Morphological and molecular evidence for a new species of longnose skate (Rajiformes: Rajidae: Dipturus) from Argentinean waters based on DNA barcoding

Zootaxa ◽  
2008 ◽  
Vol 1921 (1) ◽  
pp. 35-46 ◽  
Author(s):  
JUAN MARTIN DÍAZ DE ASTARLOA ◽  
EZEQUIEL MABRAGAÑA ◽  
ROBERT HANNER ◽  
DANIEL E. FIGUEROA
Keyword(s):  
New Species ◽  
Sequence Data ◽  
Sequence Divergence ◽  
Color Pattern ◽  
Molecular Evidence ◽  
Dna Barcodes ◽  
Southwestern Atlantic ◽  
Cytochrome C Oxidase I ◽  
Molecular Approaches ◽  
A New Species

A new species of Dipturus is described from ten specimens collected off Patagonia, Argentina. Morphological and molecular approaches were used to compare among specimens of recognized Dipturus species. By comparing morphometric, meristic and mitochondrial cytochrome c oxidase I (COI) sequence data, specimens referred to as longnose skate and originally regarded as D. chilensis were shown to be a discrete species as distinguished from both the Yellownose skate, D. chilensis and the Roughskin skate, D. trachyderma. Dipturus argentinensis n. sp. can be distinguished from all other southwestern Atlantic longnose skate species by its color pattern, lack of squamation on both upper and lower surfaces of the disc, and a long, thin tail that is approximately half the total length. The new species has one median row of 10 to 24 small caudal thorns, one or two interdorsal thorns and 35 to 40, and 34 to 43 tooth rows on upper and lower jaws, respectively. The 648 base pair COI mitochondrial DNA “barcodes” derived from specimens of D. argentinensis are identical to each other and exhibit greater than 3% sequence divergence from all other Dipturus species similarly characterized to date. Taken together, these independent morphological and molecular observations serve to corroborate one another and thus provide strong evidence for the recognition of D. argentinensis as a new species.

A new species of jumping pitviper of the genus Atropoides (Serpentes: Viperidae: Crotalinae) from the Sierra de Botaderos and the Sierra La Muralla, Honduras

Zootaxa ◽  
2008 ◽  
Vol 1948 (1) ◽  
pp. 57-68 ◽  
Author(s):  
ERIC N. SMITH ◽  
JORGE A. FERRARI-CASTRO
Keyword(s):  
New Species ◽  
Gene Sequence ◽  
Sequence Data ◽  
Mitochondrial Gene ◽  
Sequence Divergence ◽  
Morphological Characters ◽  
The Body ◽  
Molecular Evidence ◽  
A New Species ◽  
Gene Sequence Data

We describe a new species of pitviper of the genus Atropoides from the Sierra de Botaderos and La Muralla, Honduras. The new species is easily distinguished from all other members of the genus, except A. picadoi from Costa Rica and Panama, by possessing more ventral scales (140 vs. 103–138). This new species differs from A. picadoi in being relatively small, less than 600 mm in total length (vs. reaching > 750 mm), having the posterior third of the body venter heavily melanized (vs. more than 50%), less than 50% of the underside of the tail melanized, and the postorbital stripe covering more than 50% of only one or two scales from the first temporal row and covering completely only the last scale of the row, at most (vs. 3–4 more than 50% melanized and the last two scales in the row usually completely melanized). In addition to morphological characters, molecular evidence also differentiates this new species from the other species of Atropoides (as recognized by Castoe et al. 2005). Using mitochondrial gene sequence data, they found the new species described herein to represent the sister species of A. occiduus, with 5.7 % sequence divergence separating these two taxa.

