MiRNA-136-5p Sensitizes Liver Cancer Cells to Docetaxel by Targeting P53 and Enhances Cell Migration

2022 ◽  
Vol 12 (2) ◽  
pp. 323-328
Author(s):  
Hao Cai ◽  
Jian Du ◽  
Cheng Luo
Keyword(s):  
Liver Cancer ◽  
Cancer Cells ◽  
P53 Expression ◽  
Mirna Array ◽  
Liver Cancer Cells ◽  
Differentially Expressed Mirnas ◽  
Docetaxel Treatment ◽  
Cell Progression ◽  
And Migration ◽  
The Impact

We aimed to explore whether microRNA (miRNA)-136-5p modulates P53 expression, and affects the efficacy of docetaxel treatment for liver cancer. miRNA array screened the differentially expressed miRNAs in biopsy tissues of liver cancer patients, and the expression of miR-136-5p and P53 in tissues and cells by RT-PCR. Following docetaxel treatment, through increased- and decreased-function method, we detected the impact of the miRNA on cell progression, as well as the sensitivity of docetaxel through MTT assay and colony formation experiment. The correlation between miR-136-5p and P53 was evaluated. The expression of miR-136-5p in liver cancer cells is up-regulated, which is consistent with the results of bioinformatics analysis. Further, miR-136-5p overexpression promoted cell proliferation and migration, and sensitized liver cancer cells to docetaxel. Interestingly, P53 was indicated to bind to miR-136-5p, and P53 participated in the up-regulation of MMP10 induced by miR-136-5p. miR-136-5p enhances the sensitivity to docetaxel in liver cancer and thus could be a biomarker for the treatment against liver cancer.

Nuclear expression of the ubiquitin ligase seven in absentia homolog (SIAH)-1 induces proliferation and migration of liver cancer cells

2011 ◽  
Vol 55 (5) ◽  
pp. 1049-1057 ◽  
Author(s):  
Antje Brauckhoff ◽  
Mona Malz ◽  
Darjus Tschaharganeh ◽  
Nisar Malek ◽  
Achim Weber ◽  
...  
Keyword(s):  
Liver Cancer ◽  
Cancer Cells ◽  
Ubiquitin Ligase ◽  
Liver Cancer Cells ◽  
Nuclear Expression ◽  
Seven In Absentia ◽  
And Migration ◽  
Proliferation And Migration

scholarly journals Graphene oxide nanofilm and chicken embryo extract decrease the invasiveness of HepG2 liver cancer cells

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Malwina Sosnowska ◽  
Marta Kutwin ◽  
Barbara Strojny ◽  
Piotr Koczoń ◽  
Jarosław Szczepaniak ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Liver Cancer ◽  
Cancer Cells ◽  
Chicken Embryo ◽  
Liver Extract ◽  
Functional Protein ◽  
Liver Cancer Cells ◽  
Expression Of Genes ◽  
Embryo Liver ◽  
The Impact

Abstract Background The extracellular matrix (ECM) is a mosaic of various structural and functional proteins that cooperate with the cell, regulate adhesion, and consequently manage its further fate. Liver destruction is accompanied by a disruption of the physicochemical properties of the ECM which deregulates the cell–ECM interaction and can lead to uncontrolled proliferation and neoplastic transformation of cells. Therefore, it can be assumed that ECM modification and restoration of its characteristics for healthy tissue may counteract uncontrolled cell proliferation. The purpose of the presented research model was to optimise the physical characteristics of ECM by introducing a graphene oxide plane/nanofilm (nfGO) and enriching the cell environment with potentially missing proteins by adding a functional protein cocktail (chicken embryo liver extract) and determine the impact of these factors on cell–ECM cooperation and its consequences on adhesion, proliferation, and cell phase, which are factors of the invasiveness of cancer cells. Results Experiments were performed with non-cancer HS-5 cells and liver cancer cells HepG2 and C3A. The cells were divided into four groups: (1) control, (2) cultured on nfGO, (3) cultured with the addition of chicken embryo liver extract (CELE) and (4) cultured on the nfGO with the addition of CELE. CELE contained 1735 proteins; the top 57 of these proteins have been presented. The use of nfGO as well as CELE and nfGO + CELE reduced the proliferation of HepG2 cancer cells to the greatest extent; this is in contrast to non-cancer cells and also to C3A cancer cells. Furthermore, the combined use of the CELE protein cocktail and GO substrate effectively resulted in a decrease in the population of HepG2 cells in the G0/G1 phase and an increase of the population in G2/M. Molecular analysis of HepG2 cancer cells also showed an increase in the expression of genes responsible for adhesion such as focal adhesion kinase (fak), e-cadherin, and n-cadherin and a decrease in β-catenin, which is considered a proto-oncogene. Conclusions Studies have shown that both the GO surface structure on which the cells are grown as well as the presence of a multi-component natural cocktail of regulatory proteins, can modify the expression of integrins, increase adhesion and, as a consequence, proliferation and the cell cycle—entering the resting phase. For the first time, it has been documented that hepatic cancer cells of the HepG2 line under the influence of stimuli derived from mimic ECM (graphene oxide) in interaction with a unique protein complex derived from chicken liver embryo decreased of the invasiveness of cancer cells.

