Molecular phylogenetics, PCA, and MFA recover a new species of Cyrtodactylus (Squamata: Gekkonidae) from an isolated sandstone massif in northwestern Cambodia

Zootaxa ◽  
2021 ◽  
Vol 4949 (2) ◽  
pp. 261-288
Author(s):  
L. LEE GRISMER ◽  
PETER GEISSLER ◽  
THY NEANG ◽  
TIMO HARTMANN ◽  
PHILIPP WAGNER ◽  
...  
Keyword(s):  
Molecular Phylogenetics ◽  
Mitochondrial Gene ◽  
Principal Component ◽  
Field Work ◽  
Color Pattern ◽  
Species Group ◽  
Multiple Factor Analysis ◽  
Base Pairs ◽  
Nadh Dehydrogenase Subunit 2 ◽  
Habitat Island

The integrated results of maximum likelihood (ML) and Bayesian inference (BI) analyses, principal component analyses (PCA), and a multiple factor analysis (MFA) recover a new, widely allopatric species of the Cyrtodactylus intermedius species group. Cyrtodactylus kulenensis sp. nov is endemic to the Phnom Kulen sandstone massif of the Phnom Kulen National Park, Siem Reap Province, in the lowlands of northwestern Cambodia. A phylogenetic analysis from a short read (275 base pairs) of the mitochondrial gene NADH dehydrogenase subunit 2 (ND2) from C. kulenensis sp. nov. was aligned with 1449 base pairs from all other species in the intermedius group.  The analysis recovered C. kulenensis sp. nov. as the sister species to a lineage composed of populations from the widely separated hilly regions of Sa Keao and Sakaerat in eastern Thailand. Multivariate (PCA, DAPC, and MFA) and univariate analyses (ANOVA) using combinations of meristic (scale counts), mensural (morphometric), and categorical (color pattern and morphology) characters from 52 specimens encompassing all species of the intermedius group clearly demonstrate C. kulenensis sp. nov. is significantly different and discretely diagnosable from all other species in the intermedius group. This new discovery further highlights the herpetological diversity and high levels of range-restricted endemism in basin-habitat-island landscapes throughout Indochina and the continued need for field work in the landscapes that remain unsurveyed. 

scholarly journals A new cryptic arboreal species of the Cyrtodactylus brevipalmatus group (Squamata: Gekkonidae) from the uplands of western Thailand

2021 ◽  
Vol 71 ◽  
pp. 723-746
Author(s):  
L. Lee Grismer ◽  
Chatmongkon Suwannapoom ◽  
Parinya Pawangkhanant ◽  
Roman A. Nazarov ◽  
Platon V. Yushchenko ◽  
...  
Keyword(s):  
New Species ◽  
Mitochondrial Gene ◽  
Sequence Divergence ◽  
Field Work ◽  
Morphological Characters ◽  
Sister Species ◽  
Base Pairs ◽  
Meristic Characters ◽  
Nadh Dehydrogenase Subunit 2 ◽  
A New Species

The first integrative taxonomic analysis of the Cyrtodactylus brevipalmatus group of Southeast Asia recovered two newly discovered populations from the Tenasserim Mountains in Suan Phueng District, Ratchaburi Province, Thailand as a new species described here as C. rukhadeva sp. nov. Based on 1397 base pairs of the mitochondrial gene NADH dehydrogenase subunit 2 (ND2), C. rukhadeva sp. nov. is the well-supported sister species to a clade containing three undescribed species, C. ngati, and C. cf. interdigitalis with a large uncorrected pairwise sequence divergence from other species in the brevipalmatus group ranging from 15.4–22.1%. Cyrtodactylus elok and C. brevipalmatus are recovered as poorly supported sister species and the well-supported sister lineage to the remainder of the brevipalmatus group. Cyrtodactylus rukhadeva sp. nov. is putatively diagnosable on the basis of a number of meristic characters and easily separated from the remaining species of the brevipalmatus group by a number of discrete morphological characters as well as its statistically significant wide separation in multivariate morphospace. The discovery of C. rukhadeva sp. nov. continues to underscore the unrealized herpetological diversity in the upland forests of the Tenasserim Mountains and that additional field work will undoubtedly result in the discovery of additional new species.

scholarly journals A molecular and morphological investigation of species boundaries and phylogenetic relationships in Australian free-tailed bats Mormopterus (Chiroptera : Molossidae)

