Construction of Hyaluronic Acid-CeO2 Conjugated Composite Nanoparticles and Their Activity Efficiency in Diabetic Retinopathy Alleviation

2021 ◽  
Vol 17 (11) ◽  
pp. 2219-2225
Author(s):  
Jingzhi Shao ◽  
Jingjing Wan ◽  
Fengyan Zhang ◽  
Lirong Zhang
Keyword(s):  
Diabetic Retinopathy ◽  
Hyaluronic Acid ◽  
Mouse Model ◽  
Clinical Efficacy ◽  
Structural Integrity ◽  
Foreign Body Reaction ◽  
Immune Cell ◽  
Cell Infiltration ◽  
Immune Cell Infiltration ◽  
Retinal Structure

We developed an effective nanoparticle-biomaterial in alleviating diabetic retinopathy (DR), hyaluronic acid (HA)-CeO2, composed mainly of CeO2 and HA. To demonstrate its anti-DR capacity, retinal cells from a B6/J mouse model were used to compare the efficiency of PEI-CeO2 and HA-CeO2. We investigated the transport performance, histolysis, immune cell infiltration, angiogenesis, and hyperemia induced by the transport system. The structural integrity, microvascular apoptosis, and superoxide and peroxide concentrations in the retina were measured to evaluate the clinical efficacy of CeO2. The infiltration efficiency of HA-CeO2 was higher than that of PEI-CeO2. Lower levels of foreign body reaction were evident for HA-CeO2 with less histolysis, immune cell infiltration, angiogenesis, and hyperemia. The clinical efficacy of HA-CeO2 in terms of preservation of retinal structure and lowering of microvascular apoptosis and superoxide and peroxide concentrations was superior to those of PEI-CP. HA-CeO2 was shown to have significant antioxidation and anti-vascular injury capacity in a mouse model, and may be a potential compound nanodrug for DR treatment in the future.

Effects of ASCL1 expression on tumorigenesis and immune cell infiltration in a syngenic mouse model of lung adenocarcinoma

2019 ◽  
Author(s):  
Naoya Miyashita ◽  
Masafumi Horie ◽  
Yu Mikami ◽  
Hirokazu Urushiyama ◽  
Kensuke Fukuda ◽  
...  
Keyword(s):  
Mouse Model ◽  
Lung Adenocarcinoma ◽  
Immune Cell ◽  
Cell Infiltration ◽  
Immune Cell Infiltration

Hyperglycemia results in decreased immune cell infiltration and increased viral load in the lung in a mouse model of RSV infection

Cytokine ◽  
2021 ◽  
pp. 155539
Author(s):  
Farshad Khodakhah ◽  
Alireza Tahamtan ◽  
Mona Marzban ◽  
Azadeh Shadab ◽  
Masoumeh Tavakoli-Yaraki ◽  
...  
Keyword(s):  
Viral Load ◽  
Mouse Model ◽  
Immune Cell ◽  
Cell Infiltration ◽  
Immune Cell Infiltration ◽  
Rsv Infection

scholarly journals Selective In Vivo Visualization of Immune-Cell Infiltration in a Mouse Model of Autoimmune Myocarditis by Fluorine-19 Cardiac Magnetic Resonance

2013 ◽  
Vol 6 (2) ◽  
pp. 277-284 ◽  
Author(s):  
Ruud B. van Heeswijk ◽  
Jonathan De Blois ◽  
Gabriela Kania ◽  
Christine Gonzales ◽  
Przemyslaw Blyszczuk ◽  
...  
Keyword(s):  
Cardiac Magnetic Resonance ◽  
Magnetic Resonance ◽  
Mouse Model ◽  
Immune Cell ◽  
Cell Infiltration ◽  
Immune Cell Infiltration ◽  
Autoimmune Myocarditis

scholarly journals Changes in lung immune cell infiltrates after electric field treatment in mice

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Sophia I. Eliseeva ◽  
Zackery A. Knowlden ◽  
Gillian MSchiralli Lester ◽  
David A. Dean ◽  
Steve N. Georas ◽  
...  
Keyword(s):  
Lung Function ◽  
Mouse Model ◽  
Electric Field ◽  
Electric Fields ◽  
Immune Cell ◽  
Immune Therapy ◽  
Allergen Challenge ◽  
Cell Infiltration ◽  
Immune Cell Infiltration ◽  
Electric Field Treatment

AbstractExogenous electric fields are currently used in human therapy in a number of contexts. Interestingly, electric fields have also been shown to alter migration and function of immune cells, suggesting the potential for electric field-based immune therapy. Little is known as to the effect of electric field treatment (EFT) on the lung. To determine if EFT associates with changes in lung immune cell infiltration, we used a mouse model with varying methods of EFT application and measured cells and soluble mediators using flow cytometry and cytokine/chemokine multiplex. EFT was associated with a transient increase in lung neutrophils and decrease in eosinophils in naïve mice within 2 h of treatment, accompanied by an increase in IL-6 levels. In order to test whether EFT could alter eosinophil/neutrophil recruitment in a relevant disease model, a mouse model of allergic airway inflammation was used. Four EFT doses in allergen-sensitized mice resulted in increased neutrophil and reduced eosinophil infiltrates following allergen challenge, suggesting a durable change in inflammation by EFT. Mice with allergic inflammation were analyzed by flexiVent for measures of lung function. EFT-treated mice had increased inspiratory capacity and other measures of lung function were not diminished. These data suggest EFT may be used to manipulate immune cell infiltration in the lung without affecting lung function.

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