Deep into darkness: the first stygobitic species of freshwater shrimp of the genus Caridina (Crustacea: Decapoda: Atyidae) from Northern Vietnam

Zootaxa ◽  
2021 ◽  
Vol 4933 (3) ◽  
pp. 422-434
Author(s):  
DO VAN TU ◽  
CAO THI KIM THU ◽  
THOMAS VON RINTELEN

The first stygobitic species of atyid freshwater shrimps from Vietnam is described from Cuc Phuong National Park, Northern Vietnam. Caridina thachlam sp. nov. can be distinguished from its congeners by a suite of morphological characters such as strongly reduced eyes without eyestalk, short and almost unarmed rostrum and slender pereiopods. Molecular phylogenetic data from the mitochondrial cytochrome oxidase subunit I (COI) and 16S ribosomal RNA genes also support the distinctiveness of the new species from all other species that we have examined. 

Zootaxa ◽  
2021 ◽  
Vol 4999 (3) ◽  
pp. 228-242
Author(s):  
DO VAN TU ◽  
DANG VAN DONG ◽  
THOMAS VON RINTELEN

A new species of atyid freshwater shrimp is described from two caves of Bac Kan Province, Northeast Vietnam, namely Caridina namdat sp. nov. This species can be distinguished from its congeners by a suite of morphological characters such as reduced eyes with short eyestalk, very short and unarmed rostrum, slender second pereiopod, elongated endopod of male first pleopod, and long appendix interna of the male second pleopod. Molecular phylogenetic data from the mitochondrial cytochrome oxidase subunit I (COI) and 16S ribosomal RNA genes also support the distinctiveness of the new species from all other examined species.


ZooKeys ◽  
2019 ◽  
Vol 877 ◽  
pp. 1-29
Author(s):  
Hsi-Te Shih ◽  
Jhih-Wei Hsu ◽  
Kingsley J. H. Wong ◽  
Ngan Kee Ng

Intertidal mudflat crabs of the genus Metaplax H. Milne Edwards, 1852 (Crustacea: Brachyura: Varunidae) from China, Taiwan, and northern Vietnam are taxonomically revised by morphological and molecular evidence. These crabs show sexual dimorphism and morphological variation of a considerable range in the infraorbital ridge, one of the primary features previously used for species identification. In this study, four species were identified from the region: M. elegans De Man, 1888; M. longipes Stimpson, 1858; M. sheni Gordon, 1930; and M. tredecim Tweedie, 1950. Based on the results of the morphological examination, and as confirmed by molecular evidence from mitochondrial cytochrome oxidase subunit I (COI), taxonomic confusion surrounding M. longipes was resolved, and M. takahasii Sakai, 1939, is considered a junior synonym of M. longipes. The geographical distribution of Metaplax longipes extends along the shores of China, north to Jiangsu, whereas the Southeast Asian M. tredecim was newly recorded from northern Vietnam and Hong Kong.


2019 ◽  
Vol 28 (2) ◽  
pp. 258-265
Author(s):  
Amer Ragheb Abdelaziz ◽  
Reda Elbastawisy Khalafalla ◽  
Amal Abbas Abdelrahman Hassan ◽  
Ehab Kotb Elmahallawy ◽  
Abdulaziz Mohammed Almuzaini

Abstract Cysticercus ovis or sheep measles is the larval stage of Taenia ovis, which is the intestinal tapeworm of dogs. It is found in the cardiac and skeletal muscles of sheep and can be the cause of partial or total condemnation of carcasses at abattoirs. The aim of the current work was to determine the prevalence of C. ovis among sheep in Upper Egypt and to present the molecular and phylogenetic analysis of this using the amplified Mitochondrial Cytochrome Oxidase subunit 1 (MT-CO1) gene. A total of 1885 sheep slaughtered at local abattoirs of 4 different governorates of Upper Egypt (Asuit, Sohag, Qena and Aswan) were carefully examined for C. ovis. The overall prevalence of infection was 2.02%. The highest rate of infection was observed in adult animals over 4 years of age (44.73%). There was no significant effect of animal sex on infection rates. The phylogenic analysis of C. ovis Egyptian isolates showed very close similarity to the New Zealand isolate (AB731675). This is the first report showing the genetic analysis of C. ovis in Egypt, which provides a very powerful tool for taxonomy and definitive diagnosis of C. ovis, which could be helpful for preventive and control programs.


