Hermatomyces trangensis sp. nov., a new dematiaceous hyphomycete (Hermatomycetaceae, Pleosporales) on sugar palm in Thailand

A new species of a hyphomycetous fungus, Hermatomyces trangensis, was collected during an investigation of the diversity of palm fungi in Na Yong district, Trang province, Southern Thailand, and is introduced in this paper based on morphological and molecular evidence. The fungus is characterized by the production of only one type of conidia, globose or subglobose in front view, broadly ellipsoidal or oblong in lateral view, with dark brown to black central cells and subhyaline to pale brown peripheral cells and sporulation in culture. Phylogenetic analyses of combined SSU rDNA, LSU rDNA, ITS rDNA, TEF1 and RPB2 sequence data using maximum parsimony, maximum likelihood and Bayesian inference approaches placed the fungus within a strongly supported clade with other Hermatomyces species within the family Hermatomycetaceae (Pleosporales, Dothideomycetes). Morphologically, it is similar to other taxa with only one type of conidia such as H. sphaericus, H. sphaericoides and H. verrucosus but molecular data clearly support H. trangensis as distinct from those species.

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Tamhinispora saraburiensis sp. nov. (Tubeufiaceae, Dothideomycetes) on bamboo in Thailand based on morphology & phylogenetic analysis

Phytotaxa ◽

10.11646/phytotaxa.402.1.1 ◽

2019 ◽

Vol 402 (1) ◽

pp. 1 ◽

Cited By ~ 1

Author(s):

SUJINDA SOMMAI ◽

SALILAPORN NUANKAEW ◽

PHONGSWAT KHAMSUNTORN ◽

SATINEE SUETRONG ◽

UMPAWA PINRUAN

Keyword(s):

Phylogenetic Analysis ◽

Sequence Data ◽

Phylogenetic Analyses ◽

Molecular Data ◽

Rdna Sequence ◽

Lsu Rdna ◽

The Family ◽

Rdna Sequence Data

Tamhinispora saraburiensis sp. nov. was collected on decaying culms of bamboo in Saraburi Province, Thailand. Tamhinispora saraburiensis is characterized as dark brown to black, obpyriform, and dictyoseptate and has conidia with long appendages arising from the conidial tip. Phylogenetic analyses of the combined ITS and LSU rDNA sequence data demonstrated that this species formed distinctly from other species and that it belongs in the family Tubeufiaceae. Tamhinispora saraburiensis is therefore introduced here with a description, morphological illustrations and molecular data. A key to the species of Tamhinispora is provided.

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Generic delimitations in the family Stictidaceae (Ostropales, Ascomycota): the Stictis–Conotrema problem

The Lichenologist ◽

10.1017/s0024282904014653 ◽

2005 ◽

Vol 37 (1) ◽

pp. 67-75 ◽

Cited By ~ 18

Author(s):

Mats WEDIN ◽

Heidi DÖRING ◽

Kristina KÖNBERG ◽

Gunnar GILENSTAM

Keyword(s):

Sequence Data ◽

Sister Group ◽

Lsu Rdna ◽

Infrageneric Classification ◽

Rdna Its ◽

The Family ◽

Rdna Sequence Data ◽

Natural Groups ◽

High Support

The family Stictidaceae (Ostropales, Ascomycota) contains both lichenized and non-lichenized fungi. Here, we test if Conotrema (lichenized) and Stictis (non-lichenized) as currently delimited are distinct monophyletic genera, by parsimony and parsimony jackknifing analyses of combined nuclear rDNA (ITS and partial LSU rDNA) and mitochondrial SSU rDNA sequence data matrices. The study includes four species of Stictis, three species of Conotrema, and representatives of the related Schizoxylon (lichenized), Odontotrema, Carestiella (at least sometimes associated with algae), Cryptodiscus and Thelotrema (lichenized). In all analyses, the Conotrema species were nested within Stictis with high support. Thus, we conclude that Conotrema are only lichenized representatives of Stictis. The type species of the two generic names, C. urceolatum and S. radiata, are sister taxa in our analyses. Furthermore, the analysis gave no support for the present infrageneric classification of Stictis. Carestiella socia (the type of Carestiella) and the two representatives of Schizoxylon studied were also nested within Stictis s. lat. The Odontotremataceae is the sister group to the Stictidaceae, and Cryptodiscus foveolaris groups with Thelotrema rather than with the Stictidaceae. We conclude that lichenization in the Stictidaceae does not characterize natural groups, and that Conotrema should be considered a synonym to Stictis, as predicted by anatomical characteristics. The new combinations Stictis urceolatum and Stictis populorum are made.

