Pollen grain micromorphology in some genera of Ornithogaleae (Asparagaceae, Scilloideae) and its relative significance

Phytotaxa ◽  
2020 ◽  
Vol 458 (4) ◽  
pp. 242-256
Author(s):  
MEHDI HEIDARIAN ◽  
SEYED MOHAMMAD MAHDI HAMDI ◽  
MOHAMMAD MEHDI DEHSHIRI ◽  
TAHER NEJADSATTARI ◽  
SEYED MOHAMMAD MASOUMI
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Pollen Grains ◽  
Pollen Grain ◽  
Relative Significance ◽  
Phylogenetic Studies ◽  
Transmission Electron ◽  
Exine Ornamentation ◽  
Tem Transmission Electron Microscopy ◽  
Scanning Electron

In this research, the pollen micromorphology of nine species belonging to Honorius, Loncomelos, Ornithogalum and Stellarioides are observed by LM (Light Microscopy), SEM (Scanning Electron Microscopy) and two species by TEM (Transmission Electron Microscopy). Lately, the phylogenetic studies have determined nineteen monophyletic genera in subfamily Ornithogaloideae (=tribe Ornithogaleae). Our results show that the pollen grains characters such as shape, size, exine ornamentation on equatorial surface, sulcus margin exine ornamentation, sulcus membrane ornamentation and lumina number on exine surface are varied among all studied genera. The dendrogram of palynological characters analyzed on LM and SEM observation by MVSP (Multi Variate Statistical Package) software partly supports the monophyletic genera. According to the obtained palynological dendrogram, Loncomelos arcuatum and Ornithogalum sintenisii are placed in one subtype. Based on the sporoderm structure (exine thickness and the endexine type), L. arcuatum differs from O. sintenisii. Finally, the palynological characters can be useful for clarifying the taxonomic relationship in this subfamily.

Pollen morphology of some species belonging to Codonoprasum and Allium sections of Allium (Liliaceae-Alliaceae) genus

Biologia ◽  
2006 ◽  
Vol 61 (4) ◽  
Author(s):  
Ümmügülsüm Güler ◽  
Sevil Pehlivan
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Pollen Morphology ◽  
Morphological Characteristics ◽  
Transmission Electron ◽  
Exine Ornamentation ◽  
The Family ◽  
Tem Transmission Electron Microscopy ◽  
Scanning Electron ◽  
Section Level

AbstractPollen morphology of 14 Allium L. species grown in Turkey, that belong to the sections Codonoprasum and Allium, were investigated under LM (light microscopy) and by SEM (scanning electron microscopy). However, the pollens of 5 species were investigated under TEM (transmission electron microscopy). Detailed pollen morphological characteristics are given for Allium in the family on the basis of the results presented here together with data from the literature. The genera Allium homogeneous in both aperture type and exine ornamentation. It is suggested that some palynological characters, such as aperture type and the presence of an operculum, could be of taxonomic value at the section level.

Pollen morphology of Sabinaria magnifica (Cryosophileae, Coryphoideae, Arecaceae)

Phytotaxa ◽  
2015 ◽  
Vol 207 (1) ◽  
pp. 135 ◽  
Author(s):  
Giovanni Raul Bogota ◽  
Carina Hoorn ◽  
Wim Star ◽  
Rob Langelaan ◽  
Hannah Banks ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Light Microscopy ◽  
Pollen Morphology ◽  
Pollen Grains ◽  
The Other ◽  
Transmission Electron ◽  
Palm Species ◽  
Scanning Electron

Sabinaria magnifica is so far the only known species in the recently discovered tropical palm genus Sabinaria (Arecaceae). Here we present a complete description of the pollen morphology of this palm species based on light microscopy (LM), scanning electron microscopy (SEM) and transmission electron microscopy (TEM). We also made SEM-based comparisons of Sabinaria with other genera within the tribe Cryosophileae. Pollen grains of Sabinaria magnifica resemble the other genera in the heteropolar, slightly asymmetric monads, and the monosulcate and tectate exine with perforate surface. Nevertheless, there are some clear differences with Thrinax, Chelyocarpus and Cryosophila in terms of aperture and exine. S. magnifica differs from its closest relative, Itaya amicorum, in the exine structure. This study shows that a combination of microscope techniques is essential for the identification of different genera within the Cryosophileae and may also be a necessary when working with other palynologically less distinct palm genera. 

