Stagonosporopsis rhizophilae sp. nov. (Didymellaceae, Pleosporales), a new rhizospheric soil fungus associated with Populus deltoides Marsh

Phytotaxa ◽  
2021 ◽  
Vol 491 (1) ◽  
pp. 23-34
Author(s):  
HUANSHEN WEI ◽  
XINGHUA HE ◽  
BARONCELLI RICCARDO ◽  
YUZHAN YANG ◽  
ZHILIN YUAN
Keyword(s):  
Rna Polymerase Ii ◽  
Populus Deltoides ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Soil Fungus ◽  
Morphological Characters ◽  
Internal Transcribed Spacers ◽  
Rhizospheric Soil ◽  
Closely Related Species ◽  
A New Species

In this work we describe Stagonosporopsis rhizophilae sp. nov. (Didymellaceae, Pleosporales, Ascomycota), a new species isolated from the rhizospheric soil of Populus deltoides Marsh. Phylogenetic analyses based on combined multilocus sequences of internal transcribed spacers (ITS: ITS1, 5.8S, ITS2), and partial sequences of the large subunit 28S nrDNA region (LSU), β-tubulin (TUB), and RNA Polymerase II second-largest subunit (RPB2) clearly place this species within the genus Stagonosporopsis but distinguishes it from all known and closely related species such as S. nemophilae. Stagonosporopsis rhizophilae sp. nov. can also be differentiated from S. nemophilae by morphological characters and by the negative response of the NaOH spot test for the production of metabolite E.

scholarly journals Clitopilus lampangensis (Agaricales, Entolomataceae), a new species from northern Thailand

MycoKeys ◽  
2019 ◽  
Vol 58 ◽  
pp. 69-82
Author(s):  
Jaturong Kumla ◽  
Nakarin Suwannarach ◽  
Witchaphart Sungpalee ◽  
Kriangsak Sri-Ngernyuang ◽  
Saisamorn Lumyong
Keyword(s):  
New Species ◽  
Rna Polymerase Ii ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Internal Transcribed Spacers ◽  
Nuclear Ribosomal Dna ◽  
Full Description ◽  
Northern Thailand ◽  
Molecular Phylogenetic ◽  
A New Species

A new species of agaricomycetes, Clitopilus lampangensis, is described based on collections from northern Thailand. This species was distinguished from previously described Clitopilus species by its pale yellow to grayish yellow pileus with the presence of wider caulocystidia. Molecular phylogenetic analyses, based on the data of the internal transcribed spacers (ITS) and the large subunit (LSU) of the nuclear ribosomal DNA, and the second largest subunit of RNA polymerase II (rbp2) genes, also support the finding that C. lampangensis is distinct from other species within the genus Clitopilus. A full description, color photographs, illustrations and a phylogenetic tree showing the position of C. lampangensis are provided.

Morphological characters and phylogenetic analysis reveal a new species of Phellinus with hooked hymenial setae from Vietnam

Phytotaxa ◽  
2018 ◽  
Vol 356 (1) ◽  
pp. 91 ◽  
Author(s):  
LIN ZHU ◽  
XING JI ◽  
JING SI ◽  
BAO-KAI CUI
Keyword(s):  
Rna Polymerase Ii ◽  
Large Subunit ◽  
Elongation Factor ◽  
Morphological Characters ◽  
Molecular Data ◽  
Translation Elongation ◽  
The Status ◽  
Large Subunit Rdna ◽  
Elongation Factor 1 ◽  
A New Species

Phellinus vietnamensis sp. nov. is described from Vietnam based on morphological characters and molecular data. Morphologically, it is characterized by perennial, pileate basidiomata, a dimitic hyphal system, hooked hymenial setae, and colorless, broadly subglobose to ovoid, thick-walled basidiospores 5.5–6 × 4.8–5.2 μm. Phylogenetically, the status of Phellinus vietnamensis is strongly supported based on sequences of the nuclear internal transcribed spacer (ITS) regions, the translation elongation factor 1-α gene (EF1-α) nuclear large subunit rDNA (nrLSU) and the second largest subunits of RNA polymerase II (RPB2).