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The genus Coprinellus (Basidiomycota; Agaricales) in Pakistan with the description of four new species

MycoKeys ◽

10.3897/mycokeys.39.26743 ◽

2018 ◽

Vol 39 ◽

pp. 41-61 ◽

Cited By ~ 3

Author(s):

Shah Hussain ◽

Muhammad Usman ◽

Najam-ul-Sehar Afshan ◽

Habib Ahmad ◽

Junaid Khan ◽

...

Keyword(s):

New Species ◽

Phylogenetic Analyses ◽

Its Region ◽

Molecular Data ◽

Species Level ◽

Rdna Its ◽

The Family ◽

Rdna Its Region ◽

Morphological And Molecular Data ◽

Species Descriptions

Mushrooms with a thin-fleshed pileus that becomes plicate on opening, deliquescent lamellae and dark brown to blackish basidiospores are commonly called coprinoid mushrooms. The genusCoprinellusis one of the important lineages of coprinoid mushroom in the family Psathyrellaceae. Species-level taxonomy inCoprinellusis based mainly on the presence or absence and the structure of veil and cystidia on the pileus, of cystidia on the lamellae and on basidiospore morphology. In this study, four new species ofCoprinellus(Co.campanulatus,Co.disseminatus-similis,Co.pakistanicusandCo.tenuis) are described from Pakistan. Species descriptions are based on morphological and molecular data. Phylogenetic analyses based on nuc rDNA ITS region show that the new speciesCo.campanulatusandCo.disseminatus-similisare clustered in a clade including members of section Micacei;Co.tenuisfalls in a clade with members of section Domestici; andCo.pakistanicusrecovered in a separate clade adjacent to other recently described clades of genusCoprinellus. Morpho-anatomical descriptions of the new species and comparison with closely allied taxa are provided. With this study, the number of known species ofCoprinellusin Pakistan has reached eight.

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Discovery of Novel Backusella (Backusellaceae, Mucorales) Isolated from Invertebrates and Toads in Cheongyang, Korea

Journal of Fungi ◽

10.3390/jof7070513 ◽

2021 ◽

Vol 7 (7) ◽

pp. 513

Author(s):

Thuong T.T. Nguyen ◽

Kerstin Voigt ◽

André Luiz Cabral Monteiro de Azevedo Santiago ◽

Paul M. Kirk ◽

Hyang-Burm Lee

Keyword(s):

Ribosomal Dna ◽

Sequence Data ◽

Phylogenetic Analyses ◽

Large Subunit ◽

Morphological Characters ◽

Fungal Species ◽

Lsu Rdna ◽

Rdna Sequences ◽

The Family ◽

Internal Transcribed Spacer Sequences

Three novel fungal species, Backusella chlamydospora sp. nov., B. koreana sp. nov., and B. thermophila sp. nov., as well as two new records, B. oblongielliptica and B. oblongispora, were found in Cheongyang, Korea, during an investigation of fungal species from invertebrates and toads. All species are described here using morphological characters and sequence data from internal transcribed spacer sequences of ribosomal DNA and large subunit of the ribosomal DNA. Backusella chlamydospora is different from other Backusella species by producing chlamydospores. Backusella koreana can be distinguished from other Backusella species by producing abundant yeast-like cells. Backusella thermophila is characterized by a variable (subglobose to oblong, applanate to oval, conical and ellipsoidal to pyriform) columellae and grows well at 37 °C. Multigene phylogenetic analyses of the combined ITS and LSU rDNA sequences data generated from maximum likelihood and MrBayes analyses indicate that B. chlamydospora, B. koreana, and B. thermophila form distinct lineages in the family Backusellaceae. Detailed descriptions, illustrations, phylogenetic tree, and taxonomic key to the Backusella species present in Korea are provided.