scholarly journals Pollen grain surface in Vaccinium myrtillus as seen in scanning electron microscopy

2014 ◽  
Vol 50 (4) ◽  
pp. 583-584
Author(s):  
Józef Kocoń ◽  
Kazimierz Pliszka ◽  
Stanisław Muszyński
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Pollen Grains ◽  
Pollen Grain ◽  
Vaccinium Myrtillus ◽  
Grain Surface ◽  
Scanning Electron

Pollen grain surface of <em>Vaccinium myrtillus</em> L. was analyzed by scanning electron microscopy. Pollen grains remain in tetrahedral tetrads. Grain surface is verrucose, consisting of thick, irregularly shaped muri, surrounding small, round or oval lumina. The surface of the muri is fissured, and minute papillae can also be noted.

scholarly journals Distinguishing Oblacinska sour cherry clones (Prunus cerasus L.) by pollen morphology

Genetika ◽  
2020 ◽  
Vol 52 (1) ◽  
pp. 187-198 ◽  
Author(s):  
Dragan Nikolic ◽  
Dragan Milatovic ◽  
Aleksandar Radovic ◽  
Jugoslav Trajkovic
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Pollen Morphology ◽  
Pollen Grains ◽  
Sour Cherry ◽  
Prunus Cerasus ◽  
Morphological Parameters ◽  
Exine Ornamentation ◽  
Pollen Shape ◽  
Scanning Electron

Morphology and ultrastructure of pollen grains were studied in 13 Obla?inska sour cherry clones in three years using scanning electron microscopy (SEM). All studied clones had isopolar, radially symmetric and tricolpate pollen grains. Length and width of pollen grains varied in a range of 50.03-55.97 ?m and 25.16-28.37 ?m respectively. Pollen shape in all the studied clones was identified as prolate. The smallest colpus length was found in the clone 8 (43.73 ?m), and the highest in the clone 3 (49.16 ?m). The highest colpus and mesocolpium width had the clone 3 (1.73 ?m; 14.87 ?m) and the lowest had the clone 7 (1.46 ?m; 13.78 ?m). All studied clones had striate exine ornamentation. Number of ridges per 100 ?m2 of the exine surface was the highest in the clone 11 (16.7) and lowest in the clone 2 (14.1). Ridge and furrow width ranged from 0.46 to 0.61 ?m and from 0.45 to 0.59 ?m, respectively. The clones have been classified into three clusters based on all studied properties. Pollen grains examination by SEM indicated that several morphological parameters (pollen size and exine characteristics) can be used to distinguish Obla?inska sour cherry clones.

scholarly journals Taxonomic significance of pollen morphology in Succisa and Succisella

2019 ◽  
Vol 55 (1) ◽  
pp. 1-6 ◽  
Author(s):  
Zoya M. Tsymbalyuk ◽  
Sergei L. Mosyakin ◽  
Lyudmila M. Nitsenko
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Pollen Morphology ◽  
Pollen Grains ◽  
Polar View ◽  
Taxonomic Significance ◽  
Oblate Spheroidal ◽  
Phylogenetic Studies ◽  
Molecular Phylogenetic ◽  
Scanning Electron

Abstract Pollen morphology of representatives of the genera Succisa and Succisella in the flora of Ukraine was studied using light and scanning electron microscopy. Pollen grains in both taxa were tricolpate, prolate, rarely oblate-spheroidal or spheroidal; large-sized. Their outline in polar view was subcircular, rarely trilobate, in equatorial view elliptical or rarely circular. Colpi short, of variable width, margins irregular with distinct, narrow margo, and blunt or acute ends. Exine sculpture was echinate-microechinate. The revealed characteristics of pollen grains are taxonomically significant at the generic and specific levels, and they can be used in pollen analysis. Palynomorphological data are consistent with the results of recent molecular phylogenetic studies. Data on pollen morphology also confirm taxonomic circumscription of Succiseae V. Mayer & Ehrend. Pollen grains in Succiseae are characterized by a tricolpate aperture type, which was probably ancestral in Dipsacaceae s. str.