2014 ◽  
Vol 62 (2) ◽  
pp. 109 ◽  
Author(s):  
T. B. Reardon ◽  
N. L. McKenzie ◽  
S. J. B. Cooper ◽  
B. Appleton ◽  
S. Carthew ◽  
...  
Keyword(s):  
Phylogenetic Analyses ◽  
Mitochondrial Gene ◽  
Phylogeographic Structure ◽  
Species Boundaries ◽  
Base Pairs ◽  
Nadh Dehydrogenase Subunit 2 ◽  
The Family ◽  
Taxonomic Uncertainty ◽  
History Of ◽  
Phylogenetic Affinities

The taxonomic uncertainty surrounding several prominent genera of Australian microbat has been a long-standing impediment to research and conservation efforts on these groups. The free-tail bat genus Mormopterus is perhaps the most significant example, with a long history of acknowledged species-level confusion. This study uses a combined molecular and morphological approach to conduct a comprehensive assessment of species and subgeneric boundaries, between-species phylogenetic affinities and within-species phylogeographic structure in Australian members of Mormopterus. Phylogenetic analyses based on 759 base pairs of the NADH Dehydrogenase subunit 2 mitochondrial gene were concordant with species boundaries delineated using an expanded allozyme dataset and by phallic morphology, and also revealed strong phylogeographic structure within two species. The levels of divergence evident in the molecular and morphological analyses led us to recognise three subgenera within Australia: Micronomus, Setirostris subgen. nov. and Ozimops subgen. nov. Within Ozimops we recognise seven Australian species, three of which are new, and none are conspecific with Indo-Papuan species. The family Molossidae now comprises eleven species across three subgenera in Australia, making it the continent’s second most speciose family of bats.

A new species of Cyrtodactylus Gray (Squamata; Gekkonidae) from the Thai Highlands with a discussion on the evolution of habitat preference

Zootaxa ◽  
2020 ◽  
Vol 4852 (4) ◽  
pp. 401-427
Author(s):  
L. LEE GRISMER ◽  
ATTAPOL RUJIRAWAN ◽  
KORKHWAN TERMPRAYOON ◽  
NATEE AMPAI ◽  
SIRIPORN YODTHONG ◽  
...  
Keyword(s):  
New Species ◽  
Habitat Preference ◽  
Phylogenetic Analyses ◽  
Mitochondrial Gene ◽  
Sequence Divergence ◽  
Color Pattern ◽  
Nadh Dehydrogenase Subunit 2 ◽  
Collection Data ◽  
Habitat Generalist ◽  
Clade 1

A new gekkonid lizard, Cyrtodactylus maelanoi sp. nov., from Mae Hong Son Province of the Thai Highlands is described using an integrative taxonomic analysis based on morphology, color pattern, and the mitochondrial gene NADH dehydrogenase subunit 2 (ND2) and its flanking tRNAs. Phylogenetic analyses place the new species within clade 1 of the C. sinyineensis group and as the sister species to C. inthanon with an uncorrected pairwise sequence divergence of 3.9%. Collection data gathered in the field indicate that C. maelanoi sp. nov. is a habitat generalist. Reconstruction of the ancestral habitat preference for the C. sinyineensis group by way of stochasitc character mapping (SCM) indicates that karstic environments were the ancestral condition out of which the general habitat preference of the ancestor of C. maelanoi sp. nov. and C. inthanon and that of C. amphipetreaus and C. doisuthep evolved three times independently. Additionally, SCM demonstrated that the evolution of a granitic habitat preference from a karst-adapted ancestor happened in C. aequalis. The discovery of a new upland species in the Thai Highlands brings into focus the understudied nature of the mountain systems of western Thailand and the need for their continued exploration and conservation. 