Morphological characters and molecular data reveal a new species of Rhodonia (Polyporales, Basidiomycota) from China

Phytotaxa ◽  
2017 ◽  
Vol 328 (2) ◽  
pp. 175
Author(s):  
YUAN YUAN ◽  
LU-LU SHEN
Keyword(s):  
New Species ◽  
Rna Polymerase Ii ◽  
Dna Sequences ◽  
Large Subunit ◽  
Morphological Characteristics ◽  
Northwest China ◽  
Morphological Characters ◽  
Molecular Data ◽  
Molecular Evidence ◽  
A New Species

A new polypore, Rhodonia tianshanensis, collected from West Tianshan Nature Reserve in Xinjiang Autonomous Region (northwest China), is described and illustrated based on morphological characteristics and molecular evidence. It is characterized by having resupinate basidiomata with an oblique tube layer, fusoid cystidioles in the hymenium, and cylindrical basidiospores. Based on multiple loci DNA sequences including the internal transcribed spacer (ITS), the large subunit (nLSU), and the second subunit of RNA polymerase II (RPB2) regions, our phylogeny strongly supported R. tianshanensis as a new species belonging to the genus Rhodonia.

scholarly journals A multi-gene phylogeny of Chlorophyllum (Agaricaceae, Basidiomycota): new species, new combination and infrageneric classification

MycoKeys ◽  
2018 ◽  
Vol 32 ◽  
pp. 65-90 ◽  
Author(s):  
Zai-Wei Ge ◽  
Adriaana Jacobs ◽  
Else C. Vellinga ◽  
Phongeun Sysouphanthong ◽  
Retha van der Walt ◽  
...  
Keyword(s):  
New Species ◽  
Rna Polymerase Ii ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Elongation Factor ◽  
Morphological Characters ◽  
Nuclear Ribosomal Dna ◽  
New Combination ◽  
Infrageneric Classification ◽  
Molecular Phylogenetic

Taxonomic and phylogenetic studies of Chlorophyllum were carried out on the basis of morphological differences and molecular phylogenetic analyses. Based on the phylogeny inferred from the internal transcribed spacer (ITS), the partial large subunit nuclear ribosomal DNA (nrLSU), the second largest subunit of RNA polymerase II (rpb2) and translation elongation factor 1-α (tef1) sequences, six well-supported clades and 17 phylogenetic species are recognised. Within this phylogenetic framework and considering the diagnostic morphological characters, two new species, C.africanum and C.palaeotropicum, are described. In addition, a new infrageneric classification of Chlorophyllum is proposed, in which the genus is divided into six sections. One new combination is also made. This study provides a robust basis for a more detailed investigation of diversity and biogeography of Chlorophyllum.

scholarly journals Morphological and Phylogenetic Reevaluation of the Genera Mycobonia and Pseudofavolus (Polyporaceae)

2021 ◽  
Author(s):  
Melissa Palacio ◽  
Mauro Westphalen ◽  
Yuan Yuan ◽  
Yingda Wu ◽  
Rosa Mara Borges Da Silveira
Keyword(s):  
Rna Polymerase Ii ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Internal Transcribed Spacers ◽  
Lsu Rdna ◽  
Evolutionary Analysis ◽  
Ancestral State ◽  
Tropical Asia ◽  
Genes Encoding ◽  
Ancestral States

Abstract Mycobonia and Pseudofavolus (Polyporales, Basidiomycota) are polyporoid genera with tropical and subtropical distribution. Both genera are morphologically similar in presenting flabelliform to conchate subsessile basidiomata, with a dimitic hyphal system, consisting of clamped generative hyphae and skeleto-binding hyphae that produce large basidiospores with smooth, thin walls. However, while Pseudofavolus species present a poroid hymenophore, in Mycobonia it is stereoid with hyphal pegs that resemble thin teeth. Mycobonia and Pseudofavolus have a controversial taxonomy, and the phylogenetic relationships between their species have yet to be assessed. For this reason, we performed molecular phylogenetic analyses on specimens of Mycobonia and Pseudofavolus from both the Neotropics and Asia, using internal transcribed spacers (ITS), the large subunit of nuclear rDNA (nc LSU rDNA), and also the genes encoding the second largest subunit of RNA polymerase II (RPB2). Furthermore, in order to develop an evolutionary analysis of the hymenophore configuration, we performed stochastic character mapping of ancestral states for the hymenophore type presented in Polyporus s.l. Our study revealed that Pseudofavolus is an artificial group and its species actually nest in a clade within Mycobonia. Therefore, in order to establish a monophyletic group, based upon priority of publication, we re-circunscribed Mycobonia to encompass both stereoid and poroid hymenophore species. Two new combinations are presented from the Neotropics: Mycobonia cucullata and M. miquelii. A new species from tropical Asia, M. yuchengii, is also described. We presente a summary of stochastic mapping of ancestral states estimates of hymenophore type in Polyporus s.l. The ancestral state for Mycobonia clade is estimated to have angular pores.

