scholarly journals Pollen grain surface in Vaccinium myrtillus as seen in scanning electron microscopy

2014 ◽  
Vol 50 (4) ◽  
pp. 583-584
Author(s):  
Józef Kocoń ◽  
Kazimierz Pliszka ◽  
Stanisław Muszyński
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Pollen Grains ◽  
Pollen Grain ◽  
Vaccinium Myrtillus ◽  
Grain Surface ◽  
Scanning Electron

Pollen grain surface of <em>Vaccinium myrtillus</em> L. was analyzed by scanning electron microscopy. Pollen grains remain in tetrahedral tetrads. Grain surface is verrucose, consisting of thick, irregularly shaped muri, surrounding small, round or oval lumina. The surface of the muri is fissured, and minute papillae can also be noted.

scholarly journals Pollen Morphology of Pinus mugo Turra × Pinus sylvestris L. Hybrids and Parental Species in an Experimental Culture

2015 ◽  
Vol 57 (1) ◽  
pp. 149-160 ◽  
Author(s):  
Joanna Bykowska ◽  
Małgorzata Klimko
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Pollen Morphology ◽  
Parental Species ◽  
Pollen Grains ◽  
Grain Morphology ◽  
Pollen Grain ◽  
Pinus Mugo ◽  
Pollen Grain Morphology ◽  
Scanning Electron

Abstract Pollen grains of Pinus mugo, P. sylvestris and P. ×rhaetica (= P. mugo × P. sylvestris) were examined by light and scanning electron microscopy. The pollen grains were bisaccate and monosulcate. The corpus-saccus attachment was distinct. The pollen corpus exine sculpture was verrucate-rugulate and deeply sculptured. The surface of the tectum was covered with small grana and it was perforate. The saccus sexine ornamentation was reticulate and irregularly perforate. The tectum surface characters in the proximal and distal view of the corpus and saccus were less variable and they did not provide good criteria to identify the species under study. However, some differences were observed in the size of elevation on the corpus between pollen grains of the same species and between the parental species. This study of the pollen grain morphology of the corpus and saccus provided some important new data.

scholarly journals The ultrastructure of pollen grain surface in allotetraploid petunia (Petunia hybrida hort. superbissima) as revealed by scanning electron microscopy

2015 ◽  
Vol 46 (4) ◽  
pp. 599-601
Author(s):  
S. Muszyński ◽  
J. Kocoń ◽  
W. Guzowski ◽  
M. Bieguński
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Petunia Hybrida ◽  
Pollen Grain ◽  
Characteristic Pattern ◽  
Grain Surface ◽  
Pollen Grain Wall ◽  
Scanning Electron

The ultrastructure of pollen grain surface in allotetraploid petunias was analyzed by scanning electron microscopy. The pollen grain wall is developed into characteristic pattern of convulations.

Fine Structure of Pollen Grain Ektexine of Maize, Teosinte and Tripsacum

1972 ◽  
Vol 30 ◽  
pp. 226-227
Author(s):  
Umesh C. Banerjee ◽  
Elso S. Barghoorn
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Zea Mays ◽  
Fine Structure ◽  
Light Microscope ◽  
Pollen Grains ◽  
Pollen Grain ◽  
Wild Relatives ◽  
Distilled Water ◽  
Scanning Electron

Using light microscope (LM) it was found very difficult to distinguish the ektexine (outer sculptured layer of exine) pattern of maize (Zea mays L.) pollen grains from that of its wild relatives teosinte (Euchlaena mexicana Schrad.) and tripsacum (Tripsacum spp.). At the magnifications obtained by LM, the pollen grain ektexine is faintly granular or netted. By the use of electron microscopy, however, it is possible to characterize their pollen ektexine patterns.For scanning electron microscopy (SEM) acetolysed pollen grains were used. After acetolysis the pollen samples were washed several times in glass-distilled water to remove traces of acids. Each sample was dispersed in a drop of distilled water placed on specimen holders.

Pollen grain micromorphology in some genera of Ornithogaleae (Asparagaceae, Scilloideae) and its relative significance

Phytotaxa ◽  
2020 ◽  
Vol 458 (4) ◽  
pp. 242-256
Author(s):  
MEHDI HEIDARIAN ◽  
SEYED MOHAMMAD MAHDI HAMDI ◽  
MOHAMMAD MEHDI DEHSHIRI ◽  
TAHER NEJADSATTARI ◽  
SEYED MOHAMMAD MASOUMI
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Pollen Grains ◽  
Pollen Grain ◽  
Relative Significance ◽  
Phylogenetic Studies ◽  
Transmission Electron ◽  
Exine Ornamentation ◽  
Tem Transmission Electron Microscopy ◽  
Scanning Electron

