scholarly journals Morphological and Phylogenetic Reevaluation of the Genera Mycobonia and Pseudofavolus (Polyporaceae)

2021 ◽  
Author(s):  
Melissa Palacio ◽  
Mauro Westphalen ◽  
Yuan Yuan ◽  
Yingda Wu ◽  
Rosa Mara Borges Da Silveira
Keyword(s):  
Rna Polymerase Ii ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Internal Transcribed Spacers ◽  
Lsu Rdna ◽  
Evolutionary Analysis ◽  
Ancestral State ◽  
Tropical Asia ◽  
Genes Encoding ◽  
Ancestral States

Abstract Mycobonia and Pseudofavolus (Polyporales, Basidiomycota) are polyporoid genera with tropical and subtropical distribution. Both genera are morphologically similar in presenting flabelliform to conchate subsessile basidiomata, with a dimitic hyphal system, consisting of clamped generative hyphae and skeleto-binding hyphae that produce large basidiospores with smooth, thin walls. However, while Pseudofavolus species present a poroid hymenophore, in Mycobonia it is stereoid with hyphal pegs that resemble thin teeth. Mycobonia and Pseudofavolus have a controversial taxonomy, and the phylogenetic relationships between their species have yet to be assessed. For this reason, we performed molecular phylogenetic analyses on specimens of Mycobonia and Pseudofavolus from both the Neotropics and Asia, using internal transcribed spacers (ITS), the large subunit of nuclear rDNA (nc LSU rDNA), and also the genes encoding the second largest subunit of RNA polymerase II (RPB2). Furthermore, in order to develop an evolutionary analysis of the hymenophore configuration, we performed stochastic character mapping of ancestral states for the hymenophore type presented in Polyporus s.l. Our study revealed that Pseudofavolus is an artificial group and its species actually nest in a clade within Mycobonia. Therefore, in order to establish a monophyletic group, based upon priority of publication, we re-circunscribed Mycobonia to encompass both stereoid and poroid hymenophore species. Two new combinations are presented from the Neotropics: Mycobonia cucullata and M. miquelii. A new species from tropical Asia, M. yuchengii, is also described. We presente a summary of stochastic mapping of ancestral states estimates of hymenophore type in Polyporus s.l. The ancestral state for Mycobonia clade is estimated to have angular pores.

scholarly journals Clitopilus lampangensis (Agaricales, Entolomataceae), a new species from northern Thailand

MycoKeys ◽  
2019 ◽  
Vol 58 ◽  
pp. 69-82
Author(s):  
Jaturong Kumla ◽  
Nakarin Suwannarach ◽  
Witchaphart Sungpalee ◽  
Kriangsak Sri-Ngernyuang ◽  
Saisamorn Lumyong
Keyword(s):  
New Species ◽  
Rna Polymerase Ii ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Internal Transcribed Spacers ◽  
Nuclear Ribosomal Dna ◽  
Full Description ◽  
Northern Thailand ◽  
Molecular Phylogenetic ◽  
A New Species

A new species of agaricomycetes, Clitopilus lampangensis, is described based on collections from northern Thailand. This species was distinguished from previously described Clitopilus species by its pale yellow to grayish yellow pileus with the presence of wider caulocystidia. Molecular phylogenetic analyses, based on the data of the internal transcribed spacers (ITS) and the large subunit (LSU) of the nuclear ribosomal DNA, and the second largest subunit of RNA polymerase II (rbp2) genes, also support the finding that C. lampangensis is distinct from other species within the genus Clitopilus. A full description, color photographs, illustrations and a phylogenetic tree showing the position of C. lampangensis are provided.

scholarly journals Integrative Studies on a New Ciliate Campanella sinica n. sp. (Protista, Ciliophora, Peritrichia) Based on the Morphological and Molecular Data, With Notes on the Phylogeny and Systematics of the Family Epistylididae

2021 ◽  
Vol 12 ◽  
Author(s):  
Zhe Wang ◽  
Tong Wu ◽  
Borong Lu ◽  
Yong Chi ◽  
Xue Zhang ◽  
...  
Keyword(s):  
Ribosomal Dna ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Small Subunit ◽  
Internal Transcribed Spacers ◽  
Lsu Rdna ◽  
5.8S Rdna ◽  
Freshwater Pond ◽  
The Family