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The (non) accuracy of mitochondrial genomes for family level phylogenetics: the case of erebid moths (Lepidoptera; Erebidae)

10.1101/2021.07.14.452330 ◽

2021 ◽

Author(s):

Hamid Reza Ghanavi ◽

Victoria Twort ◽

Tobias Joannes Hartman ◽

Reza Zahiri ◽

Niklas Wahlberg

Keyword(s):

Mitochondrial Dna ◽

Phylogenetic Relationships ◽

High Throughput Sequencing ◽

Sequence Data ◽

Phylogenetic Analyses ◽

Molecular Data ◽

Mitochondrial Genomes ◽

The Family ◽

History Of ◽

Genomic Scale

The use of molecular data to study evolutionary history of different organisms, revolutionized the field of systematics. Now with the appearance of high throughput sequencing (HTS) technologies more and more genetic sequence data is available. One of the important sources of genetic data for phylogenetic analyses has been mitochondrial DNA. The limitations of mitochondrial DNA for the study of phylogenetic relationships have been thoroughly explored in the age of single locus phylogenies. Now with the appearance of genomic scale data, more and more mitochondrial genomes are available. Here we assemble 47 mitochondrial genomes using whole genome Illumina short reads of representatives of the family Erebidae (Lepidoptera), in order to evaluate the accuracy of mitochondrial genome application in resolving deep phylogenetic relationships. We find that mitogenomes are inadequate for resolving subfamily level relationships in Erebidae, but given good taxon sampling, we see its potential in resolving lower level phylogenetic relationships.

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Evidence from the resurrected family Polyrhabdinidae Kamm, 1922 (Apicomplexa: Gregarinomorpha) supports the epimerite, an attachment organelle, as a major eugregarine innovation

PeerJ ◽

10.7717/peerj.11912 ◽

2021 ◽

Vol 9 ◽

pp. e11912

Author(s):

Gita G. Paskerova ◽

Tatiana S. Miroliubova ◽

Andrea Valigurová ◽

Jan Janouškovec ◽

Magdaléna Kováčiková ◽

...

Keyword(s):

Electron Microscopy ◽

Light Microscopy ◽

Dna Sequences ◽

Phylogenetic Analyses ◽

Strong Support ◽

Molecular Data ◽

Lsu Rdna ◽

The Family ◽

Molecular Phylogenetic ◽

And Function

Background Gregarines are a major group of apicomplexan parasites of invertebrates. The gregarine classification is largely incomplete because it relies primarily on light microscopy, while electron microscopy and molecular data in the group are fragmentary and often do not overlap. A key characteristic in gregarine taxonomy is the structure and function of their attachment organelles (AOs). AOs have been commonly classified as “mucrons” or “epimerites” based on their association with other cellular traits such as septation. An alternative proposal focused on the AOs structure, functional role, and developmental fate has recently restricted the terms “mucron” to archigregarines and “epimerite” to eugregarines. Methods Light microscopy and scanning and transmission electron microscopy, molecular phylogenetic analyses of ribosomal RNA genes. Results We obtained the first data on fine morphology of aseptate eugregarines Polyrhabdina pygospionis and Polyrhabdina cf. spionis, the type species. We demonstrate that their AOs differ from the mucron in archigregarines and represent an epimerite structurally resembling that in other eugregarines examined using electron microscopy. We then used the concatenated ribosomal operon DNA sequences (SSU, 5.8S, and LSU rDNA) of P. pygospionis to explore the phylogeny of eugregarines with a resolution superior to SSU rDNA alone. The obtained phylogenies show that the Polyrhabdina clade represents an independent, deep-branching family in the Ancoroidea clade within eugregarines. Combined, these results lend strong support to the hypothesis that the epimerite is a synapomorphic innovation of eugregarines. Based on these findings, we resurrect the family Polyrhabdinidae Kamm, 1922 and erect and diagnose the family Trollidiidae fam. n. within the superfamily Ancoroidea Simdyanov et al., 2017. Additionally, we re-describe the characteristics of P. pygospionis, emend the diagnoses of the genus Polyrhabdina, the family Polyrhabdinidae, and the superfamily Ancoroidea.

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A new view on the morphology and phylogeny of eugregarines suggested by the evidence from the gregarine Ancora sagittata (Leuckart, 1860) Labbé, 1899 (Apicomplexa: Eugregarinida)

PeerJ ◽

10.7717/peerj.3354 ◽

2017 ◽

Vol 5 ◽

pp. e3354 ◽

Cited By ~ 15

Author(s):

Timur G. Simdyanov ◽

Laure Guillou ◽

Andrei Y. Diakin ◽

Kirill V. Mikhailov ◽

Joseph Schrével ◽

...