Additions to the decapod crustacean fauna of the Hawaiian Islands, III. A new species of the genus Babamunida (Crustacea: Galatheidae) from Hawaii based on morphological and molecular evidence

Zootaxa ◽  
2009 ◽  
Vol 2130 (1) ◽  
pp. 21-30 ◽  
Author(s):  
KAREEN E. SCHNABEL ◽  
JOEL W. MARTIN ◽  
ROBERT B. MOFFITT
Keyword(s):  
New Species ◽  
Sequence Data ◽  
Hawaiian Islands ◽  
Decapod Crustacean ◽  
Color Pattern ◽  
Molecular Evidence ◽  
Dna Sequence Data ◽  
Ecological Information ◽  
Northwestern Hawaiian Islands ◽  
A New Species

Babamunida kanaloa n. sp., a new galatheid from approximately 250 m depth off the Northwestern Hawaiian Islands (French Frigate Shoals), is described. DNA sequence data from two gene regions (16S and CO1) corroborates the placement of this new species in the genus Babamunida. Images taken independently using the Deep Submergence Vehicle Pisces IV are almost certainly of the same species based on overall size and color pattern and provide limited ecological information. This new species brings the number of species in the genus Babamunida to six and the total number of galatheid species known from the Hawaiian Islands to four (possibly higher depending on some previous records that might be questionable).

A new species of Bent-toed Gecko (Squamata, Gekkonidae, Cyrtodactylus) from the Shan Plateau in eastern Myanmar (Burma)

Zootaxa ◽  
2019 ◽  
Vol 4624 (3) ◽  
pp. 301-321 ◽  
Author(s):  
L. LEE GRISMER ◽  
PERRY L. JR. WOOD ◽  
EVAN S. H. QUAH ◽  
MYINT KYAW THURA ◽  
JAMIE R. OAKS ◽  
...  
Keyword(s):  
New Species ◽  
Hot Spot ◽  
Mitochondrial Gene ◽  
Sequence Divergence ◽  
Morphological Characters ◽  
Color Pattern ◽  
Pairwise Sequence Divergence ◽  
Taxonomic Analysis ◽  
A New Species ◽  
Karst Habitats

An integrative taxonomic analysis of the Cyrtodactylus linnwayensis group of the Shan Plateau recovered two new populations from isolated karst habitats near Pinlaung Town, Shan State as a new species, C. pinlaungensis sp. nov. Cyrtodactylus pinlaungensis sp. nov. is most closely related to a clade comprising C. linnwayensis and C. ywanganensis from the western edge of the Shan Plateau approximately 90 km to the northwest. Cyrtodactylus pinlaungensis sp. nov. can be distinguished from all members of the C. linnwayensis group by a number of statistically different morphological characters, discrete color pattern differences, and its heavy tuberculation. It also bears an uncorrected pairwise sequence divergence of 5.0–7.6% from all other species combined based on the mitochondrial gene ND2 and its flanking tRNAs. The discovery of this new species on the Shan Plateau continues to underscore the fact that this region is rapidly emerging as a herpetological diversity hot-spot for Myanmar. 

A new species of big-headed, fanged dicroglossine frog (Genus Limnonectes) from Thailand

Zootaxa ◽  
2008 ◽  
Vol 1807 (1) ◽  
pp. 26 ◽  
Author(s):  
DAVID S. McLEOD
Keyword(s):  
New Species ◽  
Dna Sequence ◽  
Sequence Data ◽  
Sequence Divergence ◽  
Type Material ◽  
Base Pairs ◽  
Dna Sequence Data ◽  
16S Gene ◽  
A New Species

A new species of the dicroglossine genus Limnonectes from eastern Thailand and its tadpole are described. Analysis of DNA sequence data from 2518 base-pairs of the mitochondrial 12S and 16S gene regions places the species within the complex of frogs currently referred to as Limnonectes kuhlii and demonstrates it to be a separate lineage (>18% sequence divergence from type-material of L. kuhlii from Java). The new species differs from L. kuhlii by having nuptial pads, a greater snout–vent length, and different relative finger lengths than specimens from Java. It has more extensive toe webbing, a different arrangement of nuptial pads, and a greater snout–vent length than Limnonectes laticeps. The new species, which lacks vocal slits, also can be distinguished from the morphologically similar Limnonectes namiyei from Japan, which possesses vocal slits.