During an investigation on freshwater peritrichs, a new colonial sessilid ciliate, Campanella sinica n. sp., was isolated from aquatic plants in an artificial freshwater pond in Qingdao, China. Specimen observations of this species were performed both in vivo and using silver staining. C. sinica n. sp. is characterized by the appearance of the mature colony, which is up to 2 cm high and contains more than 1,000 zooids, the asymmetric horn-shaped zooids, strongly everted and multi-layered peristomial lip, the slightly convex peristomial disc, and the well-developed haplokinety and polykinety, which make more than four circuits of the peristome before descending into the infundibulum. The small subunit ribosomal DNA (SSU rDNA), 5.8s rDNA and its flank internal transcribed spacers (ITS1-5.8s rDNA-ITS2), and large subunit ribosomal DNA (LSU rDNA) are sequenced and used for phylogenetic analyses which reveal that the family Epistylididae Kahl, 1933 is non-monophyletic whereas the genus Campanella is monophyletic and nests within the basal clade of the sessilids. The integrative results support the assertion that the genus Campanella represents a separate lineage from other epistylidids, suggesting a further revision of the family Epistylididae is needed. We revise Campanella including the transfer into this genus of a taxon formerly assigned to Epistylis, which we raise to species rank, i.e., Campanella ovata (Nenninger, 1948) n. grad. & n. comb. (original combination Epistylis purneri f. ovataNenninger, 1948). In addition, we provide a key to the identification of the species of Campanella.

Stagonosporopsis rhizophilae sp. nov. (Didymellaceae, Pleosporales), a new rhizospheric soil fungus associated with Populus deltoides Marsh

Phytotaxa ◽  
2021 ◽  
Vol 491 (1) ◽  
pp. 23-34
Author(s):  
HUANSHEN WEI ◽  
XINGHUA HE ◽  
BARONCELLI RICCARDO ◽  
YUZHAN YANG ◽  
ZHILIN YUAN
Keyword(s):  
Rna Polymerase Ii ◽  
Populus Deltoides ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Soil Fungus ◽  
Morphological Characters ◽  
Internal Transcribed Spacers ◽  
Rhizospheric Soil ◽  
Closely Related Species ◽  
A New Species

In this work we describe Stagonosporopsis rhizophilae sp. nov. (Didymellaceae, Pleosporales, Ascomycota), a new species isolated from the rhizospheric soil of Populus deltoides Marsh. Phylogenetic analyses based on combined multilocus sequences of internal transcribed spacers (ITS: ITS1, 5.8S, ITS2), and partial sequences of the large subunit 28S nrDNA region (LSU), β-tubulin (TUB), and RNA Polymerase II second-largest subunit (RPB2) clearly place this species within the genus Stagonosporopsis but distinguishes it from all known and closely related species such as S. nemophilae. Stagonosporopsis rhizophilae sp. nov. can also be differentiated from S. nemophilae by morphological characters and by the negative response of the NaOH spot test for the production of metabolite E.

Pleurotus sirindhorniae (Pleurotaceae, Agaricales), a new species from northern Thailand

Phytotaxa ◽  
2020 ◽  
Vol 460 (4) ◽  
pp. 285-295
Author(s):  
NAKARIN SUWANNARACH ◽  
JATURONG KUMLA ◽  
KANITTA SATIENPERAKUL ◽  
WITCHAPHART SUNGPALEE ◽  
KRIANGSAK SRI-NGERNYUANG ◽  
...  
Keyword(s):  
New Species ◽  
Rna Polymerase Ii ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Elongation Factor ◽  
Morphological Characteristics ◽  
Internal Transcribed Spacers ◽  
Nuclear Ribosomal Dna ◽  
Full Description ◽  
Northern Thailand

A new saprophytic fungus, Pleurotus sirindhorniae, is described based on collections from northern Thailand. This species is characterized by greyish yellow to olive brown pileus, ellipsoid to elongate basidiospores and clavate to mucronate cheilocystidia. It is distinguished from the previously described Pleuotus species by its distinct morphological characteristics. Molecular phylogenetic analyses of the large subunit (LSU) and internal transcribed spacers (ITS) of the nuclear ribosomal DNA (rDNA), the translation elongation factor 1-alpha (tef1) and the RNA polymerase II largest subunit (rpb1) genes also support P. sirindhorniae as a distinct new species within the genus Pleurotus. A full description, color photographs, illustrations and a phylogenetic tree to show the position of P. sirindhorniae are provided.

scholarly journals Multilocus phylogenies reveal three new truffle-like taxa and the traces of interspecific hybridization in Octaviania (Boletaceae, Boletales)

IMA Fungus ◽  
2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Takamichi Orihara ◽  
Rosanne Healy ◽  
Adriana Corrales ◽  
Matthew E. Smith
Keyword(s):  
Rna Polymerase Ii ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Gene Tree ◽  
Phylogenetic Network ◽  
Elongation Factor ◽  
Morphological Observation ◽  
Tree Comparison ◽  
Unknown Species ◽  
The Usa