Morphological, cytological, palynological and molecular evidence on two new hybrids from Turkey: an example of homoploid hybridization in Origanum (Lamiaceae)

Phytotaxa ◽  
2018 ◽  
Vol 371 (3) ◽  
pp. 145 ◽  
Author(s):  
TUNCAY DİRMENCİ ◽  
TANER ÖZCAN ◽  
TÜRKER YAZICI ◽  
TURAN ARABACI ◽  
ESRA MARTİN
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Somatic Chromosome ◽  
Pollen Grains ◽  
Molecular Evidence ◽  
Hybrid Swarms ◽  
Pollen Characters ◽  
Exine Ornamentation ◽  
Nuclear Its ◽  
Scanning Electron

Two new hybrids of Origanum, O. ×malyeri and O. ×sevcaniae, are described and illustrated in detail. The general morphology, pollen and chromosome features, and nuclear (ITS) and chloroplast (rpl32) genomes of collected specimens were studied. Origanum ×malyeri is a hybrid between O. boissieri and O. vulgare subsp. hirtum and O. ×sevcaniae is another hybrid between O. vulgare subsp. hirtum and O. vogelii. These two new hybrids have some morphological intermediate characters between their parents. The pollen morphology of O. ×sevcaniae and its parents were studied and documented in detail using light microscopy (LM) and scanning electron microscopy (SEM). O. ×sevcaniae pollen is small to medium (P=18.49–21.03 µm, E=22.20–26.63 µm), suboblate (73%) to oblate (22%), the range of P/E ratio is from 0.73 to 0.85. Pollen grains are hexacolpate with granular membranes. Pollen grains were identified as bireticulate exine ornamentation with scanning electron microscopy examinations. The somatic chromosome number of O. boissieri, O. vulgare subsp. hirtum, O. vogelii, and O. ×sevcaniae were counted as 2n = 30. Pollen characters and somatic chromosome numbers of O. ×malyeri could not be examined because it has only female flowers and has not any nutlets. Also, according to molecular results, O. ×malyeri and O. ×sevcaniae have some polymorphic nucleotide regions in their nuclear ITS genomes and which means they have two different DNA dataset belonging to their parents. Also, some individuals of O. ×sevcaniae parents have single nucleotide polymorphism, too. These individuals live together with their hybrids and hybridization is probably a continuing process in that hybrid swarms. These present results support previous reports for the speciation of Origanum members via hybridization.

scholarly journals Taxonomical Investigation on Some Species of Genus Allium Based on the Pollen Morphology

2019 ◽  
pp. 1-10
Author(s):  
Mehdi Heidarian ◽  
Seyed Mohammad Mehdi Hamdi ◽  
Mohammad Mehdi Dehshiri ◽  
Taher Nejadsattari ◽  
Seyed Mohammad Masoumi
Keyword(s):  
Electron Microscopy ◽  
Pollen Morphology ◽  
Numerical Taxonomy ◽  
Morphological Characteristics ◽  
Pollen Grains ◽  
Pollen Grain ◽  
Wall Structure ◽  
Transmission Electron ◽  
Pollen Characters ◽  
Exine Ornamentation

Aims: The aim of this research was to investigate the morphological characteristics of the pollen grain of some species of genus Allium. Methodology: The pollen grains were examined by using Light Microscopy (LM), Scanning Electron Microscopy (SEM) and the pollen grains of one species under Transmission Electron Microscopy (TEM). Results: The pollen grains were oblate and medium in shape and size. The pollen ornamentation of exine surface, exine ornamentation on sulcus edge, lumina number in the exine surface and the state of pollen grain apex in the examined species were different. Semitectate and columellate ectexine with discontinuous endexine were seen in the pollen wall structure (sporoderm). The dendrogram obtained from the pollen characters in SEM observations by using the numerical taxonomy system (NTSYS) software placed the studied species in two types. Conclusion: Our palynological dendrogram can be used for segregation the sections and subgenera taxonomical levels in the studied species of genus Allium and confirmed the phylogram of the recent phylogenetic research.

