Aspergillus sibiricus (Aspergillaceae, Eurotiales), a novel acid-tolerant species in Aspergillus section Fumigati

Phytotaxa ◽  
2022 ◽  
Vol 531 (1) ◽  
pp. 63-72

A novel species in Aspergillus section Fumigati is described as Aspergillus sibiricus. The new species was isolated from an open coal mine in Altai, Siberia, Russia and is represented by ex-type strain CBS 143307. We used a polyphasic taxonomic approach to characterise the species. A description based on morphological features is provided and shows that the new species differs morphologically from closely related species (A. assulatus, A. nishimurae and A. waksmanii). Partial sequences of the loci encoding β-tubulin (BenA), calmodulin (CaM), ribosomal polymerase II second largest subunit (RPB2) and internal transcribed spacer rDNA region (ITS1-5.8S-ITS2) were analyzed as well. Sequences data, macro- and micromorphological and physiological characteristics distinguish A. sibiricus from all known species in section Fumigati, series Unilaterales. A. sibiricus, isolated from an acidic habitat (pH 3.2–3.4), is able to grow at pH 2 and is considered an acid-resistant species.

Moritz K. Jansson ◽  
Silvio Hering ◽  
Michael E. J. Buhl

A strain of obligately anaerobically growing Gram-positive cocci was isolated from a human genito-urinary sample and characterized by a polyphasic approach. Analyses of 16S rRNA gene and whole-genome sequences of this strain S3374T indicated that it belonged to the genus Parvimonas . Overall genome relatedness index calculations confirmed it to be phylogenetically distinct from Parvimonas micra (NCTC 11808T) as its most closely related species with standing in nomenclature, with average nucleotide identity and genome-to-genome distance values of 85.8 and 30.2 %, respectively. Biochemically, strain S3374T was strongly proteolytic and can be differentiated from P. micra (DSM 20468T) by absence of phosphatase activity. The DNA G+C content of strain S3374T was 28.6 mol%. Based on the phenotypical, biochemical and genetic findings, strain S3374T is considered to represent a novel species within the genus Parvimonas , for which the name Parvimonas parva sp. nov. is proposed. The type strain is S3374T (=DSM 110786T=CCOS 1934T=CCUG 74294T). This description adds strain S3374T as a second species to the genus Parvimonas which has so far been monotypic. While the type strain of this genus, P. micra , has a long standing in nomenclature and its role in human health and disease has been studied to some extent, this description of the proposed novel species represented by strain S3374T will allow microbiologists worldwide to identify isolates of P. parva sp. nov., a prerequisite for further investigation of its relevance in the clinical context and beyond.

2007 ◽  
Vol 57 (11) ◽  
pp. 2562-2567 ◽  
Geok Yuan Annie Tan ◽  
Stuart Robinson ◽  
Ernest Lacey ◽  
Roselyn Brown ◽  
Wonyong Kim ◽  

The taxonomic position of seven soil actinomycetes provisionally assigned to the genus Amycolatopsis was established in a polyphasic study. The isolates, which had identical 16S rRNA gene sequences, had closest similarity to the type strain of Amycolatopsis orientalis. A representative isolate, strain GY080T, had chemotaxonomic properties that were typical of the genus Amycolatopsis and could be distinguished from the type strain of A. orientalis using DNA–DNA relatedness data. All of the isolates shared a phenotypic profile that distinguished them from representatives of phylogenetically closely related species. Amplified rDNA restriction analysis showed that the isolates formed a homogeneous group that was distinctly separate from single-membered groups consisting of representative Amycolatopsis type strains, including that of A. orientalis. Based on the combined genotypic and phenotypic evidence, it is proposed that the seven isolates be classified as representatives of a novel species for which the name Amycolatopsis regifaucium sp. nov. is proposed. The type strain is GY080T (=DSM 45072T =NCIMB 14277T).