ABSTRACTAmong many convergently evolved sequestrate fungal genera in Boletaceae (Boletales, Basidiomycota), the genus Octaviania is the most diverse. We recently collected many specimens of Octaviania subg. Octaviania, including several undescribed taxa, from Japan and the Americas. Here we describe two new species in subgenus Octaviania, O. tenuipes and O. tomentosa, from temperate to subtropical evergreen Fagaceae forests in Japan based on morphological observation and robust multilocus phylogenetic analyses (nrDNA ITS and partial large subunit [LSU], translation elongation factor 1-α gene [TEF1] and the largest subunit of RNA polymerase II gene [RPB1]). Based on specimens from the Americas as well as studies of the holotype, we also taxonomically re-evaluate O. asterosperma var. potteri. Our analysis suggests that O. asterosperma var. potteri is a distinct taxon within the subgenus Octaviania so we recognize this as O. potteri stat. nov. We unexpectedly collected O. potteri specimens from geographically widespread sites in the USA, Japan and Colombia. This is the first verified report of Octaviania from the South American continent. Our molecular analyses also revealed that the RPB1 sequence of one O. tenuipes specimen was identical to that of a closely related species, O. japonimontana, and that one O. potteri specimen from Minnesota had an RPB1 sequence of an unknown species of O. subg. Octaviania. Additionally, one O. japonimontana specimen had an unusually divergent TEF1 sequence. Gene-tree comparison and phylogenetic network analysis of the multilocus dataset suggest that these heterogenous sequences are most likely the result of previous inter- and intra-specific hybridization. We hypothesize that frequent hybridization events in Octaviania may have promoted the high genetic and species diversity found within the genus.

Spegazzinia camelliae sp. nov. (Didymosphaeriaceae, Pleosprales), a new endophytic fungus from northern Thailand

Phytotaxa ◽  
2021 ◽  
Vol 483 (2) ◽  
pp. 117-128
Author(s):  
NAKARIN SUWANNARACH ◽  
JATURONG KUMLA ◽  
SAISAMORN LUMYONG
Keyword(s):  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Elongation Factor ◽  
Small Subunit ◽  
Internal Transcribed Spacers ◽  
Nuclear Ribosomal Dna ◽  
Full Description ◽  
Translation Elongation ◽  
Spine Length ◽  
Elongation Factor 1 Alpha

A new endophytic ascomycete, described herein as Spegazzinia camelliae, was isolated from leaves of Camellia sinensis var. assamica collected from Nan Province, Thailand. This species is characterized by basauxic conidiophores and dark brown to blackish brown α and β conidia. It can be distinguished from previously described Spegazzinia species by the spine length of the α conidia and the size of the β conidia. Multi-gene phylogenetic analyses of the small subunit (SSU), large subunit (LSU) and internal transcribed spacers (ITS) of the nuclear ribosomal DNA (rDNA) and the translation elongation factor 1-alpha (tef1) genes also support S. camelliae is a distinct new species within Spegazzinia. A full description, color photographs, illustrations and a phylogenetic tree showing the position of S. camelliae are provided.

scholarly journals Sub1 Globally Regulates RNA Polymerase II C-Terminal Domain Phosphorylation

2010 ◽  
Vol 30 (21) ◽  
pp. 5180-5193 ◽  
Author(s):  
Alicia García ◽  
Emanuel Rosonina ◽  
James L. Manley ◽  
Olga Calvo
Keyword(s):  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Large Subunit ◽  
Terminal Domain ◽  
Mrna Metabolism ◽  
Genes Encoding ◽  
The One ◽  
Ctd Phosphorylation ◽  
Transcription Cycle

ABSTRACT The transcriptional coactivator Sub1 has been implicated in several aspects of mRNA metabolism in yeast, such as activation of transcription, termination, and 3′-end formation. Here, we present evidence that Sub1 plays a significant role in controlling phosphorylation of the RNA polymerase II large subunit C-terminal domain (CTD). We show that SUB1 genetically interacts with the genes encoding all four known CTD kinases, SRB10, KIN28, BUR1, and CTK1, suggesting that Sub1 acts to influence CTD phosphorylation at more than one step of the transcription cycle. To address this directly, we first used in vitro kinase assays, and we show that, on the one hand, SUB1 deletion increased CTD phosphorylation by Kin28, Bur1, and Ctk1 but, on the other, it decreased CTD phosphorylation by Srb10. Second, chromatin immunoprecipitation assays revealed that SUB1 deletion decreased Srb10 chromatin association on the inducible GAL1 gene but increased Kin28 and Ctk1 chromatin association on actively transcribed genes. Taken together, our data point to multiple roles for Sub1 in the regulation of CTD phosphorylation throughout the transcription cycle.

scholarly journals Multi-gene phylogeny and taxonomy of Amauroderma s. lat. (Ganodermataceae)