In this research, the pollen micromorphology of nine species belonging to Honorius, Loncomelos, Ornithogalum and Stellarioides are observed by LM (Light Microscopy), SEM (Scanning Electron Microscopy) and two species by TEM (Transmission Electron Microscopy). Lately, the phylogenetic studies have determined nineteen monophyletic genera in subfamily Ornithogaloideae (=tribe Ornithogaleae). Our results show that the pollen grains characters such as shape, size, exine ornamentation on equatorial surface, sulcus margin exine ornamentation, sulcus membrane ornamentation and lumina number on exine surface are varied among all studied genera. The dendrogram of palynological characters analyzed on LM and SEM observation by MVSP (Multi Variate Statistical Package) software partly supports the monophyletic genera. According to the obtained palynological dendrogram, Loncomelos arcuatum and Ornithogalum sintenisii are placed in one subtype. Based on the sporoderm structure (exine thickness and the endexine type), L. arcuatum differs from O. sintenisii. Finally, the palynological characters can be useful for clarifying the taxonomic relationship in this subfamily.

scholarly journals Pollen morphology of the genus Eremanthus Less. (Vernonieae, Asteraceae)

2012 ◽  
Vol 26 (1) ◽  
pp. 46-57 ◽  
Author(s):  
BenoÎt Loeuille ◽  
Raquel Maria Batista Souza-Souza ◽  
Vanessa Holanda Righetti Abreu ◽  
Cláudia Barbieri Ferreira Mendonça ◽  
Vania Gonçalves-Esteves
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Pollen Morphology ◽  
Additional Data ◽  
Phylogenetic Reconstruction ◽  
Pollen Grains ◽  
Prolate Spheroidal ◽  
Oblate Spheroidal ◽  
Quantitative Characters ◽  
Scanning Electron

In order to evaluate the significance of the pollen morphology for generic and infrageneric taxonomy of the genus Eremanthus (Vernonieae, Asteraceae), and to provide additional data for its phylogenetic reconstruction, the pollen of 20 of the 23 species of the genus was examined using light and scanning electron microscopy. Acetolysed pollen grains were measured, described, and illustrated using light microscopy, while non-acetolysed pollen grains were analyzed using scanning electron microscopy. Pollen grains of these species are isopolar, oblate-spheroidal in most of the species, more rarely prolate spheroidal or suboblate, subtriangular amb, tricolporate and subechinolophate. The variation among quantitative characters does not correlate with the macromorphological subdivision of the genus or with the generic or specific limits.

scholarly journals Pollen morphology of Ukrainian species of the genus Pedicularis L. (Orobanchaceae Vent.)

2012 ◽  
Vol 24 (1) ◽  
pp. 5-13 ◽  
Author(s):  
Olena M. Peregrym ◽  
Zoya M. Tsymbalyuk ◽  
Sergei L. Mosyakin
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Pollen Morphology ◽  
Pollen Grains ◽  
Taxonomic Revision ◽  
Palynological Data ◽  
First Time ◽  
Scanning Electron

Abstract Pollen grains of 10 species of Pedicularis occurring in Ukraine were investigated using light and scanning electron microscopy. Palynological data on 6 species are reported for the first time. General pollen morphology of the genus Pedicularis is presented. Three types of apertures (2-syncolpate, 3-colpate, and 3-syncolpate) and seven subtypes of sculpture (3-syncolpate pilate, 3-syncolpate microscabrate-tubeculate, 2-syncolpate microscabrate, 2-syncolpate microscabrate-tubeculate, 2-syncolpate microscabrate-tubeculate-perforate, 2-syncolpate microfoveolate, and 2-syncolpate microscabrate-perforate) are indentified. The subgenus Pedicularis is heterogeneous in its types of apertures and sculpture of the surface, which indicates the need of further taxonomic revision of the group.

scholarly journals Pollen morphology of the Balkan-Carpathian endemic Campanula lingulata Waldst. & Kit. (Campanulaceae)

2016 ◽  
pp. 75-82
Author(s):  
Marina Macukanovic-Jocic ◽  
Snezana Jaric
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Light Microscopy ◽  
Endemic Species ◽  
Pollen Morphology ◽  
Average Length ◽  
Pollen Grains ◽  
Polar Axis ◽  
First Time ◽  
Scanning Electron

Palynomorphological characteristics of Campanula lingulata, the Balkan-Carpathian endemic species growing in Serbia, have been investigated using light microscopy and scanning electron microscopy for the first time, in order to provide some information helpful for a better understanding of the taxonomic position of this species within the genus, as well as to contribute to the pollen atlas of Serbian apiflora. The pollen grains are radially symmetrical, isopolar, 3-zonoporate and medium-sized monads oblate-sphaeroidal in shape. Mean of the polar axis (P) is 27.6?1.9 ?m, while the average length of the equatorial axis (E) is 28.8?1.6 ?m. The apertures are operculate. The sculpturing pattern of the exine is microre?ticulate-microechinatae. The exine surface is covered with evenly distributed supratectal spinules of variable length and sparse granules. The longest supratectal spinules are 0.64?0.05 ?m in length and the smallest sculptural elements are less than 0.2 ?m high. The microechinae density per sample area of 5 ?m x 5 ?m averages 17.4?2.4.