Keyword(s):

Electron Microscopy ◽

Phylogenetic Trees ◽

Sequence Data ◽

Phylogenetic Analyses ◽

Molecular Data ◽

Wide Distribution ◽

Phylogenetic Study ◽

Molecular Evidence ◽

Molecular Phylogenetic ◽

Phylogenetic Lineages

Background Gregarines are a group of early branching Apicomplexa parasitizing invertebrate animals. Despite their wide distribution and relevance to the understanding the phylogenesis of apicomplexans, gregarines remain understudied: light microscopy data are insufficient for classification, and electron microscopy and molecular data are fragmentary and overlap only partially. Methods Scanning and transmission electron microscopy, PCR, DNA cloning and sequencing (Sanger and NGS), molecular phylogenetic analyses using ribosomal RNA genes (18S (SSU), 5.8S, and 28S (LSU) ribosomal DNAs (rDNAs)). Results and Discussion We present the results of an ultrastructural and molecular phylogenetic study on the marine gregarine Ancora sagittata from the polychaete Capitella capitata followed by evolutionary and taxonomic synthesis of the morphological and molecular phylogenetic evidence on eugregarines. The ultrastructure of Ancora sagittata generally corresponds to that of other eugregarines, but reveals some differences in epicytic folds (crests) and attachment apparatus to gregarines in the family Lecudinidae, where Ancora sagittata has been classified. Molecular phylogenetic trees based on SSU (18S) rDNA reveal several robust clades (superfamilies) of eugregarines, including Ancoroidea superfam. nov., which comprises two families (Ancoridae fam. nov. and Polyplicariidae) and branches separately from the Lecudinidae; thus, all representatives of Ancoroidea are here officially removed from the Lecudinidae. Analysis of sequence data also points to possible cryptic species within Ancora sagittata and the inclusion of numerous environmental sequences from anoxic habitats within the Ancoroidea. LSU (28S) rDNA phylogenies, unlike the analysis of SSU rDNA alone, recover a well-supported monophyly of the gregarines involved (eugregarines), although this conclusion is currently limited by sparse taxon sampling and the presence of fast-evolving sequences in some species. Comparative morphological analyses of gregarine teguments and attachment organelles lead us to revise their terminology. The terms “longitudinal folds” and “mucron” are restricted to archigregarines, whereas the terms “epicystic crests” and “epimerite” are proposed to describe the candidate synapomorphies of eugregarines, which, consequently, are considered as a monophyletic group. Abolishing the suborders Aseptata and Septata, incorporating neogregarines into the Eugregarinida, and treating the major molecular phylogenetic lineages of eugregarines as superfamilies appear as the best way of reconciling recent morphological and molecular evidence. Accordingly, the diagnosis of the order Eugregarinida Léger, 1900 is updated.

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The genus Lolia from freshwater habitats in Egypt with one new species

Phytotaxa ◽

10.11646/phytotaxa.267.4.4 ◽

2016 ◽

Vol 267 (4) ◽

pp. 279 ◽

Cited By ~ 2

Author(s):

FATEN A. ABDEL-AZIZ

Keyword(s):

New Species ◽

Sequence Data ◽

Phylogenetic Analyses ◽

Molecular Data ◽

Rdna Sequence ◽

Lsu Rdna ◽

Sexual Morph ◽

Molecular Phylogenetic ◽

Freshwater Habitats ◽

A New Species

A new species of Lolia and the sexual morph of L. aquatica are described, with illustrations, from decayed submerged wood in the River Nile, Egypt. Molecular phylogenetic analyses of partial SSU and LSU rDNA sequence data place the sexual morph of L. aquatica and the new species, L. dictyospora in a monophyletic clade, distant from the closely related fungi in the freshwater family Lindgomycetaceae. Pairwise LSU rDNA sequence alignment of the sexual/asexual morphs of L. aquatica are 99% similar. Lolia species are characterized by pseudothecial or stromatic, immersed to erumpent, dark-brown to black, coriaceous to sub-carbonaceous ascomata, cylindric-clavate, bitunicate, fissitunicate, short pedicellate asci, yellow to reddish-brown, phragmo- or dictyospores, surrounded by prominent gelatinous sheath. Lolia species are phylogenetically distant from the closely related genera: Leptosphaeria (Leptosphaeriaceae) and Massariosphaeria (Pleosporales incert. sed.). Lolia differs from Leptosphaeria in having a prominent large gelatinous ascospore sheath, while this is absent in Leptosphaeria species. Lolia differs from Massariosphaeria in having smaller ascospores and fewer transverse septa. Based on molecular data and morphology, a new species, Lolia dictyospora, is established.