A new species and a new record of Clitopilus and a description of C. orientalis from India based on morphology and molecular phylogeny

Phytotaxa ◽  
2018 ◽  
Vol 343 (1) ◽  
pp. 47 ◽  
Author(s):  
K. N. ANIL RAJ ◽  
PATINJAREVEETTIL MANIMOHAN
Keyword(s):  
New Species ◽  
Maximum Likelihood ◽  
Molecular Phylogeny ◽  
Rna Polymerase Ii ◽  
Internal Transcribed Spacer ◽  
New Record ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Kerala State ◽  
A New Species

Three species of Clitopilus, C. albidus sp. nov., C. subscyphoides and C. orientalis, are described from Kerala State, India, based on morphology and molecular phylogeny. Comprehensive descriptions, photographs, and comparisons with phenetically similar and phylogenetically related species are provided. The RNA polymerase II genes (rpb2) of the three species were sequenced and analyzed. In addition, the nuclear ribosomal internal transcribed spacer regions (ITS) and the nuclear ribosomal large subunit (nLSU) genes were sequenced and used in BLAST searches to find similar sequences. Phylogenetic analyses based on rpb2 sequences performed using both Maximum Likelihood (ML) and Bayesian Analysis (BA) methods supported the novelty of C. albidus and its placement within the genus Clitopilus. C. subscyphoides and C. orientalis are the first and second record respectively of these species from India.

Pleurotus sirindhorniae (Pleurotaceae, Agaricales), a new species from northern Thailand

Phytotaxa ◽  
2020 ◽  
Vol 460 (4) ◽  
pp. 285-295
Author(s):  
NAKARIN SUWANNARACH ◽  
JATURONG KUMLA ◽  
KANITTA SATIENPERAKUL ◽  
WITCHAPHART SUNGPALEE ◽  
KRIANGSAK SRI-NGERNYUANG ◽  
...  
Keyword(s):  
New Species ◽  
Rna Polymerase Ii ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Elongation Factor ◽  
Morphological Characteristics ◽  
Internal Transcribed Spacers ◽  
Nuclear Ribosomal Dna ◽  
Full Description ◽  
Northern Thailand

A new saprophytic fungus, Pleurotus sirindhorniae, is described based on collections from northern Thailand. This species is characterized by greyish yellow to olive brown pileus, ellipsoid to elongate basidiospores and clavate to mucronate cheilocystidia. It is distinguished from the previously described Pleuotus species by its distinct morphological characteristics. Molecular phylogenetic analyses of the large subunit (LSU) and internal transcribed spacers (ITS) of the nuclear ribosomal DNA (rDNA), the translation elongation factor 1-alpha (tef1) and the RNA polymerase II largest subunit (rpb1) genes also support P. sirindhorniae as a distinct new species within the genus Pleurotus. A full description, color photographs, illustrations and a phylogenetic tree to show the position of P. sirindhorniae are provided.

Xanthagaricus thailandensis sp. nov. (Agaricales, Basidiomycota), from northern Thailand

Phytotaxa ◽  
2018 ◽  
Vol 348 (2) ◽  
pp. 109 ◽  
Author(s):  
JATURONG KUMLA ◽  
NAKARIN SUWANNARACH ◽  
KRIANGSAK SRI-NGERNYUANG ◽  
SAISAMORN LUMYONG
Keyword(s):  
New Species ◽  
Ribosomal Dna ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Internal Transcribed Spacers ◽  
Nuclear Ribosomal Dna ◽  
Full Description ◽  
Northern Thailand ◽  
Molecular Phylogenetic ◽  
A New Species

A new species of agaricomycetes, Xanthagaricus thailandensis, is described based on collections from northern Thailand. This species is characterized by its medium-sized basidiomata, pale orange pileus, a pseudoparenchymatous structure of the squamules on the pileal surface, subcylindrical to clavate caulocystidia and brownish yellow basidiospores. It is clearly distinguished from the previously described Xanthagaricus species by narrow caulocystidia. Molecular phylogenetic analyses, based on a combination of the internal transcribed spacers (ITS) and the large subunit (LSU) of the nuclear ribosomal DNA, also support the finding that X. thailandensis is distinct from other known species within the genus Xanthagaricus. A full description, color photographs, illustrations and a phylogenetic tree showing the position of X. thailandensis are provided.