scholarly journals ELECTRON MICROSCOPIC STUDIES OF SOME ANTICANCER PLANTS POLLEN GRAINS

2021 ◽  
Vol 9 (10) ◽  
pp. 1386-1393
Author(s):  
Jayshree Sandesh Thaware ◽  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Pollen Morphology ◽  
Pollen Grains ◽  
Morphological Characters ◽  
Electron Microscopic ◽  
Plant Parts ◽  
Morphological Studies ◽  
Datura Metel ◽  
Scanning Electron

Pollen is appropriately referred by some as Golden dust extremely valuable on account of their tremendous applications in science, industries and public health. No other plant part even though extremely tiny in size is packed with so much information and power. Similar to other plant parts, pollen characters are so varied that the classification system of plants can be built up entirely on the basis of pollen morphology.Palynology is the distinct branch of biology that deals with the dispersed microscopic tiny living and fossil entities including pollen grains, spores, algal and fungal fragments and others. An important aspect of Palynology is the Pollen morphology. The importance of Palynology in taxonomic and phylogenetic consideration of plants is well known. The changes occurring through hybridization and years of cultivation are reflected in pollen morphology. The scope and interest in the study of pollen morphology have widened with the advent of Scanning Electron Microscopy (SEM) and with regards to unipalynous taxa particularly the understanding of finer morphology is of fundamental importance. SEM gives a correct understanding of exine surface as the electron photographs of the surface replica of the exine provides the exact picture of the ornamentation pattern. The variation in the pollen morphological characters helps in the classification of plant taxa and their assessment of their phylogenetic relationship. In the present investigation, the pollen morphological studies were carried out of some ethnomedicinal plants like Catharanthus roseus, Allamanda cathartica, Datura metel, Brassica juncea, Raphanus sativus and Cleome viscosa pollen grains by Scanning electron microscopy. All that they possess anticancer characteristics in common.

Pollen morphology of Sabinaria magnifica (Cryosophileae, Coryphoideae, Arecaceae)

Phytotaxa ◽  
2015 ◽  
Vol 207 (1) ◽  
pp. 135 ◽  
Author(s):  
Giovanni Raul Bogota ◽  
Carina Hoorn ◽  
Wim Star ◽  
Rob Langelaan ◽  
Hannah Banks ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Light Microscopy ◽  
Pollen Morphology ◽  
Pollen Grains ◽  
The Other ◽  
Transmission Electron ◽  
Palm Species ◽  
Scanning Electron

Sabinaria magnifica is so far the only known species in the recently discovered tropical palm genus Sabinaria (Arecaceae). Here we present a complete description of the pollen morphology of this palm species based on light microscopy (LM), scanning electron microscopy (SEM) and transmission electron microscopy (TEM). We also made SEM-based comparisons of Sabinaria with other genera within the tribe Cryosophileae. Pollen grains of Sabinaria magnifica resemble the other genera in the heteropolar, slightly asymmetric monads, and the monosulcate and tectate exine with perforate surface. Nevertheless, there are some clear differences with Thrinax, Chelyocarpus and Cryosophila in terms of aperture and exine. S. magnifica differs from its closest relative, Itaya amicorum, in the exine structure. This study shows that a combination of microscope techniques is essential for the identification of different genera within the Cryosophileae and may also be a necessary when working with other palynologically less distinct palm genera. 

Palynological analysis of five selected Onosma taxa

Biologia ◽  
2008 ◽  
Vol 63 (2) ◽  
Author(s):  
Filippo Maggi ◽  
Vladislav Kolarčik ◽  
Pavol Mártonfi
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Chromosome Number ◽  
Pollen Grains ◽  
Pollen Size ◽  
Palynological Analysis ◽  
Positive Correlation ◽  
Pollen Characters ◽  
Scanning Electron

AbstractPollen size and exine structure of the following five Onosma L. species from Slovakia and Italy were studied by light and scanning electron microscopy: Onosma echioides, O. tornensis, O. visianii, O. arenaria, and O. pseudoarenaria. Among the taxa studied, O. echioides has the smallest pollen grains in average (10.5–17.5 × 8.0–14.5 µm), and O. pseudoarenaria the largest grains in average (15.3–20.5 × 12.3–16.3 µm). Pollen grains of all 5 species are small sized, 3-syncolporate, subprolate, heteropolar, with ovate equatorial outlines and circular to rounded triangular polar outlines; the tectum is microechinate. A positive correlation is found between pollen size and chromosome number. The value of pollen characters for taxonomic purposes and the position of the taxa studied within the genus Onosma are discussed.

Sign in / Sign up

Export Citation Format

Share Document