Pilea nonggangensis (Urticaceae), a new species from Guangxi, China

Phytotaxa ◽  
2017 ◽  
Vol 313 (1) ◽  
pp. 130 ◽  
Author(s):  
LONG-FEI FU ◽  
SHI-LIAN HUANG ◽  
ALEX K. MONRO ◽  
YING LIU ◽  
FANG WEN ◽  
...  
Keyword(s):  
New Species ◽  
Internal Transcribed Spacer ◽  
Sequence Data ◽  
Species Conservation ◽  
Its Region ◽  
Systematic Position ◽  
Molecular Evidence ◽  
Conservation Assessment ◽  
Dna Sequence Data ◽  
A New Species

Pilea nonggangensis Y. G. Wei, L. F. Fu & A. K. Monro, a new species from Guangxi, China is described and illustrated. The new species is morphologically most similar to P. basicordata from which it differs by being dioecious, having peltate leaf blades, membranous stipules, paired inflorescences and larger bracts. Nuclear ribosomal internal transcribed spacer (ITS) region and plastid trnL-F intron spacer (trnL-F) DNA sequence data from the new species and 20 local congeneric species are used to resolve the systematic position of the new species within Pilea. Despite vastly different morphology the molecular evidence suggests that P. nonggangensis is most closely related to P. pseudonotata. A Global Species Conservation Assessment classifies P. nonggangensis as Vulnerable (VU).

scholarly journals Two new species and a new species record of Aglaia (Meliaceae) from Indonesia

PhytoKeys ◽  
2020 ◽  
Vol 155 ◽  
pp. 33-51
Author(s):  
Caroline M. Pannell ◽  
Jan Schnitzler ◽  
Alexandra N. Muellner-Riehl
Keyword(s):  
New Species ◽  
Sequence Data ◽  
Geographic Range ◽  
Dna Barcodes ◽  
Dichotomous Key ◽  
West Papua ◽  
Two New Species ◽  
Nuclear Its ◽  
Sabah And Sarawak ◽  
A New Species

Two new species of Aglaia from Indonesia are described, Aglaia monocaula restricted to West Papua, and Aglaia nyaruensis occurring on Borneo (Kalimantan, Brunei, Sabah and Sarawak). A phylogenetic analysis using nuclear ITS and ETS, and plastid rps15-ycf1 sequence data indicates that the two new species of Aglaia are also genetically distinct. Aglaia monocaula belongs to sectionAmoora, while A. nyaruensis is included in section Aglaia. A dichotomous key, drawings and three-locus DNA barcodes are provided as aids for the identification of the two new species of Aglaia. In addition, the geographic range of Aglaia mackiana (section Amoora) is expanded from a single previously known site in Papua New Guinea to West Papua, Indonesia.

"Same-same, but different": an unusual new species of the Limnonectes kuhlii Complex from West Sumatra (Anura: Dicroglossidae)

Zootaxa ◽  
2011 ◽  
Vol 2883 (1) ◽  
pp. 52 ◽  
Author(s):  
DAVID S. MCLEOD ◽  
STEPHANI J. HORNER ◽  
COLIN HUSTED ◽  
ANTHONY BARLEY ◽  
DJOKO ISKANDAR
Keyword(s):  
New Species ◽  
Sequence Data ◽  
Sequence Divergence ◽  
Sensu Stricto ◽  
Morphological Data ◽  
Sister Taxon ◽  
Dna Sequence Data ◽  
16S Gene ◽  
West Sumatra ◽  
A New Species

A new species in the dicroglossine genus Limnonectes from West Sumatra, Indonesia, is described. Analysis of DNA sequence data from the mitochondrial 12S and 16S gene regions places the species within the Limnonectes kuhlii Complex and demonstrates it to be the sister taxon of Limnonectes kuhlii sensu stricto from Java. Both molecular and morphological data support the recognition of this lineage as a new species. Notably, the presence of a spinule-covered mental plate distinguishes Limnonectes sisikdagu sp. nov. from all other members of the L. kuhlii Complex. Additionally, pair-wise sequence divergence greater than 10% separate the new species from its sister taxon, L. kuhlii from Java.