scholarly journals Multilocus phylogenies reveal three new truffle-like taxa and the traces of interspecific hybridization in Octaviania (Boletaceae, Boletales)

IMA Fungus ◽  
2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Takamichi Orihara ◽  
Rosanne Healy ◽  
Adriana Corrales ◽  
Matthew E. Smith
Keyword(s):  
Rna Polymerase Ii ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Gene Tree ◽  
Phylogenetic Network ◽  
Elongation Factor ◽  
Morphological Observation ◽  
Tree Comparison ◽  
Unknown Species ◽  
The Usa

ABSTRACTAmong many convergently evolved sequestrate fungal genera in Boletaceae (Boletales, Basidiomycota), the genus Octaviania is the most diverse. We recently collected many specimens of Octaviania subg. Octaviania, including several undescribed taxa, from Japan and the Americas. Here we describe two new species in subgenus Octaviania, O. tenuipes and O. tomentosa, from temperate to subtropical evergreen Fagaceae forests in Japan based on morphological observation and robust multilocus phylogenetic analyses (nrDNA ITS and partial large subunit [LSU], translation elongation factor 1-α gene [TEF1] and the largest subunit of RNA polymerase II gene [RPB1]). Based on specimens from the Americas as well as studies of the holotype, we also taxonomically re-evaluate O. asterosperma var. potteri. Our analysis suggests that O. asterosperma var. potteri is a distinct taxon within the subgenus Octaviania so we recognize this as O. potteri stat. nov. We unexpectedly collected O. potteri specimens from geographically widespread sites in the USA, Japan and Colombia. This is the first verified report of Octaviania from the South American continent. Our molecular analyses also revealed that the RPB1 sequence of one O. tenuipes specimen was identical to that of a closely related species, O. japonimontana, and that one O. potteri specimen from Minnesota had an RPB1 sequence of an unknown species of O. subg. Octaviania. Additionally, one O. japonimontana specimen had an unusually divergent TEF1 sequence. Gene-tree comparison and phylogenetic network analysis of the multilocus dataset suggest that these heterogenous sequences are most likely the result of previous inter- and intra-specific hybridization. We hypothesize that frequent hybridization events in Octaviania may have promoted the high genetic and species diversity found within the genus.

Steinernema borjomiense n. sp. (Rhabditida: Steinernematidae), a new entomopathogenic nematode from Georgia

Nematology ◽  
2018 ◽  
Vol 20 (7) ◽  
pp. 653-669 ◽  
Author(s):  
Oleg Gorgadze ◽  
Elena Fanelli ◽  
Manana Lortkhipanidze ◽  
Alberto Troccoli ◽  
Medea Burjanadze ◽  
...  
Keyword(s):  
New Species ◽  
18S Rrna ◽  
Entomopathogenic Nematode ◽  
Phylogenetic Analyses ◽  
Tail Length ◽  
Morphological Characters ◽  
18S Rrna Gene ◽  
The Body ◽  
Rrna Gene ◽  
A New Species

Summary A new species of entomopathogenic nematode, Steinernema borjomiense n. sp., was isolated from the body of the host insect, Oryctes nasicornis (Coleoptera: Scarabaeidae), in Georgia, in the territory of Borjomi-Kharagauli. Morphological characters indicate that the new species is closely related to species of the feltiae-group. The infective juveniles are characterised by the following morphological characters: body length of 879 (777-989) μm, distance between the head and excretory pore = 72 (62-80) μm, pharynx length = 132 (122-142) μm, tail length = 70 (60-80) μm, ratio a = 26.3 (23.0-29.3), H% = 45 (40-51), D% = 54 (47-59), E% = 102 (95-115), and lateral fields consisting of seven ridges (eight incisures) at mid-body. Steinernema borjomiense n. sp. was molecularly characterised by sequencing three ribosomal regions (the ITS, the D2-D3 expansion domains and the 18S rRNA gene) and the mitochondrial COI gene. Phylogenetic analyses revealed that S. borjomiense n. sp. differs from all other known species of Steinernema and is a member of the monticolum-group.

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