Nematology ◽  
2017 ◽  
Vol 19 (3) ◽  
pp. 323-331 ◽  
Author(s):  
Rina Sriwati ◽  
Yuko Takeuchi-Kaneko ◽  
J. Jauharlina ◽  
Natsumi Kanzaki

Aphelenchoidid nematodes associated with the syconia of two dominant fig species,Ficus hispidaandF. racemosa, were surveyed in Banda Aceh, Indonesia. Nematodes were isolated from sycones and pollinating wasps of these two fig species from four localities in the area, and identified based on the molecular sequences of two genetic loci, D2-D3 expansion segments of large subunit ribosomal RNA (D2-D3 LSU) and mitochondrial cytochrome oxidase subunit I (mtCOI). Molecular sequences of D2-D3 LSU and mtCOI were successfully determined for 44 and 19 individual nematodes, respectively, and these sequences were separated into four clades,i.e., types A-D of D2-D3 LSU and types I-IV of mtCOI. Phylogenetic analysis of the DNA sequences deposited in the GenBank database showed that the DNA sequences corresponded to three species, namely,Martininema baculum(type B/II),Ficophagus fleckeri(types A/I, D/IV) andF. cf.centerae(type C/III). Within these species,F. fleckeriwas separated into two clades as suggested in previous studies and thus it may possibly reflect the existence of two different taxa,F. fleckeriand a cryptic species. The IndonesianF. cf.centeraewas monophyletic with, but clearly separated from, the Chinese population ofF. centeraeand thus the Indonesian population is potentially an undescribed species. Overall, the species composition of fig-associated aphelenchoidids in the Aceh region seemed intermediate between continental Chinese and Australian species. However, further material collections followed by detailed morphological analyses are necessary to characterise or describe these fig-associated aphelenchoidids in Indonesia.


Zootaxa ◽  
2010 ◽  
Vol 2670 (1) ◽  
pp. 59 ◽  
Author(s):  
FEDOR ČIAMPOR JR. ◽  
JÁN KODADA

Two mitochondrial (cytochrome oxidase subunit I, 16S RNA) and one nuclear (18S RNA) gene fragments were used to analyse relationships within the Oulimnius tuberculatus species group. The results of molecular data analysis are compared with morphological characters, and the distributional patterns and taxonomic status of recognized taxa are addressed. Based on molecular data, Oulimnius perezi is re-validated as a distinct species, and the discovery of a possible new species from northwest Italy is reported. The possible origins and times of speciation within the species group are discussed.


2013 ◽  
Vol 103 (4) ◽  
pp. 451-457 ◽  
Author(s):  
Andjeljko Petrović ◽  
Milana Mitrović ◽  
Petr Starý ◽  
Olivera Petrović-Obradović ◽  
Vladimir Žikić ◽  
...  

AbstractWe report the occurrence of Lysiphlebus orientalis in Serbia, an aphid parasitoid from the Far East that is new to Europe and has the potential to become invasive. Our finding based on morphological characters is confirmed by analyses of mitochondrial cytochrome oxidase subunit I sequences. An increase in number and an expansion of the host range were observed during field studies over the past two years, and it is determined that the current host range encompasses nine aphid hosts on 12 different host plants, forming 13 tri-trophic associations. A host range determined for European populations of L. orientalis appears wider compared with that in its Far Eastern native habitats where Aphis glycines Mats. is the sole known host. Moreover, it overlaps considerably with the host ranges of European parasitoids that play an important role in the natural control of pest aphids.


PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e6371 ◽  
Author(s):  
Jan Vicente ◽  
Jaime Andrés Ríos ◽  
Sven Zea ◽  
Robert J. Toonen

Neopetrosia proxima(Porifera: Demospongiae: Haplosclerida) is described as a morphologically variable sponge common on shallow reefs of the Caribbean. However, the range of morphological and reproductive variation within putativeN. proximaled us to hypothesize that such variability may be indicative of cryptic species rather than plasticity. Using DNA sequences and morphological characters we confirmed the presence of three previously undescribed species ofNeopetrosia. Morphological differences of each new congener were best resolved by partial gene sequences of the mitochondrial cytochrome oxidase subunit 1 over nuclear ones (18S rRNA and 28S rRNA). Several new characters forNeopetrosiawere revealed by each new species. For example,N. dendrocrevaceasp. nov. andN. cristatasp. nov. showed the presence of grooves on the surface of the sponge body that converge at the oscula, and a more disorganized skeleton than previously defined for the genus.N. sigmaferasp. nov. adds the (1) presence of sigma microscleres, (2) significantly wider/longer oxeas (>200 μm), and (3) the presence of parenchymella larvae. Sampling of conspecifics throughout several locations in the Caribbean revealed larger spicules in habitats closer to the continental shelf than those in remote island locations. Our study highlights the importance of integrating molecular and morphological systematics for the discrimination of newNeopetrosiaspp. despite belonging to one of several polyphyletic groups (families, genera) within the current definition of the order Haplosclerida.


2020 ◽  
Vol 69 (3) ◽  
pp. 283-288
Author(s):  
Miloš Černý ◽  
Jiří Kocián ◽  
Jan Ševčík

Abstract A male of Chromatomyia aizoon (Hering, 1932) (Diptera: Agromyzidae) was reared from a leaf mine at Saxifraga paniculata collected in Štramberk (Northern Moravia, Czech Republic). Partial sequence of the mitochondrial cytochrome oxidase subunit I (COI barcode region, 658 bp) is also provided.


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