2010 ◽  
Vol 60 (6) ◽  
pp. 1460-1465 ◽  
Dennis S. Nielsen ◽  
Mogens Jakobsen ◽  
Lene Jespersen

During an investigation of the microbiology of Ghanaian cocoa fermentations, a number of yeast isolates with unusual pheno- and genotypic properties representing three possible novel species were isolated. Members of Group A divided by multilateral budding and ascospores were not produced. Group B strains produced true hyphae and ascospores were not produced. Group C representatives divided by budding and formed chains and star-like aggregates. Ascospores were not produced. Sequence analysis of the 26S rRNA gene (D1/D2 region) revealed that the Group A isolates were phylogenetically most closely related to Saturnispora mendoncae (gene sequence similarity 92.4 %), Saturnispora besseyi (88.8 %), Saturnispora saitoi (88.8 %) and Saturnispora ahearnii (88.3 %). Members of Group B were most closely related to representatives of the genera Dipodascus and Galactomyces and the asporogenous genus Geotrichum, but in all cases with 26S rRNA gene (D1/D2 region) similarities below 87 %. For Group C, the most closely related species were Candida rugopelliculosa (92.4 %), Pichia occidentalis (91.6 %) and Pichia exigua (91.9 %). The very low gene sequence similarities obtained for the three groups of isolates clearly indicated that they represented novel species. Repetitive Palindromic PCR (Rep-PCR) of the isolates and their closest phylogenetic relatives confirmed that the new isolates belonged to previously undescribed species. In conclusion, based on the genetic and phenotypic results, the new isolates were considered to represent three novel species, for which the names Candida halmiae (group A, type strain G3T=CBS 11009T=CCUG 56721T); Geotrichum ghanense (group B, type strain G6T=CBS 11010T=CCUG 56722T) and Candida awuaii (group C, type strain G15T=CBS 11011T=CCUG 56723T) are proposed.

2010 ◽  
Vol 60 (12) ◽  
pp. 2882-2886 ◽  
Jun Zhang ◽  
Rui-Bo Jiang ◽  
Xiao-Xia Zhang ◽  
Bao-Jian Hang ◽  
Jian He ◽  

A Gram-negative, non-spore-forming, yellow-pigmented bacterium, strain LQY-7T, was isolated from activated sludge treating synthetic pyrethroid-manufacturing wastewater. The taxonomic status of the strain was determined using a polyphasic taxonomic approach. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain LQY-7T was a member of the genus Flavobacterium but had low similarities with other species of this genus (95.0 % similarity with Flavobacterium indicum GPTSA100-9T and <94 % similarities with other Flavobacterium species). On the basis of phenotypic, genetic and phylogenetic data, strain LQY-7T should be classified as a representative of a novel species of the genus Flavobacterium, for which the name Flavobacterium haoranii sp. nov. is proposed; the type strain is LQY-7T (=ACCC 05409T =KCTC 23008T).

Yang Wang ◽  
Kundi Zhang ◽  
Feng Cai ◽  
Lei Zhang ◽  
Yali Tang ◽  

A novel strain, designated 311-10T, isolated from soil of Xinjiang, China, was characterized by using a polyphasic taxonomic approach. The isolate was Gram-negative, aerobic, rod-shaped, non-motile, oxidase-negative and catalase-positive. The predominant menaquinone of strain 311-10T was MK-7 and the genomic DNA G+C content was 47.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolate formed a cluster with the genera Pontibacter and [Effluviibacter] in the phylum ‘Bacteroidetes’, with sequence similarities of 93.9–95.6 %. Phylogenetic evidence and the results of phenotypic, genotypic and chemotaxonomic analyses support the reclassification of [Effluviibacter] roseus as Pontibacter roseus comb. nov. (type strain, SRC-1T=MTCC 7260T=DSM 17521T) and the establishment of a novel species, Pontibacter xinjiangensis sp. nov., with strain 311-10T (=CCTCC AB 207200T=NRRL B-51335T) as the type strain.

2004 ◽  
Vol 54 (1) ◽  
pp. 191-194 ◽  
James E. M. Stach ◽  
Luis A. Maldonado ◽  
Alan C. Ward ◽  
Alan T. Bull ◽  
Michael Goodfellow

The taxonomic position of a marine actinomycete, strain SJS0289/JS1T, was determined using a polyphasic taxonomic approach. The organism, which had phenotypic properties consistent with its classification in the genus Williamsia, formed a distinct clade in the 16S rRNA gene tree together with the type strain of Williamsia muralis, but was readily distinguished from this species using DNA–DNA relatedness and phenotypic data. The genotypic and phenotypic data show that the organism merits recognition as a new species of Williamsia. The name proposed for the new species is Williamsia maris; the type strain is SJS0289/JS1T (=DSM 44693T=JCM 12070T=KCTC 9945T=NCIMB 13945T).