2020 ◽  
Vol 44 (1) ◽  
pp. 206-239 ◽  
Author(s):  
Y.-F. Sun ◽  
D.H. Costa-Rezende ◽  
J.-H. Xing ◽  
J.-L. Zhou ◽  
B. Zhang ◽  
...  
Keyword(s):  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Elongation Factor ◽  
Morphological Examination ◽  
Translation Elongation ◽  
Multiple Loci ◽  
Phylogenetic Studies ◽  
Molecular Phylogenetic

Amauroderma s.lat. has been defined mainly by the morphological features of non-truncate and double-walled basidiospores with a distinctly ornamented endospore wall. In this work, taxonomic and phylogenetic studies on species of Amauroderma s.lat. are carried out by morphological examination together with ultrastructural observations, and molecular phylogenetic analyses of multiple loci including the internal transcribed spacer regions (ITS), the large subunit of nuclear ribosomal RNA gene (nLSU), the largest subunit of RNA polymerase II (RPB1) and the second largest subunit of RNA polymerase II (RPB2), the translation elongation factor 1-α gene (TEF) and the β-tubulin gene (TUB). The results demonstrate that species of Ganodermataceae formed ten clades. Species previously placed in Amauroderma s.lat. are divided into four clades: Amauroderma s.str., Foraminispora, Furtadoa and a new genus Sanguinoderma. The classification of Amauroderma s. lat. is thus revised, six new species are described and illustrated, and eight new combinations are proposed. SEM micrographs of basidiospores of Foraminispora and Sanguinoderma are provided, and the importance of SEM in delimitation of taxa in this study is briefly discussed. Keys to species of Amauroderma s.str., Foraminispora, Furtadoa, and Sanguinoderma are also provided.

Coleopuccinia in China and its relationship to Gymnosporangium

Phytotaxa ◽  
2018 ◽  
Vol 347 (3) ◽  
pp. 235 ◽  
Author(s):  
BIN CAO ◽  
SIQI TAO ◽  
CHENGMING TIAN ◽  
YINGMEI LIANG
Keyword(s):  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Rust Fungi ◽  
Internal Transcribed Spacers ◽  
Western China ◽  
Rrna Gene ◽  
Morphological Examination ◽  
Separate Genus ◽  
Lsu Rrna Gene ◽  
Lsu Rrna

The rust species Coleopuccinia sinensis was collected during an investigation of rust fungi in Tibet, a region in south-western China. Through morphological examination, we clarified that C. kunmingensis is a synonym of C. sinensis. Phylogenetic analyses using the combined loci of the internal transcribed spacers (ITS) and the partial large subunit (LSU) rRNA gene revealed that Coleopuccinia should be treated as a separate genus from Gymnosporangium.

Molecular and morphological evidence indicates that Pseudorhabdosynochus lantauensis (Monogenea: Diplectanidae) represents two species

Parasitology ◽  
2005 ◽  
Vol 130 (6) ◽  
pp. 669-677 ◽  
Author(s):  
X. Y. WU ◽  
N. B. CHILTON ◽  
X. Q. ZHU ◽  
M. Q. XIE ◽  
A. X. LI
Keyword(s):  
Sequence Data ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Strong Support ◽  
Morphological Characteristics ◽  
Sufficient Evidence ◽  
Bootstrap Support ◽  
Morphological Evidence ◽  
Lsu Rdna ◽  
Epinephelus Coioides

Sequences of the first internal transcribed spacer (ITS-1) and the D1-D3 domains of the large subunit (LSU) of the ribosomal DNA (rDNA) were determined for multiple specimens of 4 operational taxonomic units (OTUs) of the monogenean, Pseudorhabdosynochus lantauensis. OTUs were defined based on their collecting localities, host and/or morphological characteristics. All P. lantauensis specimens of one group (OTUs 1 and 3) differed in their sequences of the ITS-1 and partial LSU rDNA when compared with specimens of a second group (OTUs 2 and 4) by 12% and 2%, respectively. Results of the phylogenetic analyses of the LSU rDNA sequence data showed total (100%) bootstrap support for the separation of P. lantauensis into 2 distinct clades. At least 11 of the 18 nucleotide differences in the LSU sequence between the two P. lantauensis clades were derived (i.e. autapomorphic) characters when the morphologically distinct species, P. epinepheli and P. coioidesis, were used as outgroups. Furthermore, there were several autapomorphic character states for each P. lantauensis clade. This provides sufficient evidence to reject the null hypothesis that P. lantauensis represents a single species. Morphological and morphometric differences between these two clades provided additional strong support for the separation of P. lantauensis into two species. These two parasite species were found to co-exist on one of the two species of serranid fish (i.e. Epinephelus coioides) examined in the South China Sea (Guangdong Province, China).

Sign in / Sign up

Export Citation Format

Share Document