A new species Cyrtodactytlus Gray (Squamata: Gekkonidae) from western Thailand and the phylogenetic placement of C. inthanon and C. doisuthep

Zootaxa ◽  
2020 ◽  
Vol 4838 (2) ◽  
pp. 179-209
Author(s):  
SIRIWADEE CHOMDEJ ◽  
CHATMONGKON SUWANNAPOOM ◽  
PARINYA PAWANGKHANANT ◽  
WARANEE PRADIT ◽  
ROMAN A. NAZAROV ◽  
...  
Keyword(s):  
New Species ◽  
Nadh Dehydrogenase ◽  
Phylogenetic Analyses ◽  
Mitochondrial Gene ◽  
Color Pattern ◽  
Nadh Dehydrogenase Subunit ◽  
Taxonomic Analysis ◽  
Nadh Dehydrogenase Subunit 2 ◽  
Tak Province ◽  
A New Species

A new species of Cyrtodactylus from Tak Province, Thailand, Cyrtodactylus amphipetraeus sp. nov., is described using an integrative taxonomic analysis based on morphology, color pattern, and the mitochondrial gene NADH dehydrogenase subunit 2 (ND2). The phylogenetic analyses place the new species within the C. sinyineensis group which was previously thought to be endemic to the Salween Basin in southern Myanmar. The phylogeny also places C. inthanon in the C. sinyneensis group which is expanded herein to also include the group’s sister species C. doisuthep. Along with C. amphipetraeus sp. nov., these are the first three species of the C. sinyineensis group to be found outside of Myanmar east of the Tenasserim Mountains. The Tenasserim Mountain region is discussed as an area of cladogeneic turnover. 

A re-description of Cyrtodactylus chrysopylos Bauer (Squamata: Gekkonidae) with comments on the adaptive significance of orange coloration in hatchlings and descriptions of two new species from eastern Myanmar (Burma)

Zootaxa ◽  
2018 ◽  
Vol 4527 (2) ◽  
pp. 151 ◽  
Author(s):  
L. LEE GRISMER ◽  
PERRY L., JR. WOOD ◽  
MYINT KYAW THURA ◽  
NAY MYO WIN ◽  
MARTA S. GRISMER ◽  
...  
Keyword(s):  
New Species ◽  
Mitochondrial Gene ◽  
Color Pattern ◽  
Sister Species ◽  
Base Pairs ◽  
Bright Orange ◽  
Cave Population ◽  
Intraspecific Communication ◽  
Associated Species ◽  
The Village

The karstic foothills and hilly western edge of the Shan Plateau of eastern Myanmar continue to be sources of discovery for new species of the gekkonid genus Cyrtodactylus. Two new karst-associated species within this unique landscape from Mandalay Region—C. aunglini sp. nov. from the Kyauk Nagar Cave and C. myaleiktaung sp. nov. from Mya Leik Taung Mountain—are described on the basis of color pattern, morphology, and genetics. A molecular phylogeny of the C. gansi group (defined herein) based on 1481 base pairs of the mitochondrial gene ND2 and its flanking tRNAs place C. aunglini sp. nov. as the sister species to C. gansi and C. myaleiktaung sp. nov. as the sister species of C. chrysopylos. A new population of C. chrysopylos from a boulder-strewn, karstic ridge near the village of Yane, Shan State at an elevation 951 m is reported here. Genetic and geographic variation as well as differences in natural history between it and the lowland cave population of C. chrysopylos from the type locality in the Panlaung-Pyadalin Cave Wildlife Sanctuary, Shan State at 303 m are discussed. Hatchlings of the new population from Yane are nearly uniformly bright-orange in color. We provide evidence in support of a hypothesis claiming this color functions as a mechanism for crypsis during low levels of illumination and is not involved in intraspecific communication. 

Geographically structured genetic variation in Ptychozoon  lionotum (Squamata: Gekkonidae) and a new species from an isolated volcano in Myanmar

Zootaxa ◽  
2018 ◽  
Vol 4514 (2) ◽  
pp. 202 ◽  
Author(s):  
L. LEE GRISMER ◽  
PERRY L. JR. WOOD ◽  
MYINT KYAW THURA ◽  
MARTA S. GRISMER ◽  
RAFE M. BROWN ◽  
...  
Keyword(s):  
Genetic Variation ◽  
New Species ◽  
Mitochondrial Gene ◽  
Sequence Divergence ◽  
Color Pattern ◽  
Genetic Lineages ◽  
Molecular Phylogenetic ◽  
Habitat Island ◽  
Northern Portion ◽  
A New Species

