlas of Serbian apiflora. The pollen grains are radially symmetrical, isopolar, 3-zonoporate and medium-sized monads oblate-sphaeroidal in shape. Mean of the polar axis (P) is 27.6?1.9 ?m, while the average length of the equatorial axis (E) is 28.8?1.6 ?m. The apertures are operculate. The sculpturing pattern of the exine is microre?ticulate-microechinatae. The exine surface is covered with evenly distributed supratectal spinules of variable length and sparse granules. The longest supratectal spinules are 0.64?0.05 ?m in length and the smallest sculptural elements are less than 0.2 ?m high. The microechinae density per sample area of 5 ?m x 5 ?m averages 17.4?2.4.


Phytotaxa ◽  
2015 ◽  
Vol 207 (1) ◽  
pp. 135 ◽  
Author(s):  
Giovanni Raul Bogota ◽  
Carina Hoorn ◽  
Wim Star ◽  
Rob Langelaan ◽  
Hannah Banks ◽  
...  

Sabinaria magnifica is so far the only known species in the recently discovered tropical palm genus Sabinaria (Arecaceae). Here we present a complete description of the pollen morphology of this palm species based on light microscopy (LM), scanning electron microscopy (SEM) and transmission electron microscopy (TEM). We also made SEM-based comparisons of Sabinaria with other genera within the tribe Cryosophileae. Pollen grains of Sabinaria magnifica resemble the other genera in the heteropolar, slightly asymmetric monads, and the monosulcate and tectate exine with perforate surface. Nevertheless, there are some clear differences with Thrinax, Chelyocarpus and Cryosophila in terms of aperture and exine. S. magnifica differs from its closest relative, Itaya amicorum, in the exine structure. This study shows that a combination of microscope techniques is essential for the identification of different genera within the Cryosophileae and may also be a necessary when working with other palynologically less distinct palm genera. 


Phytotaxa ◽  
2018 ◽  
Vol 361 (2) ◽  
pp. 168 ◽  
Author(s):  
MUTLU GÜLTEPE ◽  
SERDAR MAKBUL ◽  
SEDA OKUR ◽  
KAMİL COŞKUNÇELEBİ

The pollen morphology of 25 Tragopogon L. taxa (including four subspecies and four varieties) distributed in Turkey was studied under light and scanning electron microscopy. It was observed that pollen grains of the examined Tragopogon taxa are suboblate and oblate-spheroidal in shape, and 3-zonocolpororate with fifteen lacunae. The lenghts of the polar axes and equatorial axes range from 31.57 to 40.35 µm and 35.32 to 44.65 µm, respectively. Numerical analyses show that the length of equatorial axis and the pore length are the most valuable characters among nineteen palynological traits for separating the examined taxa. The general palynological characteristics of the examined taxa allow some taxonomical evaluations for the genus. However, the results of cluster and principal component analyses did not support the division of the genus at any subgeneric level; neither did the ligule colour, as reported in the literature.


Genetika ◽  
2020 ◽  
Vol 52 (1) ◽  
pp. 187-198 ◽  
Author(s):  
Dragan Nikolic ◽  
Dragan Milatovic ◽  
Aleksandar Radovic ◽  
Jugoslav Trajkovic

Morphology and ultrastructure of pollen grains were studied in 13 Obla?inska sour cherry clones in three years using scanning electron microscopy (SEM). All studied clones had isopolar, radially symmetric and tricolpate pollen grains. Length and width of pollen grains varied in a range of 50.03-55.97 ?m and 25.16-28.37 ?m respectively. Pollen shape in all the studied clones was identified as prolate. The smallest colpus length was found in the clone 8 (43.73 ?m), and the highest in the clone 3 (49.16 ?m). The highest colpus and mesocolpium width had the clone 3 (1.73 ?m; 14.87 ?m) and the lowest had the clone 7 (1.46 ?m; 13.78 ?m). All studied clones had striate exine ornamentation. Number of ridges per 100 ?m2 of the exine surface was the highest in the clone 11 (16.7) and lowest in the clone 2 (14.1). Ridge and furrow width ranged from 0.46 to 0.61 ?m and from 0.45 to 0.59 ?m, respectively. The clones have been classified into three clusters based on all studied properties. Pollen grains examination by SEM indicated that several morphological parameters (pollen size and exine characteristics) can be used to distinguish Obla?inska sour cherry clones.


2019 ◽  
Vol 55 (1) ◽  
pp. 1-6 ◽  
Author(s):  
Zoya M. Tsymbalyuk ◽  
Sergei L. Mosyakin ◽  
Lyudmila M. Nitsenko

Abstract Pollen morphology of representatives of the genera Succisa and Succisella in the flora of Ukraine was studied using light and scanning electron microscopy. Pollen grains in both taxa were tricolpate, prolate, rarely oblate-spheroidal or spheroidal; large-sized. Their outline in polar view was subcircular, rarely trilobate, in equatorial view elliptical or rarely circular. Colpi short, of variable width, margins irregular with distinct, narrow margo, and blunt or acute ends. Exine sculpture was echinate-microechinate. The revealed characteristics of pollen grains are taxonomically significant at the generic and specific levels, and they can be used in pollen analysis. Palynomorphological data are consistent with the results of recent molecular phylogenetic studies. Data on pollen morphology also confirm taxonomic circumscription of Succiseae V. Mayer & Ehrend. Pollen grains in Succiseae are characterized by a tricolpate aperture type, which was probably ancestral in Dipsacaceae s. str.


Zootaxa ◽  
2021 ◽  
Vol 5030 (1) ◽  
pp. 1-118
Author(s):  
GRAEME B. SMITH ◽  
ANDREW MITCHELL ◽  
RAFAEL MOLERO-BALTANÁS

Molecular studies using COI and 28S sequence data strongly identify a clade within the Heterolepismatinae distinct from the majority of species so far sequenced. The independence of the clade is supported by several morphological characters including a glabrous anterior margin to the frons, large trapezoidal thoracic sternites, tarsal trichobothria, long, conical parameres which in some species consist of two segments, and the presence of triangular or rounded subrectangular scales on the femora, tibia and clypeus. This clade is described as a new genus Visma n. gen. containing ten new species V. advenum n. sp., V. bingara n. sp., V. brayi n. sp., V. bundjalung n. sp., V. brigalowsum n. sp., V. capricornia n. sp., V. pallidum n. sp., V. powellheueri n. sp., V. tenebrosum n. sp. and V. xanthorrhoea n. sp.. Heterolepisma stilivarians Silvestri, 1908 is redescribed from the holotype and transferred to the new genus. The remaining H. stilivarians type series is found to be different to the holotype and removed from the type series. It is considered possible that H. annectens Silvestri, 1924 may also belong to this genus. Scanning electron microscopy of scale shape and rib-spacing is shown to be a useful tool to separate at least some species of the genus and considered to have greater potential if well preserved material is available.  


Sign in / Sign up

Export Citation Format

Share Document