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Integrative taxonomy and molecular phylogeny of three poorly known tintinnine ciliates, with the establishment of a new genus (Protista; Ciliophora; Oligotrichea)

BMC Ecology and Evolution ◽

10.1186/s12862-021-01831-8 ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Rui Wang ◽

Yang Bai ◽

Tao Hu ◽

Dapeng Xu ◽

Toshikazu Suzuki ◽

...

Keyword(s):

Phylogenetic Trees ◽

New Genus ◽

Sequence Data ◽

Phylogenetic Analyses ◽

Molecular Data ◽

Integrative Taxonomy ◽

Habitat Characteristics ◽

Lsu Rdna ◽

Molecular Phylogenetic ◽

Ciliary Tuft

Abstract Background The taxonomic classification of the suborder Tintinnina Kofoid & Campbell, 1929, a species-rich group of planktonic ciliated protistans with a characteristic lorica, has long been ambiguous largely due to the lack of cytological and molecular data for most species. Tintinnopsis is the largest, most widespread, and most taxonomically complex genus within this group with about 170 species occurring in nearshore waters. Previous molecular phylogenetic studies have revealed that Tintinnopsis is polyphyletic. Results Here we document the live morphology, infraciliature, gene sequences, and habitat characteristics of three poorly known tintinnine species, viz. Tintinnopsis karajacensis Brandt, 1896, Tintinnopsis gracilis Kofoid & Campbell, 1929, and Tintinnopsis tocantinensis Kofoid & Campbell, 1929, isolated from the coastal waters of China. Based on a unique cytological feature (i.e., an elongated ciliary tuft with densely arranged kinetids) in the former two species, Antetintinnopsis gen. nov. is erected with Antetintinnopsis hemispiralis (Yin, 1956) comb. nov. (original combination: Tintinnopsis hemispiralis Yin, 1956) designated as the type species. Moreover, A. karajacensis (Brandt, 1896) comb. nov. (original combination: Tintinnopsis karajacensis Brandt, 1896) and A. gracilis (Kofoid & Campbell, 1929) comb. nov. (original combination: Tintinnopsis gracilis Kofoid & Campbell, 1929) are placed in a highly supported clade that branches separately from Tintinnopsis clades in phylogenetic trees based on SSU rDNA and LSU rDNA sequence data, thus supporting the establishment of the new genus. One other species is assigned to Antetintinnopsis gen. nov., namely A. subacuta (Jörgensen, 1899) comb. nov. (original combination Tintinnopsis subacuta Jörgensen, 1899). Conclusions The findings of the phylogenetic analyses support the assertion that cytological characters are taxonomically informative for tintinnines. This study also contributes to the broadening of our understanding of the tintinnine biodiversity and evolution.

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Loose Ends in the Cortinarius Phylogeny: Five New Myxotelamonoid Species Indicate a High Diversity of These Ectomycorrhizal Fungi with South American Nothofagaceae

Life ◽

10.3390/life11050420 ◽

2021 ◽

Vol 11 (5) ◽

pp. 420

Author(s):

María Eugenia Salgado Salomón ◽

Carolina Barroetaveña ◽

Tuula Niskanen ◽

Kare Liimatainen ◽

Matthew E. Smith ◽

...

Keyword(s):

New Species ◽

Current Knowledge ◽

Phylogenetic Analyses ◽

Molecular Data ◽

Global Scale ◽

South American ◽

Edible Fungi ◽

Rdna Its ◽

Morphological And Molecular Data ◽

High Diversity

This paper is a contribution to the current knowledge of taxonomy, ecology and distribution of South American Cortinarius (Pers.) Gray. Cortinarius is among the most widely distributed and species-rich basidiomycete genera occurring with South American Nothofagaceae and species are found in many distinct habitats, including shrublands and forests. Due to their ectomycorrhizal role, Cortinarius species are critical for nutrient cycling in forests, especially at higher latitudes. Some species have also been reported as edible fungi with high nutritional quality. Our aim is to unravel the taxonomy of selected Cortinarius belonging to phlegmacioid and myxotelamonioid species based on morphological and molecular data. After widely sampling Cortinarius specimens in Patagonian Nothofagaceae forests and comparing them to reference collections (including holotypes), we propose five new species of Cortinarius in this work. Phylogenetic analyses of concatenated rDNA ITS-LSU and RPB1 sequences failed to place these new species into known Cortinarius sections or lineages. These findings highlight our knowledge gaps regarding the fungal diversity of South American Nothofagaceae forests. Due to the high diversity of endemic Patagonian taxa, it is clear that the South American Cortinarius diversity needs to be discovered and described in order to understand the evolutionary history of Cortinarius on a global scale.

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