Another new Cyrtodactylus (Squamata: Gekkonidae) from Guwahati, Assam, India

Zootaxa ◽  
2020 ◽  
Vol 4732 (3) ◽  
pp. 375-392
Author(s):  
JAYADITYA PURKAYASTHA ◽  
MADHURIMA DAS ◽  
SANATH CHANDRA BOHRA ◽  
AARON M. BAUER ◽  
ISHAN AGARWAL
Keyword(s):  
New Species ◽  
Additional Data ◽  
Sequence Data ◽  
Sequence Divergence ◽  
Northeast India ◽  
The State ◽  
Colour Pattern ◽  
Mitochondrial Sequence ◽  
Guwahati City ◽  
A New Species

We describe a new species of Cyrtodactylus from Guwahati city in the state of Assam, India and provide additional data on the recently described Cyrtodactylus guwahatiensis. Cyrtodactylus urbanus sp. nov. falls in the newly defined khasiensis group within the Indo-Burma clade of Cyrtodactylus and is the poorly supported sister taxon to Cyrtodactylus khasiensis. The new species differs from other members of the khasiensis group in mitochondrial sequence data (12.5–17.1 % uncorrected pairwise ND2 sequence divergence) as well as aspects of morphology including the number and arrangement of precloacal pores in males, the number of mid-ventral scales and paravertebral tubercles, and colour pattern. This is the second Cyrtodactylus endemic to the Guwahati region, the fourth from Assam and the twelfth from Northeast India. 

Cymbidium yunnanensis: a new orchid species (Orchidaceae; Epidendroideae) from China based on morphological and molecular evidence

Phytotaxa ◽  
2019 ◽  
Vol 387 (2) ◽  
pp. 149-157 ◽  
Author(s):  
GUO-QIANG ZHANG ◽  
GUI-ZHEN CHEN ◽  
LI-JUN CHEN ◽  
SI-REN LAN
Keyword(s):  
New Species ◽  
Dna Sequence ◽  
Yunnan Province ◽  
Sequence Data ◽  
Phylogenetic Analyses ◽  
Molecular Evidence ◽  
Orchid Species ◽  
Molecular Analyses ◽  
Dna Sequence Data ◽  
A New Species

A new orchid species, Cymbidium yunnanensis (Orchidaceae; Epidendroideae; Cymbidieae), from Yunnan Province, China, is described and illustrated. Based on morphological and molecular analyses, we demonstrate that this new species is similar to C. floribundum, but it differs in its smaller size, leaves 5–15 cm, shorter scape, white flowers with pale purplish markings on its sepals and petals, lip with purplish red blotch, petals narrowly falcate oblong and lip midlobe nearly round with column foot 1.5–2.0 mm long. Phylogenetic analyses based on nuclear ribosomal ITS and plastid (matK, rbcL) DNA sequence data support C. yunnanensis as a new species.

The discovery and description of a new species of Bent-toed Gecko of  the Cyrtodactylus pulchellus complex (Squamata: Gekkonidae) from the Langkawi Archipelago, Kedah, Peninsular Malaysia

Zootaxa ◽  
2019 ◽  
Vol 4668 (1) ◽  
pp. 51-75 ◽  
Author(s):  
EVAN S. H. QUAH ◽  
L. LEE GRISMER ◽  
PERRY L. JR WOOD ◽  
SHAHRUL ANUAR MOHD SAH
Keyword(s):  
New Species ◽  
Mitochondrial Gene ◽  
Color Pattern ◽  
Peninsular Malaysia ◽  
Northwest Coast ◽  
Molecular Evidence ◽  
The Third ◽  
The Many ◽  
A New Species

A new species of limestone karst-adapted gecko of the Cyrtodactylus pulchellus complex, C. dayangbuntingensis sp. nov., is described from Dayang Bunting Island of the Langkawi Archipelago off the northwest coast of Peninsular Malaysia. It is the third species of the group to be described from the archipelago after C. langkawiensis and C. macrotuberculatus. The new species can be distinguished from all other species of Cyrtodactylus based on molecular evidence from the mitochondrial gene ND2 and its flanking tRNAs as well as having unique combinations of morphological and color pattern characteristics. This discovery underscores the need for continued surveys of the many islands in the archipelago to properly ascertain its true herpetological diversity. 

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