scholarly journals Pollen Morphology of Pinus mugo Turra × Pinus sylvestris L. Hybrids and Parental Species in an Experimental Culture

2015 ◽  
Vol 57 (1) ◽  
pp. 149-160 ◽  
Author(s):  
Joanna Bykowska ◽  
Małgorzata Klimko
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Pollen Morphology ◽  
Parental Species ◽  
Pollen Grains ◽  
Grain Morphology ◽  
Pollen Grain ◽  
Pinus Mugo ◽  
Pollen Grain Morphology ◽  
Scanning Electron

Abstract Pollen grains of Pinus mugo, P. sylvestris and P. ×rhaetica (= P. mugo × P. sylvestris) were examined by light and scanning electron microscopy. The pollen grains were bisaccate and monosulcate. The corpus-saccus attachment was distinct. The pollen corpus exine sculpture was verrucate-rugulate and deeply sculptured. The surface of the tectum was covered with small grana and it was perforate. The saccus sexine ornamentation was reticulate and irregularly perforate. The tectum surface characters in the proximal and distal view of the corpus and saccus were less variable and they did not provide good criteria to identify the species under study. However, some differences were observed in the size of elevation on the corpus between pollen grains of the same species and between the parental species. This study of the pollen grain morphology of the corpus and saccus provided some important new data.

Morphology, anatomy and palynology study on Turkish endemic species Saponaria karapinarensis (Caryophyllaceae)

Phytotaxa ◽  
2018 ◽  
Vol 374 (1) ◽  
pp. 80
Author(s):  
DENİZ ULUKUŞ
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Endemic Species ◽  
Pollen Grains ◽  
Point Of View ◽  
Anatomical Features ◽  
Exine Ornamentation ◽  
Cell Layers ◽  
Taxonomic Point ◽  
Scanning Electron

Saponaria karapinarensis (Caryophyllaceae), an endemic species from Turkey, was studied from taxonomic point of view. Morphological, anatomical, and palynological characteristics of the species were examined. Morphology of stem, calyx, petals, and capsule are useful to distinguish the species. Anatomical features, such as the number of cortex layers and sclerenchymatic cell layers in stem, are of taxonomical importance. SEM Scanning Electron Microscopy (SEM) studies on the pollen grains have revealed that they are spheroideae and their exine ornamentation is granulatae, spinulate microechinatae- microperforatae. Seed micromorphological features were also given.

Inheritance of exine ornamentation and pollen shape in the interspecific tetraploid hybrids of Gloriosa

1986 ◽  
Vol 64 (12) ◽  
pp. 3134-3140 ◽  
Author(s):  
C. Ravi kumar ◽  
P. K. K. Nair
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Light Microscopy ◽  
Maternal Inheritance ◽  
Parental Species ◽  
Pollen Grains ◽  
The Other ◽  
Exine Ornamentation ◽  
Pollen Shape ◽  
Scanning Electron

Scanning electron microscopy and light microscopy of the pollen of three tetraploid (2n = 44) species, Gloriosa virescens Lindle, G. carsonii Baker, and G. richmondensis Hort., and three of their hybrids showed that the pollen in Gloriosa L. fundamentally consists of three pollen shape types, spheroidal, ellipsoidal, and spindle shaped, having monocolpate aperture and a striate exine ornamentation pattern. The three parental species can be differentiated from each other on the basis of pollen shape and their characteristic striate exine ornamentation. However, in all three hybrids the composition of pollen shape suggests maternal inheritance of the character. Furthermore, in each of the three hybrids, new exine ornamentation features have appeared which differ from those of the other hybrids and from their respective parents. All the pollen grains in each of the hybrids are uniform with the same characteristic exine ornamentation. They show no segregation of exine features into the parental categories, which would be expected if exine ornamentation was under sporophytic control. The potential of exine features as genetic markers in hybrids is discussed.

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