A molecular phylogenetic analysis of Parachute Geckos (Genus Ptychozoon Kuhl & van Hasselt, 1822) based on the mitochondrial gene ND2 indicates that a newly discovered population from the Mt. Popa volcano—a habitat island in the northern portion of the Bago Yoma mountains, Myanmar—is a new species, P. popaense sp. nov. This species is part of a clade that contains P. bannanense Wang, Wang, & Liu, 2016 and P. lionotum Annandale, 1905 of Indochina. Ptychozoon popaense sp. nov. is morphologically most similar to its sister species P. lionotum  which manifests considerable geographic substructuring of genetic variation but differs from the nominate taxon by an uncorrected pairwise sequence divergence of 16.0–17.1% and by discrete differences in morphology and color pattern. This discovery highlights the unique, insular nature of the Bago Yoma mountains of the Ayeyarwady Basin, which support other endemic gekkonids. It also underscores the growing diversity in this highly derived clade of cryptic, parachuting, geckos characterized by highly divergent genetic lineages, which may indicate the presence of additional, unrecognized species. 

Two new species of Cyrtodactylus Gray, 1827 (Squamata: Gekkonidae) from a karstic archipelago in the Salween Basin of southern Myanmar (Burma)

Zootaxa ◽  
2020 ◽  
Vol 4718 (2) ◽  
pp. 151-183 ◽  
Author(s):  
L. LEE GRISMER ◽  
PERRY L. JR. WOOD ◽  
EVAN S. H. QUAH ◽  
MARTA S. GRISMER ◽  
MYINT KYAW THURA ◽  
...  
Keyword(s):  
New Species ◽  
Mitochondrial Gene ◽  
Small Region ◽  
Color Pattern ◽  
Sister Species ◽  
Nadh Dehydrogenase Subunit 2 ◽  
Habitat Islands ◽  
Mean Differences ◽  
High Degree ◽  
Group Species

An integrative taxonomic analysis of the 10 species of the Cyrtodactylus sinyineensis group based on squamation, color pattern, and the mitochondrial gene NADH dehydrogenase subunit 2 (ND2) and its flanking tRNA regions, recovered the newly discovered populations from Datt Kyaik and Taung Wine Hills in Kayin State as the new species Cyrtodactylus dattkyaikensis sp. nov. and C. taungwineensis sp. nov. The Maximum Likelihood and Bayesian phylogenetic estimates supported C. dattkyaikensis sp. nov. as the sister species of C. bayinnyiensis and C. taungwineensis sp. nov. as the sister species of C. sinyineensis. Each new species is differentially diagnosable from all other C. sinyineensis group species based on their morphological placement in multivariate space and several statistically significant mean differences is meristic squamation and color pattern data. The C. sinyineensis group ranges across an archipelago of karstic habitat-islands in the Salween Basin of southern Myanmar. The discovery of these new species continues to underscore the unprecedented high degree of diversity and site-specific endemism in this relatively small region and the urgent need for the conservation of its karstic terranes. 

scholarly journals A phylogenetic taxonomy of the Cyrtodactylus peguensis group (Reptilia: Squamata: Gekkonidae) with descriptions of two new species from Myanmar

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e5575 ◽  
Author(s):  
L. Lee Grismer ◽  
Perry L. Wood ◽  
Evan S.H. Quah ◽  
Matthew L. Murdoch ◽  
Marta S. Grismer ◽  
...  
Keyword(s):  
New Species ◽  
Nadh Dehydrogenase ◽  
Mitochondrial Gene ◽  
Color Pattern ◽  
Type Locality ◽  
Nadh Dehydrogenase Subunit ◽  
Molecular Systematic ◽  
Phylogenetic Taxonomy ◽  
Nadh Dehydrogenase Subunit 2 ◽  
Two New Species

A phylogenetic taxonomy of species in the Cyrtodactylus peguensis group from the Ayeyarwady Basin of Myanmar is constructed based on color pattern, morphology, and molecular systematic analyses using the mitochondrial gene NADH dehydrogenase subunit 2. Newly collected samples from the type locality of C. peguensis and other localities indicate that this clade is endemic to central Myanmar and contains at least seven species, four of which are undescribed. Three species, including C. peguensis occur in the low hills of the Bago Yoma Range within the central portion of the Ayeyarwady Basin. Two of these, C. myintkyawthurai sp. nov. from the northern and central Bago Yoma and C. meersi sp. nov. which is syntopic with C. peguensis in the southern Bago Yoma are described herein. As more lowland hilly areas bordering, and within the Ayeyarwady Basin are surveyed, more new species of this group are likely to be discovered. These discoveries continue the recent surge of descriptions of new species of Cyrtodactylus that are being discovered in Myanmar.