scholarly journals LDB2 inhibits proliferation and migration in liver cancer cells by abrogating HEY1 expression

Oncotarget ◽  
2017 ◽  
Vol 8 (55) ◽  
pp. 94440-94449 ◽  
Author(s):  
Hongyang Yu ◽  
Ruichun Jia ◽  
Ling Zhao ◽  
Shigang Song ◽  
Jing Gu ◽  
...  
Keyword(s):  
Liver Cancer ◽  
Cancer Cells ◽  
Liver Cancer Cells ◽  
And Migration ◽  
Proliferation And Migration

Overexpression of far upstream element binding proteins: A mechanism regulating proliferation and migration in liver cancer cells

Hepatology ◽  
2009 ◽  
Vol 50 (4) ◽  
pp. 1130-1139 ◽  
Author(s):  
Mona Malz ◽  
Achim Weber ◽  
Stephan Singer ◽  
Vera Riehmer ◽  
Michaela Bissinger ◽  
...  
Keyword(s):  
Liver Cancer ◽  
Cancer Cells ◽  
Binding Proteins ◽  
Liver Cancer Cells ◽  
Upstream Element ◽  
And Migration ◽  
Proliferation And Migration

scholarly journals Effects of propofol on the proliferation and migration of liver cancer cells

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Chan Li ◽  
Qingxia Fu ◽  
Jin Cai ◽  
Hongxia Mei ◽  
Wangning Shangguan
Keyword(s):  
Liver Cancer ◽  
Cancer Cells ◽  
Liver Cancer Cells ◽  
And Migration ◽  
Proliferation And Migration

scholarly journals Graphene Oxide Nanofilm and Chicken Embryo Extract Decrease the Invasiveness of HepG2 Liver Cancer Cells

2020 ◽  
Author(s):  
Malwina Sosnowska ◽  
Marta Kutwin ◽  
Barbara Strojny ◽  
Piotr Koczoń ◽  
Jarosław Szczepaniak ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Liver Cancer ◽  
Cancer Cells ◽  
Chicken Embryo ◽  
Liver Extract ◽  
Functional Protein ◽  
Liver Cancer Cells ◽  
Expression Of Genes ◽  
Embryo Liver ◽  
The Impact

Abstract Background: The extracellular matrix (ECM) is a mosaic of various structural and functional proteins that cooperate with the cell, regulate adhesion, and consequently manage its further fate. Liver destruction is accompanied by a disruption of the physicochemical properties of the ECM which deregulates the cell-ECM interaction and can lead to uncontrolled proliferation and neoplastic transformation of cells. Therefore, it can be assumed that ECM modification and restoration of its characteristics for healthy tissue may counteract uncontrolled cell proliferation. The purpose of the presented research model was to optimise the physical characteristics of ECM by introducing a graphene oxide plane/nanofilm (nfGO) and enriching the cell environment with potentially missing proteins by adding a functional protein cocktail (chicken embryo liver extract) and determine the impact of these factors on cell-ECM cooperation and its consequences on adhesion, proliferation, and cell phase, which are factors of the invasiveness of cancer cells.Results: Experiments were performed with non-cancer HS-5 cells and liver cancer cells HepG2 and C3A. The cells were divided into four groups; (1) control, (2) cultured on GO nanofilm, (3) cultured with the addition of chicken embryo liver extract (CELE) to the medium and (4) cultured on the GO nanofilm with the addition of CELE. CELE contained 1735 proteins; the top 57 of these proteins have been presented. The use of GO nanofilm as well as CELE and nfGO + CELE reduced the proliferation of HepG2 cancer cells to the greatest extent; this is in contrast to non-cancer cells and also to C3A cancer cells. Furthermore, the combined use of the CELE protein cocktail and GO substrate effectively resulted in a decrease in the population of HepG2 cells in the G0/G1 phase and an increase of the population in G2/M. Molecular analysis of HepG2 cancer cells also showed an increase in the expression of genes responsible for adhesion such as fak (focal adhesion kinase), e-cadherin, and n-cadherin and a decrease in β-catenin, which is considered a proto-oncogen. Conclusions: Studies have shown that both the GO surface structure on which the cells are grown as well as the presence of a multi-component natural cocktail of regulatory proteins, can modify the expression of integrins, increase adhesion and, as a consequence, proliferation and the cell cycle - entering the resting phase. For the first time, it has been documented that hepatic cancer cells of the HepG2 line under the influence of stimuli derived from mimic ECM (graphene oxide) in interaction with a unique protein complex derived from chicken liver embryo decreased of the invasiveness of cancer cells.

scholarly journals Effect of lncRNA‑BC200 on proliferation and migration of liver cancer cells in�vitro and in�vivo

2019 ◽  
Author(s):  
Ni Tan ◽  
Bo Zhu ◽  
Hong Shu ◽  
Yi‑Feng Tao ◽  
Jun‑Rong Wu ◽  
...  
Keyword(s):  
Liver Cancer ◽  
Cancer Cells ◽  
Liver Cancer Cells ◽  
And Migration ◽  
Proliferation And Migration
Sign in / Sign up

Export Citation Format

Share Document