2010 ◽  
Vol 60 (10) ◽  
pp. 2501-2506 ◽  
Wladyslav I. Golubev ◽  
Gloria Scorzetti

Three novel species are described as Rhodotorula rosulata sp. nov. (type strain VKM Y-2962T =CBS 10977T), Rhodotorula silvestris sp. nov. (type strain VKM Y-2971T =CBS 11420T) and Rhodotorula straminea sp. nov. (type strain VKM Y-2964T =CBS 10976T) based on the study of eight isolates from needle litter. The new species, phylogenetically located within the Microbotryomycetes, are related to glucuronate-assimilating species of the genus Rhodotorula. Sequencing of the D1/D2 domains of the LSU rDNA gene and the internal transcribed spacer (ITS) region, as well as physiological characterization, revealed their distinct taxonomic positions.

2011 ◽  
Vol 61 (7) ◽  
pp. 1554-1560 ◽  
Yang Wang ◽  
Feng Cai ◽  
Yali Tang ◽  
Jun Dai ◽  
Huan Qi ◽  

A novel strain, designated HY-50RT, isolated from soil of a Euphrates poplar (Populus euphratica) forest in Xinjiang, China, was characterized using a polyphasic taxonomic approach. Cells of the isolate were Gram-reaction-negative, strictly aerobic, rod-shaped, non-motile, oxidase-negative and catalase-positive. Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolate was a member of the phylum Bacteroidetes, its closest relatives being Niastella populi THYL-44T (93.6 % similarity), Flavisolibacter ginsengisoli Gsoil 643T (93.5 %), Terrimonas ferruginea IAM 15098T (93.3 %) and Flavisolibacter ginsengiterrae Gsoil 492T (93.2 %). The major fatty acids were iso-C15 : 1 G (11.7 %), iso-C15 : 0 (19.6 %) and iso-C17 : 0 3-OH (19.3 %). The predominant menaquinone of strain HY-50RT was MK-7 and the genomic DNA G+C content was 46.8 mol%. Flexirubin-type pigments were not produced. Based on phylogenetic evidence and the results of phenotypic, genotypic and chemotaxonomic analysis, strain HY-50RT represents a novel species of a novel genus, for which the name Flavitalea populi gen. nov., sp. nov. is proposed. The type strain is HY-50RT ( = CCTCC AB 208255T  = NRRL B-59222T).

Phytotaxa ◽  
2016 ◽  
Vol 284 (1) ◽  
pp. 41 ◽  
LEI YE ◽  

The novel species Laccaria rubroalba is described from Southwestern China by using both morphological characteristics and molecular data. It is characterized by small basidiomata; reddish white pileus when moist or young, white to pale when dry; 4-spored basidia and globose to broadly ellipsoid, hyaline, moderately echinulate basidiospores. Phylogenetic relationships inferred from ITS sequence data confirmed the separation of this new species from other closely related species in the genus Laccaria. The new species is compared with similar taxa. A description, line drawings and colour photographs of the new species, and phylogenetic tree to show the placement of the new species are provided.

2020 ◽  
Vol 70 (10) ◽  
pp. 5363-5372 ◽  
Anne-Sophie Le Guern ◽  
Cyril Savin ◽  
Hilde Angermeier ◽  
Sylvie Brémont ◽  
Dominique Clermont ◽  

Thirty-three Yersinia strains previously characterized by the French Yersinia National Reference Laboratory (YNRL) and isolated from humans and animals were suspected to belong to six novel species by a recently described core genome multilocus sequence typing scheme. These strains and five additional strains from the YNRL were characterized using a polyphasic taxonomic approach including a phylogenetic analysis based on 500 core genes, determination of average nucleotide identity (ANI), determination of DNA G+C content and identification of phenotypic features. Phylogenetic analysis confirmed that the 38 studied strains formed six well-demarcated clades. ANI values between these clades and their closest relatives were <94.7 % and ANI values within each putative novel species were >97.5 %. Distinctive biochemical characteristics were identified in five out of the six novel species. All of these data demonstrated that the 38 strains belong to six novel species of the genus Yersinia : Yersinia artesiana sp. nov., type strain IP42281T (=CIP 111845T=DSM 110725T); Yersinia proxima sp. nov., type strain IP37424T (=CIP 111847T=DSM 110727T); Yersinia alsatica sp. nov., type strain IP38850T (=CIP 111848T=DSM 110726T); Yersinia vastinensis sp. nov., type strain IP38594T (=CIP 111844T=DSM 110738T); Yersinia thracica sp. nov., type strain IP34646T (=CIP 111842T=DSM 110736T); and Yersinia occitanica sp. nov., type strain IP35638T (=CIP 111843T=DSM 110739T).

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