Six new species of the Cyrtodactylus intermedius complex (Squamata: Gekkonidae) from the Cardamom Mountains and associated highlands of Southeast Asia

Zootaxa ◽  
2019 ◽  
Vol 4554 (1) ◽  
pp. 1 ◽  
Author(s):  
MATTHEW L. MURDOCH ◽  
L. LEE GRISMER ◽  
PERRY L. JR. WOOD ◽  
THY NEANG ◽  
NIKOLAY A. POYARKOV ◽  
...  
Keyword(s):  
New Species ◽  
Mitochondrial Gene ◽  
Sequence Divergence ◽  
Color Pattern ◽  
Sensu Stricto ◽  
Species Level ◽  
Base Pairs ◽  
Taxonomic Analysis ◽  
Karst Formation ◽  
Geographic Regions

An integrative taxonomic analysis using color pattern, morphology, and 1449 base pairs of the ND2 mitochondrial gene and its five flanking tRNAs demonstrated that eight species-level lineages occur within the Cyrtodactylus intermedius complex (Cyrtodactylus intermedius sensu stricto, C. phuquocensis and related populations) of the Cardamom mountains and associated highlands that have a sequence divergence ranging 3.4–8.9%. Additionally, each lineage is discretely diagnosable from one another based on morphology and color pattern and most occur in specific geographic regions (upland areas, karst formations or islands) that prevent or greatly restrict interpopulation gene flow. Six of these lineages were masquerading under the nomen C. intermedius and are described as the following: Cyrtodactylus auralensis sp. nov. endemic to Phnom Aural, the highest mountain in Cambodia; C. bokorensis sp. nov. endemic to the Bokor Plateau, Cambodia; C. cardamomensis sp. nov. from the main block of the Cardamom mountains; C. thylacodactylus sp. nov. endemic to Phnom Dalai the northernmost peak of the Cardamom mountains; C. laangensis sp. nov. endemic to the Phnom Laang karst formation, Cambodia; and C. septimontium sp. nov. from the Bảy Núi Hills of southwest Vietnam. 

Four new Burmese species of Hemiphyllodactylus Bleeker (Squamata: Gekkonidae) from distantly related parapatric clades from the Shan Plateau and Salween Basin

Zootaxa ◽  
2020 ◽  
Vol 4758 (1) ◽  
pp. 45-82 ◽  
Author(s):  
L. LEE GRISMER ◽  
PERRY L. JR. WOOD ◽  
EVAN S. H. QUAH ◽  
MYINT KYAW THURA ◽  
JAMIE R. OAKS ◽  
...  
Keyword(s):  
New Species ◽  
Cloud Forest ◽  
Mitochondrial Gene ◽  
Color Pattern ◽  
The South ◽  
Taxonomic Analysis ◽  
The North ◽  
North And South ◽  
Northwestern Thailand

An integrative taxonomic analysis based on morphology, color pattern, and the mitochondrial gene ND2 recovered four new species of Hemiphyllodactylus Bleeker that are endemic to the Shan Plateau or Salween Basin in eastern Myanmar. Hemiphyllodactylus ngwelwini sp. nov. from the Shan Plateau is part of the earlier described “eastern Myanmar clade” renamed herein as the north lineage and H. kyaiktiyoensis sp. nov. and H. pinlaungensis sp. nov. of the Shan Plateau and H. zwegabinensis sp. nov. of the Salween Basin compose an entirely new Burmese clade herein referred to as the south lineage. Although the north and south lineages come within 46 km of one another on the Shan Plateau, they are not sister lineages but sequentially separated by two lineages from Yunnan, China and another from northwestern Thailand. Hemiphyllodactylus zwegabinensis sp. nov. is the first species of this genus to be recorded from the Salween Basin and is known only from a wind-blown cloud forest on the top of the insular, karstic mountain Zwegabin in Kayin State. All other Burmese species except for H. typus, are endemic to the various localities throughout the Shan Plateau. These four new species bring the total number of Hemiphyllodactylus in Myanmar to at least 10 which is certainly an extreme underestimate of the diversity of this genus given that we discover new species at every upland locality we survey. 

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